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1.
Twenty dogs and 29 cats were identified with urethral rupture. Males predominated in both groups. The most common cause of urethral rupture in dogs was vehicular trauma, and in cats it was trauma associated with urethral obstruction and catheterization. Clinicopathological findings, type of surgical correction, time to surgery, type of urinary diversion, and duration of urinary diversion were not statistically associated with the outcome. In this study, the presence of multiple traumatic injuries was associated with a poor outcome.  相似文献   
2.
The compensatory growth response of the European sea bass (Dicentrarchus labrax) that faced cycling starvation and restricted ration was assessed. Juveniles (10.5 g) were stocked into 15 tanks at a density of 25 fish per tank. Five different feeding regimes were tested on triplicate groups of fish: CSatiation: control fed for 60 days without deprivation, CRestricted: 25% restricted feeding, S‐R: 1 day starvation then 4 days CRestricted feeding, R‐F: 1 day CRestricted feeding then 4 days CSatiation feeding, and, finally, S‐F: 1 day starvation then 4 days CSatiation feeding. The specific growth rate of fish in the CSatiation (2.5 ± 0.06% day?1), S‐F (2.5 ± 0.11% day?1) and R‐F (2.4 ± 0.18% day?1) were significantly higher than that of CRestricted (2.2 ± 0.05% day?1) or S‐R (2.0 ± 0.01% day?1). Fish in S‐F group were able to achieve catching up with the CSatiation. There was no significant difference in feed conversion rates but R‐F and S‐F consumed approximately 34% more feed than CSatiation following the first re‐feeding day. Although, the highest lipid content was observed in CSatiation (14.4%), S‐R (33.3% dry matter) had the highest water content compared with the CSatiation and CRestricted (37.8% and 36.9% dry matter respectively). In conclusion, it may be concluded that sea bass has rapid response to cycling starvation/re‐feeding and that a 25% restricted feeding ratio is insufficient to invoke a compensatory growth response in sea bass.  相似文献   
3.
Cultivated wheat, (Triticum aestivum L.), is one of the most important food crops in the world. The Aegilops L. genus is frequently utilized by plant breeders for improving the current wheat cultivars due to their close relationships. Therefore, understanding the phylogenetic relationships among the species of these genera is not only valuable for plant taxonomy, but also for plant breeding efforts. The presented phylogenetic analysis was based on the sequences of trnT-F chloroplast DNA containing three non-coding sub-regions. Twelve genotypes belonging to four species of Triticum L. genus and twenty-four genotypes belonging to eight species of Aegilops genus were used in the current study. The results postulated a close genetic relationship between diploid Aegilops species containing the BB genome and polyploid Triticum species. With the exception of Aegilops cylindrica Host (CCDD), all other Aegilops species having the CC genome were alienated from Aegilops speltoides Tausch (BB) and clustered together. These two clusters joined by a third cluster including the AA genome containing diploid Triticum species.  相似文献   
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5.
The influence of a humic deposit (Gyttja, G) alone (applied at 25 kg ha−1) and in combination with mineral fertilizer (G + NP) on soil organic matter content, pH, electrical conductivity, total N content, calcium carbonate content, enzyme activities (urease, β-glucosidase, arylsulphatase, and alkaline phosphatase), microbial biomass C, soil respiration, and availability of Cd, Pb, Ni, and Zn was examined through a 180-day incubation period and compared with the behavior of no treatment (control) and NP treatment. A significant increase in organic matter content was observed in soils treated with G + NP. Compared with G and NP alone, the G + NP-amended soils showed higher values of the selected microbiological properties.Diethylenetriaminepentaacetic-acid-extractable Cd, Pb, Ni, Cu, and Zn increased significantly with increasing rates of NP, but the addition of G + NP resulted in a considerable decrease in the amount of extractable metals during the incubation period (P<0.05). Based on these results, it can be concluded that the organic matter applied in the gyttja led to an increase in the metal adsorption capacity of the amended soils. This material can be used to reduce the availability and mobility of heavy metals in the soils intensively amended with mineral fertilizers. A combination of G with NP can, therefore, be considered as an alternative approach in the applications of organomineral fertilization.  相似文献   
6.
The effects of increased dietary sodium chloride supplement on growth, feed efficiency (FE), feed dispensed rate and fillet composition and morphological indices of European sea bass (initial weight of 4.9 g) were investigated in freshwater using five different levels of supplemental salt (0 (control diet, with no salt supplemented), 1%, 3%, 5% and 9% of diet). Over the experimental period of 50 days, the final weight of dietary salt (DS) groups was found to be approximately 19% higher than the control group. The final weight, specific growth rate and FE were greatest at the moderate level (1–5%) of salt supplementation. Optimum level of DS inclusion was calculated as 3%. Feed efficiency was significantly enhanced with increasing salt level up to 5%. A negative relationship between daily feed intake and FE (r2=0.66) was observed. The average muscle ratio (MR) in groups fed 1–5% supplemented salt was around 51.4% whereas fish receiving control and 9% salty diets had 5.3% lower MR (48.7%) (P<0.05). However, no significant effects of salt inclusion were found on viscera or hepatosomatic indices. Muscle ash and dry matter content slightly rose with increasing DS in the diet, but with no statistical difference (P>0.05). Similarly, no significant changes were observed in either muscle protein or lipid content among the groups (P>0.05).  相似文献   
7.
The prevalence of enterococci in neck skin samples of poultry from Ankara region in Turkey was investigated and their antibiotic resistance patterns were determined. In the study, 83 of 106 analyzed neck skin samples were positive for Enterococcus, with E. faecium as the most prevalent species (48%) followed by E. durans (23%) and E. faecalis (19%). Lower numbers were detected for E. gallinarum, E. hirae, E. mundtii and E. casseliflavus. Using the disc diffusion method, it was established that over 90% of E. faecium and E. faecalis isolates were high-level resistant against erythromycin and tetracycline. Four E. faecium isolates were additionally resistant to chloramphenicol, gentamicin and streptomycin, though they were susceptible to penicillin G. The most frequently observed multiple resistance in E. faecium (25%) was against erythromycin, tetracycline, chloramphenicol and streptomycin. Of the E. faecalis isolates, 44% were multiple resistant to erythromycin, tetracycline and streptomycin. Vancomycin resistance could not be demonstrated phenotypically and vanA or vanB genes were not detected by multiplex PCR in any of the isolates. Nevertheless, the observed resistance patterns are of concern for public health.  相似文献   
8.
Objective: To describe in vitro elution characteristics of amikacin and vancomycin from calcium sulfate hemihydrate 98% (plaster of Paris, POP) beads and characterize eluent inhibition of Staphylococcus spp. Study Design: Experimental study. Methods: POP beads were impregnated with amikacin or vancomycin alone or in combination and then incubated alone or in combination for 84 days at 37°C in plastic tubes containing sterile phosphate‐buffered saline (PBS). Beads containing no antimicrobial served as negative control. Beads were intermittently moved to a new tube containing drug‐free PBS. Antimicrobial was measured in the eluent using a polarized fluorescent immunoassay. Eluent inhibition of Staphylococcus spp. was determined at each time point. Results: Antimicrobial release from beads was characterized by an initial rapid phase then a slower phase. Although antimicrobial release from beads occurred throughout the 84 days, most was in the first 24 hours, except for vancomycin alone. Duration of eluent inhibition of Staphylococcus spp. growth ranged from 0.5 (amikacin alone) to 56 days (vancomycin alone). Control eluent did not inhibit bacterial growth. Conclusions: Amikacin elution from POP beads was rapid, inhibiting growth for <24 hours with or without vancomycin. Vancomycin elution was slower and inhibited growth for 56 days alone or for 5 days with amikacin. Clinical Relevance: Vancomycin‐impregnated beads appear to be reasonable as a therapeutic option whereas amikacin‐impregnated POP beads and amikacin and vancomycin combinations may require further study before considering as a therapeutic option.  相似文献   
9.
OBJECTIVES: To compare combined vacuum and rotation with the spinner flask technique for seeding chondrocytes on chitosan versus polyglycolic acid matrices. SAMPLE POPULATION: Porcine chondrocytes. PROCEDURE: A suspension containing 5 X 10(6) chondrocytes/scaffold was used to evaluate 2 seeding techniques, including a spinner flask and a custom-designed vacuum chamber used for 2 hours prior to transfer to a bioreactor. For each seeding technique, prewetted scaffolds were composed of polyglycolic acid (PGA) mesh or macroporous chitosan sponge. Constructs were collected at 48 hours for DNA quantification, measurement of water and gycosaminoglycan (GAG) content, and scanning electron microscopy. RESULTS: Yield of both seeding techniques was similar for each type of scaffold. Percentage of cells contained in the center of PGA constructs was increased with seeding in the bioreactor (43% of total cell number), compared with the spinner flask (18%).The DNA content and cell number per construct were 10 times greater for PGA constructs, compared with chitosan constructs. Chitosan scaffolds seeded in the bioreactor yielded a significantly higher GAG:DNA ratio than did PGA scaffolds. Whereas chondrones formed on chitosan scaffolds, cell distribution was more uniform on PGA scaffolds. CONCLUSIONS AND CLINICAL RELEVANCE: The vacuum-bioreactor technique allowed seeded chondrocytes to attach to PGA scaffolds within 48 hours and improved uniformity of cell distribution, compared with the spinner technique. Although formation of extracellular matrix may be stimulated by seeding chitosan scaffolds in the bioreactor, further evaluations of the seeding technique and characteristics of chitosan scaffolds are warranted.  相似文献   
10.
Manganese was removed from naturally polluted river sediment by applying an electrokinetic remediation technique. The sediment was alkaline and had 20% clay, which was mainly illite. The electrokinetic remediation experiments were performed by controlling pHs in the electrode cells and reverse electroosmotic flows were observed, i.e., water moved from cathode towards anode. Manganese accumulated in areas closer to cathode, however, other metals, such as copper, zinc and lead were mostly observed in the middle section of the sediment. As a result of reverse electroosmotic flow, the removal efficiencies of metals were low and the highest removal efficiencies of manganese, copper and lead, were evaluated as 18%, 20% and 12%, respectively. Almost no removal of zinc was observed in all electrokinetic remediation experiments.  相似文献   
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