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Phylogenetic relationships within the suborder Trypanosomatina were inferred from the kinetoplast DNA minicircle conserved region sequences. Trees built using distance-matrix (Neighbor-Joining) and maximum parsimony methods showed that the minicircle conserved regions (CRs) provide a sensitive and specific molecular marker suitable for phylogenetic analyses of subspecies and strains of trypanosomatid flagellates, as testified by the subdivision of the genus Leishmania into the subgenera Leishmania. Viannia and Sauroleishmania. However, since Phytomonas and monogenetic parasites of insects represent the earliest diverging groups, the CRs do not seem to be useful for inference of relationships among major lineages of the order Kinetoplastida. 相似文献
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Pretzsch Hans Poschenrieder Werner Uhl Enno Brazaitis Gediminas Makrickiene Ekaterina Calama Rafael 《European Journal of Forest Research》2021,140(5):1295-1296
European Journal of Forest Research - 相似文献
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Valentin Podgorsky ;Emma Kovalenko ;Iryna Karpova ;Elena Sashchuk ;Ekaterina Getman 《农业科学与技术》2014,(6):541-546
The purpose of this work is to perform a detailed study of carbohydrate specificity of the new extracellular bacilli lectins which is considered to determine mechanisms of the lectins action. Sources of lectins were bacterial strains from Ukrainian collection of microorganisms. The optimized protocol of bacilli lectins isolation and purification included precipitation with ammonium sulfate with subsequent gel filtration chromatography on Sepharose CL-6B. Hemagglutinating activity of bacilli lectins and their fine carbohydrate specificity to sialic acids and their derivatives as well as sialic asid-containing and asialic glycoconjugates were studied. The ability of extracellular bacilli lectins to discriminate a- and 13-conformation of carbohydrate molecule and the type of connection between the monomers was determined. Studied lectins showed the most affinity to glycoconjugates containing both types of sialic acids （N-acetylneuraminic acid （Neu5Ac） and N-glycolylneuraminic acid （NeuGc）） and it is supposed to be a basis of their diagnostic and analytical potential. 相似文献
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Tatjana Gavrilenko Olga Antonova Anna Ovchinnikova Lubov Novikova Ekaterina Krylova Nina Mironenko Galina Pendinen Anna Islamshina Natalia Shvachko Stephan Kiru Ludmila Kostina Olga Afanasenko David Spooner 《Genetic Resources and Crop Evolution》2010,57(8):1151-1164
The germplasm collections of the Vavilov Institute of Plant Industry, Russia represent the first germplasm collection made for potatoes, now numbering 8,680 accessions. It has tremendous historical and practical importance and a rich history, having been used to document a polyploid series in the cultivated species, to formulate initial taxonomic hypotheses in potato, for studies of interspecific hybridization, and serving as the germplasm base for Russian breeding efforts. Despite its importance and size, there has never been a study of its molecular diversity, and there were many gaps in its passport data. The purpose of the present study is to obtain morphological, ploidy, and microsatellite (SSR) data needed to set up a useful subset of the collection of cultivated potatoes and closely related wild species, and to use this collection to study cultivated potato taxonomy and phylogeny. Through assessments of viability, passport data, and chromosome counts, we selected a subset of 238 cultivated and 54 wild accessions. A morphological and nuclear SSR study of these collections distinguished only three cultivated species: Solanum curtilobum, S. juzepczukii and S. tuberosum, not the many more cultivated potato species of prior taxonomic treatments. The SSR study supports the ideas of S. acaule as one of the parental species for S. curtilobum and S. juzepczukii. The morphological and SSR results are very similar to other recent studies of cultivated species, and show the need to reclassify the collection of cultivated potatoes by modern taxonomic criteria. 相似文献
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Voronina Liudmila P. Morachevskaya Ekaterina V. Akishina Maria M. Kozlova Oxana N. 《Journal of Soils and Sediments》2019,19(8):3226-3234
Journal of Soils and Sediments - The purpose of this paper is to evaluate the environmental state of the territory of the famous Russian landscape-architectural museum Kolomenskoye. This park... 相似文献
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Wen-Liang Chen Ekaterina Turlova Christopher L. F. Sun Ji-Sun Kim Sammen Huang Xiao Zhong Yong-Yuan Guan Guan-Lei Wang James T. Rutka Zhong-Ping Feng Hong-Shuo Sun 《Marine drugs》2015,13(4):2505-2525
Glioblastoma, the most common and aggressive type of brain tumors, has devastatingly proliferative and invasive characteristics. The need for finding a novel and specific drug target is urgent as the current approaches have limited therapeutic effects in treating glioblastoma. Xyloketal B is a marine compound obtained from mangrove fungus Xylaria sp. (No. 2508) from the South China Sea, and has displayed antioxidant activity and protective effects on endothelial and neuronal oxidative injuries. In this study, we used a glioblastoma U251 cell line to (1) explore the effects of xyloketal B on cell viability, proliferation, and migration; and (2) investigate the underlying molecular mechanisms and signaling pathways. MTT assay, colony formation, wound healing, western blot, and patch clamp techniques were employed. We found that xyloketal B reduced cell viability, proliferation, and migration of U251 cells. In addition, xyloketal B decreased p-Akt and p-ERK1/2 protein expressions. Furthermore, xyloketal B blocked TRPM7 currents in HEK-293 cells overexpressing TRPM7. These effects were confirmed by using a TRPM7 inhibitor, carvacrol, in a parallel experiment. Our findings indicate that TRPM7-regulated PI3K/Akt and MEK/ERK signaling is involved in anti-proliferation and migration effects of xyloketal B on U251 cells, providing in vitro evidence for the marine compound xyloketal B to be a potential drug for treating glioblastoma. 相似文献
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Comparative stability studies of poly(2-methyl-2-oxazoline) and poly(ethylene glycol) brush coatings
Pidhatika B Rodenstein M Chen Y Rakhmatullina E Mühlebach A Acikgöz C Textor M Konradi R 《Biointerphases》2012,7(1-4):1
Non-fouling surfaces that resist non-specific adsorption of proteins, bacteria, and higher organisms are of particular interest in diverse applications ranging from marine coatings to diagnostic devices and biomedical implants. Poly(ethylene glycol) (PEG) is the most frequently used polymer to impart surfaces with such non-fouling properties. Nevertheless, limitations in PEG stability have stimulated research on alternative polymers that are potentially more stable than PEG. Among them, we previously investigated poly(2-methyl-2-oxazoline) (PMOXA), a peptidomimetic polymer, and found that PMOXA shows excellent anti-fouling properties. Here, we compare the stability of films self-assembled from graft copolymers exposing a dense brush layer of PEG and PMOXA side chains, respectively, in physiological and oxidative media. Before media exposure both film types prevented the adsorption of full serum proteins to below the detection limit of optical waveguide in situ measurements. Before and after media exposure for up to 2 weeks, the total film thickness, chemical composition, and total adsorbed mass of the films were quantified using variable angle spectroscopic ellipsometry (VASE), X-ray photoelectron spectroscopy (XPS), and optical waveguide lightmode spectroscopy (OWLS), respectively. We found (i) that PMOXA graft copolymer films were significantly more stable than PEG graft copolymer films and kept their protein-repellent properties under all investigated conditions and (ii) that film degradation was due to side chain degradation rather than due to copolymer desorption. 相似文献
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The emerald ash borer, Agrilus planipennis , is a beetle native to East Asia where it is considered a minor pest, preferentially attacking weakened or dying ash trees. It was first discovered in North America in 2002 and has since become one of the most serious invasive insect pests, killing millions of healthy ash trees in urban and forested settings. Similar damage is now occurring in the region of Moscow, Russia, which causes serious concern for Europe. In this paper, we review the current knowledge on A. planipennis in Asia and North America, provide new information on its occurrence in the region of Moscow and Eastern Russia and make recommendations for research and management strategies in Europe. 相似文献
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Olesya I. Zhuravleva Alexandr S. Antonov Vo Thi Dieu Trang Mikhail V. Pivkin Yuliya V. Khudyakova Vladimir A. Denisenko Roman S. Popov Natalya Y. Kim Ekaterina A. Yurchenko Andrey V. Gerasimenko Anatoly A. Udovenko Gunhild von Amsberg Sergey A. Dyshlovoy Shamil S. Afiyatullov 《Marine drugs》2021,19(1)
Seven new deoxyisoaustamide derivatives (1–7) together with known compounds (8–10) were isolated from the coral-derived fungus Penicillium dimorphosporum KMM 4689. Their structures were established using spectroscopic methods, X-ray diffraction analysis and by comparison with related known compounds. The absolute configurations of some alkaloids were determined based on CD and NOESY data as well as biogenetic considerations. The cytotoxic and neuroprotective activities of some of the isolated compounds were examined and structure-activity relationships were pointed out. New deoxyisoaustamides 4–6 at concentration of 1 µM revealed a statistical increase of PQ(paraquat)-treated Neuro-2a cell viability by 30–39%. 相似文献