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1.
Azari O Babaei H Derakhshanfar A Nematollahi-Mahani SN Poursahebi R Moshrefi M 《Veterinary research communications》2011,35(4):211-222
The aim of this study was to investigate the effects of transplanted Wharton’s jelly mesenchymal stem cells (WJMSCs) of caprine
umbilical cord on cutaneous wound healing process in goat. After collection of caprine pregnant uterus of mixed breed goats
from abattoir, the Wharton’s jelly (WJ) of umbilical cord was harvested. The tissues were minced in ventilated flasks and
explant culture method was used for separating mesenchymal stem cells (MSCs). The isolated cells were immunostained for Actin
protein, histochemically assayed for the presence of alkaline phosphatase activity, and analyzed for detection of matrix receptors
(CD44) and hematopoetic lineage markers (CD34), using flow cytometery. After The isolated cells, 3 × 106 MSCs were stained with BrdU and prepared for transplantation to each wound. Four 3-cm linear full thickness skin incisions
were made on both sides of thoracic vertebrate of four Raeini goats (two wounds on each side). The left wounds were implanted
with MSCs in 0.6 ml of Phosphate buffer saline (PBS), and the right wounds considered as control group that received 0.6 ml
of PBS. The samples were taken from the wounds 7 and 12 days after the wounding, and healing process was compared histologically
between the two groups. Anti-BrdU staining showed that the transplanted cells were still alive in the wound bed during the
study. The histopathological study revealed that re-epithelialization was complete at days 7 in treated wounds with WJMSCs,
whereas in control wound the wounds still showed incomplete epithelialization 12 days after wounding. Also, microscopic evaluation
showed less inflammation, thinner granulation tissue formation with minimum scar in the treated wounds in comparison with
control wounds. In conclusion, this study demonstrates the beneficial effect of caprine WJMSCs in cutaneous wound healing
in goat. 相似文献
2.
1. The objective of the study was to investigate the polymorphisms in two regions of the calpain 1 (CAPN1) gene and their association with breast and thigh meat quality in Japanese quail (ultimate pH (pHu), lightness, redness, yellowness, drip loss, thawing-cooking loss, water holding capacity and shear force, SF).
2. Blood samples were collected randomly from 100 birds and DNA was extracted using a commercial kit. Genotypes were determined by PCR amplification followed by single-strand conformation polymorphism (SSCP) analysis. The effect of CAPN1 genotypes on meat quality traits were analysed using a general linear model (GLM) procedure.
3. Genotypes of the CAPN1 gene in the first region (217-bp) analysed were significantly associated with yellowness and SF. The TT genotype showed significantly higher yellowness and lower shear force (more tenderness) than CT and CC genotypes. Genotypes of the second region of the gene (intron 4, 800-bp) were significantly associated with pHu, redness and SF of the breast meat. The BB genotype showed significantly lower pHu and redness and higher SF (lower tenderness) than other genotypes.
4. Information on polymorphisms of the CAPN1 gene will eventually provide useful information for improving meat quality of Japanese quail through marker-assisted selection. 相似文献
3.
Mehdi Azari Mojtaba Kafi Bita Ebrahimi Roya Fatehi Mahboobeh Jamalzadeh 《Veterinary research communications》2017,41(1):49-56
Objective
To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification.Methods
Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: non-vitrified COCs (control), vitrification 1 group (V1); vitrification was performed by 15% ethylene glycol (EG) and 15% DMSO in holding media (TCM-199 with 20% FCS); and vitrification 2 group (V2); vitrification was performed by 40% EG in holding media. After vitrification, COCs were warmed in two steps and cultured and then evaluated for nuclear maturation, embryo development and gene expressions.Results
The mean (±SD) percentages of nuclear maturation and blastocyst/cleaved were higher in control group (79.5 ± 8.0 and 31.0 ± 5.1%) than the V1 (34.8 ± 9.1 and 4.4 ± 5.1%) and V2 (47.8 ± 11.7 and 7.1 ± 5.8%) groups (P < 0.05), respectively. Further, COCs in V2 group showed higher mean (±SD) percentages of cleavage compared to V1 group (31.8 ± 1.0 vs 21.7 ± 2.8%; P < 0.05). GDF9 and BMP15 expression levels were higher in COCs in the control than of the vitrification groups (P < 0.05). In addition, expression level of GDF9 and BMP15 was higher in V2 group than in V1group (P < 0.05). The expression of HAS2 and FGF17 in V1 group was lower (P < 0.05) than that of the V2 groups.Conclusions
Expression of oocyte maturation genes was affected by vitrification procedure and conditions. Using EG alone for vitrification of bovine immature COCs, resulted in higher expression of GDF9, BMP15 and production of more in vitro matured and cleaved oocytes.4.
Ahani Azari Ania Amanollahi Reza Jafari Jozani Razi Trott Darren J. Hemmatzadeh Farhid 《Tropical animal health and production》2020,52(3):1043-1047
Tropical Animal Health and Production - Mycoplasma species cause wide ranges of infectious diseases in human and animals. The aim of the present study was to evaluate a real-time polymerase chain... 相似文献
5.
Azari O Molaei MM Emadi L Sakhaee E Esmaeili M 《Veterinary anaesthesia and analgesia》2012,39(3):291-295
ObjectiveTo evaluate the clinical and physiological effects of epidural injection of ketamine in camels.Study designRandomized prospective study.AnimalsTen healthy immature male dromedary camels.MethodsKetamine was administered epidurally at doses of 1 and 2 mg kg?1 (five animals in each treatment). The drug was injected into the first intercoccygeal epidural space. Anti-nociception, sedation, ataxia, and effect on cardiopulmonary, rectal temperature and some selected haematological parameters were recorded at different intervals before (baseline) and after the drug administration. Data were analyzed by anova or U Mann–Whitney tests, as relevant and significance was taken as p < 0.05.ResultsEpidural ketamine at the 2 mg kg?1 dose produced complete anti-nociception in the tail, anus and perineum, whilst the 1 mg kg?1 dose produced complete anti-nociception only in the tail. Epidural ketamine resulted in mild to moderate sedation at the 1 mg kg?1 dose and deep sedation at the 2 mg kg?1 dose. Ataxia was observed in all test subjects and was severe, resulting in recumbency, in the 2 mg kg?1 group. Respiratory rate and rectal temperature did not change significantly after injection of either treatment. Following epidural injection of 2 mg kg?1 of ketamine, heart rate increased significantly from the pre-injection baseline of 55 ± 2 to 76 ± 4 (mean ± SD) beats minute?1, but after the lower dose changes were not significant. The only significant changes in measured haematologic parameters were decreases in total erythrocyte count at 45 minutes and total leukocyte count from 45–75 minutes, in the 2 mg kg?1 group.ConclusionEpidural ketamine injection was associated with caudal anti-nociception, sedation and ataxia in the dromedary camels; the intensity and duration of which was dose dependant.Clinical relevanceNeither of the doses of epidural ketamine injection in our study was applicable for standing surgical procedures in dromedary camels. 相似文献
6.
Mohsen Pourasadi Bahram Falahatkar Ghobad Azari Takami 《Aquaculture International》2009,17(4):317-321
A minimally invasive surgical technique (MIST) for the removal of ovulated eggs from Persian sturgeon Acipenser persicus was tested on broodstocks caught from the wild to determine whether it affected fecundity or hatching rates compared with
the traditional stripping method of killing and removing the eggs (commonly used in hatchery). Morphological parameters of
females, germinal vesicle (GV) position, weight of obtained egg, number of eggs/gram, fertilization rate, and percentage of
hatching during incubation were not significantly different between the MIST and traditional stripping methods. Obtained ova
were 4.8 ± 0.4 kg female−1 in the MIST and 4.6 ± 0.5 kg female−1 in traditional stripped groups, respectively; the fertilization rate was 83.4 ± 11.2% and 80.0 ± 7.2% in groups, respectively.
The results of this study showed that the minimally invasive surgical technique approach is efficient, practical, and less
stressful to Persian sturgeon broodstocks during artificial propagation than other reported egg collection procedures. 相似文献
7.
Background:Inflammatory bone resorption in periodontitis can lead to tooth loss. Systemic administration of bisphosphonates such as risedronate for preventing bone resorption can cause adverse effects. ALG and PLGA microparticles have been studied as drug delivery systems for sustained release of drugs. Therefore, the release pattern of risedronate from PLGA microparticles embedded with ALG was studied as a drug delivery system for sustained release of the drug, which can be used in local administrations. Methods:Risedronate-containing PLGA microparticles were fabricated using double emulsion solvent evaporation technique. Ionic cross-linking method was used to fabricate risedronate-loaded ALG. Risedronate-containing PLGA microparticles were then coated with ALG. The calibration curve of risedronate was traced to measure EE and study the release pattern. SEM imaging was carried out, and cell toxicity was examined using MTT assay. Statistical analysis of data was carried out using SPSS ver. 20 software, via one-way ANOVA and Tukey’s tests. Results:SEM imaging showed open porosities on ALGs. The mean EE of PLGA microparticles for risedronate was 57.14 ± 3.70%. Risedronate released completely after 72 h from ALG, and the cumulative release was significantly higher (p = 0.000) compared to PLGA microspheres coated with ALG, which demonstrated sustained released of risedronate until day 28. Risedronate-loaded ALG showed a significant decrease in gingival fibroblasts cell viability (p < 0.05). Conclusion: Alginate-coated PLGA microspheres could release risedronate in a sustained and controlled way and also did not show cell toxicity. Therefore, they seem to be an appropriate system for risedronate delivery in local applications. Key Words: Alginates, Hydrogels, Polylactic acid-polyglycolic acid copolymer, Risedronic acid 相似文献
8.
Mohammad Ali Jalali Ehsan Ahmadifar Mohammad Sudagar & Ghobad Azari Takami 《Aquaculture Research》2009,40(7):804-809
Growth performance, carcass quality, survival and haematological responses were determined when Huso huso juvenile (41.7±1.8 g) fed diets containing Ergosan (an algal product) at 0, 2.0, 4.0 and 6.0 g kg−1 for 60 days. Each diet was fed to triplicate groups of fish at 10-day intervals (1–10, 20–30 and 40–50 with non-supplemented diets and 10–20, 30–40 and 50–60 with supplemented diets). Results showed that fish fed diets containing Ergosan had significantly higher growth than the control group ( P <0.05). Survival was not different among all dietary treatments ( P >0.05). Food conversion ratio in the fish fed a diet containing 4.0 and 6.0 g kg−1 Ergosan was significantly better than the other treatments ( P <0.05), whereas protein efficiency ratio was not different between experimental diets. Lymphocyte count in the fish fed diets containing Ergosan was higher than the other treatments. Haematocrit, haemoglobin (Hb) concentration, number of erythrocytes, total leucocytes, monocyte, eosinophil, myelocyte, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were not different between treatments. Neutrophil count in the control group was higher than the fish fed supplemented diets. Furthermore, whole body lipid, moisture and fibre were not different among dietary treatments ( P >0.05) but body protein in the fish fed a diet containing Ergosan at the level of 2.0 and 4.0 g kg−1 was higher than the other treatments. Whole body ash content was higher in the control group. It was concluded that dietary administration of Ergosan can influence some growth and haematological parameters in great sturgeon, H. huso juveniles. 相似文献
9.
Distribution and Contamination of Heavy Metal in the Coastal Sediments of Port Klang, Selangor, Malaysia 总被引：1，自引：0，他引：1
Seyedeh Belin Tavakoly Sany Aishah Salleh Majid Rezayi Naghmeh Saadati Leila Narimany Ghazaleh Monazami Tehrani 《Water, air, and soil pollution》2013,224(4):1-18
The coastal sediments of Port Klang in Malaysia were monitored from November 2009 to October 2010 to evaluate the spatial distribution of heavy metals As, Cu, Cd, Cr, Ni, Pb, Zn, Pb, Mn, Al and Fe. Sediment samples were collected from 21 stations, covering three subsidiary ports, namely West Port, North Port and South Port, at three-month intervals. The geo-accumulation index and the enrichment factor were estimated to assess contamination status based on background values. Additionally, the sediment quality guide was applied to evaluate the adverse biological effects of heavy metals. These results show that the concentrations of some metals, such as As, Cd and Pb were comparatively higher than the background values. This is considered a critical environmental issue for this region. Furthermore, the toxicity estimation indicates that living organisms have a high risk of As exposure. Spatial distribution maps of heavy metals would aid in the identification of pollution sources and vulnerable sites. 相似文献
10.
Summary Commercially grown fruit trees in the U.S. are almost invariably compound genetic systems with the top or scion cultivar budded or grafted to root systems derived from genetically unique zygotic seedlings. Although variation in tree size, yield, and cropping efficiency (CE) exists among trees within individual orchards, there have been no attempts to partition this variation in individual tree performance between the heterogeneity of zygotic seedling genotypes utilized as rootstocks and site-specific environmental variables such as soil type, fertility, and soil moisture, etc. We compared Field Blocking (FB), Moving Average (MA), and State Space (SS) procedures to infer environmental trends. Deviations from the estimated environmental effects that compose such trends were used to develop mass selection alternatives. FB was seen as a discrete approximation to an environmental response surface that varied regularly along tree rows. Regular trends within blocks for FB will result in a confounding of genetic and environmental effects and will decrease the precision of estimates of genetic potentiality based on block mean deviations. Trends estimated using the MA and SS procedures were very similar and appeared to minimize the confounding of genetic potentiality with the environmental response surface. Deviations from environmental trends estimated using the MA and SS procedures demonstrate that genetic variation of J. hindsii seedling rootstokes accounted for about 20% of the total variance in tree yield. Evidence for genotypic variation in CE was less clear. 相似文献