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1.
Identification of genetic factors controlling traits associated with seed germination under drought stress conditions, leads to identification and development of drought tolerant varieties. Present study by using a population of F2:, derived from a cross between a drought tolerant variety, Gharib (indica) and a drought sensitive variety, Sepidroud (indica), is to identify and compare QTLs associated with germination traits under drought stress and non-stress conditions. Through QTL analysis, using composite interval mapping, regarding traits such as germination rate (GR), germination percentage (GP), radicle length (RL), plumule length (PL), coleorhiza length (COL) and coleoptile length (CL), totally 13 QTLs were detected under pole drought stress (-8 MPa poly ethylene glycol 6000) and 9 QTLs under non-stress conditions. Of the QTLs identified under non-stress conditions, QTLs associated with COL (qCOL-5) and GR (qGR-1) explained 21.28% and 19.73% of the total phenotypic variations, respectively Under drought stress conditions, QTLs associated with COL (qCOL-3) and PL (qPL-5) explained 18.34% and 18.22% of the total phenotypic variations, respectively. A few drought-tolerance-related QTLs identified in previous studies are near the QTLs detected in this study, and several QTLs in this study are novel alleles. The major QTLs like qGR-1, qGP-4, qRL-12 and qCL-4 identified in both conditions for traits GR, GP, RL and CL, respectively, should be considered as the important and stable trait-controlling QTLs in rice seed germination. Those major or minor QTLs could be used to significantly improve drought tolerance by marker-assisted selection in rice.  相似文献   
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Applying by-products as soil amendments to agricultural systems is growing in popularity. We aimed to assess the efficacy of some contemporary by-products to provide nutrients to crops as well as the potential harm of adding toxic elements to the environment. Four different by-products widely available in Northern Europe were tested for their effects on two nutrient-poor agricultural soils in terms of increasing available macro- and micro-nutrients as well as toxic elements. Assessing soil microbial community as a sensitive tool for evaluating soil quality was conducted with the focus on microbial activity, carbon metabolism and on Rhizobium/Agrobacterium. Wood ash increased pH and CaEDTA, KEDTA and MgEDTA in the soils. The only increase in EDTA-extractable micronutrients in the soils was observed by applying pot ale, increasing Cu. None of the amendments increased the availability of Pb and Cd in the soils. Soils amended with the by-products thus remained similar to the unamended control but were quite different from fully mineral fertilised soils. There were no detectable adverse effects on the physiological and genetic profiles of microbial communities. The by-products were moderately beneficial and did not change the soil microbial community as much as the fully fertilised treatment with mineral fertilisers. Changes in the microbial community profiles were probably due to direct effects on microbes limited by K, Ca and N as opposed to indirect effects on plant growth. This is potentially significant in understanding how to improve impoverished and marginal soils as microbial activity affects many other ecosystem functions.  相似文献   
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This study was carried out to evaluate the antioxidative potential and quality of the meat of African catfish fed mulberry foliage extract (MFE). A total of 360 juvenile African catfish (8.4 ± 0.2 g) were fed four diets namely, basal diet (control), MFE‐2 (2 g MFE kg?1), MFE‐5 (5 g MFE kg?1) and MFE‐7 (7 g MFE kg?1) for 60 days. At the end of the experiment, muscles were excised, vacuum‐packaged and conditioned for 0, 7 and 14 days in a chiller (4°C). The meat from fish fed MFE‐5 and MFE‐7 had significantly (P < 0.05) greater total phenols content than the other dietary groups. This value reduced (P < 0.05) during storage. The DPPH‐scavenging effect of MFE‐7 increased (P < 0.05) compared with the others. It decreased (P < 0.05) during storage. The lowest POV was revealed (P < 0.05) in MFE‐7. The 2‐thiobarbituric acid‐reactive substances (TBARS) and peroxide value (POV) increased (P < 0.05) during storage. The pH value was significantly (P < 0.05) higher in MFE supplemented diets than in the control group. It is concluded that MFE at the concentration of 7 g kg?1 DM is potential dietary antioxidant supplements, to improve the quality of fish meat.  相似文献   
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Seed vigour is one of the most important parameters for establishing rice seed that affects high yielding production, especially under stress condition in rice (Oryza sativa L.). Improvement of rice yields under abiotic stress through breeding requires a good understanding of genetic factors controlling important traits such as seedling vigour and related traits. To understand the genetic background of the relationships among these traits in indicia rice, we genetically analyzed F2:3 population derived from a cross between Tarommahalli × Khazar, under osmotic stress caused by sorbitol. In this investigation, a simple sequence repeat (SSR) linkage map of 1231.50 cM of rice was constructed by using 74 markers and quantitative trait loci (QTL) analysis was accomplished for eight traits including germination percent, seed germination rate, radicle length, plumule length, coleoptile length, radicle dry matter, plumule dry matter, and coleoptile dry matter. A total of 32 QTLs were identified for the eight traits. Among them, one QTL for percent of germination (qGP-8) was located in RM6208-8264 on chromosome 8 showed large effect (28.99%) on the trait. These results suggest the potential for increase productivity by transferring osmotic stress tolerance gene from Tarommahalli to other rice lines and identification of QTLs with large effects could be considered for use in marker assisted selection (MAS).  相似文献   
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Administration of probiotic candidates in fish has generally been shown as a useful strategy to improve growth performance, survival, digestive enzyme activity, and gut microbiota. Unfortunately, the sero-immunological responses of different fish to different probiotic candidates are poorly understood. The present study assessed the effect of Pediococcus acidilactici as a probiotic on the biochemical and immunological parameters of beluga. Fish (248.32?±?10.21 g) were fed a control diet (without P. acidilactici( and three different doses of P. acidilactici-supplemented diets (107, 108, and 109 CFUg?1 diets) for 8 weeks. On week 8, blood and serum were sampled. Dose-dependent increase of immunological parameters (respiratory burst activity, lysozyme content, serum antibacterial activity, and total immunoglobulin) and biochemical parameters (total protein and albumin levels) was observed. However, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly increased in the juvenile beluga fed by 109 CFUg?1 P. acidilactici-supplemented diet compared to the other groups. Based on the results of this evaluation, it is reasonable to conclude that the inclusion of P. acidilactici as probiotic in diets for juvenile beluga improves the sero-immunological parameters of the fish and should be considered by farmers as a strategy to improve fish health.  相似文献   
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In order to study selection indices for improving rice grain yield, a cross was made between an Iranian traditional rice (Oryza sativa L.) variety, Tarommahalli and an improved indica rice variety, Khazar in 2006. The traits of the parents (30 plants), F1 (30 plants) and F2 generations (492 individuals) were evaluated at the Rice Research institute of Iran (RRII) during 2007. Heritabilities of the number of panicles per plant, plant height, days to heading and panicle exsertion were greater than that of grain yield. The selection indices were developed using the results of multivariate analysis. To evaluate selection strategies to maximize grain yield, 14 selection indices were calculated based on two methods (optimum and base) and combinations of 12 traits with various economic weights. Results of selection indices showed that selection for grain weight, number of panicles per plant and panicle length by using their phenotypic and/or genotypic direct effects (path coefficient) as economic weights should serve as an effective selection criterion for using either the optimum or base index.  相似文献   
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Asparagus officinalis is the most economically important species of asparagus worldwide. Commercial cultivars of asparagus are susceptible to some biotic and abiotic stresses. Drought stress is one of the most common stresses in agricultural crops cultivation. The main purpose of the current study was to compare Iranian asparagus accessions drought tolerance rate with that of ‘Mary Washington’ commercial cultivar and introducing superior accessions in terms of dry resistance rate. A factorial experiment was laid out based on a completely randomized design with three replications. The factors were a) asparagus accession in 10 levels including nine wild accessions of Iranian asparagus and ‘Mary Washington’ commercial cultivar (as control) and b) drought stress in four levels including 0 (as control), 5, 10 and 15% (w/v polyethylene glycol). Results showed that highest values of percentage and speed of seed germination and lengths of radicle and plumule were recorded in Gazanak accession. The lowest values of radicle length were recorded in Chalaki and Shiraz accessions (6.19 and 8.01?mm, respectively). Calli of ‘Mary Washington’ cultivar under highest applied level of drought stress showed the lowest value of relative growth rate (11.53?mg/day). Cluster analysis based on assessed characteristics under different applied levels of drought stress, divided Iranian asparagus accessions into four groups at Euclidean distance of 2.0. In conclusion, obtained results showed that Iranian asparagus accessions especially Gazanak accession could be a good candidate to apply in asparagus breeding programs with aim of producing asparagus dry tolerant cultivars.

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