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E.M. Oxford C.G. Danko P.R Fox B.G. Kornreich N.S. Moïse 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(1):92-101
Background
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited myocardial disease with high prevalence in the Boxer dog population. It is characterized by replacement of the myocardium with fatty or fibro‐fatty tissue. Several mechanisms for the development of ARVC have been suggested, including dysfunction of the canonical Wnt pathway, which is linked to many cellular functions, including growth and differentiation of adipocytes.Hypothesis
Wnt pathway dysfunction is involved in the development of ARVC in the Boxer as evidenced by mislocalization of β‐catenin, an integral Wnt pathway modulator, and striatin, a known Wnt pathway component.Animals
Five dogs without ARVC and 15 Boxers with ARVC were identified by 24‐hour Holter monitoring and histopathologic examination of the heart.Methods
Right ventricular samples were collected and examined using confocal microscopy, Western blots, and quantitative (q) PCR.Results
Confocal microscopy indicated that β‐catenin localized at sites of cell‐to‐cell apposition, and striatin localized in a diffuse intracellular pattern in hearts without ARVC. In hearts affected with ARVC, both β‐catenin and striatin were colocalized with the endoplasmic reticulum (ER) marker calreticulin. Western blots indentified a 50% increase in the amount of β‐catenin in ARVC samples. No change in β catenin mRNA was detected using qPCR.Conclusions
Our data suggest that trafficking of Wnt pathway proteins from the ER to their proper location within the cell is inhibited in Boxers with ARVC. These results suggest that disturbances in the Wnt pathway may play a role in the development of ARVC in the Boxer. 相似文献3.
Marcela Capcarova Eva Petrovova Marianna Dankova Jan Danko 《Pesticide biochemistry and physiology》2010,98(2):213-218
The aim of this work was to determinate the effect of carbamate insecticide bendiocarb (2,2-dimethyl-1,3-benzodioxol-4-yl N-methylcarbamate) on selected parameters of rabbit homeostasis. Animals were divided into four groups (control C, and experimental groups E1, E2 and E3 according to days of administration 10, 15 and 25 days). Animals from experimental groups received bendiocarb per os in a dose 5 mg/kg of body weight per day. Significant increase of creatinine content in E3 group (the longest administration of bendiocarb), increase of aspartate aminotransferase (E1 and E3 against control group) and gamma glutamyl transferase (E3 against control group) and decrease of glutamate dehydrogenase (E1 against control group) inform about possible failure of liver and/or kidney caused by bendiocarb. Decrease of haemoglobin (significantly in E3 group against control group), mean corpuscular haemoglobin (MCH) concentration (in all experimental groups), platelet count (in all experimental groups) can signify defection in haemoplastic system. 相似文献
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D. Mazensky J. Danko E. Petrovova E. Mechirova M. Prokes 《Anatomia, histologia, embryologia》2014,43(5):346-351
The aim of this study was to investigate the arterial blood supply of the thoracolumbar spinal cord in rabbit. The study was carried out on twenty adult New Zealand white rabbits. Ten rabbits were used in the corrosion technique and ten rabbits in the dissection technique. After the killing, the vascular network was perfused with saline. Batson's corrosion casting kit no. 17 © was used as a casting medium. After polymerisation of the medium, in ten rabbits the maceration was carried out in KOH solution, and in ten other rabbits, formaldehyde was injected by the dissection technique into the vertebral canal. We found high variability of segmental arteries supplying blood to the spinal cord. There are 12 intercostal arteries and 1 costo‐abdominal artery. Dorsal branches arising from the dorsal surface of the aorta thoracica were found as follows: in 70% of the cases, 9 pairs were present; in 20% of the cases 8 pairs; and in 10% of the cases 10 pairs. The paired arteriae lumbales were present in 6 pairs in 90% of the cases and in 5 pairs in 10% of the cases. On the dorsal surface of spinal cord, we found two irregular longitudinal arteries in 70% of the cases, no longitudinal arteries in 20% of the cases and three irregular longitudinal arteries in 10% of the cases receiving dorsal branches of rami spinales. Among the dorsal branches observed in the thoracic region, 60.5% were left‐sided, 39.5% right‐sided and in the lumbar region, 52.5% were left‐sided and 47.5% right‐sided. 相似文献
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Danko Abramiuc Luminita Ciobanu Rodica Muresan Magda Chiosac Augustin Muresan 《Fibers and Polymers》2013,14(11):1826-1833
The paper discusses a method to functionalize cotton fabrics using biologically active natural compounds to achieve the antibacterial characteristics required for medical application. The biologically active natural compounds include propolis, beeswax, and chitosan. Three 100 % cotton knitted fabrics with different degrees of compactness were impregnated in the emulsions containing the active ingredients and fabric variant G3 with the highest degree of impregnation was considered for the evaluation of the antibacterial properties and comfort characteristics. The results show that the treated cotton fabric had high antibacterial activity against both gram positive bacteria Staphylococcus aureus and Streptococcus β haemolytic, and gram negative bacteria Escherichia coli and Pseudomonas aeruginosa. The presence of the biologically active natural compounds on the cotton substrates modified the surface of the textile fibers as seen in the SEM images. The treatment also improved fabric comfort properties, the cotton substrates became less air permissive and more hygroscopic after the treatment. The experimental results indicated that propolis, beeswax and chitosan can be applied as an emulsion to functionalize cotton textile materials. The antibacterial performance of the functionalized fabrics suggested that the cotton fabrics treated with those biologically active natural compounds have the potentials to be used in medical fields. 相似文献
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Danko J Ondrasovic M Svický E Jenca A Pospieszny N Ondrasovicová O 《Anatomia, histologia, embryologia》2003,32(4):233-235
Acetylcholinesterase (ACHE)-positive nerve fibres in the thymus of the rat form perivascular plexuses as well as free ACHE-positive nerves, which do not follow the course of the vessels. A higher number of ACHE-positive nerves was observed in corticomedullary border and the medulla, a lower number in the cortex of the thymus lobuli. A gradual reduction of ACHE-positive intrathymus nerve formation was recorded and correlated with the increasing age of the rats. Nicotinamide adenine dinucleotide phosphate-diaphorase positive cells observed in the rat thymus were irregular with numerous projections and were located on the boundary of the cortex and medulla. 相似文献
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João M. Jesus Anthony S. Danko António Fiúza Maria-Teresa Borges 《Water, air, and soil pollution》2018,229(8):274
Salt-affected soils cover a wide area, limiting agricultural production worldwide. Several remediation options are available and include chemical and vegetative remediation, but several aspects of each process are not yet fully understood. Therefore, the goal of this work was to study the application of both techniques in a highly saline scenario and provide insights into the limits of the application of this technology. Two chemical amendments (CaSO4 and CaCl2) and two plant species (Juncus maritimus Lam. and Spartina maritima (Curtis) Fernald) were tested to remediate a non-calcareous soil with an electrical conductivity of 20 dS m?1 (EC) and a sodium adsorption ratio (SAR) of 45. Vegetative bioremediation experiments were performed under non-leaching conditions. As such, salts were redistributed and increased at the surface and decreased in depth due to capillary rise. In such conditions, there was no clear positive effect of plants on soil parameters. However, tested plants grew, accumulated, and excreted salts and sodium comparably to other research in the literature. Regardless, the obtained results suggest that plant salt uptake alone may not be sufficient for soil remediation, and therefore, other mechanisms may also play a significant role. As to chemical amendments, both chemicals used proved to be effective and reduced non-calcareous saline soil parameters to below threshold values of 4 dS m?1 for EC and 7 for SAR. However, CaCl2 was more effective and faster to remediate than CaSO4, likely due to higher solubility. Therefore, CaCl2 may be a viable, yet less tested, option for faster remediation processes. 相似文献
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Ribeiro Hugo da Silva Joana Gomes Jesus João Magalhães Catarina Dias Joana M. Danko Anthony S. 《Journal of Soils and Sediments》2019,19(1):439-450
Purpose
Biodiesel is a renewable fuel that can be mixed with toluene and be accidentally released into anoxic ecosystems and impact soil microbial communities. Therefore, the aim of the present work was to examine, under nitrate-reduction conditions, the biodegradation of toluene in the presence of two different types of biodiesel (sunflower and rapeseed), and their impact on the bacterial community structure.
Materials and methodsSediment samples were spiked individually with toluene, biodiesel, and their blends in laboratory-designed microcosms. Sunflower oil biodiesel was produced in the laboratory, while rapeseed oil biodiesel was a commercial product. Degradation of biodiesels and blends was monitored by directly measuring the substrate or indirectly by determining nitrate removal during the course of the experiment. Denitrification rates were estimated with the acetylene inhibition technique. Denaturing gradient gel electrophoresis was used to assess impacts on the bacterial community structure exposed to biodiesels, blends, and toluene.
Results and discussionThe results of this study showed that toluene and biodiesel were completely degraded within 10 days. Biodiesel significantly affected the bacterial community structure at a similar magnitude, independently of its origin. Additionally, toluene impacted the bacterial community and denitrification process to a lower extent than biodiesel and a clear decrease in the relative bacterial richness and diversity was shown in samples with biodiesel and blends. To the best of our knowledge, this is one of the first reports describing degradation of biodiesel alone and blends under nitrate-reducing conditions, and also the effects of these compounds on the denitrification process. In addition, due to the recently discovered “oxygenic denitrification” process, the acetylene inhibition technique and nitrous oxide quantification may not be the most adequate tool to estimate denitrification rates. Further detailed studies are advised to understand whether the identified bacterial community shift impacts ecosystem functions.
ConclusionsOur results help to understand the biodegradation of toluene, biodiesel, and their blends in sediments under nitrate-reducing conditions and might be important in implementing bioremediation strategies in anoxic environments.
相似文献9.
This study was aimed to compare structures of the thyroid tissue of transgenic rabbits expressing the human clotting factor VIII under the murine whey acidic protein promoter (mWAP-hFVIII rabbits) with the non-transgenic controls. Thyroid tissue samples were taken from transgenic and non-transgenic New Zealand White rabbits, examined by optical microscopy and analysed morphometrically. The analysis revealed no significant differences (P > 0.05) in the relative volume of basic thyroid structures. Furthermore, no significant differences (P > 0.05) were observed when measuring the epithelial height and nuclear diameter of the follicular cells. Altogether, this study demonstrates no negative effect of the mWAP-hFVIII transgenesis on the rabbit thyroid gland structure. 相似文献
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David Mazensky Jan Danko Eva Petrovova Slavka Flesarova Peter Supuka Anna Supukova Lenka Luptakova Halina Purzyc 《Animal Science Journal》2015,86(6):641-645
The aim of this study was to describe the arterial arrangement of the cervical spinal cord in the guinea pig. The study was carried out on 20 adult English self guinea pigs using corrosion and dissection technique. Batson's corrosion casting kit no. 17© was used as a casting medium. The origin of the ventral spinal artery from the left vertebral artery was found on average in 35% of the cases and from the right vertebral artery on average in 40% of the cases. The ventral spinal artery with origin from the anastomosis of two medial branches was found on average in 25% of the cases. The presence of ventral radicular branches of rami spinales entering the ventral spinal artery in the cervical region was observed in 42% of the cases on the right side and in 58% of the cases on the left side. The presence of dorsal radicular branches of rami spinales that reached the spinal cord was observed in 63% of the cases on the left side and in 37% of the cases on the right side. The number of radicular branches supplying the spinal cord is greater in guinea pig than in humans. 相似文献