Fabrication of electrospun Poly L-lactide and Curcumin loaded Poly L-lactide nanofibers for drug delivery     

Elakkiya Thangaraju  Natarajan Thirupathur Srinivasan  Ramadhar Kumar  Praveen Kumar Sehgal  Sheeja Rajiv 《Fibers and Polymers》2012,13(7):823-830

The present work reports the preparation of Poly L-Lactide (PLLA) and Curcumin loaded Poly L-Lactide (CPLLA) nanofibers by electrospinning. A series of PLLA solution (12 wt %) and C-PLLA (12 wt % PLLA) solution containing Curcumin (0.5 wt % and 1 wt %)) were electrospun into nanofibers. SEM images showed the average diameter of PLLA and C-PLLA in the range of 50?C200 nm. The TEM images showed the dispersion of Curcumin on C-PLLA nanofibers. The XRD pattern indicated decreases of crystallinity with the increase in the amount of Curcumin. The characteristic peak of Curcumin was confirmed by FTIR. The TGA results showed the degradation of PLLA and C-PLLA close to 300 °C. The percentage porosity and the contact angle of PLLA were found to be 90.2 % and 115±3 ° with deionised water, respectively. The water uptake percentage was found to be 17.6 %. The percentage cumulative release of Curcumin at the end of 8th day for 0.5 and 1.0 wt % formulations was 81.4±1.3 and 86.7±1.7 % respectively. The in-vitro biological cytotoxicity studies were performed using C6 glioma cells and NIH 3T3 fibroblast by MTT assay and SEM analysis.  相似文献   

Method and quantity of liquid formulation of phosphobacteria required for seed inoculation     

Gomathy M  Thangaraju M  Gunasekaran S  Gopal NO  Gopal H 《Pakistan journal of biological sciences: PJBS》2008,11(1):86-91

The survival of vegetative and sporulated cells of the Bacillus cultures on the seeds of the crop plants was tried in different combinations. One milliliter inoculum with 1 mL adhesive combination or sterile water showed better results followed by 1.5 mL inoculum with 0.5 mL adhesive or sterile water. The population of 5.5x10(5) cfu seed(-1) on black gram, 10.5x10(5) cfu seed(-1) on soybean and 6.5x10(5) cfu seed(-1) on maize were observed after 12 h of incubation in 1 mL sporulated inoculum mixed with 1 mL of rice gruel. The sporulated inoculum along with rice gruel favoured the adherence of the regenerated cells as rice gruel is rich in nutrient content.  相似文献