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排序方式: 共有251条查询结果,搜索用时 15 毫秒
1.
Gripshover EM Givens MD Ridpath JF Brock KV Whitley EM Sartin EA 《Veterinary microbiology》2007,125(1-2):11-21
Bovine viral diarrhea virus (BVDV) affects cattle populations causing clinical signs that range from subclinical immunosuppression to severe reproductive and respiratory problems. Detection and removal of persistently infected (PI) calves is the single most important factor for control and eradication of BVDV. Current testing strategies to detect PI calves rely heavily on immunohistochemistry (IHC) and a commercially available antigen capture ELISA (ACE) assay. These viral assays depend on 1 or 2 monoclonal antibodies which target the E(rns) glycoprotein of BVDV. The sensitivity and specificity of these two tests have been reported previously. The purpose of this research was to characterize a strain of BVDV (AU501) that was undetectable using IHC and ACE based on a single monoclonal antibody, but was consistently detected in samples from a Holstein steer using virus isolation and PCR testing. Sequencing of this AU501 viral isolate revealed a unique mutation in the portion of the genome coding for the E(rns) glycoprotein. This unique field strain of BVDV demonstrates the risk of relying on a single monoclonal antibody for detection of BVDV. Multiple testing strategies, including polyclonal or pooled monoclonal antibodies that detect more than one viral glycoprotein may be necessary to detect all PI calves and facilitate eradication of BVDV. 相似文献
2.
Ryan B. Mounce Susan A. O’Shaughnessy Brock C. Blaser Paul D. Colaizzi Steven R. Evett 《Irrigation Science》2016,34(3):231-244
In the High Plains, corn (Zea mays L.) is an important commodity for livestock feed. However, limited water resources and drought conditions continue to hinder corn production. Drought-tolerant (DT) corn hybrids could help maintain high yields under water-limited conditions, though consistent response of such hybrids is unverified. In this two-year study, the effects of three irrigation treatments were investigated for a DT and conventional maize hybrid, Pioneer AQUAMax P0876HR and Pioneer 33Y75, respectively. In 2013, the drier of the 2 years, irrigation amounts and crop water use (ETc) were greater for the conventional hybrid, but grain water use efficiency (WUE) and harvest index were significantly greater for the DT hybrid. In 2014, grain yields and WUE were not significantly different between hybrids. However, irrigation amounts, ETc and biomass yields were greater for the conventional hybrid. Results from both years indicate that the DT hybrid required less water to maximize grain yield as compared to the conventional hybrid. Producing relatively high yields with reduced amounts of water may provide a means for producers to continue corn production in a semiarid environment with declining water supplies. 相似文献
3.
In trace metal (TM)-contaminated agricultural soils, management of TM availability is important for safe crop production. In addition, maintenance or improvement of soil quality is vital for sustainable crop cultivation. Decreased TM phytoavailability and increased soil quality can be achieved by the application of various immobilizing agents to soil, which can supply both macronutrients and organic matter. This study investigated the long-term influences of four common immobilizing agents on so... 相似文献
4.
Relation between soil organic matter and yield levels of nonlegume crops in organic and conventional farming systems 总被引:2,自引:0,他引:2
Christopher Brock Andreas Fließbach Hans‐Rudolf Oberholzer Franz Schulz Klaus Wiesinger Frank Reinicke Wernfried Koch Bernhard Pallutt Bärbel Dittman Jörg Zimmer Kurt‐Jürgen Hülsbergen Günter Leithold 《植物养料与土壤学杂志》2011,174(4):568-575
The aim of this study was to evaluate the interaction between yield levels of nonleguminous crops and soil organic matter (SOM) under the specific conditions of organic and conventional farming, respectively, and to identify implications for SOM management in arable farming considering the farming system (organic vs. conventional). For that purpose, correlations between yield levels of nonlegume crops and actual SOM level (Corg, Nt, Chwe, Nhwe) as well as SOM‐level development were examined including primary data from selected treatments of seven long‐term field experiments in Germany and Switzerland. Yield levels of nonlegume crops were positively correlated with SOM levels, but the correlation was significant only under conditions of organic farming, and not with conventional farming treatments. While absolute SOM levels had a positive impact on yield levels of nonlegumes, the yield levels of nonlegumes and SOM‐level development over time correlated negatively. Due to an increased demand of N from SOM mineralization, higher yield levels of nonlegumes obviously indicate an increased demand for OM supply to maintain SOM levels. Since this observation is highly significant for farming without mineral‐N fertilization but not for farming with such fertilization, we conclude that the demand of SOM‐level maintenance or enhancement and thus adequate SOM management is highly relevant for crop production in organic farming both from an agronomical and ecological point of view. Under conventional management, the agronomic relevance of SOM with regard to nutrient supply is much lower than under organic management. However, it has to be considered that we excluded other possible benefits of SOM in our survey that may be highly relevant for conventional farming as well. 相似文献
5.
Fatemeh Abbasi Mina Taghizadeh Babak ValizadehKaji 《International Journal of Fruit Science》2019,19(2):221-230
Prunus scoparia is a wild deciduous shrub, usually living on dry calcareous soils of the rocky mountains and has been used as a grafting rootstock for domesticated almonds to provide drought resistance. In the current study, micropropagation ability of P. scoparia was investigated using cytokinin and auxin. Uniform nodal shoot pieces (3–5 cm in length) of seedlings were used as explants. The explants were disinfected with 10% sodium hypochlorite solution. For adventitious shoot induction and proliferation, Murashige and Skoog (MS) media containing 7.00 g/l agar and 30.00 g/l sucrose containing five concentrations of benzyl adenine (BA) (0.00, 0.50, 1.00, 2.00, and 4.00 mg/1) and also containing six concentrations of Thidiazuron (TDZ) (0.00, 0.50, 1.00, 2.00, 5.00, and 7.00 mg/1) were compared. For rooting, in vitro shoots (2–3 cm) were transferred into ½ MS medium supplemented with 30 g/l sucrose, 7.50 g/l agar, and different concentrations of IBA (0.00, 0.25, 0.50, and 1.00 mg/l) and NAA (0.00, 0.25, 0.50, and 1.00 mg/l). Based on the results obtained for shoot proliferation, only 2.00 and 4.00 mg/l BA and 2.00 mg/l TDZ concentrations generated shoots, while other treatments did not show shoot proliferation. Among the three treatments that generated shoots, the best results for shoot number, leaf number, and leaf color quality were observed in media containing 2.00 mg/l TDZ. Based on the results obtained for rooting, the effect of IBA concentrations on the rooting percentage, root number, and root length was significant. Among IBA concentrations, only 0.50 mg/l IBA induced rooting, while there was no rooting in the media containing other IBA concentrations. None of the NAA concentrations showed rooting. In conclusion, MS culture medium supplemented with 2.00 mg/l TDZ and ½ MS culture medium supplemented with 0.50 mg/l IBA are suggested for in vitro shoot proliferation and rooting of P. scoparia, respectively. The results presented herein could be used for in vitro selection and micropropagation of P. scoparia. 相似文献
6.
7.
Brock KA 《Australian veterinary journal》2001,79(1):24-25
Although it is unwise to recommend any preanaesthetic drug or regimen to be used routinely without consideration for the individual patient, the procedure being undertaken or the other drugs that will be used during the anaesthetic, of all anaesthetic-related drugs that might be under review, atropine is probably one of the least 'toxic' and least likely to cause life-threatening complications when used correctly. In most small veterinary practices there isn't the luxury of a dedicated anaesthetist available to monitor and manage each anaesthetised patient and the best form of cardiac monitor available may be an audible rate monitor. In this situation the advantages combined with the usual indications probably far outweigh the disadvantages of using atropine prophylactically. If a choice must be made, a sinus tachycardia under anaesthesia is probably preferable to a profound bradyarrhythmia. Absolute contraindications for using atropine are rare; the drug, when used alone, has low incidence of toxicity at clinically recommended doses in dogs and cats. Further, the new inhalation agents available to veterinarians (enflurane and isoflurane) do not sensitise the heart to the effect of catecholamines, so that the predominance of sympathetic tone produced when atropine is used is unlikely to precipitate dysarrthythmias during gaseous anaesthesia. Rhythm disturbances that occur under halothane anaesthesia when atropine is used, suggest there is some other cause, or agent, that is arrhythmogenic, such as an alpha 2 agonist, excessively deep level of anaesthesia, hypercapnia or hypoxaemia. Notwithstanding the above arguments, my preference, as a specialist anaesthetist, is that a drug is given only as required (not before) and that when, for example, any unusual heart rhythm occurs, all possible reasons for the occurrence (such as excessively deep anaesthesia or hypoventilation) are eliminated before other drugs like atropine or antiarrhythmics are administered. 相似文献
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10.
d'Offay JM Floyd JG Eberle R Saliki JT Brock KV D'Andrea GH McMillan KL 《Journal of the American Veterinary Medical Association》2003,222(10):1404-7, 1366-7
Beef cattle from a herd in north Alabama were examined because of an outbreak of nonfatal skin disease characterized by discrete circumscribed areas of inflammation that developed on the skin from the neck to the hips. Areas of inflammation, which tended to be superficial, underwent necrosis and scabbed over. The scabs eventually dropped off leaving discrete, round, whitish, hairless lesions that were 1.2 to 2.5 cm diameter. Because clinical signs were consistent with those expected with pseudo-lumpy skin disease (PLSD) caused by bovine herpesvirus type 2 (BHV-2), samples from 16 representative animals were submitted for BHV-2 testing. All 16 animals were seropositive for BHV-2, but the virus could not be isolated from skin biopsy specimens or buffy coat samples. Results of a polymerase chain reaction assay incorporating primers designed to amplify 2 DNA sequences from BHV-2 were positive for 3 of the 10 cattle, suggesting that skin lesions in these cattle were a result of PLSD. Our findings suggest that PLSD may be more common and widespread in the United States than suggested by the frequency with which BHV-2 has been isolated from cattle with PLSD-like skin lesions. 相似文献