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Rumi Kaida Tomomi Kaku Kei’ichi Baba Masafumi Oyadomari Takashi Watanabe Sri Hartati Enny Sudarmonowati Takahisa Hayashi 《Journal of Wood Science》2009,55(5):381-386
We examined the saccharification and fermentation of meals from Acacia mangium wood, Paraserianthes falcataria wood, and Elaeis guineensis trunk. The levels of enzymatic hydrolysis of cellulose and ethanol production were highest for P. falcataria wood and lowest for A. mangium wood. Ultrasonication pretreatment of meal further increased the rates of hydrolysis and ethanol production in meal from
P. falcataria wood. Through this pretreatment, hemicelluloses (xylan and xyloglucan) and cellulose were released in the meal from P. falcataria wood. Loosening of hemicellulose associations can be expected to make P. falcataria wood more useful for bioethanol production. 相似文献
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Rumi?KaidaEmail author Tomomi?Kaku Kei’ichi?Baba Sri?Hartati Enny?Sudarmonowati Takahisa?Hayashi 《Journal of Wood Science》2009,55(6):435-440
Lignocellulosic material from trees has great potential to form the basis of the second generation for bioethanol production
because trees produce most of the biomass on the earth. We modified the wall structure of sengon (Paraserianthes falcataria) through overexpression of poplar cellulase in the cell walls. The overexpression did not decrease cellulose content but
caused a decrease in xyloglucan bound to the walls. The level of saccharification and successive ethanol production was increased
in the wood of the transgenic sengon overexpressing poplar cellulase compared with that of the wild type plant, and even after
delignification of the wood. We propose that a xyloglucan intercalated into cellulose microfibrils could be one of the recalcitrant
components in the saccharification of lignocelluloses. 相似文献
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Background:Levan or fructan, a polysaccharide of fructose, is widely used in various commercial industries. Levan could be produced by many organisms, including plants and bacteria. The cloning of the gene from Bacillus licheniformis, which expressed levansucrase in Escherichia coli host, was carried out successfully. In the present study, we performed the in vitro production of levan and analyzed its potential application as antibacterial and antioxidant agents. Methods: In vitro levan production catalyzed by heterologous-expressed levansucrase Lsbl-bk1 and Lsbl-bk2 was optimized with BW design. The antibacterial activity of the produced levan was carried out using agar well diffusion method, while its antioxidant activity was tested by free radical scavenging assays. Results:The optimum conditions for levan production were observed at 36 °C and pH 7 in 12% (w/v) sucrose for levansucrase Lsbl-bk1, while the optimum catalysis of levansucrase Lsbl-bk2 was obtained at 32 oC and pH 8 in the same sucrose concentration. The in vitro synthesized levan showed an antibacterial activity within a concentration range of 10-20% (w/v) against Staphylococcus aureus, E. coli, and Pseudomonas aeruginosa. The same levan was also able to inhibit the DPPH radical scavenging activity with the antioxidant strength of 75% compared to ascorbic acid inhibition. Conclusion:Our study, therefore, shows that the optimized heterologous expression of levansucrases encoded by Lsbl-bk1 and Lsbl-bk2 could open the way for industrial levan production as an antibacterial and antioxidant agent. Key Words: Antioxidants, Fructans, In vitro technique, Levan 相似文献
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Tomomi Kaku Rumi Kaida Kei��ichi Baba Sri Hartati Enny Sudarmonowati Takahisa Hayashi 《Journal of Wood Science》2011,57(6):545-548
Recalcitrance to saccharifi cation is a major limiting factor of the conversion of lignocellulosic biomass to ethanol. Levels of wood saccharification and subsequent ethanol production were higher in transgenic mangium (Acacia mangium) trees overexpressing xyloglucanase than in wild-type plants, even after delignification of the wood. We propose that a decrease in the quantity of xyloglucan that is intercalated into cellulose microfibrils could facilitate the process of saccharification. 相似文献
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Sri Hartati Enny Sudarmonowati Tomomi Kaku Hisato Ikegaya Rumi Kaida Kei’ichi Baba Takahisa Hayashi 《Journal of Wood Science》2011,57(6):463-469
Transgenic mangium trees (Acacia mangium) overexpressing xyloglucanase (AaXEG2) were generated by spraying flower buds with Agrobacterium solution and allowing seeds to develop. The overexpression of xyloglucanase decreased xyloglucan content in the cell walls
and increased stem length and diameter. The leaves of the transgenic seedlings exhibited accelerated heteroblastic development,
proceeding from the stage of three bipinnate leaves to that of enlarging petiole 2 weeks earlier than wild type seedlings
did. 相似文献
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