A physical map of the 1-gigabase bread wheat chromosome 3B   总被引：2，自引：0，他引：2  

Paux E  Sourdille P  Salse J  Saintenac C  Choulet F  Leroy P  Korol A  Michalak M  Kianian S  Spielmeyer W  Lagudah E  Somers D  Kilian A  Alaux M  Vautrin S  Bergès H  Eversole K  Appels R  Safar J  Simkova H  Dolezel J  Bernard M  Feuillet C 《Science (New York, N.Y.)》2008,322(5898):101-104

As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.  相似文献   

Investigating the effect of yarn count and twist factor on the packing density and wicking height of lyocell ring-spun yarns     

Mostafa Taheri  Morteza Vadood  Majid Safar Johari 《Fibers and Polymers》2013,14(9):1548-1555

The moisture transport expressed with wicking is one of the most important aspects in clothing science and strongly effects on the quality of clothes. Wicking is a spontaneous transport of liquid driven into a porous system by capillary forces. Furthermore, the packing density has a direct relation with the yarn structure. At the present work, the effects of yarn count and twist factor on the wicking height and packing density of lyocell ring-spun yarns was investigated. Achieving the objectives of this research, an image processing method was developed to determine the packing density of samples. Experimental results were also used to develop a regression model to predict the wicking height based on the packing density, yarn count, twist factor and rising time. The results demonstrated that the correlation coefficient between the predicted and measured wicking height was 0.98 indicating the capability of the presented model to predict the wicking height of lyocell ring-spun yarns.  相似文献   

Investigating the CYP2B6 rs3745274 and rs3211371 Polymorphisms in Methadone-Responder and Non-Responder Addicts in Iran     

Sara Sadat Aghabozorg Afjeh  Behzad Boshehri  Safar Hamednia  Asmaolhosna Asmaolhosna  Parisa Mashayekhi  Mir Davood Omrani 《Iranian Biomedical Journal》2021,25(3):220

Background:Methadone therapy is a major protocol in opioid addiction cases in many health care systems. Population-based studies have shown that in addicted people, the genetic profile affects their response to methadone therapy. Therefore, this study designed to examine the frequency of two SNPs of the CYP2B6 gene (rs3745274 and rs3211371) in addicted cases in two methadone-responders and methadone non-responders groups. Methods:A total of 199 opioid-addicted individuals and 117 unaffected control subjects were genotyped for rs3745274 and rs3211371 polymorphisms of the CYP2B6 gene using the tetra-primer ARMS-PCR. Results:Results of this study revealed the significant association of rs3745274 GG (p < 0.001; OR = 0.027; 95% CI = 0.14-0.49) and GT (p < 0.001; OR = 4.04; 95% CI = 2.26-7.21) genotypes with the risk of addiction in methadone-responders. Also, a significant association between rs3745274 GG (p < 0.001; OR = 0.28; 95% CI = 0.15-0.51) and GT (p < 0.001; OR = 5.1; 95% CI = 2.8-5.28) genotypes and addiction relapse was found in methadone non-responders. Conclusion:Based on our findings, we can conclude that rs3745274 variant of CYP2B6 gene could serve as a potential biomarker, to evaluate the prognosis of addicted people fate under treatment with methadone. Key Words: Addiction, Biomarker, Methadone, Single-nucleotide polymorphism  相似文献   

Analysis of genotypic variation for normalized difference vegetation index and its relationship with grain yield in winter wheat under terminal heat stress     

Shahnoza Hazratkulova  Ram C. Sharma  Safar Alikulov  Sarvar Islomov  Tulkin Yuldashev  Zafar Ziyaev  Zakir Khalikulov  Zokhid Ziyadullaev  Jozef Turok 《Plant Breeding》2012,131(6):716-721

Normalized difference vegetation index (NDVI), which is a measure of leaf greenness (chlorophyll content), is considered to be correlated with crop productivity. This study was conducted to examine genotypic variations for NDVI at different growth stages and its relationship to yield in winter wheat under terminal heat stress. Thirty winter wheat genotypes were evaluated at two locations in 2009–2010 and 2010–2011 in Uzbekistan. The NDVI was recorded at booting, heading, milk and dough stages. The wheat genotypes differed significantly for NDVI at each stage. Grain yield ranged from 3.9 to 6.1 t/ha. Wheat genotypes differed in per cent decline in NDVI from booting to dough stage. However, several high‐yielding genotypes maintained higher NDVI than low‐yielding genotypes when heat stress was evident. The findings suggest change in NDVI during heat stress could be a measure of tolerance. The positive correlation of NDVI with grain yield suggests that it could be used as an indirect selection criterion for identifying physiologically superior, high‐yielding wheat lines under terminal heat stress.  相似文献   

Trend analysis of reference evapotranspiration in the western half of Iran   总被引：6，自引：0，他引：6  

Hossein Tabari  Safar MarofiAli Aeini  Parisa Hosseinzadeh TalaeeKurosh Mohammadi 《Agricultural and Forest Meteorology》2011,151(2):128-136

Reference evapotranspiration (ET_o) is an important element of the hydrological cycle, and changes in ET_o are of great significance for agricultural water use planning, irrigation system design and management. In this study, annual, seasonal and monthly trends in the Penman-Monteith ET_o at 20 meteorological stations during 1966-2005 in the western half of Iran were examined using the Mann-Kendall test, the Sen's slope estimator and the linear regression. Annual analysis of the ET_o series indicated a positive trend in 70% of the stations according to the Mann-Kendall test and the Sen's slope estimator and in 75% of the stations according to the linear regression. The magnitude of significant positive trends in annual ET_o varied from (+)11.28 to (+)2.30 mm/year. On the seasonal scale, stronger increasing trends were identified in ET_o data in winter and summer compared with those in autumn and spring. Meanwhile, the highest numbers of stations with significant trends were found in the monthly ET_o series in February, while the lowest numbers of stations with significant trends were observed in November. Analysis of the impact of climatic variables on the significant increasing trend in ET_o showed that the increasing trend was mainly caused by a significant increase in air temperature during the study period.  相似文献   

Immunodiagnostics of Toxocara canis in suspected ocular and visceral manifestations     

E H Safar  M E Azab  H M Khalil  M A Bebars  H el Hady  H M Khattab 《Folia parasitologica》1990,37(3):249-254

Sera from patients with ocular and visceral manifestations of toxocariasis were tested by precipitin absorption (PAT), counter-immunoelectrophoresis (CIEP), indirect fluorescent antibody (IFAT) and ELISA. The ocular cases revealed a percentage of positivity of 36% by PAT, 0% by CIEP, 32% and 28% by IFAT with embryonated egg (EE) and frozen section antigens (FS) respectively and 40% by ELISA. The visceral cases revealed 44% positively by PAT, 24% by CIEP 52% and 48% by IFAT with EE and FS respectively and 52% by ELISA. Statistical evaluation was done to interpret the results.  相似文献   

Cloning,Expression and Characterization of Recombinant Exotoxin A-Flagellin Fusion Protein as a New Vaccine Candidate against Pseudomonas aeruginosa Infections     

Asghar Tanom  Safar Farajnia  Shahin Najar Peerayeh  Jafar Majidi 《Iranian Biomedical Journal》2013,17(1):1-7

Background: Infections due to Pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. At present, aggressive antibiotic therapy is the only choice for management of P. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alternative therapeutics including an effective vaccine. Several P. aeruginosa antigens have been tested for vaccine development, including lipopolysaccharide alone, polysaccharides alginate, extracellular proteins, exotoxin A (exo A) and killed whole cell. However, none of them are currently available clinically. Methods: In this research, recombinant exoA-flagellin (fliC) fusion protein as a cocktail antigen was expressed and purified and its antigenic characteristics were evaluated. Results: Expression of recombinant fusion protein by E. coli using pET22b vector resulted in production of exoA-fliC fusion protein in high concentration. Based on Western-blotting results, recombinant fusion protein showed a good antigenic interaction with sera from patients with various P. aeruginosa infections. Conclusion: These results suggested that recombinant exoA-fliC fusion protein can be produced in the laboratory, and tested as a candidate vaccine in P. aeruginosa infections. Key Words: Pseudomonas aeruginosa, Exotoxin A (exoA), Flagellin (fliC), Vaccines  相似文献   

A serologic study on Toxoplasma gondii infection in slaughtered sheep and goats in Qazvin Province,Iran     

Izadyar  Nasrin  Abd Nikfarjam  Bahareh  Esmaeili Rastaghi  Ahmad Reza  Alizadeh  Safar Ali  Heydarian  Peyman  Saraei  Mehrzad 《Tropical animal health and production》2019,51(5):1289-1293

Background

Toxoplasma gondii is a common protozoan parasite among all mammals, in particular small ruminants, worldwide. Traditional husbandry can be a major risk factor for infection of sheep and goats with this parasite.

Objectives

The present study aimed to determine the current status of the prevalence for T. gondii in livestock of Qazvin Province.

Methods

In this cross-sectional study, the sera of 455 sheep and 375 goats were examined to detect anti-Toxoplasma IgG antibodies by using in-house indirect ELISA.

Results

Overall, 33.62% (153/455) of sheep and 36.41% (130/375) of goats were positive for anti-Toxoplasma IgG antibodies with no statistically significant difference. The prevalence rate of T. gondii among the sheep of Qazvin County was significantly higher than in Abyek and Abhar counties (p < 0.001).

Conclusions

The results of the present study indicate that the prevalence of T. gondii in sheep and goats of the study area is high. Therefore, the meat of the animals reared in this area can be a potential source of human infections by this parasite.

  相似文献   

Modeling spun yarns migratory properties using artificial neural network     

Parham Soltani  Morteza Vadood  Majid Safar Johari 《Fibers and Polymers》2012,13(9):1190-1195

The mechanical and physical properties of spun yarns and fabrics depend not only on properties of constituent fibers, but also the yarn structure characterized by geometrical arrangement of fibers in the yarn body. Although there are many studies related to analyzing the migratory properties of spun yarns, there are no studies available about predicting yarn migration parameters. Therefore, the main aim of this research is to introduce a new approach to predict migratory properties of different kinds of spun yarns, namely siro, solo, compact and conventional ring-spun yarns. To achieve the objectives of the research, general physical and mechanical properties of spun yarns together with existing standards were thoroughly studied. Spun yarn migratory properties were predicted using intelligent technique of artificial neural network (ANN). Results signified that the ANN models can predict precisely the yarn migratory properties on the basis of a series of yarn physical and mechanical properties.  相似文献   

Estimation of daily pan evaporation using artificial neural network and multivariate non-linear regression   总被引：4，自引：1，他引：3  

Hossein Tabari  Safar Marofi  Ali-Akbar Sabziparvar 《Irrigation Science》2010,28(5):399-406

Measurement of evaporation (E) rate from various natural surfaces is known as the key element in any hydrological cycle and hydrometeorological studies. Due to the shortage of pan evaporation (E _P) data, the estimation of E _P for such studies seems necessary. The main aim of this paper was to estimate daily E _P using artificial neural network (ANN) and multivariate non-linear regression (MNLR) methods in semi-arid region of Iran. Five different ANN and MNLR models comprising various combinations of daily meteorological variables, that is, relative humidity (RH), air temperature (T), solar radiation (SR), wind speed (U) and precipitation (P) were developed to evaluate degree of effect of each of these variables on E _P. The comparison of models estimates showed that the ANN 5 model characterized by Delta-Bar-Delta learning algorithm and Sigmoid activation function which uses all input parameters (T, U, SR, RH, P) performed best in prediction of daily E _P. The sensitivity analysis revealed that the estimated E _P data are more sensitive to T and U, respectively. A comparison of the model performance between ANN and MNLR models indicated that ANN method presents the best estimates of daily E _P.  相似文献