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The attenuation of a Babesia bovis strain depends on its protease content. The present work evaluates this parameter on a virulent strain, before and after attenuation by quick passages on splenectomized calves. The protease activity at different pH values was determined in protein fractions from the blood of calves. The enzymatic test showed marked differences between the protease content of both substrains.  相似文献   
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This study was aimed at characterizing the anthocyanins and phenolics profile in different varieties of pigmented corn and wheat and in some of their milling fractions. Acid/ethanol extracts were used to assess total anthocyanins, overall antioxidant activity, the overall polyphenol profile, and for evaluating the inhibition of pancreatic α-amylase and of intestinal α-glucosidase. Both enzymes were inhibited in a dose-dependent manner by all extracts, but individual extracts had specific effects on each enzyme. Anti-inflammatory response was evaluated by using acid-free extracts and Caco-2 cells transiently transfected with a luciferase reporter gene responding to cytokine stimulation. The immune response of interleukin-stimulated cells decreased significantly in a dose-dependent manner in the presence of 20–50 μM/l anthocyanins from all grains extracts, again with a different efficiency. The inhibitory ability and the anti-inflammatory capability of these extracts are in most cases higher than in similar extracts from other sources, suggesting that activities in each extract may imply specific synergies between anthocyanins and other phenolics.

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Field experiments were set up from 2001 to 2004 in North West Italy to determine the effects of plant density on the susceptibility of medium and medium-late maturity maize hybrids to ear rot and to mycotoxin contamination in natural infection conditions. The ears were rated for the incidence and severity of disease symptoms at harvest and the harvested kernels were analysed for mycotoxin fumonisin B1, zearalenone, deoxynivalenol, aflatoxin B1 and ochratoxin A.  相似文献   
4.
The antifungal activity of methanolic extract and alkaloidal fraction of Berberis aetnensis against Candida species was investigated. The crude extract was active against Candida species, this activity being higher than that of the alkaloidal fraction and berberine.  相似文献   
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Journal of Pest Science - Sunn pests are responsible for serious kernel damage on wheat (Triticum aestivum L., Poaceae), affecting bread-making quality from their feeding at the late milk-ripe...  相似文献   
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Fusarium verticillioides is a common maize pathogen which causes diseases on ears and synthesis of the mycotoxins, fumonisins, in kernels. Fumonisin production is influenced by both environmental conditions and agricultural inputs during growth and maturation of the maize plant. The aim of this research was to evaluate the effect of crop management techniques on fumonisin contamination in maize kernels. Experiments were conducted in Northern Italy during 2006 and 2007 using two maize hybrids with different precocity. Factors evaluated were seed planting time (March versus May), plant density (65,000 plants versus 80,000 plants per ha), N fertilization (200 kg N versus 400 kg N per ha), and chemical treatment to control European maize borer (ECB) (yes versus no). These factors were analyzed were as follows: (T1) May sowing, high plant density and N fertilization, (T2) March sowing time, high plant density and N fertilization, (T3) March sowing time, low plant density and balanced N fertilization, (T4) the same agronomic techniques as T3 plus a chemical treatment to control European maize borer. Ears were analyzed morphologically for ECB incidence and severity. Subsequently, kernels were analyzed chemically for fumonisins. Fumonisin contamination was reduced by 57%, 69% and 86% for T2, T3 and T4, respectively, compared to T1. This study clearly underlines that the application of good agricultural practices (GAP) in crop management strategies can effectively control fumonisin contamination of maize kernels.  相似文献   
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Since the production of durum wheat in the drier areas of the Mediterranean Basin is characterized by high variability in terms of yield and grain quality, there is also considerable interest in developing durum wheat in the northern regions, where the pedo-climatic conditions can offer the possibility of obtaining grain yields with higher technological quality and stability. However, the climatic conditions in the northern regions make durum wheat more prone to fungal foliar disease, particularly to Septoria Tritici Blotch (Septoria tritici Rob.) and to Fusarium Head Blight (Fusarium graminearum Petch and Fusarium culmorum Sacc.), with the consequent occurrence of DON in grains.Field experiments have been conducted over two growing seasons at four sites in North West Italy to evaluate the effect of fungicides and foliar nitrogen fertilizer application on durum wheat yield and grain quality. Five combinations of foliar application were compared at each site and each year (untreated control, azole fungicide application at heading, strobilurin fungicide at the stem elongation stage and/or at heading, the addition of a foliar N fertilizer to a fungicide programme). The following parameters were analysed: Septoria Tritici Blotch (STB) severity, flag leaf greenness using a chlorophyll meter, grain yield, test weight, grain protein content, ash content, vitreousness, Fusarium Head Blight (FHB) incidence and severity and deoxynivalenol (DON) contamination. The collected data underline that the cultivation of durum wheat at the climatic conditions of North Italy is actually risky and needs a direct control of fungal disease, which would be able to reduce the development of both foliar and head attacks. The double treatment, with a strobilurin application during the stem elongation stage and azole at heading, results to be an essential practice and showed advantages in terms of the delay of flag leaf senescence (+27%), STB control (+31), FHB control (+11%), yield (+32%) and DON contamination (−45%), compared to the untreated control. Other foliar treatments at heading, such as strobilurin or foliar N fertilizer applications, do not seem to provide any further advantage, for either grain yield or quality. No significant effect of fungicide or foliar N fertilizer application was recorded on the protein or ash concentration or vitreousness.  相似文献   
8.
In this study, field experiments have been conducted over three growing seasons to evaluate the effect of fungicides (triazoles and strobilurins) and a foliar fertilizer application to winter wheat at anthesis on flag leaf senescence, grain yield, bread-making quality, Fusarium head blight (FHB) disease and deoxynivalenol (DON) contamination.Flag leaf greenness was significantly influenced by the fungicide application. A delay in flag leaf senescence following triazole use did not increase grain yield. No effects of fungicide application on protein concentration were recorded. The application of strobilurin to a triazole programme did not significantly delay senescence of the flag leaf or increased yield, compared to the triazole-only application, while a consistent benefit was observed for dough strength (W). The triazole application led to significantly lower FHB symptoms and DON contamination, while the triazole-strobilurin fungicides programme led to an increase in DON contents, that were often higher than the untreated controls.Grain yield and quality were improved when a foliar feed containing macro- and micro-nutrients was added to a triazole fungicide programme, with no additional risk for DON contamination. Compared to the strobilurin fungicide application, the foliar fertilizer led to a longer delay of the flag leaf senescence and higher bread-making quality.  相似文献   
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