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Bryant RJ Rao DR Ogutu S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2003,58(3):213-223
Thirty-three peanut cultivars were examined for their alpha-1,6 and beta-1,4 galactosidase activities and oligosaccharide contents along with proximate compositions. The average moisture, protein, fat, ash, and carbohydrate contents were: 4.9%, 26.6%, 43.1%, 2.3% and 23.1%, respectively. The corresponding coefficients of variation were: 5.2%, 10.1%, 7.2%, 7.8% and 15.7%, respectively. Raffinose and stachyose contents (%) ranged from 0.05 to 0.12 and 0.31 to 0.61, respectively. The specific activity (micromol product/min/mg protein) of crude preparation of alpha-galactosidase for the 33 cultivars ranged from 1.096 to 2.784 for the non-germinated seeds and from not being detected in some samples up to 2.432 for the germinated seeds; the mean values for non-germinated and germinated seeds were: 1.781 and 1.410, respectively. The specific activity of beta-galactosidase ranged from 0.101 to 1.727 in the non-germinated seeds and from not being detected in some samples up to 0.898 in the germinated seeds. Germination decreased the activity of both galactosidases significantly (p < or = 0.05). 相似文献
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Chukwumah YC Walker LT Verghese M Bokanga M Ogutu S Alphonse K 《Journal of agricultural and food chemistry》2007,55(2):285-290
Peanuts have been reported to contain bioactive phytochemicals, particularly isoflavones (genistein, daidzein, and biochanin A) and trans-resveratrol. Currently, limited data are available regarding the levels of these bioactive compounds in peanuts with variations in reported levels. The purpose of this study was to compare four methods of extraction [stirring, sonication, Soxtec, and microwave-assisted sonication (MAS)] for runner peanuts. Quantification of the selected compounds was conducted by reverse-phase high-performance liquid chromatography (RP-HPLC). The results showed that the MAS and Soxtec methods extracted significantly higher amounts of the phytochemicals. Also, the defatted peanuts gave significantly higher amounts of the phytochemicals compared to the nondefatted peanuts. The high levels of the isoflavones may be attributed to heat-induced conversion of conjugate glycosides to aglycons. The MAS and Soxtec methods may be used for total isoflavone content quantitation, while sonication or stirring may be the method of choice for quantitation of isoflavone composition (aglycons and glycoside conjugates) in peanuts. 相似文献
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Hans-Peter Piepho Joseph O. Ogutu 《Journal of Agricultural, Biological & Environmental Statistics》2002,7(3):350-360
This article is concerned with a test for time trend in ecological surveys conducted at multiple sites across several years. The test is useful for studying long-term trends in wildlife populations. We propose a modification of a mixed model—recently suggested by Van Leeuwen, Murray, and Urquhart—which leads to invariance to translation in the origin of the time covariate. The modified model is easily fitted using standard software. Using simulation, the performance of the procedure for un balanced data is shown to be satisfactory. Data on the population density of Thomson’s gazelles (Gazella thomsoni Günther) are used as an example. 相似文献
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Sabbione Ana Clara Ogutu Fredrick Onyango Scilingo Adriana Zhang Miao Añón María Cristina Mu Tai-Hua 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(1):107-114
Antiproliferative effect of Amaranthus mantegazzianus proteins and peptides released after simulated gastrointestinal digestion (DH% 37.8?±?3.8) was investigated on human colon cancer cell line HT-29. Inhibition of proliferation of HT-29 cells was exhibited after a 24 h treatment with different concentrations of amaranth protein isolate (API) and the peptides released after digestion (DGS), presenting IC50 values of 1.35?±?0.12 and 0.30?±?0.07 mg soluble protein/mL, respectively. Lactate dehydrogenase assay indicated that both samples caused the loss of membrane integrity and cell lysis over HT-29 cells, and DAPI fluorescence microscopies evidenced typical apoptotic features. Moreover, Annexin V-FITC flow cytometry showed a significant increase of early apoptotic and late apoptotic/necrotic HT-29 cells compared to untreated ones, and caspase-3 assay confirmed the apoptosis induction with a 43.0?±?10.3 and 65.8?±?12.7% increase of caspase-3 activity produced by a 2 mg/mL treatment of API and DGS, respectively. In conclusion, amaranth peptides successfully released after simulated gastrointestinal digestion would exert a potential antiproliferative activity over HT-29 tumor cells. This effect was linked to the induction of cell necrosis and apoptosis, supporting the idea of using amaranth proteins as a potential food alternative ingredient for functional foods.
相似文献5.
Z. A. Ogutu 《Land Degradation \u0026amp; Development》2002,13(3):251-256
This paper presents the impact of ecotourism on livelihood and natural resource management in the periphery of Amboseli Biosphere Reserve in Kenya. Ecotourism initiatives that have been introduced by Porini Ecotourism, a private investor, are benefiting Eselenkei Group Ranch in terms of income, improved infrastructure, employment opportunities and exposure. Over US$5000 is received annually as land rent, gate fee and bed charges. Twenty‐six Maasai men are employed for the upkeep of project facilities. The community's capacity to facilitate resource‐related conflicts has improved following support from development institutions. An expanding livelihood base is reducing local vulnerability to disaster and people–wildlife conflicts. The numbers of resident wildlife species in the conservation area have increased due to regeneration of woody species and reduced frequency of livestock. Despite the achievements, ecotourism is threatened by cultivation. The latter is jeopardizing conservation efforts, as the area frequented by wildlife is being lost and people–wildlife conflicts intensified. Another dilemma is that the Eselenkei community is not effectively participating in ecotourism a situation that is associated with inadequate management and negotiation skills in the group ranch committee. The latter requires leadership and microenterprise management skills if earnings from ecotourism are to be effectively invested in alternative sources of livelihood, to reduce current and potential conflicts. There is also need to build the community's capacity for the promotion of activities that compliment ecotourism. Frequent breakdown of community boreholes lead to dependence on the conservation area for water during prolonged drought, intensifying conflicts between livestock and wildlife. 1 Referring to low‐impact nature‐based tourism packages that benefit local communities and the national government while helping to conserve the resource base (Kenya Wildlife Service (KWS), unpublished report, 2001). Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
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Torben Schulz‐Streeck Joseph O. Ogutu Andrés Gordillo Zivan Karaman Carsten Knaak Hans‐Peter Piepho 《Plant Breeding》2013,132(6):532-538
Genomic selection has been routinely implemented in plant breeding in two stages. The first stage usually omits the marker information and estimates adjusted means of genotypes across environments. The second stage uses the adjusted means to predict genomic breeding values. However, if the effects of markers vary substantially between different environments, it may be important to account for this variation for varieties adapted to different environments. Using two maize data sets, we investigated whether modelling the marker‐by‐environment interaction can improve the predictive ability of genomic selection relative to modelling genotype‐by‐environment interaction alone. Modelling the marker‐by‐environment interaction did not substantially increase the predictive ability relative to modelling only the genotype‐by‐environment interaction for the two tested data sets. Thus, genomic selection, carried out in a stagewise fashion, such that the marker information is omitted until the last stage of the process, may suffice for most practical purposes. Moreover, predictive ability did not reduce substantially even when the number of markers with consistent effects across environments used for genomic prediction was reduced to about 50. 相似文献
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R. Mangeni‐Sande A. Taabu‐Munyaho R. Ogutu‐Ohwayo W. Nkalubo V. Natugonza H. Nakiyende C. S. Nyamweya V. B. Muwanika 《Fisheries Management and Ecology》2019,26(5):406-412
The small pelagic cyprinid, Rastrineobola argentea (Pellegrin), commonly known as dagaa, accounted for 60% of the total fish biomass and 40% of the commercial catches in Lake Victoria in 2015. However, some aspects of the biology of species (from which management interventions are based) have changed since 1970s; and yet harvest regulations have remained the same. In this study, spatial and temporal variations in life history traits of dagaa in the northern portion of Lake Victoria were examined in relation to fishing intensity to offer guidance on possible adjustments in managing the fishery. The mean standard length halved, whilst the length at 50% maturity (Lm50) reduced by 27%, between the 1970s and 2015; however, the decline in Lm50 was more pronounced in males than females. Data collected between 2014 and 2015 showed that immature individuals are largely harvested from inshore and mid‐island areas, whilst most of the fishes caught in open water areas are largely mature irrespective of the size of the gear used. The causes of the changes in these biological aspects, and the need for policy adjustment, are discussed in the context of changes in fishing pressure. 相似文献
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Inland fisheries play important roles in food and economic security in the riparian countries surrounding the Great Lakes of Africa. However, the lakes are being systematically degraded by anthropogenic pressures, in combination with the huge population growth prevalent in the region. This paper summarises the outcomes of an international conference to develop a ‘Strategy for Conservation and Sustainable Development of the African Great Lakes Region in a Changing Climate’, held in Uganda in 2017, with particular attention on the potential for fisheries and aquaculture. The paper highlights options for addressing the problems facing the aquatic resources and specifically the importance of effective management of the fisheries and ecosystems of the Great Lakes to achieving the UN Sustainable Development Goals to ensure food and nutritional security and sustainable livelihoods. There is need to improve fisheries assessment and determine the value of the fisheries (and aquaculture). Fisheries legislation, regulation and enforcement need revision and co‐management mechanisms require rethinking. There is considerable potential for aquaculture, and especially cage culture, to increase fish production, but there is an urgent need to address technical, social, environmental and input requirements. It is also imperative that best practices guidelines are developed that will support cage culture production practices. 相似文献
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