全文获取类型
| 收费全文 | 204篇 |
| 免费 | 10篇 |
| 国内免费 | 1篇 |
专业分类
| 林业 | 29篇 |
| 农学 | 7篇 |
| 基础科学 | 1篇 |
| 48篇 | |
| 综合类 | 17篇 |
| 农作物 | 22篇 |
| 水产渔业 | 12篇 |
| 畜牧兽医 | 50篇 |
| 园艺 | 4篇 |
| 植物保护 | 25篇 |
出版年
| 2023年 | 3篇 |
| 2022年 | 3篇 |
| 2021年 | 8篇 |
| 2020年 | 6篇 |
| 2019年 | 4篇 |
| 2018年 | 8篇 |
| 2017年 | 10篇 |
| 2016年 | 12篇 |
| 2015年 | 6篇 |
| 2014年 | 9篇 |
| 2013年 | 12篇 |
| 2012年 | 12篇 |
| 2011年 | 20篇 |
| 2010年 | 11篇 |
| 2009年 | 8篇 |
| 2008年 | 15篇 |
| 2007年 | 12篇 |
| 2006年 | 12篇 |
| 2005年 | 7篇 |
| 2004年 | 9篇 |
| 2003年 | 8篇 |
| 2002年 | 3篇 |
| 2001年 | 2篇 |
| 2000年 | 1篇 |
| 1999年 | 1篇 |
| 1998年 | 1篇 |
| 1997年 | 3篇 |
| 1996年 | 3篇 |
| 1994年 | 1篇 |
| 1988年 | 1篇 |
| 1978年 | 1篇 |
| 1977年 | 1篇 |
| 1976年 | 1篇 |
| 1975年 | 1篇 |
排序方式： 共有215条查询结果，搜索用时 31 毫秒
1.
2.
3.
Effect of farm characteristics and practices on hygienic quality of ovine raw milk used for artisan cheese production in central Italy 
下载免费PDF全文
下载免费PDF全文 Elisa Carloni Annalisa Petruzzelli Giulia Amagliani Giorgio Brandi Francesco Caverni Piermario Mangili Franco Tonucci 《Animal Science Journal》2016,87(4):591-599
A survey on ovine dairy farms directly transforming own‐produced milk, in the Italian Marche region, was carried out to assess flock and milking practices that may influence milk hygienic‐sanitary conditions. A census survey established that 24 dairy farms were located in this region. Bulk milk samples were collected throughout the milking period in each dairy farm in 2013. Analyzed variables were: (i) chemical parameters such as fat, protein and lactose content, dry matter and pH; and (ii) total bacterial (TBC) and somatic cell counts (SCC). Chemical parameter values were in agreement with published data while, geometric mean (GM) log10 SCC was 5.91 and TBC GM was 57 978 colony forming units/mL, in compliance with Eropean Union criteria. A positive correlation was found between SCC and TBC when GMs of all farm data were considered (Spearman's rho = 0.7925; P = 0.0001). Statistical analysis did not show significant correlation between SCC or TBC GM and dairy farm principal characteristics. Although SCC levels detected in the present study should suggest the need to implement mastitis control programs, Marche's dairy sheep flocks revealed a good hygienic condition level. This is an important aspect in implementing safety for end users of the final product. 相似文献
4.
5.
Carmela Gallo Giusi Barra Marisa Saponaro Emiliano Manzo Laura Fioretto Marcello Ziaco Genoveffa Nuzzo Giuliana d&#x;Ippolito Raffaele De Palma Angelo Fontana 《Marine drugs》2020,18(12)
Immunotherapy takes advantage of the immune system to prevent, control, and eliminate neoplastic cells. The research in the field has already led to major breakthroughs to treat cancer. In this work, we describe a platform that integrates in vitro bioassays to test the immune response and direct antitumor effects for the preclinical discovery of anticancer candidates. The platform relies on the use of dendritic cells that are professional antigen-presenting cells (APC) able to activate T cells and trigger a primary adaptive immune response. The experimental procedure is based on two phenotypic assays for the selection of chemical leads by both a panel of nine tumor cell lines and growth factor-dependent immature mouse dendritic cells (D1). The positive hits are then validated by a secondary test on human monocyte-derived dendritic cells (MoDCs). The aim of this approach is the selection of potential immunotherapeutic small molecules from natural extracts or chemical libraries. 相似文献
6.
In the trapping ofTribolium castaneum (Herbst), the activity of traps baited or not baited with aggregation pheromone 4,8-dimethyldecanal is influenced by the presence in the traps of accumulate specimens trapped. This fact has been observed both in trap containing alive or dead specimens. Interactions in the different substances produced by adults of Red Flour Beetle during their communication relationship are involved in this behaviour.The results obtained in the trials suggests that the presence of alive insects in traps is attractive for other free specimens, but under conditions of overcrowding quinone produced by them regulate population density by counteracting the effect of the aggregation pheromone, causing the insects to disperse or suppressing aggregation behaviour. 相似文献
7.
Giorgio Gambino Jeannette Bondaz Ivana Gribaudo 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(4):397-404
The distribution of some grapevine viruses in flower explants, embryogenic and non-embryogenic calli, single somatic embryos
and plants regenerated from embryogenic cultures was investigated by RT-PCR and ELISA. Immature anthers and ovaries of the
cultivars Grignolino infected by GRSPaV, GLRaV-1 and GVA, Müller-Thurgau infected by GRSPaV and GLRaV-3 and Bosco infected
by GRSPaV were cultivated on media inducing indirect somatic embryogenesis. Viruses were detected both in anthers and ovaries.
Four months after culture initiation 65.6% of tested calli were infected by at least one virus; high percentages of virus
infection were found in calli originating from ovaries. No virus was detected in calli tested 8 months after culture initiation,
as well as in single somatic embryos or in embryo-derived plantlets. Somatic embryogenesis confirmed its effectiveness in
eliminating phloem-limited grapevine viruses. Regeneration of RT-PCR negative plantlets occurred even when at least a sector
of the callus was still infected: the mechanism whereby somatic embryos are freed of some viruses could be related to the
rapid proliferation of embryogenic cells within the callus or to the origin of the embryogenic callus from virus-free cells
within the original explant. 相似文献
8.
G. La Rosa M. Muscillo A. Di Grazia S. Fontana M. Iaconelli M. Tollis 《Zoonoses and public health》2006,53(6):257-265
Porcine enteroviruses (PEVs) and teschoviruses (PTVs) are described as causative agents of neurological disorders, fertility disorders and dermal lesions of swine. Difficulties in the serological detection of these viruses may lead to a significant underestimation of infections with clinical symptoms. With the recent availability of genome sequence data for all the serotypes, molecular diagnosis is a possibility. The present study describes a new approach to molecular ‘serotyping’ of PTVs and PEV‐B viruses, involving the amplification and sequencing of a genomic fragment of the VP1 coding region. A molecular characterization of Italian entero‐teschovirus isolates was performed using a set of previously published and newly designed polymerase chain reaction primers. A total of 33 porcine isolates and 10 reference strains were analysed. Porcine enterovirus‐B samples were first diagnosed as positive for enterovirus by amplification of the 5′‐non‐translated region. Samples were then typed by amplification and sequencing of a portion of the VP1 coding region. Porcine enterovirus‐A and PTVs were detected by a published assay in the 5′‐NC region that allows them to be differentiated according to the size of amplification product, using the same set of primers. For serotype characterization of PTV, we evaluated four different regions: the N terminus of the capsid protein VP2, the region encoding for RNA‐dependent RNA polymerase, and the capsid VP1 and VP4 regions. The newly designed primers in the VP1 region was proved to be broad in range and suitable for serotype assessment and therefore constitute a useful diagnostic tool for molecular diagnosis of porcine teschovirus/enterovirus strains and for the study of molecular epidemiology and evolution of these viruses. 相似文献
9.
Agostino Sorgonà Maria Rosa AbenavoliPietro Giorgio Gringeri Giovanni Cacco 《Scientia Horticulturae》2006
Nitrogen use efficiency (NUE) definitions, commonly used in literature, are evaluated in response to nitrate availability in four citrus rootstocks, Rough Lemon (Citrus jambhiri Lush) (RL), Sweet Orange (Citrus sinensis (L.) Osbeck) (SwO), Cleopatra Mandarin (Citrus reshni Hort ex Tan.) (CM) and Sour Orange (Citrus aurantium L.) (SO). The application of diverse definitions determine different characterizations in N-efficiency among rootstocks. Nitrogen utilization efficiency (NUtE) and nitrogen efficiency ratio (NER) determine equal level of nitrogen efficiency among all rootstocks. Total nitrogen accumulation (TNA), nitrogen uptake efficiency (NUpE), shoot dry weight (SDW) and total leaf area (TLA) response curves produce the same NUE characterization: SO and SwO were nitrate use efficient and inefficient rootstocks, respectively, while the RL and CM exhibit superior and inferior genetic potential, respectively. 相似文献
10.
In the trapping ofTribolium castaneum (Herbst), the activity of traps baited or not baited with aggregation pheromone 4,8-dimethyldecanal is influenced by the presence in the traps of accumulate specimens trapped. This fact has been observed both in trap containing alive or dead specimens. Interactions in the different substances produced by adults of Red Flour Beetle during their communication relationship are involved in this behaviour.The results obtained in the trials suggests that the presence of alive insects in traps is attractive for other free specimens, but under conditions of overcrowding quinone produced by them regulate population density by counteracting the effect of the aggregation pheromone, causing the insects to disperse or suppressing aggregation behaviour.The presence of dead insects in the traps, baited or not baited with pheromone, can be repellent for other free specimens ofT. castaneum, probably related to the presence of a residual alarm pheromone produced by the specimens before of death.With 10 figures and 3 tables 相似文献