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Mi Jeong Park Jienny Lee Jeong Su Byeon Da-Un Jeong Na-Yeon Gu In-Soo Cho Sang-Ho Cha 《Veterinary research communications》2018,42(3):171-181
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days. Quantitative real-time PCR was performed to analyze gene expression. As results, expression levels of pluripotent markers were increased depending on spheroid size and the production of PGE2 was increased in spheroid formation compared to that in monolayer. Ki-67 showed a remarkable increase in the spheroid formed with 2.0?×?105 cells/drop as compared to that in the monolayer. Expression levels of angiogenesis-inducing factors such as VEGF, IL-6, IL-8, and IL-18 were significantly increased in spheroid formation compared to those in the monolayer. Expression levels of bone morphogenesis-inducing factors such as Cox-2 and TGF-β1 were also significantly increased in spheroid formation compared to those in the monolayer. Expression levels of osteocyte-specific markers such as RUNX2, osteocalcin, and differentiation potential were also significantly increased in spheroid formation compared to those in the monolayer. Therefore, spheroid formation of eAD-MSCs through the hanging drop method can increases the expression of angiogenesis-inducing and bone morphogenesis-inducing factors under optimal culture conditions. 相似文献
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Kim Do-Gyun Lee Seung-Hyun Cho Byoung-Kwan Byeon Dae-hyeon Lee Jayoung Lee Wang-Hee 《Journal of Crop Science and Biotechnology》2020,23(1):1-7
Journal of Crop Science and Biotechnology - Problems caused by unapproved living modified organism (LMO) seeds in seed imports have emphasized determination of sample size as the most fundamental... 相似文献
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Mice were intranasally inoculated at various times to optimize the vaccination strategy with a new live candidate vaccine expressing the antigens CP39, FimA, PtfA, and ToxA of Pasteurella multocida and F1P2 of Bordetella bronchiseptica in an attenuated live Salmonella system to protect against progressive atrophic rhinitis (PAR). Sixty BALB/c mice were divided equally into 4 groups. The group A mice were vaccinated only at 12 wk of age, the group B mice received a primary vaccination at 9 wk of age and a booster at 12 wk of age, the group C mice received a primary vaccination at 6 wk of age and boosters at 9 and 12 wk of age, and the group D mice were inoculated intranasally with sterile phosphate-buffered saline as a control. The humoral and mucosal immune responses of groups A, B, and C increased significantly compared with those of the control group. Expression of the cytokines interleukin-4 and interferon-γ in splenocytes also increased significantly. In addition, the group B mice exhibited significantly fewer gross lesions in lung tissue compared with the other vaccinated groups after challenge with a virulent P. multocida strain. These results indicate that a strategy of double intranasal vaccination can optimize protection against PAR. 相似文献
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Hyeon-Seop Byeon Mi Jung Ji Shin-Seok Kang Sang Woo Kim Seung-Cheol Kim Song-Yong Park Geehyuk Kim Jiro Kim Jang-Eun Cho Bok Kyung Ku Jae-Myung Kim Bo-Young Jeon 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):31-35
Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. In the present study, performance of the SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and M. bovis field isolates collected from animals. The SD MPT64 Rapid test produced positive results for 95.5% (63/66) of the M. bovis isolates from cattle and 97.9% (46/47) of the isolates from deer. Additionally, the test had a sensitivity of 96.5% (95% CI, 91.2-99.0), specificity of 100% (95% CI, 96.7-100.0), positive predictive value of 100% (95% CI, 96.7-100.0), and negative predictive value of 92.9% (95% CI, 82.7-98.0) for M. bovis isolates. In conclusion, the SD MPT64 Rapid test is simple to use and may be useful for quickly confirming the presence of M. bovis in animals. 相似文献
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Kim Do-Gyun Lee Seung-Hyun Cho Byoung-Kwan Byeon Dae-hyeon Lee Jayoung Lee Wang-Hee 《Journal of Crop Science and Biotechnology》2019,22(4):299-307
Journal of Crop Science and Biotechnology - The seeds of unapproved living modified organism (LMO) have caused environmental problems; therefore, there has been emphasis on intensive quarantine... 相似文献
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A field study evaluated the effects of NPK (21:17:17) and compost on vegetative growth, proteins, and carbohydrates of Moringa oleifera. The experimental design was randomized complete block design (RCBD) with six treatments replicated three times. Compost and NPK fertilizers were applied together which include control (without fertilizer) 5 t ha?1 (Compost), 120 kg ha?1 (NPK), 50 + 50% ha?1 (NPK + Compost), 10 + 50% ha?1 (NPK + Compost), and 50 + 10% ha?1 (NPK + Compost) per plot. For NPK fertilizer, the rate was 10 and 50% of 120 kg ha?1 and for compost it was 10 and 50% of 5 t ha?1. The measured growth parameters were plant height (cm), stem girth (mm), number of leaves, and number of branches per plant. Results showed that 120 kg ha?1 (NPK) treatment produced plants of more height, stem girth, more number of leaves, maximum number of branches as compared to other treatments at week 8 with higher protein. Carbohydrate content was high in 50 + 50% (NPK + Compost) compared to others. 相似文献
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Ji-Hey Lim Chang-Su Jung Ye-Eun Byeon Wan Hee Kim Jung-Hee Yoon Kyung-Sun Kang Oh-kyeong Kweon 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):89-94
A model that provides reproducible, submaximal yet sufficient spinal cord injury is needed to allow experiments leading to development of therapeutic techniques and prediction of clinical outcome to be conducted. This study describes an experimental model for spinal cord injury that uses three different volumes of balloon inflation and durations of compression to create a controlled gradation outcome in adult dogs. Twenty-seven mongrel dogs were used for this study. A 3-french embolectomy catheter was inserted into the epidural space through a left hemilaminectomy hole at the L4 vertebral arch. Balloons were then inflated with 50, 100, or 150 µl of a contrast agent at the L1 level for 6, 12, or 24 h and spinal canal occlusion (SCO) measured using computed tomography. Olby score was used to evaluate the extent of spinal cord injury and a histopathologic examination was conducted 1 week after surgery. The SCO of the 50, 100, and 150 µl inflations was 22-46%, 51-70%, and 75-89%, respectively (p < 0.05). Olby scores were diminished significantly by a combination of the level of SCO and duration of inflation in all groups. Olby scores in the groups of 150 µl-12 h, 150 µl-24 h, and 100 µl-24 h were 0.5, 0, and 1.7, respectively. Based on these results, a SCO > 50% for 24 h, and > 75% for 12 h induces paraplegia up to a week after spinal cord injury. 相似文献
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Inhong Song Ik-Jae Kim Dae Ho Han Myeong-Seop Byeon Jae-Kwan Lee Moon-Seong Kang 《Paddy and Water Environment》2012,10(1):49-58
A majority of streams in Korea have been channelized and their adjacent flood plains have been converted for anthropogenic
land use, especially in urbanized areas. Fortunately, recent elevated public recognition to the stream ecosystem has led to
governmental efforts to conserve riparian areas. In this study, a simple method to prioritize locations for riparian establishment
was developed at a watershed scale based on spatiotemporal change of riparian forest area. The developed method was applied
for the Ansung and Sapkyo watersheds, which were under consideration for the stream riparian area establishment by the Korean
Ministry of Environment. Two riparian forest indices, Riparian Forest Index (RFI) and Riparian Forest Change Index (RFCI)
were developed to represent spatial and temporal change of watershed riparian forest, respectively. LANSAT satellite images
with a 30 m × 30 m resolution were used to estimate the two riparian forest indices. A precautionary approach, which intends
to preserve the existing riparian forests as much as possible, was applied by ranking sub-watersheds based on the two riparian
forest indices to prioritize locations for the riparian establishment at a sub-watershed level. The results showed that overall
urban land cover in riparian areas increased while forests and cropland decreased over the past 25 years. More importantly,
riparian forest removal occurred more rapidly in the riparian area, which is one of the most important niches for riparian
ecosystems, as compared to the entire watershed. Most riparian forests appeared to be located upstream of the watersheds,
and thus it is important to develop management measures to preserve existing riparian forests from human activities. The developed
approach could be a useful tool that can assist policy makers to prioritize locations for the riparian area establishment.
However, this method has limitations of only considering riparian forest area and therefore, other aspects such as stream
morphology as well as ecology needs to be incorporated into riparian area determination process as they become available in
the future. In addition, considering that substantial portions of riparian areas have already been disturbed, the restoration
aspect of the impaired riparian also needs to be investigated further. 相似文献