Evaluation of cytokine messenger RNA expression in peripheral blood mononuclear cells from dogs with canine demodicosis     

Tani K  Morimoto M  Hayashi T  Inokuma H  Ohnishi T  Hayashiya S  Nomura T  Une S  Nakaichi M  Taura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):513-518

Using RT-PCR and semi-quantitative PCR, mRNA expression for canine interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5, IL-10, tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta in peripheral blood mononuclear cells (PBMCs) was examined in dogs with or without demodicosis. mRNA expression for IFN-gamma as well as TNF-alpha in dogs with demodicosis (localized (LD) and generalized (GD)) was slightly lower than those in dogs without demodicosis (healthy controls). Expression of IL-5 mRNA in dogs with demodicosis was higher than that in control dogs, but there were no significant differences in IL-4 and IL-10 mRNA expression levels among the three groups. On the other hand, expression levels of TGF-beta mRNA in dogs with GD were higher than those in control dogs and dogs with LD. The expression levels of IL-5 and TGF-beta mRNA decreased in all three dogs with GD which showed resolution of the clinical signs. Taken together, these results suggest that the Th2-like response in PBMCs from dogs with demodicosis is up-regulated, and that subsequent increased expression of IL-5 and TGF-beta mRNA in dogs with GD is reversible after treatment. Therefore, these cytokines, particularly IL-5, might be a useful clinical index of the clinical course in demodicosis. Also, increased TGF-beta mRNA expression might be a key factor for revealing the difference in the mechanism of onset between LD and GD.  相似文献   

Expression of T helper 1 and T helper 2 cytokine mRNAs in freshly isolated peripheral blood mononuclear cells from dogs with atopic dermatitis     

Hayashiya S  Tani K  Morimoto M  Hayashi T  Hayasaki M  Nomura T  Une S  Nakaichi M  Taura Y 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2002,49(1):27-31

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Deformation behavior of wood under stress relaxation in a radial direction as a laminated annual-ring structure     

Kosei?AndoEmail author  Mami?Morimoto  Yoshitaka?Kubojima 《Journal of Wood Science》2004,50(4):381-382

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Seasonal changes in proteolytic enzymes of yellowtail Seriola quinqueradiata (Temminck & Schlegel; Carangidae) fed extruded diets containing different protein and energy levels     

Patricia Yumi Morimoto Kofuji  Atsushi Akimoto  Hidetsuyo Hosokawa  & Toshiro Masumoto 《Aquaculture Research》2005,36(7):696-703

Seasonal variations in pepsin, trypsin and chymotrypsin activities and protein digestibility were studied in yellowtails (Seriola quinqueradiata) reared for 1 year with extruded diets containing different levels of protein. Trypsin and chymotrypsin storage levels in the digestive tissues of starved fish were affected by seasonal changes in water temperature. Actual digestion activities of trypsin and chymotrypsin were low at lower water temperatures, but pepsin activity in the stomach tissue was not affected by low temperatures. On the other hand, trypsin and chymotrypsin activities in the intestinal contents were higher during lower water temperature months, while pepsin activity in the stomach contents was low at lower water temperatures. Apparent protein digestibility (APD) did not differ among the dietary treatments in the higher water temperature months, while in colder months it was higher in fish fed diet 1 than in fish fed diets 2 and 3. The APD values reflected pepsin activity in the stomach contents in all sampling months. Therefore, lower APD in colder months seems to be attributed to lower protease activity in the gastric digesta, implying that enhanced pepsin secretion from the stomach tissue might improve protein digestibility and growth performance in yellowtails during winter.  相似文献   

Trypsin restoration time in the pyloric ceca of yellowtail <Emphasis Type="Italic">Seriola quinqueradiata</Emphasis>     

Koji?Murashita  Satoshi?Kubota  Patricia?Yumi?Morimoto Kofuji  Hidetsuyo?Hosokawa  Toshiro?MasumotoEmail author 《Fisheries Science》2005,71(6):1274-1279

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Perinatal changes in the population of duodenal goblet cells in the rat     

M Morimoto  Y Morikawa  T Okada 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(2):573-575

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Expression and subcellular localization of GSE protein in germ cells and preimplantation embryos     

Mizuno S  Sono Y  Matsuoka T  Matsumoto K  Saeki K  Hosoi Y  Fukuda A  Morimoto Y  Iritani A 《The Journal of reproduction and development》2006,52(3):429-438

We previously identified a novel gonad-specific expression gene (Gse) and investigated its expression during gametogenesis in the mouse testis and ovary. In this study, we generated a polyclonal antibody to GSE protein and determined the profiles of the protein's expression in germ cells and preimplantation embryos in detail using immunocytochemical and immunofluorescence staining. In a Western blot analysis, the anti-GSE antibody recognized long and short isoforms (approximately 27.6 kDa and 23.1 kDa) of the protein in the mouse testis and the long isoform in the ovary. In the mouse testis, GSE protein was expressed in spermatocytes I in the pachytene stage, round spermatids, and elongated spermatids. In the mouse ovary, the protein was located in the cytoplasm and nucleus of all oocytes regardless of the stage of the ovarian follicles. In preimplantation embryos from the pronuclear to blastocyst stage, however, GSE protein was mainly detected in the nuclei of cells. At the blastocyst stage, the protein was confirmed to have accumulated in the inner cell mass (ICM), whereas it had mostly disappeared from the trophectoderm (TE). These findings suggest that GSE protein may play a role in the establishment of nuclear totipotency and may be associated with early lineage specification.  相似文献   

Surgical and radiotherapy treatment of a spinal cord ependymoma in a dog     

Ueno H  Morimoto M  Kobayashi Y  Hizume T  Murayama N  Uzuka Y 《Australian veterinary journal》2006,84(1-2):36-39

A 4-year-old Beagle dog was presented for investigation of a left pelvic limb gait abnormality. Neurolocalisation indicated a lumbar (L2 to L5) spinal cord lesion. On magnetic resonance imaging (MRI), an intramedullary mass was demonstrated at L3. The mass was partially removed under general anaesthesia and a diagnosis of ependymoma was made on histological examination. The dog was treated with postoperative orthovoltage x-ray radiation (total dose; 44 Gy given in 11 fractions over a 4 week period) combined with low dose carboplatin (25 mg/m2). The dog was alive 16 months after surgery without further neurological deficits. No further tumour growth was detected on subsequent MRI evaluations.  相似文献   

Evaluation of the biliary and brain distribution of technetium Tc 99m sestamibi in healthy dogs with the ABCB1 wildtype genotype before and after treatment with spinosad     

MacKay CS  Mattoon JS  Roberts GD  Tucker RL  Morimoto TR  Mealey KL 《American journal of veterinary research》2012,73(6):814-820

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Calreticulin expression in neoplastic versus normal dog mammary glands: a cDNA subtraction-based study     

Okawa T  Kurio Y  Morimoto M  Hayashi T  Nakagawa T  Sasaki N  Okuda M  Mizuno T 《Research in veterinary science》2012,92(1):80-91

This is the first report describing the expression of canine calreticulin (cCRT) in canine mammary gland tumour (MGT). Using cDNA subtraction method, it is found that mRNAs of CRT, cathepsin A, ovostatin, and lactotransferrin were differentially expressed in mammary adenocarcinoma as compared to hyperplasia, both of which were obtained from the dog. Furthermore, the mRNA expression levels of CRT and cathepsin A were significantly higher in canine MGT samples than in nontumour samples. In contrast, immunohistochemical studies have indicated that the expression of cCRT protein found to be detected in most of mammary gland tissues and was not correlated to the types of canine MGTs. Furthermore, cCRT was molecularly cloned, and the amino acid sequence of cCRT was found to be very similar to those of other species. Further studies are required to elucidate additional roles of cCRT in canine MGT.  相似文献