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1.
The administration of melatonin via intravaginal sponges is an effective method of advancing the breeding season in ewes. In the present study the fertility of melatonin-treated ewes has been compared with that of ewes induced to ovulate by conventional treatment. On June 25, 15, 15-month-old ewes (group 1) were given intravaginal implants containing melatonin in silastic tubing. On August 8, 13 similar ewes (group 2) were given Veramix sponges which were removed 12 days later, when they were given 500 iu pregnant mare's serum gonadotrophin (PMSG). Two intact raddled rams were introduced to the combined groups on August 21. The mean date of mating was September 3 +/- 1.5 for group 1 and August 21 +/- 0.2 for group 2 ewes. All the ewes in group 1 and 10 in group 2 (77 per cent) were mated. All the ewes were slaughtered approximately 50 days after mating and their reproductive tracts removed. The mean ovulation rates were 2.1 and 2.3 in groups 1 and 2, respectively. The results indicate that conception rates of 87 per cent and 61.5 per cent of ewes put to the ram were obtained in the melatonin-treated and PMSG-treated groups, respectively. At slaughter the melatonin-treated group were found to have a mean of 1.47 live fetuses per ewe put to the ram and the PMSG-treated group a mean of 1.08. It can therefore be concluded that melatonin implantation is an effective method for the advancement of seasonal breeding in anoestrous sheep, and that the fertility achieved is at least as good as that given by conventional progestogen-PMSG treatment. 相似文献
2.
Ghazali Muhamad Ezral Bin Argo Yanuar Kyotoh Harumichi Adachi Yasuhisa 《Paddy and Water Environment》2020,18(2):309-316
Paddy and Water Environment - In this study, we focused on the effect of the degree of floc growth on the moving velocity of the interface between sediment of flocculated montmorillonite and... 相似文献
3.
Kathryn M. Santos Paul R. Fisher Thomas H. Yeager Eric H. Simonne Hannah S. Carter William R. Argo 《Journal of plant nutrition》2013,36(10):1424-1436
Fertilization strategies during stock plant and cutting production are linked in terms of cutting nutrient levels and quality. Objectives were to evaluate (1) the effect of stock plant nutrition on tissue nutrient concentration and growth during vegetative propagation and (2) response to fertilizer during propagation for cuttings with 4 different initial tissue nutrient concentrations. ‘Supertunia Royal Velvet’ petunia stock plants were grown under constant fertigation of 0, 50, 100 or 200 mg nitrogen (N).L?1 for 21 days. The 200 mg N.L?1 solution contained 150 nitrate (NO3-N), 50 ammonium (NH4-N), 24 phosphorus (P), 166 potassium (K), 40 calcium (Ca), 20 magnesium (Mg), 0.7 sulfur (S), 1.0 iron (Fe), 0.5 manganese (Mn), 0.5 zinc (Zn), 0.24 copper (Cu), 0.24 boron (B), and 0.1 molybdenum (Mo). Providing a complete fertilizer during propagation of petunia, beginning immediately after sticking of cuttings, reduces the risk of nutrient deficiency. Particularly in situations where fertilizer is not applied early during propagation, stock plants should be managed to ensure unrooted cuttings have adequate nutrient reserves. 相似文献
4.
Peake KA Biggs AM Argo CM Smith RF Christley RM Routly JE Dobson H 《The Veterinary record》2011,168(11):301
A total of 318 cows were monitored in the pre-breeding postpartum period for the presence of three production stressors: lameness, subclinical mastitis and body condition score (BCS) loss. For each stressor, cows were given a classification of severely, moderately or non-affected based on mobility scores, somatic cell counts and BCS change. The number of days from calving to onset of the first luteal phase was greater in cows that had one severe production stressor (median 44 days) or two moderate production stressors (41 days) compared with cows that had no stressors (31 days) (P=0.02 and P=0.04, respectively). More than one severe stressor increased the interval further. There was no difference between cows with one moderate stressor (median 38 days) and those with none (P=0.13). The delay to the first luteal phase was significantly longer in cows with two moderate stressors if the onset of one stressor occurred at the time when resumption of ovarian activity was expected. The presence of these production stressors in early lactation had no effect on the interval from calving to establishment of the next pregnancy or the number of inseminations required despite the negative effect on the onset of the luteal phase. 相似文献
5.
Dugdale AH Curtis GC Cripps PJ Harris PA Argo CM 《Veterinary journal (London, England : 1997)》2011,(3):329-337
Changes in appetite, body mass (BM), body condition score (BCS), direct (ultrasonographic) and indirect (deuterium oxide dilution technique) measures of body fat were monitored in Welsh Mountain pony mares (n = 11, 5–19 years of age) offered ad libitum access to a complete diet (gross energy 16.9 ± 0.07 MJ/kg dry matter) for 12 weeks during summer (n = 6; 246 ± 20 kg) and winter (n = 5; 219 ± 21 kg). At the outset, each group comprised two thin (BCS 1–3/9), moderate (BCS 4–6/9) and obese (BCS 7–9/9) animals.For ponies that were non-obese at the outset, BM was gained more rapidly (P = 0.001) in summer (0.8 ± 0.1 kg/day) than winter (0.6 ± 0.0 kg/day). This was associated with a seasonal increase in dry matter intake (DMI) which became maximal (summer, 4.6 ± 0.3% BM as DMI/day; winter, 3.5 ± 0.1% BM as DMI/day) during the second month. The appetite of the obese ponies was half that reported for non-obese animals in the summer and BM remained constant irrespective of season.Body ‘fatness’ increased progressively for non-obese but not obese ponies. Body fat content was exponentially associated with increasing BCS but BCSs >6 were not useful indicators of actual body fat. Endogenous circannual mechanisms to suppress winter weight gain were insufficient to prevent the development of obesity in ad libitum fed ponies. 相似文献
6.
Jinsheng Huang Paul R. Fisher W. E. Horner William R. Argo 《Journal of plant nutrition》2013,36(6):846-858
The objective was to quantify how the concentration and particle size of unreacted “residual” limestone affected pH buffering capacity for ten commercial and nine research container substrates that varied in residual calcium carbonate equivalents (CCE) from 0.3 to 4.9 g CCE·L?1. The nine research substrates contained 70% peat:30% perlite (by volume) with dolomitic hydrated lime at 2.1 g·L?1, followed by incorporation of one of four particle size fractions [850 to 2000 μm (10 to 20 US mesh), 250 to 850 μm (20 to 60 US mesh), 150 to 250 μm (60 to 100 US mesh), or 75 to 150 μm (100 to 200 US mesh)] of a dolomitic carbonate limestone at 0, 1.5 or 3.0 g·L?1. Substrate-pH buffering was quantified by measuring the pH change following either (a) mineral acid drenches without plants, or (b) a greenhouse experiment where an ammonium-based (acidic) or nitrate-based (basic) fertilizer was applied to Impatiens wallerana Hook. F. Increasing residual CCE in commercial substrates was correlated with greater pH buffering following either the hydrochloric acid (HCl) drench or impatiens growth with an ammonium-based fertilizer. Research substrates with high applied lime rate (3.0 kg·m?3) had greater pH buffering than at 0 or 1.5 g·L?1. At 3 g·L?1, the intermediate limestone particle size fractions of 250 to 850 μm and 150 to 250 (20 to 60 or 60 to 100 US mesh) provided the greatest pH-buffering with impatiens. Particle fractions finer than 150 μm reacted quickly over time, whereas buffering by particles coarser than 850 μm was limited because of the excessively slow reaction rate during the experimental periods. Addition of acid from either an ammonium-based fertilizer or HCl reduced residual CCE over time. Dosage with 40 meq acid from HCl per liter of substrate or titration with HCl acid to substrate-pH of 4.5 were well-correlated with pH buffering in the greenhouse trials and may be useful laboratory protocols to compare pH buffering of substrates. With nitrate fertilizer application, residual CCE did not affect buffering against increasing pH. Residual limestone is an important substrate property that should be considered for pH management in greenhouse crop production under acidic conditions. 相似文献
7.
Reasons for performing study: Evaluation of equine body fat content is important for nutritional and clinical purposes. However, our understanding of total body fat and its regional distribution in the body is sparse. Currently, body fat evaluation relies on the subjective assessment of body condition score (BCS), which has never been validated against ‘gold standard’ chemical analysis or dissection measurements in ponies. Objectives: To define the relationships between subjective (BCS), objective (morphometric) indices of body fat and ‘gold standard’ measurements of actual body composition. Hypotheses: BCS and morphometry offer valid, noninvasive methods for determination of body fat in equids. Methods: Seven mature (mean ± s.e. 13 ± 3 years, 212 ± 14 kg, BCS 1.25–7/9), Welsh Mountain pony mares, destined for euthanasia (for nonresearch purposes), were used. For all ponies, body mass (BM), BCS and various morphometric measurements were recorded. Following euthanasia, all ponies were systematically dissected. Discrete white adipose tissue (WAT) depots were independently described. Gross, body chemical composition was determined by proximate analyses. Results: Total somatic soft tissues increased linearly (r2= 1.00), whereas body WAT content (1–26% live BM) increased exponentially (r2= 0.96), with BCS. WAT was equally distributed between internal and external sites in all animals irrespective of BCS. Nuchal fat was a poor predictor of total WAT (r2= 0.66). Periorbital WAT did not alter with BCS (r2= 0.01). Heart girth:withers height and ultrasonic retroperitoneal fat depth were closely associated with total, chemically‐extracted lipid which comprised 1–29% live BM (r2= 0.91 and 0.88, respectively). Conclusions and potential relevance: The exponential relationship between BCS and total body WAT/lipid suggests that BCS is unlikely to be a sensitive index of body fat for animals in moderate‐obese states. Morphometric measurements (body girths and retroperitonel fat depth) may be useful to augment subjective BCS systems. 相似文献
8.
Reasons for performing the study: Excessive accumulations or depletions of body fat have been associated with increased morbidity and mortality in horses and ponies. An objective, minimally‐invasive method to accurately quantify body fat in living animals is required to aid nutritional management and define welfare/performance limits. Objectives: To compare deuterium oxide (D2O) dilution‐derived estimates of total body water (TBW) and body fat with values obtained by ‘gold standard’ proximate analysis and cadaver dissection. Hypothesis: D2O dilution offers a valid method for the determination of TBW and body fat in equids. Methods: Seven mature (mean ± s.e. 13 ± 3 years, 212 ± 14 kg, body condition scores 1.25–7/9), healthy, Welsh Mountain pony mares, destined for euthanasia (for nonresearch purposes) were used. Blood samples were collected before and 4 h after D2O (0.11–0.13 g/kg bwt, 99.8 atom percent excess) administration. Plasma was analysed by gas isotope ratio mass spectrometry following filtration and zinc reduction. After euthanasia, white adipose tissue (WAT) mass was recorded before all body tissues were analysed by proximate chemical analyses. Results: D2O‐derived estimates of TBW and body fat were strongly associated with proximate analysis‐ and dissection‐derived values (all r2>0.97, P≤0.0001). Bland‐Altman analyses demonstrated good agreements between methods. D2O dilution slightly overestimated TBW (0.79%, limits of agreement (LoA) ‐3.75–2.17%) and underestimated total body lipid (1.78%, LoA ‐0.59–4.15%) and dissected WAT (0.72%, LoA ‐2.77–4.21%). Conclusions and potential relevance: This study provides the first validation of the D2O dilution method for the minimally‐invasive, accurate, repeatable and objective measurement of body water and fat in living equids. 相似文献
9.
Shahani SK Revell SG Argo CG Murray RD 《Pakistan journal of biological sciences: PJBS》2010,13(16):802-808
Recently positive correlation has been found between oxygen consumption (ZO2) in bull spermatozoa and non-return rates and concluded that an increase in ZO2, characteristic of the freeze/thaw process, was possibly associated with mitochondrial membrane damage during this procedure: alternatively, sperm may be hyperactivated through the capacitation-like effects of freezing/thawing. We speculated that the morphology of spermatozoa may be associated with their rate of ZO2 and fertility: for example, sperm mid-piece length where mitochondria are located. Such a relationship has not been investigated before, particularly in context of commercial cattle breeding programmes and bull fertility characteristics. Sperm biometry was performed on ejaculates obtained from 34 bulls representing six breeds: Holstein (yearlings and mature), Friesian, Belgian Blue, Aberdeen Angus, Charolais and Limousin. Five ejaculates were collected from every bull and from each sample a semen smear was fixed and stained with eosin/nigrosin: the mid-piece length of 40 sperm with normal morphology was measured in every sample. Data were analysed by breed, age and within each bull. Significant differences (p<0.01) between ejaculates in 9/34 bulls was found, as well as differences (p<0.001) between individual bulls within the same breed. The average mid-piece length for Aberdeen Angus was 13.35 microm, for Belgian Blues and Limousin around 13.8 microm and for Charolais 13.68 microm: for dairy breeds such as Holstein and Friesian it was about 13.4 microm. The mean value of mid-piece length for breed was compared with their 49 day non-return rate; a negative correlation (r = -0.53) was found in black and white dairy breeds. 相似文献
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