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Takeda Y Asaoka H Ueno M Jimma F Hidaka M Shibusawa H Kaneda K Saniabadi AR Hiraishi K Kashiwagi N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):841-842
Assessment of spleen size using the ultrasonography has become a standard practice in human. However, the assessment is not established method in experimental animals. To establish the index to assess the spleen size using ultrasonography, we measured the cross-section image of rabbit spleen during endotoxin shock. The image of the cross-section was appeared as triangle, and the height of the triangular image was defined as the spleen index. This spleen index showed strong correlation with the spleen weight. In conclusion, this method is suitable for observation of changes in rabbit spleen size and may reduce the number of rabbit in the longitudinal studies. 相似文献
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Molecular investigation of 16 strains, conventionally identified to be Malassezia pachydermatis, isolated from dogs in Japan was carried out by random amplification of polymorphic DNA (RAPD) and chitin synthase 2 (CHS2) gene sequence analyses. The RAPD band patterns of 13 clinical isolates were identical to that of standard strain of M. pachydermatis (CBS-1879). The other three clinical isolates were different from the standard strain of M. pachydermatis in RAPD patterns, and two of the three isolates were identical. About 620 bp genomic DNA fragments of the CHS2 gene were amplified from the same 16 clinical isolates of M. pachydermatis by polymerase chain reaction (PCR) and sequenced. The phylogenetic analysis of the nucleotide sequences of CHS2 gene fragments of the 16 clinical isolates revealed that the 13 strains were genetically very close to the standard strain of M. pachydermatis and the other two isolates were genetically close to the standard strain of M. furfur rather than M. pachydermatis. The remaining one isolate was phylogenetically distinct from all the seven Malassezia species reported so far. 相似文献
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Fumie MAGATA Yoshikazu ISHIDA Akio MIYAMOTO Hidefumi FURUOKA Hisashi INOKUMA Takashi SHIMIZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):81-84
We investigated the
concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian
follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old
lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after
normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the
vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid
were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven
follicles showed an extremely high level of LPS (12.40 EU/ml) compared to
the other follicles (0.62–0.97 EU/ml). These results might suggest the
presence of high concentration of LPS in follicles in cows with postpartum metritis. 相似文献
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Nitrogen-fixing activity in decomposing litter of three tree species at a watershed in eastern Japan
Takashi Yamanaka Keizo Hirai Shuhei Aizawa Shuichiro Yoshinaga Masamichi Takahashi 《Journal of Forest Research》2011,16(1):1-7
Nitrogen fixation during litter decomposition was studied for 34 months using litterbags containing newly fallen litter of
coniferous species Cryptomeria japonica and Pinus densiflora and that of deciduous species Quercus serrata. Litterbags were set in contact with the forest floor in a deciduous broad-leaved forest near the top of a slope and in a
C. japonica stand at the middle of the slope at a watershed in eastern Japan. Nitrogen-fixing activity, estimated by acetylene reduction
after 16 and 19 months of incubation, was 62.65–3.86 nmoles C2H4 h−1 g−1 DW in Cryptomeria litter, but only 1.07–0.09 in Pinus and 0.72–0.04 in Quercus. The rate of N increase in decomposing litter was highest in Cryptomeria. Fungal biomass in decomposing litter, estimated by ergosterol content, increased during the initial 16 months of incubation
in Cryptomeria and Quercus, and during the initial 19 months of incubation in Pinus. The litter decomposition rate was highest in Cryptomeria among the three species, due to increased N content and fungal biomass in Cryptomeria litter. Thus, N increase in decomposing Cryptomeria litter affects the subsequent N dynamics and decomposition pattern. 相似文献
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Rohny Setiawan Maail Kenji Umemura Hideo Aizawa Shuichi Kawai 《Journal of Wood Science》2011,57(4):302-307
This study examined the effects of supercritical CO2 treatment on the curing and degradation of cementbonded particleboard (CBP). Significant correlations were found between
the supercritical CO2 treatment and mechanical properties during both curing and degradation processes. Internal bond (IB) strength, modulus of
rupture (MOR), and modulus of elasticity (MOE) values of CBP achieved their maximums by supercritical CO2 treatment in 30 min. These conditions indicated that supercritical CO2 treatment accelerates the curing process rapidly and enhances the mechanical properties of the CBP. However, these values
decreased in treatment from 60 min to 10 days and had a negative effect on board performance, indicating that supercritical
CO2 treatment over a longer time span leads to degradation of the CBP. Furthermore, X-ray diffractometry (XRD), thermal gravimetry
(TG-DTG), and scanning electron microscopy (SEM) observation clarified that the mechanisms of degradation are directly affected
by the mineralogical composition of the system, in par ticular, by the calcium carbonate content as caused by carbonation. 相似文献
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Zheng Z Narita M Takahashi M Liu A Furukawa T Toba K Aizawa Y 《Comparative immunology, microbiology and infectious diseases》2004,27(2):93-103
In order to apply for reducing graft versus host disease in allogeneic stem cell transplantation, the study concerning the induction of specific T cell anergy was designed. Normal allogeneic lymphocytes, which were co-cultured with IL-10-treated immature dendritic cells in the first mixed leukocyte culture (MLC), were cultured with mature dendritic cells of the same origin as IL-10-treated immature dendritic cells in the second MLC. By co-culturing with IL-10-treated immature dendritic cells, the response of normal lymphocytes to mature dendritic cells cultured from the same individual as that of IL-10-treated dendritic cells was markedly reduced, compared with the lymphocytes cultured with non-treated dendritic cells or IL-10-treated dendritic cells from a third party individual. The present study demonstrated that antigen specific T cell anergy was generated by priming allogeneic lymphocytes with IL-10-treated immature dendritic cells. These data suggested the applicability of IL-10-treated recipient dendritic cells for the induction of recipient cell-specific donor T cell anergy in donor graft. 相似文献
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Kazuya Iizuka Narumi Toya Jyunichi Ohshima Futoshi Ishiguri Naoko Miyamoto Mineaki Aizawa Tatsuhiro Ohkubo Chisato Takenaka Shinso Yokota 《Journal of Wood Science》2018,64(1):59-64
To utilize forest resources in areas affected by fallout from the Fukushima Daiichi Nuclear Power Plant accident, it is important to understand the mechanisms of 137Cs movement through the stem wood of contaminated trees. Understanding the mechanism of absorption and migration of 137Cs to stem wood is necessary for clues to the future prediction of the transition of 137Cs to xylem. In the present study, radial variations in 137Cs concentration were investigated in Japanese cedar (Cryptomeria japonica D. Don) trees collected 1 year and 10 months after the accident. Additionally, the relationship between 137Cs concentration and potassium (K) content was established. Trees with a higher moisture content and lower lightness value in heartwood tended to have a higher 137Cs concentration in the heartwood. In these trees, 137Cs concentration peaked at the heartwood–sapwood boundary and gradually decreased toward the pith. By contrast, K content within the heartwood remained nearly constant along the radial direction. The heartwood-to-sapwood ratio of 137Cs concentration was significantly positively correlated with that of K content. Based on these results, we suggest that 137Cs movement from sapwood to heartwood might be related to the K content ratio of heartwood and sapwood. 相似文献