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Quantities and types of ceramides and their relationships to physical properties of the horn covering the claws of clinically normal cows and cows with subclinical laminitis 
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下载免费PDF全文 H. Higuchi M. Nakamura A. Kuwano M. Kasamatsu H. Nagahata 《Canadian journal of veterinary research》2005,69(2):155-158
Quantities and types of ceramides and their relationships to physical properties of the horn covering the claws of clinically normal cows and cows with subclinical laminitis were investigated. Total ceramide content of the horn covering the sole and wall from cows with subclinical laminitis was 872.2 +/- 146.6 microg/g and 528.6 +/- 61.3 microg/g, respectively, and was significantly (P < 0.01, 0.05) lower than that from clinically normal cows. The mean moisture content in the claws from cows with subclinical laminitis (43.5% +/- 4.3%) was significantly (P < 0.05) higher than that in the claws from clinically normal cows. The hardness of claws from cows with subclinical laminitis (35.2 +/- 3.5) was significantly (P < 0.05) less than that of claws from clinically normal cows. Significant correlations between ceramides and moisture content (P < 0.001) and between ceramide and hardness (P < 0.001) were found in clinically normal cows and cows with subclinical laminitis. Our results indicate that decreases in ceramide contents may be related to changes in physical properties of the horn covering the claw in cows with subclinical laminitis. 相似文献
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Yin SA Makara M Pan Y Ishiguro H Ikeda M Numakunai S Goryo M Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):599-606
Thirty-three cases of enzootic bovine leukosis (EBL) and 14 cases of sporadic bovine leukosis (SBL) were examined by immunohistochemistry using 6 monoclonal antibodies against leukocyte differentiation molecules of bovine leukocytes. There were 17 cases of B-1a cell type, 10 cases of B-1b cell type and 6 cases of B-2 cell type in EBL, and 5 cases originating from B cells (B-2 cell type) and 9 cases originating from immature T cells in SBL. The average age for the EBL cases of B-1a cell type was 8.6 years, B-1b cell type was 6.5 years, and of B-2 cell type was 4.5 years. In cases of SBL, immature T cell type patients were younger than B-2 cell type ones. The lymphoma originating from B cells differed from that originating from T cells in morphology. In T cell tumors, the nucleus of tumor cells was round, the edge of the cytoplasm obvious, and tumor cells were sporadically present and proliferated. When compared with T cells, the region among B cells was obscure. But, there was no relation between phenotype and the histologic classification of tumor cells. In EBL, beyond the lymph node, tumors of B-1a and B-1b types had developed in the heart and abomasum, and those of the B-2 type tended to occur in liver. In SBL, B-2 type and T type cells formed tumors in the liver, kidney, thymus, and one case of T-cell type tumor formed on the skin. We would like to propose a new classification of bovine leukosis as EBL, calf type B-cell lymphoma, juvenile T-cell lymphoma and skin type T-cell lymphoma. 相似文献
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Manami Inaba Takeshi Kimura Rika Kikukawa Mitsuko Iwasaki Masato Nose Satoru Suzuki 《Fisheries Science》2007,73(3):615-622
ABSTRACT: Seasonal changes in the infection state of marine birnavirus (MABV) in Japanese flounder Paralichthys olivaceus and in rearing sea water are described. Sea water and 10–11 healthy fish were sampled monthly from April 2002 to February 2003. The MABV genome was detected throughout the year in > 80% of the fish examined at each sampling. The virus was isolated from the liver, kidney, and spleen, but not from the brain. The detection rate in each organ increased from April to October, and then decreased. Detection of virus antigen by the indirect fluorescent antibody technique also showed that the virus was present from spring to autumn (June–September) in the liver, kidney, and spleen, but not the brain. Sequence analysis of the MABV genome at the VP2–NS region revealed two specific mutations compared to the standard yellowtail strain (Y-6). It is suggested that the infection state of MABV in Japanese flounder changes to a latent or persistent infection after autumn. MABV was detected in sea water between September and February, suggesting that virus particles in the environment are relatively higher during cool seasons. 相似文献
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Fujio Kasamatsu Paul Ensor Gerald G. Joyce & Noritugu Kimura 《Fisheries Oceanography》2000,9(3):214-223
The relationship between the distribution of minke whales ( Balaenoptera acutorostrata ) in the Bellingshausen and Amundsen Seas (longitude between 60°W and 120°W), and environmental and physiographic variables (sea-surface temperature, sea-ice extension, and sea-floor-slope type), was studied to determine whether these environmental and physical factors affect the distribution and density of minke whales. The analysis was based on sightings data obtained from the 1989/90 and 1982/83 IWC/IDCR cruises. The mean sea-surface temperatures for comparable areas were significantly higher in 1989/90 (2.04°C) than in 1982/83 (1.12°C), and the area where the sea-surface temperature was greater than 1°C in the 1989/90 study was approximately twice that of the 1982/83 study. Additionally, during the surveys, the extent of the sea ice in 1989/90 was less than that in 1982/83, with the mean ice edge about 92.6 km (50 nautical miles; 1 nautical mile ≈1.852 km) farther south in 1989/90 than in 1982/83. This is consistent with the sea ice extent observed in winter, when the sea ice extent was less in 1989 than in 1982. The distribution of minke whales was substantially different between the two surveys, with the density and abundance of minke whales being greater in 1982/83 than in 1989/90. The warmer sea-surface temperatures, fewer cold-water intrusions, and the smaller extent of sea ice in 1989/90 may be related to the difference in distribution of minke whales from 1982/83, possibly owing to the shift in availability of prey. 相似文献
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Monobe M Ema K Kato F Hirokane H Maeda-Yamamoto M 《Journal of agricultural and food chemistry》2007,55(7):2543-2547
A technique for screening immune-enhancing polysaccharides in food using the phagocytotic activity of 1,25-dihydroxyvitamin D3 (VD3)-differentiated HL60 cells is presented. HL60 cells, a human acute promyelocytic cell line, can differentiate along the monocytic lineage following exposure to VD3 or phorbol-12-myristate-13-acetate (PMA). For differentiated cells along the monocytic pathway, HL60 cells were maintained in RPMI 1640 medium supplemented with 10% FBS and 120 nM VD3 for more than 1 week. VD3-differentiated HL60 cells were seeded into 48-well plates, YG-labeled microspheres and polysaccharides were added and mixed using a plate shaker at 1100 rpm for 30 s, and then the mixture was incubated overnight at 37 degrees C in 5% CO2. The cells were fixed with 2% formaldehyde and resuspended in phosphate-buffered saline. The rate of phagocytosis was measured with a flow cytometer. VD3-differentiated cells but not non- and PMA-differentiated cells resulted in an elevation of phagocytic activity by various immune-enhancing polysaccharides in foods. 相似文献
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High level expression of biologically active canine interferon-alpha subtype 4 using a baculovirus 总被引：1，自引：0，他引：1
Ruttanapumma R Anzai M Takegawa M Okamura M Maehara N Sato K Yoshioka K Itoh A Nakamura M Takehara K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1347-1349
In this study, a high amount of bioactive recombinant canine interferon-alpha subtype 4 (CaIFN-alpha4) was expressed in a baculovirus system. For easy purification, it was expressed as a CaIFN-alpha4 bearing histidine hexamer at the C-terminal region, designated CaIFN-alpha4His. CaIFN-alpha4His was detected in culture supernatants of insect cells infected with the recombinant virus using sodium dodecyl sulfate-polyarcylamide gel electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue staining. The level of expression was very high, and approximately 1 mg of purified protein, with 5.0 x 10(7) units/mg, was obtained from 300 ml of culture supernatant. The purified product showed antiviral activity against Vesicular stomatitis virus on canine tumor cell line A72 and chicken embryo fibroblast cells. 相似文献
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Fujino T Matsuo T Okada M Matsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1191-1193
Detection rates from the samples including a small number of Cryptosporidium parvum oocysts were compared between the sugar flotation and the sugar centrifugal flotation methods. As the results, the oocysts were detected from 70 and 80 of 100 samples including 6.0x10(2) and 1.0x10(3) oocysts per 1 ml by the flotation method, respectively, whereas from 52 and 53 of the same samples by the centrifugal flotation method. Therefore, it was considered that the flotation method is the most suitable method for the detection from samples including a small number of Cryptosporidium oocysts. It is also suggested that results of the sugar flotation method were reliable for samples including more than 1.0x10(3) oocysts/ml. 相似文献
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Shimokawa T Doihara T Makara M Miyawaki K Nabeka H Wakisaka H Kobayashi N Matsuda S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(2):155-160
The stomach of the Pacific white-sided dolphin is divided into three parts: forestomach, proper gastric gland portion, and pyloric chamber. The histological features of the dolphin stomach are similar to those of terrestrial mammal stomachs, although the distribution of glycoconjugates in mucosal cells of the dolphin stomach is unknown. To learn about glycoconjugates in cetacean gastric mucosa, the glycoconjugate distribution in the mucous epithelium of the Pacific white-sided dolphin was studied using 21 lectins. Among the lectins tested, GSL-I and DBA specifically labelled the superficial layer of the forestomach epithelium. GSL-I, SBA, RCA-I, VVA, GSL-II, DSL, LEL, STL, s-WGA, WGA, PNA, and Jacalin labelled the luminal surface of the chief cells in the proper gastric gland. GSL-I, SBA, RCA-I, DSL, LEL, STL, s-WGA, PNA, and LCA labelled tubular structures in the cytoplasm of parietal cells. The surface portion of the pits in the pyloric chamber strongly reacted with RCA-I, GSL-II, WGA, PNA, LCA, PHA-L, and UEA-I, whereas the neck portion reacted weakly. Although lining one tubular portion, individual secretory cells in the pyloric gland displayed a heterogeneous reaction. This is the first report on the lectin histochemistry of a cetacean stomach and reveals GSL-I and DBA as specific marker lectins for the cornified stratified squamous epithelium cells of the Pacific white-sided dolphin. The stomachs of cetaceans and terrestrial mammals have similar histological features and mucous glycoconjugate content. 相似文献