Detection of Candida species by nested PCR method in one-humped camels (Camelus dromedarius)     

Parin  Ugur  Erbas  Goksel  Kirkan  Sukru  Savasan  Serap  Tugba Yuksel  H.  Balat  Gamze 《Tropical animal health and production》2018,50(2):421-425

Tropical Animal Health and Production - Systemic fungal diseases are the infections caused by false treatment protocols and generally are not taken into consideration especially in the veterinary...  相似文献   

Deficit irrigations during soybean reproductive stages and CROPGRO-soybean simulations under semi-arid climatic conditions     

Ergun Dogan  Halil Kirnak  Osman Copur 《Field Crops Research》2007

This study was carried out to determine the effect of water stress on five different generative stages of soybeans and to evaluate the CROPGRO-soybean model under semi-arid climatic conditions. The study was conducted at the Faculty of Agricultural Engineering, Harran University research field in 2003 and 2004 growing seasons. Plants received full irrigation during vegetative stages, after which it was cut off at different reproductive stages (treatments): R_1-2, beginning of flowering and full bloom; R₃, beginning of pod; R₄, full pod; R₅, beginning of seed; and R₆, full seed. The control treatment was full irrigation throughout. Observed yields ranged from 1955 (R₆) to 3684 kg ha⁻¹ (control) in 2003, and from 1867 (R₆) to 3952 kg ha⁻¹ (control) in 2004, respectively. Generally, in both of the years any water stress imposed on soybeans in three different generative stages (R₃, R₅, and R₆) resulted in substantial yield reduction compared with full irrigation; yield reduction was greatest at the R₆ stage. Biomass and 1000 seed weight also showed significant difference. Overall, CROPGRO-soybean simulated parameters from all treatments were higher compared with observed ones. Although simulated yield results were close to measured ones, they could not track observed yield patterns. Generally, the CROPGRO-soybean simulation model failed to satisfactorily mimic observed soybean yield, biomass, and 1000 seed weight and therefore it is suggested not to be used for similar scenarios and climatic conditions.  相似文献   

Mammary fibroadenoma in a lamb     

M. Yavuz Gulbahar  Tolga Guvenc  Murat Yarim  Yonca B. Kabak  Yuksel Sozgen 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):423-425

A fibroadenoma was diagnosed in the left udder of a 3-month-old female Chios lamb. No recurrence was observed after surgery. Grossly, the tumor had a whitish-gray lobular appearance, and the lobules were interlaced with thin septa. Microscopically, the tumor was composed of proliferating fibroepithelial tissue, including differentiated ducts lined by whorls and interlacing bundles of abundant loose fibrovascular stroma. Immunohistochemistry revealed the ductal epithelium to be positive for pancytokeratin (AE1/AE3) and loose fibrovascular stroma was positive for vimentin and basal cells covering the ductal epithelium of alpha-smooth-muscle actin. Immunostaining for the estrogen and progesterone receptors was negative. A diagnosis of mammary fibroadenoma was made based on the histological and immunohistochemical findings.  相似文献   

Hematological, biochemical, and behavioral responses of Oncorhynchus mykiss to dimethoate     

Dogan D  Can C 《Fish physiology and biochemistry》2011,37(4):951-958

The effects of dimethoate on hematological, biochemical parameters, and behavior were investigated in Oncorhynchus mykiss exposed to sublethal concentrations of 0.0735, 0.3675, and 0.7350 mg/l for 5, 15, and 30 days. Significant decrease was determined in erythrocyte and leukocyte counts, hemoglobin, hematocrit, MCV, and MCH, which was pronounced after prolonged exposure indicating the appearance of microcytic hypochromic anemia. There were no prominent changes in thrombocyte and MCHC. The glucose concentration showed an ascending pattern that proved to be positively correlated with duration. The protein concentration declined in higher dimethoate concentrations following 15 and 30 days. Negative and significant correlation was detected between glucose and protein concentrations. The fish showed remarkable behavioral abnormality such as loss of balance, erratic swimming, and convulsion. Present findings revealed that dimethoate exerts its toxic action even in sublethal concentrations and hematological parameters and abnormal behavior may be sensitive indicators to evaluate pesticide intoxication.  相似文献   

Textural properties and their correlation to cell structure in porous food materials     

Sozer N  Dogan H  Kokini JL 《Journal of agricultural and food chemistry》2011,59(5):1498-1507

This paper focuses on understanding the role of structural parameters and starch crystallization on the toughness of cake samples. Accurate mechanical measurements were performed to obtain toughness values, and these were related to structural parameters obtained from image analyses. Three-dimensional skeletons of food samples were generated by using X-ray tomography technique. The structural parameters (cell diameter, cell wall thickness, thickness to radius ratio (t/R), fragmentation index) were obtained after processing of the images with CTan software. The basic hypothesis of the paper is to show that the structural parameter t/R is a determinant for predicting toughness, which is a critical indicator of freshness. Freshness in cakes and other baked products is a leading factor in consumer perception. For this purpose three different cake formulations were stored at 37 and 50 °C. Cycling from these temperatures to lower storage temperatures of 25 and 4 °C was done to accelerate the starch retrogradation rate. Experimental results indicated that there was a strong interrelationship between morphological structure and the mechanical properties with regression coefficients of 0.68 and 0.95. Starch retrogradation, which was followed by X-ray diffractometry, was found to be directly proportional to toughness values, where the percent relative crystallinity increased with storage temperature.  相似文献   

Confirmation of reduced toxicity of deoxynivalenol in extrusion-processed corn grits by the MTT bioassay     

Cetin Y  Bullerman LB 《Journal of agricultural and food chemistry》2006,54(5):1949-1955

The objective of this study was to determine the loss of toxicity of deoxynivalenol in extruded cereal-based products by the tetrazolium salt (MTT) bioassay using a sensitive Chinese hamster ovary (CHO-K1) cell line and to compare the results to chemical (high-performance liquid chromatography, HPLC) and biochemical (enzyme-linked immunosorbant assay, ELISA) methods of analysis. A split-split plot design was used for the extrusion process experiments at temperatures of 150, 175, and 200 degrees C and screw speeds of 70 and 140 rpm. The initial mean deoxynivalenol concentration in the corn grits artificially contaminated with Fusarium graminearum was found to be 23.5 mug/g as measured by HPLC. The percent reductions of deoxynivalenol in the contaminated corn grits upon extrusion processing ranged from 22 to 35%, from 21 to 34%, and from 21 to 37% as measured by HPLC, ELISA, and MTT bioassay, respectively. The MTT bioassay results were more closely correlated with HPLC (r = 0.90) results than with ELISA results (r = 0.78). The MTT bioassay, using a sensitive mammalian cell line, was demonstrated to be a useful method for quantification of deoxynivalenol as well as a potential toxicity screening method for contaminated extruded cereal-based products.  相似文献   

Properties of modified ethylene terpolymer/poly(lactic acid) blends based films     

Sebnem Kemaloglu Dogan  Serap Gumus  Ayse Aytac  Guralp Ozkoc 《Fibers and Polymers》2013,14(9):1422-1431

In order to explore an alternative method instead of plasticization for improving the toughness, flexibility and processability of PLA based packaging films, two different kinds of modified polyethylene based elastomers, such as glycidyl methacrylate or maleic anhydride functionalized ethylene-acrylate based elastomers, were melt blended with PLA. Their properties were compared with conventional PEG plasticized PLA. The chemical interaction between end groups of PLA and epoxide or maleic anhydride functional groups of elastomers was shown by FTIR. Scanning electron microscopy showed that up to 20 % PEG loading, one phase morphology was achieved, however beyond this point, a phase separation was observed for plasticized PLA. For PLA/elastomer blends, a two-phase morphology was obtained as a result of immiscible nature of PLA and elastomers. Tensile and dynamic mechanical properties indicated that elastomer based blends were better than plasticized PLA independently from elastomer type. Differential scanning calorimeter (DSC) analysis exhibited that the T _g value was remarkably lowered in the plasticized PLA; however, it did not change in the case of elastomers. In terms of oxygen permeability and biodegradability, plasticized PLA was found to be better than elastomer based blends.  相似文献   

Association between Granulomatous Colitis in French Bulldogs and Invasive Escherichia coli and Response to Fluoroquinolone Antimicrobials     

A.C. Manchester  S. Hill  B. Sabatino  R. Armentano  M. Carroll  B. Kessler  M. Miller  B. Dogan  S.P. McDonough  K.W. Simpson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2013,27(1):56-61

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Remission of Histiocytic Ulcerative Colitis in Boxer Dogs Correlates with Eradication of Invasive Intramucosal Escherichia coli     

C.S. Mansfield  F.E. James  M. Craven  D.R. Davies  A.J. O'Hara  P.K. Nicholls  B. Dogan  S.P. MacDonough  K.W. Simpson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(5):964-969

Background: Historically, histiocytic ulcerative (HUC) (or granulomatous) colitis of Boxer dogs was considered an idiopathic immune-mediated disease with a poor prognosis. Recent reports of dramatic responses to enrofloxacin and the discovery of invasive Escherichia coli within the colonic mucosa of affected Boxer dogs support an infectious etiology.
Hypothesis: Invasive E. coli is associated with colonic inflammation in Boxer dogs with HUC, and eradication of intramucosal E. coli correlates with clinical and histologic remission.
Animals: Seven Boxer dogs with HUC.
Methods: Prospective case series. Colonic biopsies were obtained at initial evaluation in 7 dogs, and in 5 dogs after treatment with enrofloxacin. Biopsies were evaluated by standardized histopathology, and fluorescence in situ hybridization (FISH) with probes to eubacteria and E. coli .
Results: Intramucosal E. coli was present in colonic biopsies of 7/7 Boxers with HUC. Clinical response was noted in all dogs within 2 weeks of enrofloxacin (7±3.06 mg/kg q24 h, for 9.5±3.98 weeks) and was sustained in 6 dogs (median disease-free interval to date of 47 months, range 17–62). FISH was negative for E. coli in 4/5 dogs after enrofloxacin. E. coli resistant to enrofloxacin were present in the FISH-positive dog that relapsed.
Conclusions and Clinical Relevance: The correlation between clinical remission and the eradication of mucosally invasive E. coli during treatment with enrofloxacin supports the causal involvement of E. coli in the development of HUC in susceptible Boxer dogs. A poor response to enrofloxacin treatment might be due to colonization with enrofloxacin-resistant E. coli .  相似文献   

Immunohistochemical evaluation of inflammatory infiltrate in the skin and lung of lambs naturally infected with sheeppox virus     

Gulbahar MY  Davis WC  Yuksel H  Cabalar M 《Veterinary pathology》2006,43(1):67-75

The present study describes immunophenotypic characteristics of inflammatory infiltrate in the skin and lung of lambs naturally infected with sheeppox virus (SPV). Three lambs revealed typical cutaneous and pulmonary lesions of sheeppox. Histologically, cutaneous and pulmonary lesions consisted of hyperplastic and/or degenerative changes in the epithelium with mononuclear cells, neutrophils, and typical sheeppox cells (SPCs), which had a vacuolated nucleus and marginated chromatin with occasional granular intracytoplasmic inclusions. The inflammatory infiltrate in pox lesions in both skin and lung was characterized by the presence of MHC II+ dendritic cells, CD4+, CD8+, gammadelta+ T cells, IgM+ cells, and CD21+ cells. Loss of expression of MHC I and MHC II antigens was observed in the affected areas of skin and lung. SPCs, stained with anti-SPV antibody, were also positive for CD14 and CD172A, antigens expressed on monocytes and macrophages. CD14 and CD172A negative SPCs were considered to be SPV infected degenerated epithelial cells or fibroblasts.  相似文献