Induction of superovulation by immunoneutralization of endogenous inhibin in immature rats     

Ishigame H  Medan MS  Kawaguchi M  Fukuda A  Watanabe G  Arai KY  Taya K 《The Journal of reproduction and development》2005,51(5):559-566

The effects of passive immunoneutralization of endogenous inhibin on ovulation rate in immature rats were investigated. Efficiency of superovulation on production of fertilized oocytes was compared between the inhibin antiserum (inhibin-AS) and equine chorionic gonadotropin (eCG) protocols. Immature female Wistar strain rats were superovulated with a single injection of 100-200 microl inhibin-AS, with and without an injection of human chorionic gonadotropin (hCG). A total of 77.8% of the 26-30-day-old rats treated with a single injection of 100-200 microl inhibin-AS ovulated 72 h after treatment, while rats given normal goat serum (NGS; 200 microl) did not ovulate. At 28 days of age, all of the inhibin-AS treated rats ovulated when additional hCG treatment was given, whereas the number of ovulated oocytes was not affected. The number of ovulated oocytes in the inhibin-AS-hCG treated groups was significantly higher than that of the NGS-hCG treated group. In addition, plasma concentrations of FSH in the inhibin-AS-hCG treated group significantly increased compared with the NGS treated group. While the percentage of mated rats in the 200 microl inhibin-AS-hCG treated group was significantly lower than that of the 15 IU eCG-hCG treated group, the fertilization rate was comparable between the two groups. The number of fertilized oocytes in the 200 microl inhibin-AS-hCG treated group was significantly higher in comparison with the 15 IU eCG-hCG treated group. These results suggest that immunoneutralization of endogenous inhibin could be a reliable method for induction of superovulation to collect a large number of normally fertilized oocytes in immature rats.  相似文献   

Effect of the concentration of NaCl and cylinder height on the sedimentation of flocculated suspension of Na-montmorillonite in the semi-dilute regime     

Ghazali  Muhamad Ezral Bin  Argo  Yanuar  Kyotoh  Harumichi  Adachi  Yasuhisa 《Paddy and Water Environment》2020,18(2):309-316

Paddy and Water Environment - In this study, we focused on the effect of the degree of floc growth on the moving velocity of the interface between sediment of flocculated montmorillonite and...  相似文献   

Clinical features and their course of pituitary carcinoma with distant metastasis in a dog     

Munekazu NAKAICHI  Toshie ISERI  Hiro HORIKIRIZONO  Yusuke SAKAI  Harumichi ITOH  Hiroshi SUNAHARA  Kazuhito ITAMOTO  Kenji TANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1671

An 11-year-old male toy poodle with neurological symptoms was diagnosed with a macroscopic pituitary tumor, which produced adrenocorticotropic hormone. Radiation therapy with a linear accelerator was performed for the pituitary tumor, and resulted in good local tumor control. However, serum endogenous adrenocorticotropic hormone concentrations were uncontrollable even after the tumor disappeared. Abdominal computed tomography revealed splenic masses, and splenectomy was performed. Histopathological examination of the surgical specimen showed tumor cells with eosinophilic and finely granular cytoplasm suggestive of endocrine origin. Since these cells were positive for adrenocorticotropic hormone, the case was diagnosed as a pituitary carcinoma with distant metastasis. Necropsy revealed multiple metastases to the abdominal organs. This is the first case report describing canine pituitary carcinoma with distant metastasis.  相似文献   

Effect of drug-eluting bead transarterial chemoembolization loaded with cisplatin on normal dogs     

Ko NAKASUMI  Naoki YAMAMOTO  Taro TAKAMI  Harumichi ITOH  Kazuhito ITAMOTO  Hiro HORIKIRIZONO  Toshie ISERI  Munekazu NAKAICHI  Yuki NEMOTO  Hiroshi SUNAHARA  Kenji TANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):114

Transcatheter arterial embolization (TAE) and transcatheter arterial chemoembolization (TACE) are standard treatments for advanced hepatocellular carcinoma (HCC) and particularly for unresectable tumors or liver metastases in humans. However, reports on TACE used in veterinary medicine are few. This study aimed to evaluate the feasibility and safety of drug-eluting bead transarterial chemoembolization (DEB-TACE). We performed DEB-TACE in four clinically normal dogs and pharmacokinetically compared the results against hepatic arterial infusion (HAI) of cisplatin in two dogs. Drug-eluting beads (DEB) loaded with cisplatin were injected through a microcatheter for selective embolization of the left hepatic artery. After embolization, computed tomography (CT) images and histological examination findings were obtained during a 4-week observation period. Serum platinum concentrations were measured to evaluate cisplatin after each procedure. Biochemical analysis was performed during a 12-week observation period. Embolization was successful in all dogs, and there were no clinically apparent abnormalities. Embolization was confirmed up to 4 weeks after DEB-TACE in two of the four dogs and up to 1 week in the other two dogs using postoperative CT images. Cisplatin was not detected in peripheral veins in all dogs after DEB-TACE, but it was detected in trace amounts after HAI. DEB-TACE using cisplatin was safe and well tolerated by normal dogs. DEB-TACE may be useful in terms of determining systemic toxicity and drug concentration within tumors.  相似文献   

Copper-associated hepatitis in a young Dalmatian dog in Japan     

Munekazu NAKAICHI  Toshie ISERI  Hiro HORIKIRIZONO  Misa KOMINE  Harumichi ITOH  Hiroshi SUNAHARA  Yuki NEMOTO  Kazuhito ITAMOTO  Kenji TANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):911

A male 25-month-old Dalmatian dog attended our veterinary hospital because of anorexia and high circulating liver enzyme activities. Abdominal computed tomography showed a slightly small liver with rounded edges, and laparoscopic examination showed that the liver was yellowish. Histopathological examination revealed multifocal necrosis of hepatocytes and severe chronic hepatitis. Rhodanine staining showed severe copper accumulation in hepatocytes and a quantitative analysis of the copper content of the liver showed substantial accumulation (10.3 mg/g dry mass), suggesting a diagnosis of copper-associated hepatitis. Previously reported canine mutation in the COMMD1, the gene responsible for the copper-associated hepatitis in the Bedlington terrier, was not identified. To our knowledge, this is the first report of copper-associated hepatitis in a Dalmatian in Japan.  相似文献