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排序方式: 共有596条查询结果,搜索用时 31 毫秒
1.
用假阴道采集10只新西兰白兔的精液,经测定精子活力强,密度大,呈前进运动。然后用三羟甲基氨基甲烷缓冲液作抗冻保护,使精液在2小时内冷却至5℃,放置2天。低温保存时加入5毫克分子浓度的咖啡碱或茶碱,并在37℃条件下持温2小时。在受胎率试验中,选用43只新西兰白兔,每次用新鲜精液,低温冷藏精液或添加5毫克分子浓度茶碱于稀释低温保存精液输精0.5毫升(含精子15x10~6)。结果表明,含5毫克分子浓度咖啡碱或茶碱稀释的低温保存精液,游动精子的比例增加(P<0.01),但持温保存时游动精子的比例降低(P<0.01),且精子顶体异常率增加(P<0.01),乳酸脱氢酶释放进入细胞间质。采用含5毫克分子浓度茶碱低温保存精液的配种效果与鲜精相同;但鲜精与冷藏精液相比,则差异显著(P<0.05)。采用鲜精,冷藏精液及含茶碱低温保存精液输精,母兔的产仔率分别为70.6%,41.7%和64.3%. 相似文献
2.
Bussanich MN Rootman J Kumi C Gudauskas G 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(9):263-266
The ocular absorption, elimination and toxicity of 12.5 mg of methotrexate in 0.5 cc administered subconjunctivally was studied in the dog. The purpose of this study was to establish ocular pharmacokinetics for treatment of intraocular tumours in dogs. Drug levels were measured in ocular compartments, and serum at 1,2,4,6,8 and 12 hours respectively. Tumoricidal concentrations of the drug were attained in the anterior chambers of the injected eyes up to and including six hours postinjection. Consistent signs of toxicity were anorexia, vomiting, weight loss and leukopenia. Local ocular toxicity following subconjunctival injection was minimal conjunctival hyperemia lasting up to 48 hours. 相似文献
3.
Raskin RE Nipper MN 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1992,21(2):62-67
Frozen sections and imprint smears were used to evaluate the presence and pattern of cytochemical staining reactions in the B- and T-cell regions of lymph nodes from normal dogs and dogs with lymphoma. Staining procedures evaluated included peroxidase (PER), Sudan black B (SBB), naphthol AS-D chloroacetate esterase (CAE), alpha-naphthyl butyrate esterase (NBE), acid phosphatase (ACP), and leukocyte alkaline phosphatase (LAP). In normal lymph nodes, macrophages and some lymphocytes within the interfollicular (T-cell) region and medulla stained positive with ACP and NBE. Smaller numbers of macrophages also occurred sporadically within the germinal follicles. Cells positive for PER, SBB, and CAE were scattered infrequently throughout all regions of the normal lymph node, consistent with granulocytes and mast cells. The LAP stained cells were predominantly and prominently located within the mantle zone of secondary follicles and to a much lesser extent within the germinal centers, compatible with B-cell lymphocytes derived from follicular center cells. Of the 12 dogs with lymphoma, 7 cases (4 immunoblastic, 2 large noncleaved, 1 small noncleaved) stained diffusely positive with LAP, 4 cases (all lymphoblastic) had numerous focally positive lymphocytes using ACP and NBE, and 1 case (immunoblastic) did not stain positive with any of the cytochemical reactions. Cytochemical staining of canine lymph nodes with NBE, ACP, and LAP proved useful in distinguishing between B- or T-cell regions and detecting different cell types of canine lymphoma. 相似文献
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Koutinas AF Saridomichelakis MN Argyroudis S Koutinas CK Karatzanos P Giadinis N 《Veterinary dermatology》2007,18(4):211-216
In a flock of 290 sheep, a total of 20 ewes (6.9%) with an age range of 1.5-4 years, developed a nonpruritic dermatitis characterized by alopecia/hypotrichosis, erythema, hyperpigmentation, crusting, superficial ulcers, exudation and thickening of the skin that was localized mainly to the peri-ocular area. Frequent and massive feeding of swarming sucking flies on the lesional sites was witnessed. In every instance (n = 6), Staphylococcus aureus was cultured from the exudate and the aseptically obtained skin biopsy samples from both recurrent and new cases. The main predisposing factor was the presence of small troughs, which forced the sheep to compete for food. Intramuscular administration of cefalexin for 7 days resulted in the resolution of lesions over a 2-week period. No further cases or recurrences were seen over the 16-month follow-up period. The main histopathological feature of this skin disease was a superficial and deep, perivascular to interstitial eosinophilic and mononuclear dermatitis. 相似文献
8.
The repellent effects of the essential oil of Lavendula angustifolia on adults of Hyalomma marginatum rufipes was studied at concentrations of 5, 10 and 20% v/v. A suitable tick climbing bioassay based on the questing behaviour of ticks was used to test for repellency. High percentage repellency (range 70-100) was shown at all concentrations of the essential oil of L. angustifolia, although at 5% v/v it only persisted for the first 40 minutes compared with 120 minutes at other concentrations (10 and 20% v/v). The repellent strength of L. angustifolia compared well (P > 0.05) with that of DEET (N,N-diethyl-m-toluamide), a commercial reference repellent, for the 2-hour period of the study. 相似文献
9.
A single-step duplex polymerase chain reaction (PCR) technique and traditional microscopic examination of haemolymph smears were used to detect Babesia bigemina and/or Babesia bovis infection in engorged female ticks of Boophilus microplus recovered from calves raised in an endemic area of the State of Minas Gerais, Brazil. In the PCR amplification of tick-derived DNA, pairs of oligonucleotide primers specific for a 278-bp sequence from B. bigemina and for a 350-bp sequence from B. bovis were used conjointly. The microscopic examination of haemolymph revealed that 16.7% of the engorged ticks were infected with Babesia spp., although no significant differences (rho > 0.05) were found in the infection rate of ticks collected from calves of different age groups. PCR analysis showed that 77.8% of the engorged ticks whose haemolymph contained sporokinetes were infected with B. bigemina, 7.8% with B. bovis and 14.4% with both protozoan species. However, the PCR assay further revealed that, amongst the engorged female ticks whose haemolymph was apparently negative for the presence of sporokinetes, 15.6% were infected with B. bigemina, 2.2% with B. bovis and 10.0% with both species. The duplex PCR method is thus more efficient and sensitive than the microscopic assay and also permits facile identification of the protozoa species present in engorged female ticks. 相似文献
10.
从果实经济性状、生长结果习性、物候期等方面观察记载了青(木奈)在铜仁市的引种表现.结果表明,青(木奈)能适应引进地的生态条件,表现良好.在主要栽培技术上作了探索,使结果初期的产量达到了较高水平. 相似文献