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1.
Nutrient availability in the soil is not only determined by chemical, physical, and micro-biological factors but can be actively influenced by the plant root system. Various components of root activity have been studied in our laboratory during the last 10 years. The major findings are summarized and discussed with special emphasis on root morphology, cation-anion imbalance and proton release, the mechanism of proton release and anion secretion. Large species differences in root morphology were found between red clover and rye-grass. Root morphology was also found to be controlled by environmental and physiological factors. Experimental results obtained with different plant species suggest that proton release by plant roots is not primarily a consequence of excess cation uptake to achieve charge balance in the plant, but rather that active proton extrusion by ATPase activity creates a charge imbalance and a pH gradient which drive nutrient uptake. The pH gradient across the plasmalemma of root cells was also found to be essential for the retention of organic anions by plant roots.  相似文献   
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Oxygen consumption, ammonia excretion and fish swimming speed were measured in fish induced to swim by optomotor reaction in a circular metabolism chamber. The relationship between the swimming speed and fish metabolism described by exponential equations allowed the extrapolation to the standard metabolism, i.e. at zero swimming speed. The partitioning of the catabolised protein in the energy supply was estimated based on AQ (volume of ammonia/ volume of oxygen) values. Weight specific standard metabolism, as expressed by the ammonia excretion rate, decreased by one order of magnitude in coregonids as the fish grew from 20 to 780 mg body weight. The slope of the relationship between oxygen uptake and swimming speed decreased in coregonid ontogenesis. In salmon, after 12 days of fasting 28% of energy used was derived from protein, whilst coregonid juveniles utilized mostly lipid. Active swimming in fasted juveniles of coregonid, as well as in salmon, led to the accelerated utilization of protein as a source of energy, based on AQ coefficients. In juveniles acclimated to a range of water temperatures from 14 to 26°C, the changes in standard or active metabolic rate (expressed as oxygen uptake or ammonia excretion) were described by Q10 coefficients. They were generally higher for the ammonia excretion rate than for the oxygen uptake rate and for active metabolism than for standard metabolism. Utilization of protein as energy for swimming differed significantly between the species, being in general one order of magnitude higher in coregonids than in salmon. The use of protein for swimming activity tended to decrease during coregonid ontogenesis.  相似文献   
3.
A study was conducted to evaluate tissue storage and mobilisation of L-ascorbic acid (AA) in the Siberian sturgeon (Acipenser baeri) fed three different experimental diets. The three treatments consisted of a diet devoid of vitamin C (diet A0) and two diets supplemented with equivalent of 300 mg AA kg–1 in the form of either silicone-coated ascorbic acid (diet SC) or of ascorbyl-2-polyphosphate (diet AP). During the first phase (4 months) of the trial, six batches of 130 Siberian sturgeon (initial body weight: 25.5±0.5 g) each were fed one of the three diets in duplicate. During the second phase (3 months), fish from groups SC and AP were switched to diet A0 and those fed diet A0 during the first phase were switched to diet SC. Irrespective of the dietary treatment, growth rates were not significantly different from each other. At the end of phase I, in all tissues studied, total ascorbic acid (TAA) concentrations were higher in Siberian sturgeon fed diet AP than in the other two groups. During phase II, tissue ascorbate depletion was also higher in the AP group than in the other two groups. Transfer of the AA-free diet fed group onto a diet supplemented with 300 mg AA kg–1 (diet SC) led to a slight increase in the TAA concentrations in all tissues. Blood plasma tyrosine concentrations were not significantly different between the three groups. Whole-body collagen levels were affected by dietary AA levels or forms at the end of phase I; the differences were not significant at the end of phase II. Muscle collagen levels were slightly affected. L-Gulonolactone oxidase activity was found in the kidney of Siberian sturgeon, but not in the liver. The ascorbyl-2-polyphosphate appears to be either better utilised by Siberian sturgeon, like in many other teleosts, or more stable than the silicone-coated AA during food processing and storage. Presence of L-gulonolactone oxidase activity in Siberian sturgeon kidney combined with the absence of gross scorbutic signs in AA-free diet fed groups expressing very good growth rates suggested no need of dietary AA byA. baeri.  相似文献   
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Diets based on fish meal and soyabean meal were fed to grass carp fry, the average body weight of each individual being 0.4 g. During the 70 days of the experiment, the best results were obtained with a diet containing 40% fish meal, when growth increment, protein efficiency ratio and apparent net protein utilization amounted to 209, 1.26 and 20.3, respectively. There was a deficiency in sulphuric amino acids in both fish meal- and soyabean meal-containing diets, and this was particularly marked in the latter. A significant decrease in available lysine was found in the diets containing soyabean meal. The main reason for the retardation in fish growth and the reduction of protein utilization when soyabean meal content is increased in the diets, seemed to be that the destruction of anti-nutritional factors by the heat processing of the meal was only partial.  相似文献   
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Abstract.— Despite a large economical value of the ornamental fish trade, little information is available on the dietary ascorbic acid requirements of these species. Ascorbic acid requirements of fishes of the cichlid family appear to vary widely. Juvenile angelfish, a widely produced ornamental cichlid, were maintained on diets containing graded levels of ascorbyl monophosphate. Liver ascorbic acid concentrations after 96 d of feeding were significantly reduced in groups receiving 120 mg or less ascorbic acid equivalents/kg diet. However, no differences in growth or mortality between groups were found, and no external signs of ascorbic acid deficiency were observed, indicating a high resistance of this species against prolonged ascorbic acid deficiency. Based on the long possible life span of angelfish in the aquarium, we proposed a conservative dietary ascorbic acid requirement of 360 mg/ kg diet, necessary to maintain maximum tissue storage of this vitamin.  相似文献   
9.
The involvement of photoperiod and temperature in the regulation of reproductive processes was investigated in female yellow perch. Initially, all fish kept indoors were exposed to the same water temperature (22°C) and photoperiod (15L:9D). By the end of August, following the first sampling, fish were submitted to different photothermal regimes. Group A3 was maintained under photothermal conditions characteristic for southern Ohio. Group B3 was submitted to a condensed light/temperature regime designed to accelerate photothermal changes. The mean gonadosomatic index in group A3 gradually increased throughout the experiment. Gonadosomatic index in group A3 was higher than that in group B3 from February through April. The highest plasma concentrations of estradiol in group A3 occurred in November and December and exceeded those in group B3. Plasma concentrations of testosterone in group A3 were highest in December and tended to be higher in October and April than in other months. There was no difference in plasma testosterone between groups A3 and B3 except in March when concentrations of this androgen were extremely high in group B3. Ovulation occurred earlier in B regime fish in comparison to that of A regime fish. However, egg quality of fish from regime B was lower than that of fish from regime A. We conclude that the condensed photothermal cycle is not an entirely effective method of inducing out-of-season spawning in female yellow perch. These data suggest that although the compression of the photothermal cycle slightly accelerated spawning, it also caused disturbances in patterns and levels of plasma steroids, diminished ovarian and hepatic growth, and reduced the quality of eggs. Thus, temperature and/or photoperiod may have only a modifying effect on the onset of perch spawning, but these environmental cues seem to markedly influence earlier stages of gonadal growth and development.  相似文献   
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