Comparative mapping and discovery of segregation distortion and linkage disequilibrium across the known fragrance chromosomal regions in a rice F2 population     

Farahnaz Sadat Golestan Hashemi  Mohd Y. Rafii  Mohd Razi Ismail  Mahmud Tengku Muda Mohamed  Harun A. Rahim  Mohamad Abd Latif  Farzad Aslani 《Euphytica》2015,204(3):557-569

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Effect of Organic and Chemical Fertilizer on Soil Characteristics and Essential Oil Yield in Dragonhead     

Saeed Yousefzadeh  Mostafa Govahi  Ozra Sadat Khatamian Oskooie 《Journal of plant nutrition》2015,38(12):1862-1876

We conducted field experiments in two locations to investigation of organic and chemical fertilizer on soil characteristics and essential oil yield in dragonhead. Results showed that the inoculation of bacteria with the seeds in location 1 and also the inoculation of bacteria with the seeds of G₁ genotype in location 2 than G₂ genotype increased the total soil nitrogen content. The application of azocompost and its inoculation with urea's chemical fertilizer comparing to chemical control, it increase the soil carbon content. Results indicated that by increasing azocompost content with a linear procedure bulk density increased. Results showed that application of 100% azocompost treatment had the highest electrical conductivity in the soil. According to the results, the main effect of genotype and fertilizer regime and also two-way interaction (bacteria × fertilizer) had significant relationship with essential oil yield.  相似文献   

Comparative Evaluation of Conventional and Transvaginal Laparoscopic Ovariohysterectomy in Dogs     

Jalal Bakhtiari DVM  PhD  Ali Reza Khalaj MD  Elham Aminlou DVM  Amir Niasari‐Naslaji DVM  PhD 《Veterinary surgery : VS》2012,41(6):755-758

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Combined detection and introgression of quantitative trait loci underlying desirable traits     

Yazdi MH  Sonesson AK  Woolliams JA  Meuwissen TH 《Journal of animal science》2008,86(5):1089-1095

This study presents a new method that combines QTL mapping and gene introgression. The effectiveness of this method for simultaneous detection and introgression of a desirable QTL from a donor line into a recipient line was evaluated by simulation. For evaluation, we used the fourth backcross generation of 2 inbred lines. The difference between the 2 lines for the trait of interest was described entirely by 1 QTL, with the donor line carrying the superior allele. Nine scenarios, combinations of 3 heritabilities (h(2) = 0.10, 0.05, or 0.01) and 3 population sizes (N = 100, 500, or 1,000) were considered in the simulation. Selection of parents for the next backcross was based solely upon the estimated probability of carrying the superior allele after a QTL analysis. Estimates of the QTL location and allele substitution effect in most scenarios were comparable to the true values. However (with either small h(2) or N) the QTL allele substitution effect was underestimated, and location was also biased. The SE of the estimates decreased with increasing N. The retained donor chromosome segment and linkage drag were close to the expected values from other published work. In general, combined detection and introgression of genes underlying desirable traits not only saves at least 1 generation, but also it ensures that the desirable QTL is introgressed where its function is simultaneously tested in a planned environment and recipient genome structure.  相似文献   

Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4°C     

Abolfazl Ghaniei  Mohsen Eslami  Elham Zadeh Hashem  Reza Rezapour  Alireza Talebi 《Journal of animal physiology and animal nutrition》2019,103(3):713-722

Current study was carried out to examine the protective effects of quercetin against toxicity induced by hydrogen peroxide in rooster semen in vitro. Semen samples were collected from ten roosters (Ross 308 broiler breeder males, 32 weeks old) twice a week by abdominal massage method. Samples with ≥70% progressive motility were selected, pooled, diluted and used for the study. Experimental groups consisted of negative control, control that received solvent of quercetin, H₂O₂ (40 μM) and combination groups which incubated with constant dose of H₂O₂ (40 μM) plus various levels of quercetin (20, 40 and 80 μM). Measurement of total hydroperoxide (HPO), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC) and superoxide dismutase activity as well as routine sperm tests were done at 0, 24 and 48 hr of storage at 4°C. Results revealed that exposure to hydrogen peroxide significantly increased HPO (138.43 ± 7.32 vs. 66.08 ± 3.97 μmol/g protein), MDA (7.21 ± 0.08 vs. 5.71 ± 2.16 μmol/g protein) and NO (0.367 ± 0.013 vs. 0.215 ± 0.011 μmol/g protein) levels and decreased sperm progressive motility (27.28 ± 1.21 vs. 47.49 ± 1.29%), and amounts of TAC (11.49 ± 0.39 vs. 15.70 ± 0.79 mmol/g protein) compared to control at 24 hr (p < 0.05). Changes at mentioned variables were repeated at 48 hr of storage. Also, co‐administration of quercetin (especially at 40 and 80 μM) with hydrogen peroxide restored the toxic effects of hydrogen peroxide on rooster semen parameters such as primary and secondary lipid peroxidative indicators and other evaluated variables. The study concluded that rooster semen enrichment with quercetin would protect lipid peroxidative and nitrosative hydrogen peroxide‐mediated damage during cold liquid storage of rooster semen.  相似文献   

Population parameters for birth and ewe fleece weight at different parities in Baluchi sheep     

Yazdi MH  Näsholm A  Johansson K  Jorjani H  Liljedahl LE 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》1997,114(1-6):323-332

ZUSAMMENFASSUNG: Populationsparameter für Geburts- und Vlie?gewicht von Baluchi Schafen Das Datenmarterial stammt von zwei Herden einer Schafzuchtstation in NO Iran aus den Jahren 1966-1989. Die Tiere waren unselektiert und stammten aus zuf?llig verteilten Paarungen. Es wurden Geburtsgewicht und Vlie?gewicht bei verschiedenen Altersstufen erhoben und Varianzkomponenten mittels Restringierter Maximaler Likelihood mit einem bivariaten Tiermodell mit fixen Wirkungen von Jahr, Geschlecht, Geburtstyp und Parit?t sowie Zufallswirkungen für additiven Genotyp des Lammes (direkt) und des Mutterschafes (maternal), gemeinsamer Umwelt (ausgenommen Vlie?gewicht) und Resteinflu? gesch?tzt. Direkte und maternale genetische Korrelationen zwischen Leistungen verschiedener Parit?ten wurden berechnet. In Herde 1 scheinen Varianzen und Heritabilit?tswerte für Lammgewicht bis Parit?t 5 zuzunehmen, kaum aber in Herde 2. Die durchschnittlichen Heritabilit?tswerte, direkt, maternal und gesamt waren 0.12, 0.11 und 0.26, die genetische Korrelation zwischen direkten und maternalen Wirkungen 0.42. Bei Vlie?gewicht waren in Herde 1 keine Ver?nderungen der Varianzen und Heritabilit?tswerte mit Alter zu erkennen, aber bei Herde 2 nahmen ph?notypische und Umweltvarianz mit Alter leicht zu. Durchschnittliche direkte, maternale und Gesamtheritabilit?t waren 0.19, 0.04 und 0.22, die genetische Korrelation zwischen direkten und maternalen Wirkungen geringgradig positiv in Herde 1, aber mit Alter zunehmend negativ in Herde 2. Die genetischen Korrelationen für direkte Wirkungen auf Geburtsgewicht waren hoch zwischen Parit?ten 1 bis 5, aber niedriger bei Parit?t 6 und jene zwischen maternal bedingten Wirkungen zeigten ?hnliche Trends. In Herde 2 waren Werte mit Parit?t 6 ?hnlich wie die zwischen den übrigen Parit?ten. Die die Vlie?gewichte betreffenden direkt genetischen Korrelationen zwischen Parit?ten waren in beiden Herden ?hnlich (0.73-0.92), jene, die maternale Wirkungen betreffen, deutlich geringer, besonders soweit sie Parit?ten 5 und 6 betroffen haben und zeigten besonders bei Herde 2 starke Schwankungen (-0.54 bis 0.74). SUMMARY: Direct and maternal performance of ewes at different parities were examined in Baluchi sheep. The data set was collected during the period 1966-1989 from two flocks at a sheep breeding station in the north-east of Iran. The animals included in the data set were unselected and randomly mated. The traits analysed were birth weight of lamb and fleece weight at different parities of the ewe. Variance components were estimated using Restricted Maximum Likelihood with a bivariate animal model including fixed effects of year, sex, type of birth and parity, and random effects of additive genotype of lamb (direct genetic effect), additive genotype of ewe (maternal genetic effect) and common environment (excluded for ewe fleece weight), and random residual effect. Direct and maternal genetic correlations between different parities were estimated. There was evidence of increasing phenotypic and genetic variances and heritabilities from parity 5 for birth weight of lamb in flock 1, but only evidence of a slightly increasing age trend for the environmental and phenotypic variance in flock 2. The average heritabilities over flocks and parities were 0.12, 0.11 and 0.26 for the direct, maternal and total heritability, respectively, while the average genetic correlation between direct and maternal effects for this trait was 0.42. There were no indications of any age changes in variances or heritabilities for ewe fleece weight in flock 1, but indications of slightly increasing age trends for the environmental and phenotypic variance. The average heritabilities over flocks and parities were 0.19, 0.04 and 0.22 for the direct, maternal and total heritability, respectively, while the average genetic correlation between direct and maternal effects was slightly positive in flock 1 but increasingly negative with age of the ewe in flock 2. Direct genetic correlations between parities 1-5 were very high for birth weight of lambs (on average 0.96) in contrast to the markedly lower correlations of parities 1-5 with parity 6 (on average 0.67) in flock 1 with a similar pattern for the maternal genetic correlations. In flock 2, these correlations were also high but without the marked decrease between parities 1-5 with parity 6 that was found in flock 1. Direct genetic correlations between the various parities for ewe fleece weight were similar for the two flocks, ranging from 0.73 to 0.92 and without any obvious differences between the various combinations of parities. However, the maternal were markedly lower than the direct genetic correlations, especially for the combinations of parity 5 and 6 with the earlier parities, and most pronounced in flock 2 fluctuating from -0.54 to 0.79. To obtain reliable estimates of breeding values for birth weight of lamb, it is recommended that the prediction should include not only earlier but also later parities (ages) of the ewe.  相似文献   

Production of an Antibody Fragment (scFv) Targeting PcrV Protein of Pseudomonas aeruginosa in Fed-Batch Cultivation Mode     

Saba Karam  Mozhgan Raigani  Sahar Hassani Afshar  Yeganeh Talebkhan  Elham Bayat  Samira Komijani  Leila Nematollahi  Farzaneh Barkhordari  Mehdi Shafiee Ardestani  Fatemeh Davami 《Iranian Biomedical Journal》2021,25(6):390

Background: Pseudomonas aeruginosa is one of the opportunistic pathogens causing frequent hospital-acquired life-threatening infections in mechanically ventilated patients. The most significant virulence factor of P. aeruginosa is T3SS. PcrV is an important structural protein of the T3SS. Methods:In the current investigation, a recombinant scFv mAb against the PcrV protein was expressed in EnBase® (fed-batch) cultivation mode. The pETiteTM N-His SUMO Kan vector, including anti-PcrV scFv gene, was transformed into Escherichia coli (BL21) cells. The expression and solubility of anti-PcrV scFv protein were investigated at two different temperatures (25 °C and 30 °C) and at different induction times (4, 6, 8, 12, and 24 hours). Results:Increased efficiency was achieved by EnBase® compared to LB broth; owing to the slow release of glucose, the maximum level of solubility and total protein expression was observed in EnBase® cultivation system at 30 °C and 24 h post induction. Furthermore, IC₅₀ for anti-PcrV scFv protein was determined to be approximately 7 μg/mL. Conclusion:Anti-PcrV scFv produced in this study showed promising in vitro results, protecting RBC from lysis by P. aeruginosa (exoU+). Key Words: Fed Batch, recombinant protein, Pseudomonas aeruginosa, scFv  相似文献   

Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Günther, 1874)     

Sayyed Mohammad Hadi Alavi  Elham Jorfi  Azadeh Hatef  Sayyed Abdul Saheb Mortezavi 《Aquaculture Research》2010,41(10):e688-e694

Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in Barbus sharpeyi (Cyprinidae, Teleosotei). Spermatozoa concentration ranged from 9.77 to 20.20 × 10⁹ spermatozoa mL^?1. Osmolality (mOsmol kg^?1) and ionic contents (mM L^?1) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na⁺, 28.8±0.9 K⁺, 101.7±3.1 Cl^?, 0.9±0.1 Mg²⁺ and 2.1±0.1 Ca²⁺ respectively. Total protein and glucose were 5.3±0.2 g L^?1 and 76.7±4.3 mM L^?1 respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.  相似文献   

Mucoadhesive Chitosan Electrospun Nanofibers Containing Tetracycline and Triamcinolone as a Drug Delivery System     

Leila Behbood  Soroush Karimi  Esmaeil Mirzaei  Ghobad Mohammadi  Mahsa Azami  Elham Arkan 《Fibers and Polymers》2018,19(7):1454-1462

The mucoadhesive Chitosan (CS) nanofibers as a drug delivery system were developed. Chitosan was modified via the immobilization of thiol groups from L-cysteine as a mucoadhesive reagent. The mucoadhesive properties of the chitosan nanofibers were evaluated by tensiometer set and via tensile studies. Drug and mucoadhesive agent loading lead to decrease diameters and increased porous of nanofibers. The release of Tetracycline (Tet) and Triamcinolone (Tri) were increased with increasing immersion time and it became constant at long immersion times. Mucoadhesion studies were done at pH 2–7 and in pH 6 maximum mucoadhesive properties observed. Release studies demonstrated a sustained release of both drug continued up to 48 hours. Microbial studies were performed on the nanofibers. The drug delivery system represented a novel tool for improve the therapeutic efficacy of various drugs that are poorly absorbed from the gastrointestinal tract. Also it is an efficient system for treatment of oral ulceration.  相似文献   

Response of European seabass (<Emphasis Type="Italic">Dicentrarchus labrax</Emphasis>) to canola oil diets: effect on growth performance,fish health and liver and intestine histomorphology     

Elham A. Wassef  Olfat M. Wahbi  Eman M. Saqr  Norhan E. Saleh 《Aquaculture International》2016,24(4):1073-1088

This study was undertaken to assess the effects of feeding European seabass (Dicentrarchus labrax) canola oil-added diets on growth, health status and liver and intestine histomorphology. Seabass (56.18 ± 0.16 g initial body weight) were fed one of three fish meal-based diets with ~48 % crude protein and ~16.0 % lipid, combining fish oil (FO) and canola oil (CO) for 12 weeks. The diets contained: zero (control, CTRL), 45 (CO50) or 63 (CO70) g CO kg^?1 assigned in triplicates to three dietary groups. The results indicated that neither dietary oil type (FO or CO) nor CO level adversely affected (P > 0.05) the growth, feed utilization or major blood constituents’ composition as an indicator of the overall health status of fish. Despite the CO diets influence on head kidney macrophage activity being unappreciable (P > 0.05), there was a reducing trend with an increase in CO level incorporation. The CO70 diet induced a minor fat infiltration in hepatocytes and leucocytes infiltration, hyperplasia of the basal nuclei and supranuclear vacuolization of the enterocytes of the distal intestine. The present observations suggest that it is possible to incorporate up to 63 g CO Kg^?1 in the feed for European seabass juveniles without major negative effects on growth, health status or liver and intestinal histomorphology.  相似文献