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1.
Summary The possibility of using proline accumulation and fluorescence inhibition as predictive tests for drought tolerance in durum wheat has been investigated. The drought susceptibility of 25 genotypes was evaluated by comparing yields and yield components in irrigated and non irrigated conditions in the field. A drought susceptibility index (DSI) was calculated based on yields from irrigated and dry treatments and compared with the results obtained using the two physiological criteria. Proline accumulation and chlorophyll fluorescence inhibition were found to be significantly and negatively correlated with DSI of grain yield, biological yield, and thousand kernel weight, and tiller index. The use of both criteria for breeding durum wheat in Mediterranean dryland is discussed. 相似文献
2.
Mario A. Pagnotta Alfredo Impiglia Oronzo A. Tanzarella Miloudi M. Nachit Enrico Porceddu 《Genetic Resources and Crop Evolution》2005,51(8):863-869
The durum wheat landrace Haurani (Triticum durum Desf.) is grown under contrasting climatic regions of Syria from Deir Ezzor, in the North-East (230 m altitude, 150 mm mean annual rainfall), to Qunaytra, in the South-West (1060 m altitude, 825 mm mean annual rainfall). In order to assess the genetic variation between and within Haurani populations, samples from eight provinces of Syria (Daraa, Damascus, Qunaytra, Deir Ezzor, Hassakeh, Aleppo, Homs and Hama) were analysed by RFLPs and seed storage proteins of glutenin subunits as markers. The analyses showed the presence of genetic polymorphisms in all populations with the highest values in those from Homs and Hassakeh. Moreover, the results point out differences in genetic distances between populations; some populations were further apart, such as Damascus and Aleppo, whereas others were closer to each other, for instance Homs and Hama. Cluster analysis identified two distinct groups of populations, characterized by geographical proximity, with similar rainfall and altitude. It is suggested that the similarity of landraces at locations close to each other might be the result of more frequent seed exchanges between farmers or of gene flow due to 5% estimated outcross rate of Haurani. 相似文献
3.
The total content of flavonol glycosides was lowest in P. avium L., medium in P. cerasus L. and highest in P. fruticosa Pall. The hybrids between P. fruticosa × P. avium and P. cerasus × P. fruticosa showed an intermediate pattern. Quercetin glucoside and quercetin rhamnoglucoside rutin) occurred regularly in all trees investigated. Some flavonol glycosides could not be identified exactly.Young shoot segments of P. avium (F12/1) were grown for 4 weeks in vitro in continuous darkness or at a day length of 16 hours. The basal medium was supplemented with several flavonoids. Rutin and dihydroquercetin stimulated the callus growth. The tissue was unresponsive to quercetin and kaempferol. Among the factors contributing to the callus production were the light regime as well as the growth factors of the medium. 相似文献
4.
Jemanesh K. Haile Karl Hammer Ayele Badebo Miloudi M. Nachit Marion S. Röder 《Genetic Resources and Crop Evolution》2013,60(2):513-527
A set of 77 markers was used to describe the genetic diversity in a group of 58 tetraploid wheat accessions. Analysis was performed using 31 neutral SSR markers, 31 SSR/STS markers linked with reported major stem rust resistance genes and 15 SSR markers linked with QTL identified for resistance to Ethiopian stem rust races of Puccinia graminis Pers. f. sp. tritici Eriks. et E. Henn. (Pgt),including Ug99. The material consisted of 32 (Triticum durum s.l. incl. T. aethiopicum Jakubz., Triticum turgidum and Triticum polonicum) landraces and 22 registered T. durum varieties from Ethiopia that were released 1966–2009 and four T. durum varieties from ICARDA. A total of 720 alleles were detected. Considering the three marker sets, the mean number of alleles was higher for major stem rust resistance gene linked markers (9.9) followed by neutral SSR markers (9.2) and markers linked with QTL for stem rust resistance (8.5). Dendrograms derived from UPGMA analysis grouped the accessions into two major clusters. The principal component analysis based on the combination of the three marker sets formed three groups. The 1st group was composed of all the improved varieties, whereas the 2nd and the 3rd group contained the landraces. All the landraces that formed the 3rd group were susceptible to Ethiopian stem rust races of Pgt including Ug99. The information on the extent of the genetic diversity of the improved varieties obtained in this investigation will be helpful for developing appropriate breeding strategies to broadening the genetic base of durum wheat varieties in further breeding programmes. 相似文献
5.
Among the most important Mediterranean annual crops durum wheat is widely grown in drought-prone areas. Therefore improving water-use efficiency 《分子植物育种》2007,5(2):223-223
Among the most important Mediterranean annual crops, durum wheat is widely grown in drought-prone areas. Therefore, improving water-use efficiency (WUE) of durum wheat represents a major breeding goal. IDu-WUE (Improving Durum wheat for Water Use Efficiency and yield stability through physiological and molecular approaches) is a collaborative project among public and private research centres in Italy, Spain and WANA (West Asia and North Africa) countries (Morocco, Tunisia, Syria and Lebanon) funded by the European Union aimed at investigating the genetic variation for WUE and yield stability in durum wheat grown in Mediterranean droughtprone areas. During the first year of the project, a number of morpho-physiological traits (e.g. early vigour, flowering time, leaf rolling, number of fertile tillers, etc.), WUE, WUE-related traits (e.g. carbon isotope discrimination, canopy temperature, chlorophyll fluorescence, etc.), yield and its components have been investigated in a RIL population (249 lines) and a collection of ca. 190 durum wheat accessions characterized by a high level of linkage disequilibrium (Maccaferri et al., 2005), 相似文献
6.
Summary Nineteen durum wheat landraces, cultivars or advanced lines of different origins in West Asia and North Africa (WANA), and three barley and two bread wheat varieties were evaluated for their boron (B) toxicity tolerance. Seedlings were grown at five levels of soluble soil B in a plastic house under controlled temperatures. Significant differences existed between the durum wheat entries in days-to-symptom appearance and foliar symptom score. Under the highest soil B treatment, large differences existed between entries for dry weight per plant (P<0.05) but differences were non-significant for shoot B concentrations. Days-to-symptom appearance was highly correlated with symptom score, which was not correlated with shoot B concentrations. Boron toxicity symptom scores of the durum wheat entries ranged from the sensitive barley check to the moderately sensitive bread wheat check. As expected, days-to-symptom appearance decreased and symptom severity increased as the soil B concentrations increased.The result of this study supported the preliminary finding that small, though statistically significant, variation in B toxicity symptom scores exist in durum wheat. The higher CV of symptom scores found here was mainly due to one sensitive entry, Cakmak. If Cakmak was excluded from the analysis, the CV would be reduced by half, to 10%. Durum wheat genotypes which are more tolerant to B toxicity should be sought. Based on the results of this study, and of soil surveys and information collected in WANA, germplasm collected from Algeria, Iraq, Libya, Syria, and the Anatolian Plateau of Turkey should be screened first.Abbreviations B
boron
- WANA
West Asia and North Africa 相似文献
7.
Near-infrared Reflectance Spectroscopy (NIRS) is widely used for routine, non-destructive assessment of grain quality in cereals. In this work, we assess the use of NIRS as an indirect indicator of grain yield (GY) in durum wheat grown in Mediterranean conditions. Plants were grown under rain-fed conditions in two sites in NW Syria, Breda and Tel Hadya, and a third trial was planted at Tel Hadya under support irrigation. We measured the spectral reflectance signature between 1100 and 2500 nm in flour from the same grains harvested for yield determination. By Partial Least Squares (PLSR), we obtained a model of the association between agronomic determinations of GY and these spectra. We found significant (p < 0.001) relationships between GY and NIRS-predicted values, with r
2= 0.36 (N= 96), r
2= 0.72 (N= 72) and r
2= 0.52 (N= 138) in Breda, and Tel Hadya rainfed and irrigated, respectively. Full cross validation of these models gave r
2 between 0.31 and 0.33 (p < 0.001). When we applied the models to the samples from the other two trials, we found a general decrease in their predictive ability. Compared with other grain traits (carbon isotope discrimination, grain weight, ash and nitrogen content), NIRS showed a similar or even stronger relationship with GY, at least when validated in the same trial. We concluded that NIRS could be used to rank GY during the early generations of a breeding program, when performing yield trials is less feasible. However, further research is required to identify the environments in which this technique is more reliable, as well as to evaluate the heritability of NIRS-predicted GY. 相似文献
8.
K. Kubo I. Elouafi N. Watanabe M. M. Nachit M. N. Inagaki K. Iwama Y. Jitsuyama 《Plant Breeding》2007,126(4):375-378
Increasing root penetration (RP) ability into hard soil is important to improve drought resistance in durum wheat. Traits related to RP ability were evaluated in 110 recombinant inbred lines (RILs) derived from a cross between 'Jennah Khetifa' and 'Cham1' using paraffin-Vaseline (PV) discs. QTL analyses were made for the number of roots penetrating the PV disc (PVRN), total number of seminal and crown roots (TRN), RP index (PVRN/TRN) and root dry weight (DW). 'Jennah Khetifa' had higher PVRN, RP index and root DW values than 'Cham1', and the RILs showed significant differences for these traits. Two closely-linked markers, Xgwm617a and Xgwm427b , on the long arm of chromosome 6A were associated with PVRN and RP index. For root DW, a QTL was linked to marker Xgwm11 on chromosome 1B. Alleles of 'Jennah Khetifa' were associated with increased PVRN, RP index and root DW. No QTL was detected for TRN in this mapping population. The absence of co-located QTLs suggested that RP ability was controlled separately from TRN and root DW. Although the population size and number of replications were small, this study helps in understanding the complexity of root growth and the potential of marker-assisted selection for selecting genotypes with high RP ability in durum wheat populations. 相似文献
9.
M. El Bouhssini M. M. Nachit J. Valkoun O. Abdalla F. Rihawi 《Genetic Resources and Crop Evolution》2008,55(8):1215-1219
The Hessian fly, Mayetiola destructor (Say), is a major pest of wheat in North Africa, southern Europe, North America, and northern Kazakhstan. It is believed
this pest (like wheat) originated in West Asia. The Syrian Hessian fly biotype has been found to be the most virulent worldwide,
and has been used at the International Center for Agricultural Research in the Dry Areas (ICARDA) for screening wheat and
its wild relatives to identify new sources of resistance. The screening was conducted in an insect rearing room set at 20°C
and 70% RH using a Hessian fly population collected from Lattakia region, Syria. The experimental design was a randomized
complete block with four replications. ‘Nasma’ (bread wheat) and ‘Cando’ (durum wheat) were used as susceptible and resistant
checks, respectively. A total of 623 lines/accessions of wheat and its wild relatives (Aegilops and Triticum) were evaluated. Twenty-nine Aegilops accessions and four synthetic derived bread wheat lines were found resistant. The presence of dead first instars confirmed
the resistance reaction and also showed that antibiosis is the major mechanism of resistance in these materials. These sources
of resistance are used in ICARDA’s wheat breeding programs for the development of Hessian fly-resistant germplasm/varieties. 相似文献
10.
Identification in Aegilops species of resistant sources to Hessian fly (Diptera: Cecidomyiidae) in Morocco 总被引:1,自引:0,他引:1
M. El Bouhssini O. Benlhabib M.M. Nachit A. Houari A. Bentika N. Nsarellah S. Lhaloui 《Genetic Resources and Crop Evolution》1998,45(4):343-345
Hessian fly, Mayetiola destructor (Say), is the major insect pest of wheat in Morocco. Host plant resistance has been the most effective and practical method of controlling this pest. When 347 accessions of Aegilops species were screened in the greenhouse for resistance to Hessian fly, several accessions of Ae. geniculata Roth, Ae. triuncialis L., Ae. neglecta Req.ex Bertol., Ae. ventricosa Tausch, Ae. cylindrica Host and Ae. markgrafii (Greuter) Hammer showed resistance reaction. All expressed antibiosis as the mechanism of resistance against first instar Hessian fly larvae. These Aegilops sources of resistance could be exploited for transferring Hessian fly resistance to wheat. 相似文献