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1.
Brumbaugh GW Herman JD Clancy JS Burden KI Barry T Simpson RB López HS 《American journal of veterinary research》2002,63(1):36-41
OBJECTIVE: To evaluate chemotactic, phagocytic, and bactericidal activities of bovine and porcine alveolar macrophages (AM) exposed to tilmicosin. ANIMALS: 12 healthy calves and 12 healthy pigs. PROCEDURES: Lungs were obtained immediately after euthanasia; AM were collected by means of bronchoalveolar lavage and density gradient centrifugation. Chemotactic activity was evaluated by exposing AM to lipopolysaccharide or macrophage inhibitory peptide during incubation with tilmicosin. Phagocytic activity was evaluated by incubating AM with tilmicosin for 24 hours and then with tilmicosin-resistant Salmonella serotype Typhimurium. Bactericidal activity was evaluated by incubating AM with tilmicosin (0, 10, or 20 microg/ml for bovine AM; 0 or 10 microg/ml or 10 microg/ml but washed free of tilmicosin for porcine AM) and then with Mannheimia haemolytica (bovine AM) or with Actinobacillus pleuropneumoniae or Pasteurella multocida (porcine AM). RESULTS: Tilmicosin had no significant effects on chemotactic or phagocytic activities of bovine or porcine AM. The time-course of bactericidal activity was best described by polynomial equations. Time to cessation of bacterial growth and area under the time versus bacterial number curve were significantly affected by incubation of AM with tilmicosin. CONCLUSIONS AND CLINICAL RELEVANCE: Results show that bactericidal activity of bovine and porcine AM was enhanced by tilmicosin, but not in proportion to the reported ability of AM to concentrate tilmicosin intracellularly. With or without exposure to tilmicosin, the time-course of bactericidal activity of bovine AM against M haemolytica and of porcine AM against A pleuropneumoniae or P multocida was too complex to be reduced to a simple linear equation. 相似文献
2.
The antihyperglycemic effects of chemically synthesized d-chiro-inositol (d-CI), a component of an insulin mediator, have been demonstrated in rats. Buckwheat contains relatively high levels of d-CI: thus, it has been proposed as a source of d-CI for reducing serum glucose concentrations in diabetics. The present study evaluates the effects of a buckwheat concentrate, containing d-CI, on hyperglycemia and glucose tolerance in streptozotocin (STZ) rats. In fed STZ rats, both doses of the buckwheat concentrate (containing 10 and 20 mg of d-CI/kg of body weight) were effective for lowering serum glucose concentrations by 12-19% at 90 and 120 min after administration. Findings from this study demonstrate that a buckwheat concentrate is an effective source of d-CI for lowering serum glucose concentrations in rats and therefore may be useful in the treatment of diabetes. 相似文献
3.
Alison M. Miller Julianne M. O’Reilly-Wapstra Brad M. Potts Clare McArthur 《New Forests》2011,42(3):301-316
Mammalian herbivores regularly browse plantation seedlings. In many areas, seedlings require some form of protection if they
are to survive and grow into a productive plantation. Two general approaches for reducing browsing damage to seedlings are
to apply chemical repellents and tree guards. Both methods have existed for a long time, and new variations are constantly
being developed. Seedling stocking guards, a type of tree guard, are being used operationally in Tasmania, Australia, but
there is limited data quantifying their effectiveness and concerns with negative effects on tree performance. Conversely,
although proven effective, repellents are not being used, but are potentially cheaper and less problematic. We therefore aimed
to determine which is more effective under operational conditions, whether this effectiveness can be improved or extended,
and if either treatment has any effects on seedling form or survival. We planted Eucalyptus nitens seedlings with combinations of repellent and stocking guards in six operational plantations to examine and compare their
effectiveness. Seedlings were monitored for 12 months to examine treatment longevity. We found that both stocking guards and
repellent significantly reduced and delayed browsing severity, with their effects being additive. No negative effects on growth
were evident after 12 months, but adverse effects on seedling form warrant further investigation. Both of these methods can
be easily and relatively cheaply applied in the nursery before planting, making them appealing options to reduce browsing.
However, the ideal method for a given site will depend upon local browsing intensity. 相似文献
4.
Alison M. Miller Julianne M. O’Reilly-Wapstra Brad M. Potts Clare McArthur 《Forest Ecology and Management》2011,262(8):1500-1506
Browsing by mammalian herbivores is a major problem in plantation forestry. Seedlings are most vulnerable to browsing during establishment, making protection crucial during this period. Aside from reducing herbivore numbers, browsing can be controlled through the application of tree guards or chemical repellents. These methods are generally short-term options. A promising, potentially longer-term method of reducing browsing damage on plantations is to plant individuals with enhanced natural browse resistance. This requires the development of a rapid, cost-effective means of identifying germplasm with enhanced resistance. Here we present such a screening methodology.We planted Eucalyptus globulus seedlings from 22 different seedlots in randomised blocks along edge rows of six operational plantations. Seedlings were monitored for mammal browsing damage and growth for 2 years. Natural variation between E. globulus seedlots resulted in significant variation in the extent to which they were browsed. Differential browsing was evident just 10 weeks after planting, and caused a significant effect on tree growth after 2 years. Differential browsing was thought to be due to variation in levels of plant secondary metabolites (PSMs). The identification and selective planting of high PSM seedlots in high risk areas could be an effective management tool to reduce browsing in plantation forestry. 相似文献
5.
Modelling individual variability in growth of bull trout in the Walla Walla River Basin using a hierarchical von Bertalanffy growth model 
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下载免费PDF全文 Julianne E. Harris Courtney Newlon Philip J. Howell Ryan C. Koch Steven L. Haeseker 《Ecology of Freshwater Fish》2018,27(1):103-115
We examined growth in length of fluvial bull trout (Salvelinus confluentus) in the Walla Walla River Basin, Washington and Oregon. Our objectives were to quantify individual variability in growth; examine growth within and among years, life history forms, life stages and sexes; and estimate von Bertalanffy growth parameters. Individual variability was evaluated by modelling asymptotic length (L∞) and the growth coefficient (k) as random variables. All models were fit with Bayesian methods and were evaluated for fit by the deviance information criterion. By incorporating individual variability, population‐level estimates of L∞ and k appeared appropriate and estimated growth trajectories for specific bull trout fit individual observed patterns in growth. Growth trajectories and positive correlation between individual estimates of L∞ and k suggest that some individuals grow at a faster rate and reach a larger maximum size than other individuals and those differences are maintained throughout life. Selected models suggest that fluvial migrants have higher estimates of L∞ and k than residents, but there were only slight differences in parameter estimates among migrants from two adjacent spawning populations in the Walla Walla River Basin, as well as between males and females. Growth rates increased for fluvial migrants after subadult emigration. Individual variability in growth is consistent with the life history diversity assumed essential for bull trout population persistence. Quantifying this variability is important for modelling population dynamics and viability to conserve this threatened species. 相似文献
6.
Alessandro Busetti George Shaw Julianne Megaw Sean P. Gorman Christine A. Maggs Brendan F. Gilmore 《Marine drugs》2015,13(1):1-28
Bacterial epiphytes isolated from marine eukaryotes were screened for the production of quorum sensing inhibitory compounds (QSIs). Marine isolate KS8, identified as a Pseudoalteromonas sp., was found to display strong quorum sensing inhibitory (QSI) activity against acyl homoserine lactone (AHL)-based reporter strains Chromobacterium violaceum ATCC 12472 and CV026. KS8 supernatant significantly reduced biofilm biomass during biofilm formation (−63%) and in pre-established, mature P. aeruginosa PAO1 biofilms (−33%). KS8 supernatant also caused a 0.97-log reduction (−89%) and a 2-log reduction (−99%) in PAO1 biofilm viable counts in the biofilm formation assay and the biofilm eradication assay respectively. The crude organic extract of KS8 had a minimum inhibitory concentration (MIC) of 2 mg/mL against PAO1 but no minimum bactericidal concentration (MBC) was observed over the concentration range tested (MBC > 16 mg/mL). Sub-MIC concentrations (1 mg/mL) of KS8 crude organic extract significantly reduced the quorum sensing (QS)-dependent production of both pyoverdin and pyocyanin in P. aeruginosa PAO1 without affecting growth. A combinatorial approach using tobramycin and the crude organic extract at 1 mg/mL against planktonic P. aeruginosa PAO1 was found to increase the efficacy of tobramycin ten-fold, decreasing the MIC from 0.75 to 0.075 µg/mL. These data support the validity of approaches combining conventional antibiotic therapy with non-antibiotic compounds to improve the efficacy of current treatments. 相似文献
7.
Alison M. Miller Julianne M. O’Reilly-Wapstra Brad M. Potts Clare Mcarthur 《Forest Ecology and Management》2009
Browsing by mammalian herbivores is a major problem in plantation forestry worldwide. This has traditionally been controlled through a reduction in herbivore numbers achieved by lethal means, namely shooting and poisoning. In recent years, however, there has been increasing social and political pressure to reduce reliance on such lethal controls, and consequently research into non-lethal controls is becoming increasingly important. In order for non-lethal methods to be efficiently incorporated into management, however, we must first identify those that are most effective under operational conditions. Different methods are rarely tested concurrently, and many have only been proven effective with captive animals or in small scale field trials. This study therefore sought to consolidate past research by combining non-lethal methods, which were known to significantly reduce browsing damage, into a single trial to enable their relative effectiveness to be compared under operational conditions. 相似文献
8.
An account of air pollution control measures in the province of Ontario and their effectiveness is given. An experiment, currently in progress, to investigate the nature of thin layer brown haze which occasionally occurs over Toronto in the summer months in inversion and low wind conditions, is described. Preliminary results of the Aitken nuclei concentrations, droplet concentrations and size distributions, and NO2 concentrations found over Toronto in early morning and early afternoon flights on a day in August 1973 are given. 相似文献
9.
Jillian M. Haines Pamela M. Lee Rachel M. Hegedus Julianne K. Hwang Michael H. Court 《Journal of veterinary pharmacology and therapeutics》2019,42(2):160-170
Antiplatelet effects of acetylsalicylic acid (ASA, aspirin) may be poor in some individuals. Additionally, no method exists for predicting poor ASA response (resistance) in individual dogs. This study's main objective was to determine whether poor ASA response results from pharmacodynamic or pharmacokinetic causes. ASA concentrations causing 50% inhibition of platelet aggregation (in vitro IC50) were determined using whole blood collected from 21 drug‐free healthy dogs to evaluate intrinsic sensitivity of platelets to ASA. Dogs were then administered ASA at 4 mg/kg once orally. Percent decrease in platelet aggregation from baseline, and plasma ASA and salicylic acid (SA) concentrations (expressed as AUC values) were measured for up to 3 hr. By 3 hr, 13/21 (62%) dogs showed >50% aggregation inhibition, while 8/21 (38%) dogs showed <50% inhibition. Aggregation inhibition values were negatively correlated with in vitro IC50 values (Rs = ?0.49; p = 0.028) and positively correlated with ASA concentrations (Rs = 0.48; p = 0.03). Furthermore, ASA concentrations were strongly negatively correlated (Rs = ?0.88; p < 0.001) with SA/ASA concentration ratios, an index of ASA metabolism to SA by esterase enzymes. Multiple linear regression analysis indicated that 59% (p < 0.001) of interindividual variability in aggregation inhibition was explained by in vitro IC50 values (29% of variability) and ASA concentrations (29% of variability). Consequently, poor in vivo ASA response in these dogs resulted from both pharmacodynamic (decreased platelet sensitivity) and pharmacokinetic (lower ASA concentrations) causes. Lower ASA concentrations may be explained by reduced bioavailability associated with higher esterase activities. 相似文献
10.
Julianne Meisner Kellie Curtis Thomas W. Graham Michael B. Apamaku Lisa E. Manhart Peter M. Rabinowitz 《Zoonoses and public health》2019,66(1):73-82
Tuberculosis (TB) is a leading infectious cause of human death worldwide. In cattle, TB infection results in productivity losses, trade barriers and zoonotic transmission via milk, meat or direct contact. We conducted a cross‐sectional study in rural communities in Uganda between 2014 and 2016 to evaluate the association between tuberculosis skin test (TST) positivity in humans and cattle‐associated risk factors: household herd positivity and raw milk consumption. Human and cattle TSTs were performed in communities followed by a survey of household practices. TST data were available on 493 humans, 184 (37.3%) with positive results, and 1,441 cattle, 50 (3.5%) with positive results. We fit separate log binomial generalized estimating equation models for the herd positivity‐human TST positivity association, stratified on sex; and for the raw milk consumption‐human TST positivity association, stratified on frequency of milk consumption. Having at least one TST‐positive bovid in the household's herd was significantly associated with lower risk of TB among men (prevalence ratio [PR] 0.66, 95% CI 0.49, 0.87) but was not significantly associated with TB among women (PR 1.21, 95% CI 0.76, 1.95). This apparent protective effect was contrary to our a priori hypothesis of higher exposure effect among men, the primary caretakers of cattle. This finding may be the result of residual confounding by socioeconomic status; wealthier individuals may be less likely to be TB positive, but more likely to have TST‐positive herds by virtue of larger herd sizes, ability to purchase new and possibly infected stock, and propensity to keep more TB‐susceptible European breeds. For raw milk consumption, effect estimates were close to one and not statistically significant. Thus, in settings where bovine TB prevalence is low, such as Uganda, cattle‐associated zoonotic transmission may be rare, and cattle‐associated risk factors may not be important drivers of human TB burden. 相似文献