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1.
Hani A. Alhadrami Gaia Burgio Bathini Thissera Raha Orfali Suzan E. Jiffri Mohammed Yaseen Ahmed M. Sayed Mostafa E. Rateb 《Marine drugs》2022,20(3)
The COVID-19 pandemic and its continuing emerging variants emphasize the need to discover appropriate treatment, where vaccines alone have failed to show complete protection against the new variants of the virus. Therefore, treatment of the infected cases is critical. This paper discusses the bio-guided isolation of three indole diketopiperazine alkaloids, neoechinulin A (1), echinulin (2), and eurocristatine (3), from the Red Sea-derived Aspergillus fumigatus MR2012. Neoechinulin A (1) exhibited a potent inhibitory effect against SARS-CoV-2 Mpro with IC50 value of 0.47 μM, which is comparable to the reference standard GC376. Despite the structural similarity between the three compounds, only 1 showed a promising effect. The mechanism of inhibition is discussed in light of a series of extensive molecular docking, classical and steered molecular dynamics simulation experiments. This paper sheds light on indole diketopiperazine alkaloids as a potential structural motif against SARS-CoV-2 Mpro. Additionally, it highlights the potential of different molecular docking and molecular dynamics simulation approaches in the discrimination between active and inactive structurally related Mpro inhibitors. 相似文献
2.
Valérie Gravel Hani Antoun Russell J. Tweddell 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(4):457-462
The effect of indole-acetic acid (IAA) on the development of symptoms caused by Pythium ultimum on tomato plants was investigated using different bioassays. Application of IAA (5 μg ml−1) on tomato seedlings inoculated with P. ultimum did not affect their emergence suggesting that IAA did not affect the severity of Pythium damping-off. However, IAA was shown
to influence the development of P. ultimum symptoms on tomato plantlets. Low concentrations of IAA (0–0.1 μg ml−1) within the rhizosphere of plantlets increased the severity of the symptoms caused by P. ultimum, while higher concentrations (10 μg ml−1), applied either by drenching to the growing medium or by spraying on the shoot, reduced the symptoms caused by this pathogen.
In addition, the study demonstrated that P. ultimum produces IAA in liquid culture amended with L-tryptophan, tryptamine or tryptophol (200 μg ml−1) and in unamended culture. 相似文献
3.
Chikaya Sakai Atok Subiakto Hani Sitti Nuroniah Naoto Kamata Koji Nakamura 《Journal of Forest Research》2002,7(2):73-80
We developed a vegetative propagation system for mass-producing three dipterocarps species,Shorea selanica Bl.,Shorea leprosula Mig., andShorea platyclados Sloot. This system uses fog evaporative cooling inside a greenhouse to reduce the leaf-to-air vapor pressure deficit (leaf-to-air
VPD) inside the propagator, even under high irradiance conditions. This cooling method has no negative influence on medium
conditions such as overwetting. A plastic tent propagator combined with this cooling method was used for vegetative propagation
experiments. In mass-production experiments, the annual rooting percentages from the cuttings were low in the first 2 years
(1997–1998) due to operational problems of the tent propagator (S. selanica, 48–51%;S. leprosula, 56–59%;S. platyclados: 50–63%). A hard cover propagator improved the rooting percentages in the mass-production experiments in 1999 because it
made operations easier (S. selanica, 70%;S. leprosula, 77%;S. platyclados, 77%). This system, which uses a combination of fog evaporative cooling and a hard plastic propagator, should be useful for
the mass propagation of these dipterocarp planting stocks.
This study was carried out under a joint project of the Ministry of Forestry, Indonesia and Komatsu Ltd. The project was supported
by Research Association for Reforestation of Tropical Forest (RETROF) organized by Japanese Forestry Agency. 相似文献
4.
Peter D. Constable BSc Phd Robert N. Streeter Gerard J. Koenig Nigel R. Perkins Hani M. Gohar Dawn E. Morin 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1997,11(2):71-79
The objectives of this study were to investigate the determinants of the anion gap (AG) in cattle and to evaluate the utility of AG in detecting hyperlactatemia in sick neonatal calves and adult cattle. The AG was calculated as AG = ([Na+] + [K+]) - ([Cl-] + [HCO3]), with all values in mEq/L. The AG of healthy neonatal calves (n = 16) was 29.6 ± 6.2 mEq/L (mean ± SD), and the blood L-lactate concentration ranged from 0.5 to 1.2 mM/L. The AG was significantly (P > .05) correlated with serum phosphate (r = .66) and creatinine (r = .51) concentrations. The AG of neonatal calves with experimentally induced diarrhea (n = 16) was 28.6 ± 5.6 mEq/L, and the blood L-lactate concentration ranged from 1.1 to 2.9 mM/L. The AG was significantly correlated with blood L-lactate concentration (r = .67), serum phosphate concentration (r = .63), creatinine concentration (r = .76), and blood pH (r = -.61). The AG of adult cattle with abomasal volvulus (n = 41) was 20.5 ± 7.8 mEq/L, and the blood L-lactate concentration ranged from 0.6 to 15.6 mM/L. The AG was significantly correlated with blood L-lactate concentration (r = .60), serum phosphate concentration (r = .71), creatinine concentration (r = .65), albumin concentration (r = .47), total protein concentration (r = .54), blood pyruvate concentration (r = .67), and blood pH (r = -.41) but not plasma β-OH butyrate concentration. The results indicate that the AG in cattle is only moderately correlated with blood L-lactate concentration and is similarly correlated with serum phosphate and creatinine concentrations in neonatal calves and adult cattle, as well as with serum albumin and total protein concentrations in adult cattle. Anion gap determination is of limited usefulness in predicting blood L-lactate concentration in sick cattle, whereas the correlation between AG and serum creatinine concentration in sick cattle suggests that an increased AG should alert the clinician to the potential presence of uremic anions. 相似文献
5.
最近 ,美国联合蛋业公司出版了一本小册子 ,名为《美国产蛋母鸡群饲养管理指南》 (ＡｎｉｍａｌＨｕｓ ｂａｎｄｒｙＧｕｉｄｅｌｉｎｅｓｆｏｒＵ Ｓ ＥｇｇＬａｙｉｎｇＨｅｎＦｌｏｃｋｓ)该书内容来自该公司科学顾问委员会的建议 (该委员会由动物行为学、生理学和饲养管理领域资深学术界和非学术界人士组成 )。书中有大量对养鸡者非常有用的信息。本文就摘自这本小册子。科学证据表明 ,从行为学的观点来看 ,产蛋鸡育种者可选育温驯的个体以减少进行断喙的需要。采用几乎不需要断喙的鸡种 ,是最好的办法。然而 ,在某些管理条件下 (比如 ,… 相似文献
6.
In continuation of our search for drug leads from Red Sea sponges we have investigated the ethyl acetate fraction of the organic extract of the Red Sea sponge Hyrtios species. Bioassay-directed fractionation of the active fraction resulted into the identification of three new alkaloids, hyrtioerectines D–F (1–3). Hyrtioerectines D–F belong to the rare marine alkaloids in which the indole and β-carboline fragments of the molecule are linked through C-3/C-3 of both moieties. The structures of the isolated compounds were established based on different spectroscopic data including UV, IR, 1D and 2D NMR (COSY, HSQC, and HMBC) and high-resolution mass spectral studies. The antimicrobial activity against several pathogens and the free radical scavenging activity of the compounds using DPPH reagent were evaluated. In addition, the growth inhibitory activity of the compounds against three cancer cell lines was also evaluated. Hyrtioerectines D–F (1–3) displayed variable antimicrobial, free radical scavenging and cancer growth inhibition activities. Generally, compounds 1 and 3 were more active than compound 2. 相似文献
7.
Fatemah Kharazmi Nepton Soltani Sana Rezaei Mansoor Keshavarz Leila Farsi 《Iranian Biomedical Journal》2015,19(2):91-95
Background:
Hypertension is considered an independent risk factor for cardiovascular mortality in diabetic patients. The present study was designed to determine the role of gamma amino butyric acid B (GABAB) receptor and L-arginine (L-Arg) in GABA-induced vasorelaxation in normal and streptozotocin-induced diabetic rat vessels.Methods:
Diabetes was induced by a single i.p. injection of streptozotocin (STZ, 60 mg/kg). Eight weeks later, superior mesenteric arteries of all groups were isolated and perfused according to the McGregor method.Results:
Baseline perfusion pressure of STZ diabetic rats was significantly higher than non-diabetic rats in both intact and denuded endothelium. In the presence of faclofen, a selective GABAB receptor blocker, GABA-induced relaxation in intact and denuded endothelium mesenteric beds of STZ diabetic rats was suppressed, but this response in non-diabetic rats was not suppressed. Our results showed that in the presence of L-Arg, a nitric oxide precursor, GABA induced vasorelaxation in both diabetic and non-diabetic vessels.Conclusion:
From the results of this study, it may be concluded that the vasorelaxatory effect of GABA in diabetic vessel is mediated by the GABAB receptor and nitric oxide, but it seems that in non-diabetic vessel GABAB receptor does not play any role in GABA-induced vasorelaxation, but nitric oxide induced GABA relaxation in non-diabetic vessel. Key Words: Gamma amino butyric acid (GABA), Diabetes, GABAB receptor 相似文献8.
Bioassay-guided partition of the extract of the Red Sea sponge Pseudoceratina arabica and HPLC purification of the active fraction gave a psammaplysin dimer, psammaceratin A (1), along with psammaplysin A (2). The dimer comprises two units of psammaplysin A (2) connected via the terminal amines with an unprecedented (2Z,3Z)-2,3-bis(aminomethylene)succinamide moiety, and it represents the first dimer to be identified among the psammaplysin family. Data from 1D- and 2D-NMR and HRMS supported the chemical structures of the compounds. Psammaceratin A (1) and psammaplysin A (2) exhibited significant growth inhibition of HCT 116, HeLa, and MBA-MB-231 cells down to 3.1 μM. 相似文献
9.
Mosavari N Feizabadi MM Jamshidian M Shahpouri MR Forbes KJ Pajoohi RA Keshavarz R Taheri MM Tadayon K 《Veterinary microbiology》2011,151(1-2):148-152
Restriction fragment length polymorphism (RFLP) genotyping was employed to analyze the population genetics of Mycobacterium bovis in Iran. One hundred and twenty-three isolates collected from slaughtered tuberculosis-suspect cattle and one clinically asymptomatic buffalo were subjected to RFLP analysis with probes of the polymorphic GC-rich sequence (PGRS) and the direct repeat sequence (DR) using DNA digested with PvuII and AluI. All these methods detected a large homogeneous population in which only a few isolates had variant genotypes. Only AluI-based RFLPs of both the PGRS and DR sequences were able to clearly differentiate between BCG and field strains of M. bovis. As in previous reports, these findings seem to reflect a recent dispersal of one or a few strains in Iran following the substantial expansion of Holstein-Friesian cattle over the last few decades. 相似文献
10.
Tadayon K Forbes K Soleimani D Pajoohi RA Marhmati B Javidi J Keshavarz R Soleimani K Shakibamehr A Taheri MM Pour SD Mosavari N 《Veterinary microbiology》2011,151(1-2):192-199
In 1931 Carpantier reported bovine TB (BTB) in Iranian cattle. Some eighty years on with a national test-and-slaughter programme in place for over four decades, the efforts to vanquish Mycobacterium bovis (M. bovis) infection in cattle have been in vain as the vast majority of the 30 Iranian provinces still have reports of BTB in their cattle herds every year. This paper reviews the present epidemiology of BTB in Iran and in the region and evaluates the success of government policy in controlling this disease. 相似文献