Production of patchouli mild mosaic virus resistant patchouli plants by genetic engineering of coat protein precursor gene   总被引：2，自引：0，他引：2  

Kadotani N  Ikegami M 《Pest management science》2002,58(11):1137-1142

Patchouli (Pogostemon cablin (Blanco) Benth), an aromatic crop which yields an essential oil, is widely cultivated in South-east Asia. Patchouli mild mosaic virus (PaMMV) infects patchouli plants and causes decrease in leaf biomass and essential oil yield. Transgenic patchouli plants with PaMMV coat protein precursor (CP-P) gene have been produced by Agrobacterium-mediated transformation. PaMMV CP-P gene integration into the patchouli genome was confirmed by the PCR method and by Southern blot analysis. The transformants were estimated to contain one to three copy genes using Southern blot analysis. The transformant with three copy genes was tested for the resistance to PaMMV by artificially inoculating plants grown in an environmentally controlled cabinet, and this transformant was found to be highly resistant to PaMMV. The transgenic patchouli plant with PaMMV CP-P gene should provide valuable material for protecting against PaMMV.  相似文献   

Growth, Net Photosynthetic Rate, Nutrient Status and Secondary Xylem Anatomical Characteristics of Fagus Crenata Seedlings Grown in Brown Forest Soil Acidified with H2SO4 Solution     

Takeshi Izuta  Taeko Yamaoka  Tatsuro Nakaji  Tetsushi Yonekura  Masaaki Yokoyama  Hideyuki Matsumura  Sachie Ishida  Kenichi Yazaki  Ryo Funada  Takayoshi Koike 《Water, air, and soil pollution》2001,130(1-4):1007-1012

Dry matter production, net photosynthetic rate, leaf nutrient status and trunk anatomical characteristics of Fagus crenata seedlings grown in brown forest soil acidified by adding H₂SO₄ solution were investigated. The soil acidification leaded to decreased (Ca+Mg+K)/Al molar ratio in the soil solution. Dry mass per plant of the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1 was significantly reduced compared with the control value at 0 mg·L^?1. When net photosynthetic rate was reduced in the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1, the carboxylation efficiency and maximum net photosynthetic rate at saturated CO₂-concentration were lower than the control values. The addition of H⁺ to the soil at 120 mg·L^?1iinduced a reduction in the concentration of Ca in the leaf. By contrast, the concentration of Al in the leaf was increased with increasing the amount of H⁺ added to the soil. The annual ring formed in the seedlings grown in the soil treated with H⁺ at 120 mg·L^?1 was significantly narrower than that at 0 (control), 10, 30, 60 or 90 mg·L^?1. Based on the results obtained in the present study, we conclude that Fagus crenata is relatively sensitive to a reduction in the (Ca+Mg+K)/Al molar ratio of soil solution compared with Picea abies.  相似文献   

Net requirements of calcium, magnesium, sodium, phosphorus, and potassium for growth of Nellore × Red Angus bulls, steers, and heifers   总被引：1，自引：0，他引：1  

Mario Luiz Chizzotti  Sebastio de Campos Valadares Filho  Luis Orlindo Tedeschi  Pedro Veiga Rodrigues Paulino  Mrio Fonseca Paulino  Rilene Ferreira Diniz Valadares  Paloma Amaral  Pedro Del Bianco Benedeti  Tainnah Ikegami Rodrigues  Mozart Alves Fonseca 《Livestock Science》2009,124(1-3):242-247

A comparative slaughter trial was conducted to assess the net requirements for gain of Ca, P, Na, K and Mg of bulls, steers and heifers of Nellore and Red Angus crossbreds. Twenty seven F1 Nellore and Red Angus crossbred calves (9 steers, 9 bulls, and 9 heifers), averaging 274 kg BW, were used. At the beginning of the trial, three animals from each gender were slaughtered to determine the initial body composition. The remaining 18 animals (3 animals of each gender) were randomly assigned to two treatments: fed 0.75 or 1.5% of BW of concentrate. The diets were based on corn silage and were isonitrogenous (2% N, DM). After three growing periods of 28 d, all animals were slaughtered. The cleaned gastrointestinal tract, organs, carcass, head, hide, tail, feet, and tissues were weighed to determine the empty BW (EBW). These parts were ground separately and subsampled for chemical analyses. The log of the contents of each mineral in the empty body was regressed on the log of the EBW to estimate the net requirement for each mineral per kg of empty body gain (EBG). There were no differences (P > 0.05) in the net requirements for growth of all macrominerals among genders. The equations of the pooled data of the net requirements for growth (g/kg EBG) were: 332.6 × EBW ^− 0.6367 for Ca, 112.1 × EBW ^− 0.5615 for P, 10.85 × EBW ^− 0.3992 for Na, 4.01 × EBW ^− 0.153 for K, and 3.589 × EBW ^− 0.462 for Mg. Our findings indicated that retained Ca and retained P were poorly related to the retained protein.  相似文献   

RNA-silencing suppressors of geminiviruses   总被引：2，自引：0，他引：2  

Pradeep Sharma  Masato Ikegami 《Journal of General Plant Pathology》2008,74(3):189-202

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First report of a strain of Tobacco leaf curl Japan virus associated with a satellite DNA in honeysuckle in Japan     

T. Ogawa  P. Sharma  M. Ikegami 《Plant pathology》2008,57(2):391-391

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Effect of boiled barley-rice-feeding in hypercholesterolemic and normolipemic subjects     

Sachie Ikegami  Masako Tomita  Setsuko Honda  Momoko Yamaguchi  Reiko Mizukawa  Yoko Suzuki  Keiko Ishii  Saeko Ohsawa  Nanako Kiyooka  Mitsuru Higuchi  Shuhei Kobayashi 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):317-328

Barley contains approximately 10% dietary fiber and is easily cooked with rice, the dominant cereal in Japan, to increase the intake of dietary fiber. This research involved three experiments to examine the influence of barley on blood lipids in human subjects. All subjects received a boiled barley-rice (50/50 w/w mix) supplement two times per day in place of rice for 2 or 4 weeks. In the normolipemic subjects, serum lipids were unaffected by the ingestion of barley for 4 weeks. In twenty hypercholesterolemic men aged 41 ± 5 years, the ingestion of barley was associated with a significant fall in serum total cholesterol, LDL-cholesterol, phospholipids and LDL and VLDL-lipoproteins. In seven mildly hypercholesterolemic women aged 56±7 years, a significant improvement of serum lipid profiles was observed. The present study suggests the possibility that the ingestion of barley-rice could lower serum lipids in hypercholesterolemic subjects.  相似文献   

The Natural Occurrence of Two Distinct Begomoviruses Associated with DNAbeta and a Recombinant DNA in a Tomato Plant from Indonesia     

Kon T  Hidayat SH  Hase S  Takahashi H  Ikegami M 《Phytopathology》2006,96(5):517-525

ABSTRACT Two begomoviruses (Java virus-1 and Java virus-2), two satellite DNAs (DNAbeta01 and DNAbeta02), and a recombinant DNA (recDNA) were cloned from a single tomato plant from Indonesia with leaf curl symptoms, and the role of these satellite DNAs in the etiology of begomovirus disease was investigated. The genome organizations of the two viruses were similar to those of other Old World monopartite begomoviruses. Comparison of the sequences with other begomoviruses revealed that Java virus-1 was a newly described virus for which the name Tomato leaf curl Java virus (ToLCJAV) is proposed. Java virus-2 was a strain of Ageratum yellow vein virus (AYVV) (AYVV-[Java]). ToLCJAV or AYVV-[Java] alone did not induce leaf curl symptoms in tomato plants. However, in the presence of DNAbeta02, both ToLCJAV and AYVV-[Java] induced leaf curl symptoms in tomato plants. In the presence of DNAbeta01, these viruses induced mild leaf curl symptoms in tomato plants. The recDNA had a chimeric sequence, which arose from recombination among ToLCJAV, AYVV-[Java], DNAbeta01, and DNAbeta02; it was replicated only in the presence of AYVV-[Java] in tomato plants.  相似文献   

Increase in telencephalic dopamine and cerebellar norepinephrine contents by hydrostatic pressure in goldfish: the possible involvement in hydrostatic pressure-related locomotion     

Taro Ikegami  Akihiro Takemura  Eunjung Choi  Atsushi Suda  Shozo Tomonaga  Muhammad Badruzzaman  Mitsuhiro Furuse 《Fish physiology and biochemistry》2015,41(5):1105-1115

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Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil,Chrysiptera cyanea     

Muhammad Badruzzaman  Satoshi Imamura  Yuki Takeuchi  Taro Ikegami  Akihiro Takemura 《Fish physiology and biochemistry》2015,41(1):61-71

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High-Fat diet enhances accumulation of hexachlorobenzene in rat dams and delays its transfer from rat dams to suckling pups through milk     

Nakashima Y  Ohsawa S  Ikegami S 《Journal of agricultural and food chemistry》1999,47(4):1587-1592

Effect of diets on the distribution and transfer of hexachlorobenzene (HCB) from dams to fetuses and suckling pups was investigated. In pregnant rats, the amount of HCB accumulated in fat tissues of the high-fat diet group was higher than that of the control diet group (P < 0.05). The amounts of HCB in fetuses of the high-fat and control diet groups were estimated to be about 0.28 and 0.12% of the dam's total intake during pregnancy, respectively. In both groups, a large proportion of HCB in dams disappeared during lactation period and was transferred to their pups through the milk. In the pups of the high-fat diet group, the amount of HCB in stomach contents was lower immediately after birth and decreased slowly compared with that in the control diet group during lactation. These results showed that a high-fat diet reduced the speed of the transfer of HCB from the dams to their suckling pups through milk.  相似文献