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Three accessions, corresponding to different ‘foundation’ farms, of each of the alfalfa (lucerne) Italian ecotypes ‘Vogherese’ and ‘Marem‐mano’, and two varieties were studied in order to evaluate: (1) the among‐ and within‐ecotype variation; and (2) the possibility of distinguishing the populations by means of bio‐agronomic characters and molecular markers (amplified fragment length polymorphisms, AFLPs). The two ecotypes could be distinguished by the differences in persistency after 15 cuttings in 3 years cultivation and by the seasonal distribution of the dry matter yield. Within‐ecotype variation was always of minor importance as compared with the among‐ecotype differences. The AFLP analyses generated 20 polymorphic fragments in ‘bulk’ samples: none was able to distinguish the two ecotypes, while variety‐specific fragments were found. The same analysis on an individual plant basis (485 polymorphic fragments) confirmed the absence of differences among ecotypes, while 10% of the total variance was attributable to variation among accessions within ecotype. The results of the analyses using agronomic traits and molecular markers are discussed in relation to the possibility of ecotype identification.  相似文献   
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Plant Foods for Human Nutrition - In the past few years, evidence has supported the role of plants as a valuable tool for the development of promising therapeutic support options for many diseases,...  相似文献   
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Plant Foods for Human Nutrition - Nowadays, a growing body of evidence supports the view that plants offer an extraordinary opportunity to discover and develop new promising therapeutic strategies...  相似文献   
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M. Carelli    G. Gnocchi    C. Scotti 《Plant Breeding》2009,128(3):271-277
In North African oases Medicago sativa ssp. sativa is likely to have been cultivated for long time without contacts with ssp. falcata . In order (i) to verify the genetic distinctiveness of the alfalfa germplasm of the oasis and (ii) to examine their internal variation, 17 farm landraces were collected in Siwa oasis (Egypt) and studied with four Egyptian and three Italian cultivars (120 plants/population) by means of bio-agronomic characters in 2 years cultivation and SSR markers in 'bulk' samples. Siwa germplasm was similar to the cultivars for dry matter yield but differed for plant form, earliness and reaction to fall conditions; SSR analysis based on 31 polymorphic DNA fragments distinguished Siwa landraces from the two groups of varieties. The variation within Siwa group was of continuous type based on bio-agronomic traits; average genetic similarity was high (0.8959) and UPGMA dendrogram suggests poor isolation among the landraces, although all, except two, were uniquely fingerprinted. The use of putative 'pure' subspecies of high agronomic value in the alfalfa variety construction process is discussed.  相似文献   
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