Clinical,pathologic, and toxicologic characterization of Salvia reflexa (lance-leaf sage) poisoning in cattle fed contaminated hay     

Kip E. Panter  Bryan L. Stegelmeier  Dale R. Gardner  Clinton A. Stonecipher  Stephen T. Lee  Don Kitchen  Adeline Brackett  Charlie Davis 《Journal of veterinary diagnostic investigation》2021,33(3):538

Salvia reflexa (lance-leaf sage)-contaminated alfalfa hay was fed to ~500 mixed-breed beef cattle. Within hours of exposure, nearly half of the cattle developed lethargy, anorexia, depression, and recumbency, followed by bellowing, colic, and death. Even though the uneaten contaminated hay was removed the first day, nearly 100 animals died within the first 48 h. Three of these cattle were examined postmortem, and tissues and hay samples were collected for microscopic and chemical analysis. Several days later, a smaller number of the clinically poisoned cattle developed neurologic disease with aberrant behavior, aggression, icterus, blindness, exhaustion, and death. A total of 165 cattle were fatally poisoned. Poisoned cattle had swollen, dark, mottled livers that had a prominent nutmeg-like lobular pattern on cut section. Histologically, there was severe centrilobular-to-panlobular hepatic necrosis with marked hepatocellular swelling, degeneration, and necrosis. The surviving cattle developed liver disease characterized by altered serum biochemical analyses and microscopic hepatocellular degeneration and necrosis. In subsequent biopsies and analysis, these lesions resolved within 6–7 mo. After confirming toxicity of the hay in cattle, goats, and mice, followed by a mouse bioassay–guided chemical fractionation process, Salvia reflexa was identified as the contaminant in the hay responsible for the hepatotoxicity. S. reflexa has not been reported previously to cause fatal hepatotoxicity in livestock in North America, to our knowledge.  相似文献   

Effect of farm characteristics and practices on hygienic quality of ovine raw milk used for artisan cheese production in central Italy          下载免费PDF全文

Elisa Carloni  Annalisa Petruzzelli  Giulia Amagliani  Giorgio Brandi  Francesco Caverni  Piermario Mangili  Franco Tonucci 《Animal Science Journal》2016,87(4):591-599

A survey on ovine dairy farms directly transforming own‐produced milk, in the Italian Marche region, was carried out to assess flock and milking practices that may influence milk hygienic‐sanitary conditions. A census survey established that 24 dairy farms were located in this region. Bulk milk samples were collected throughout the milking period in each dairy farm in 2013. Analyzed variables were: (i) chemical parameters such as fat, protein and lactose content, dry matter and pH; and (ii) total bacterial (TBC) and somatic cell counts (SCC). Chemical parameter values were in agreement with published data while, geometric mean (GM) log₁₀ SCC was 5.91 and TBC GM was 57 978 colony forming units/mL, in compliance with Eropean Union criteria. A positive correlation was found between SCC and TBC when GMs of all farm data were considered (Spearman's rho = 0.7925; P = 0.0001). Statistical analysis did not show significant correlation between SCC or TBC GM and dairy farm principal characteristics. Although SCC levels detected in the present study should suggest the need to implement mastitis control programs, Marche's dairy sheep flocks revealed a good hygienic condition level. This is an important aspect in implementing safety for end users of the final product.  相似文献   

Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology     

Chiara Gomiero  Giulia Bertolutti  Tiziana Martinello  Nathalie Van Bruaene  Sarah Y. Broeckx  Marco Patruno  Jan H. Spaas 《Veterinary research communications》2016,40(1):39-48

Tendons regenerate poorly due to a dense extracellular matrix and low cellularity. Cellular therapies aim to improve tendon repair using mesenchymal stem cells and tenocytes; however, a current limitation is the low proliferative potential of tenocytes in cases of severe trauma. The purpose of this study was to develop a method useful in veterinary medicine to improve the differentiation of Peripheral Blood equine mesenchymal stem cells (PB-MSCs) into tenocytes. PB-MSCs were used to study the effects of the addition of some growth factors (GFs) as TGFβ3 (transforming growth factor), EGF2 (Epidermal growth factor), bFGF2 (Fibroblast growth factor) and IGF-1 (insulin-like growth factor) in presence or without Low Level Laser Technology (LLLT) on the mRNA expression levels of genes important in the tenogenic induction as Early Growth Response Protein-1 (EGR1), Tenascin (TNC) and Decorin (DCN). The singular addition of GFs did not show any influence on the mRNA expression of tenogenic genes whereas the specific combinations that arrested cell proliferation in favour of differentiation were the following: bFGF2 + TGFβ3 and bFGF2 + TGFβ3 + LLLT. Indeed, the supplement of bFGF2 and TGFβ3 significantly upregulated the expression of Early Growth Response Protein-1 and Decorin, while the use of LLLT induced a significant increase of Tenascin C levels. In conclusion, the present study might furnish significant suggestions for developing an efficient approach for tenocyte induction since the external administration of bFGF2 and TGFβ3, along with LLLT, influences the differentiation of PB-MSCs towards the tenogenic fate.  相似文献   

Stress and Humoral Innate Immune Response of Gilthead Seabream Sparus aurata Cultured in Sea Cages     

Fulvio Salati  Giulia Angelucci  Alessandra Fenza  Adele Meluzzi 《Journal of aquatic animal health》2016,28(3):166-172

Innate and acquired immune responses of Gilthead Seabream Sparus aurata was studied under normal culture and short-term stressful conditions for 18 months in offshore sea cages in Alghero Bay, Italy. Every 45 d, 50 fish were sampled and divided into two groups: fish in the first group (normal culture conditions) were bled after harvesting; fish in the second group were put into a tank under stressful conditions (crowding and confinement) and bled after 2 h. Innate humoral immunity, such as complement-like, hemagglutination, and lysozyme activities, was determined in the sera of both groups. Pathogen challenge was not performed, but the specific humoral immune response was assessed against the most common pathogens affecting cultured fish in Sardinia. Stressed fish, compared with the control, showed a lower lysozyme activity against Vibrio (Listonella) anguillarum, which was not clearly correlated with temperatures. Complement-like activity differed between the first and second half of the study and, at the end of the trial, a slightly higher activity was recorded in the controls than in the stressed fish. Hemagglutination activity was mainly higher in the stressed fish than in control fish. Confinement, crowding, and cold water temperature caused decreased lysozyme activity in short-term stressed Gilthead Seabream compared with those reared normally. The specific humoral immune response, against V. anguillarum, Tenacibaculum mesophilum, Enterococcus Seriolicida, and Aeromonas sobria, fluctuated during the rearing period, particularly during the first year of culture.

Received October 12, 2015; accepted March 24, 2016 Published online August 2, 2016  相似文献   

Frequency of urinary tract infection in catheterized dogs and comparison of bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with urinary tract infections   总被引：2，自引：0，他引：2  

Bubenik LJ  Hosgood GL  Waldron DR  Snow LA 《Journal of the American Veterinary Medical Association》2007,231(6):893-899

OBJECTIVE: To determine frequency of urinary tract infections (UTIs) in catheterized dogs that had intervertebral disk disease (IVDD) or disease other than IVDD and compare bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with UTIs. DESIGN: Retrospective cohort study. ANIMALS: 147 catheterized dogs (105 with IVDD and 42 with other diseases) and 99 noncatheterized dogs with UTIs. PROCEDURES: Medical records were reviewed for signalment, history, clinical problem, duration of urinary tract catheterization, administration of drugs, and urine bacterial culture and susceptibility testing results. RESULTS: Forty-two percent (44/105) of dogs with IVDD and 55% (23/42) of dogs with other diseases had UTIs; this difference was not significant. For catheterized dogs, the odds of UTI were increased by 20% for each year increase in age, 27% for each day increase in duration of catheterization, and 454% with antimicrobial administration. Escherichia coli and Proteus spp were more frequently isolated from noncatheterized dogs, whereas Enterobacter spp and Staphylococcus spp were more frequently isolated from catheterized dogs. There was no significant difference in frequency of 1, 2, or 3 isolates between groups. Proportions of antimicrobials to which the most frequently isolated bacteria were resistant were not significantly different between groups. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that urinary tract catheterization is a reasonable alternative for management of dogs with urinary bladder dysfunction, but that duration of catheterization should be minimized and indiscriminate antimicrobial administration to dogs with indwelling urinary catheters should be avoided.  相似文献   

Radiographic,MRI, and CT findings in a young dog with Becker-like muscular dystrophy     

Giulia Costanzo  Sergio Minei  Eric Zini  Margherita Gracis  Giuseppe Lacava 《Veterinary radiology & ultrasound》2023,64(2):E19-E22

An 8-month-old, male, crossbreed dog was presented for macroglossia, reduced mandibular extension, ptyalism, dysphagia, and regurgitation. Serum creatine kinase and aspartate aminotransferase activity were markedly increased. Thoracic radiographs showed an axial gastro-esophageal hiatal hernia, diaphragmatic thickening, and asymmetry. Magnetic resonance imaging of the head showed a severely enlarged tongue, symmetric increase in size of the geniohyoid and mylohyoid muscles, and diffuse masticatory hypomyotrophy. Whole-body CT ruled out other musculoskeletal abnormalities and further characterized the radiographic and MRI findings. Muscular histopathology was consistent with Becker muscular dystrophy.  相似文献   

Novel soil quality indicators for the evaluation of agricultural management practices: a biological perspective     

Giulia BONGIORNO 《农业科学与工程前沿(英文版)》2020,7(3):257-274

Developments in soil biology and in methods to characterize soil organic carbon can potentially deliver novel soil quality indicators that can help identify management practices able to sustain soil productivity and environmental resilience. This work aimed at synthesizing results regarding the suitability of a range of soil biological and biochemical properties as novel soil quality indicators for agricultural management. The soil properties, selected through a published literature review, comprised different labile organic carbon fractions [hydrophilic dissolved organic carbon, dissolved organic carbon, permanganate oxidizable carbon (POXC), hot water extractable carbon and particulate organic matter carbon], soil disease suppressiveness measured using a Pythium-Lepidium bioassay, nematode communities characterized by amplicon sequencing and qPCR, and microbial community level physiological profiling measured with MicroResp^TM. Prior studies tested the sensitivity of each of the novel indicators to tillage and organic matter addition in ten European long-term field experiments (LTEs) and assessed their relationships with pre-existing soil quality indicators of soil functioning. Here, the results of these previous studies are brought together and interpreted relative to each other and to the broader body of literature on soil quality assessment. Reduced tillage increased carbon availability, disease suppressiveness, nematode richness and diversity, the stability and maturity of the food web, and microbial activity and functional diversity. Organic matter addition played a weaker role in enhancing soil quality, possibly due to the range of composition of the organic matter inputs used in the LTEs. POXC was the indicator that discriminated best between soil management practices, followed by nematode indices based on functional characteristics. Structural equation modeling shows that POXC has a central role in nutrient retention/supply, carbon sequestration, biodiversity conservation, erosion control and disease regulation/suppression. The novel indicators proposed here have great potential to improve existing soil quality assessment schemes. Their feasibility of application is discussed and needs for future research are outlined.  相似文献   

Detection and characterization of a rhabdovirus causing mortality in black bullhead catfish,Ameiurus melas          下载免费PDF全文

Giulia Bedendo  Valentina Panzarin  Andrea Fortin  Gianpiero Zamperin  Tobia Pretto  Alessandra Buratin  Rosita Quartesan  Matteo Sabbion  Cristian Salogni  Francesco Pascoli  Anna Toffan 《Journal of fish diseases》2018,41(7):1063-1075

This study fully describes a severe disease outbreak occurred in 2016 in black bullhead catfish farmed in Italy. Affected fish showed nervous clinical signs as well as emaciations and haemorrhagic petechiae on the skin at the fin bases, abdomen and gills. Viral isolation in cell culture allowed the subsequent identification of a rhabdovirus, tentatively named ictalurid rhabdovirus (IcRV), through electron microscopy, immunofluorescence and whole genome sequencing (WGS). The newly isolated virus, together with 14 additional viral strains stored in our repository and detected during similar mortality episodes in the period 1993–2016, was phylogenetically analysed on the basis of the nucleoprotein and the glycoprotein nucleotide and amino acid sequences. The genetic distances among Italian IcRV strains were also estimated. Our results show that all the IcRV strains belong to the genus Sprivivirus and are closely related to the tench rhabdovirus (TenRV). Italian catfish production is constantly decreasing, mainly due to viral infections, which include the newly characterized IcRV. Data presented in this work will assist to investigate the molecular epidemiology and the diffusive dynamics of this virus and to develop adequate surveillance activities.  相似文献   

The feasibility of oyster raft culture in east coast estuaries     

Glenn Aprill  Don Maurer 《Aquaculture (Amsterdam, Netherlands)》1976,7(2):147-160

Styrofoam-floated oyster rafts were used to study the feasibility of raft culture in typical American east coast estuaries. Rafts were placed in Delaware Bay and several tidal tributaries in the state of Delaware. Four types of cultch were tested for resistance to the environment and utilization by setting spat. Hatchery-spawned oysters were reared on rafts and their growth and mortality were monitored. A special raft with moveable trays was designed to study the effectiveness of air drying in controlling fouling.Rafts withstood the environmental conditions in small tidal rivers but were severely damaged by waves in Delaware Bay. Although spat set was too low for commercial purposes in the rivers, sets approaching commercial density were obtained in the bay by putting bundles of strings on the oyster beds during setting. Oysters grown on rafts reached market size in approximately 2 years in two of the rivers: slower growth was noted in the other rivers. It was shown that fouling could be controlled effectively by air drying for 4 h each week.An economic analysis of raft culture for this area was conducted at the conclusion of the study, which showed that relatively high prices would be necessary to maintain a raft-based industry at this time.  相似文献   

Effects of multiple fires on tree invasion in montane grasslands     

Russell Fairfax  Rod Fensham  Don Butler  Kelvin Quinn  Bernice Sigley  James Holman 《Landscape Ecology》2009,24(10):1363-1373

There is circumstantial evidence that grasslands on the Bunya Mountains were once maintained by Aboriginal burning, and with lack of fire under European management are being colonised by trees. To assess the efficacy of burning for maintaining grasslands, 119 fires were lit between 1996 and 2006. The total area of unburnt grasslands decreased by 27%, while grasslands burnt at least once decreased by 1%. The density of invading trees was recorded from fixed plots on 23 grasslands burnt between one and six times. Cassinia was virtually eliminated and the density of the Rainforest species guild slowly but continually declined. Acacia irrorata exhibited a humped response, with initial increases resulting from vegetative resprouting and gradual decline with persistent burning. Phyllodinous Acacia and Woodland trees were the least fire sensitive guilds, having stable or increased density with repeated burning. Multi-factor regression modelling detected no significant relationships between changes in woody plant density and the interval between fires, fire intensity, the initial density of large trees, an index of soil moisture, or the cumulative number of fires for any species guild. The survivorship of both Cassinia and Rainforest guilds was significantly lower with summer burning than winter burning, but a seasonal effect of burning was not evident for other guilds. The findings suggest that regardless of fire conditions, frequent burning will reduce the number of adult trees, maintain resprouts in an immature state, facilitate further fire and reduce the rate of grassland loss. Woodland species are especially resilient to fire, and burning to maintain grassy ecosystems will be most successful where the main colonisers are rainforest species and burning is conducted in summer. The findings suggest that the montane grasslands of the Bunya Mountains were maintained by anthropogenic burning and active fire management will prolong their existence.  相似文献