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Fathia Aouidi Nathalie DupuyJacques Artaud Sevastianos RoussosMonji Msallem Isabelle Perraud GaimeMoktar Hamdi 《Industrial Crops and Products》2012,37(1):292-297
Oleuropein, the major active compound in olive leaf, is well known for its benefits for human health. Oleuropein is classically quantified by HPLC, which is time and chemical consuming, laborious and expensive. The aim of this work was to examine the potential of mid-infrared spectroscopy, as a rapid tool, to predict oleuropein content in olive leaf from five Tunisian cultivars (Chemlali, Chetoui, Meski, Sayali and Zarrazi) and one French cultivar (Bouteillan). The reference data of oleuropein content were obtained by the HPLC method. Hundred five samples were analyzed by HPLC and mid-infrared spectroscopy. Samples were randomly divided in a calibration set (73 samples) and in a validation set (32 samples). The spectral data sets were correlated with reference data of oleuropein content by using partial least squares (PLS) regression algorithm. The results showed that the PLS model gave satisfactory model for quantitative prediction of oleuropein content in olive leaf (relative error of prediction = 8.5%). The correlation coefficient was 0.91 and 0.74 for calibration set and validation set, respectively. It can be concluded that mid-infrared spectroscopy constitutes a promising technique for rapid quantification of oleuropein in olive leaf. 相似文献
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Ramirez-Coronel MA Marnet N Kolli VS Roussos S Guyot S Augur C 《Journal of agricultural and food chemistry》2004,52(5):1344-1349
Fresh and 3-day-old coffee pulp of the Arabica variety were analyzed for polyphenol composition followed by characterization by two different methods. The first method consisted in subjecting coffee pulp powder to direct thiolysis. For the second method, coffee pulp was subjected to successive solvent extractions, followed by thiolysis. Quantification of phenolic compounds was then achieved by high-performance liquid chromatography (HPLC) analysis of thiolysis products. Four major classes of polyphenols were identified: flavan-3-ols (monomers and procyanidins), hydroxycinnamic acids, flavonols, and anthocyanidins. Differences in concentration of procyanidins were observed between fresh and 3-day-old coffee pulp. Constitutive units were mainly epicatechin, representing more than 90% of the proanthocyanidin units, with average degrees of polymerization in the range of 3.8-9.1. Monomer to hexamer units of flavan-3-ols from fresh coffee pulp were separated by normal-phase HPLC. Molecular size of oligomeric proanthocyanidins was obtained by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results obtained confirm the presence of oligomers of the flavan-3-ol (-)-epicatechin. 相似文献
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A. Rhouma M. Chettaoui S. Krid H. Elbsir M. Msallem M. A. Triki 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(1):23-33
Forty five descendants from a crossing between olive cultivar Picholine de Languedoc and the local cultivar Meski were evaluated for their susceptibility to olive leaf spot (Fusicladium oleagineum) in field and laboratory studies. Disease incidence varied greatly among the descendants and was correlated with the severity of leaf symptoms. Based on field observations and laboratory tests, the hybrids were classified into five groups: 1) very susceptible to the disease: Meski and 17H, 2) susceptible to the disease : 20J, 8I, 11I, 21I, 14H, 16H, 23H, 6J, 8J and 23J, 3) moderately resistant to the disease : 15I, 16I, 22I, 23I, 8K, 13H, 18H, 22H, 9J, 10J, 11J, 12J, 14J, 16J, 18J, 4) resistant to the disease: 21J, 12I, 13I, 18I, 19I, 9K, 10K, 12H, 15H, 19H, 15J and 22J, and completely resistant to the disease : cultivar Picholine and hybrids 6I and 19I. The analysis of anatomical parameters showed that the polyphenol content and composition were not correlated with disease incidence. However, the cuticle thickness and the trichome density are very important physical parameters that can determine the degree of susceptibility of hybrids to the disease. The assessment method may be useful to screen olive cultivars and hybrids for Fusicladium resistance. 相似文献
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Haifa Kchaou Ajmi Larbi Kamel Gargouri Mohamed Chaieb Fermín Morales Monji Msallem 《Scientia Horticulturae》2010
Changes caused by NaCl-induced salinity on several growth parameters and ions accumulation have been measured in five olive (Olea europaea L.) cultivars (‘Chemlali’, ‘Chetoui’, ‘Koroneiki’, ‘Arbequina I18’, and ‘Arbosana I43’) growing in a greenhouse in nutrient solution pot experiment. One-year-old plants were transplanted to sand–perlite (1:1) culture, and were irrigated with half-strength Hoagland nutrient solution containing NaCl at various levels (0.5, 50, 100 and 200 mM). Salinity induced significant decrease in growth parameters, but to a different extent in each cultivar. Leaf growth and total leaf area per plant were significantly affected by all salinity treatments in all studied cultivars, being ‘Arbequina I18’ the most sensitive cultivar. Leaf drop phenomenon was observed from 60 days after salt application at high salinity treatments, mainly in Arbequina I18. Contrary to leaf area, leaf thickness increased progressively during the experiment. ‘Chemlali’ developed thicker leaves at the two highest salinity treatments when compared to the other cultivars. Na+ and Cl− concentrations were higher in roots than in shoots and leaves in most of the cultivars investigated. The effectiveness of Na+ exclusion mechanism in the roots differed significantly among studied cultivars, working effectively in ‘Chemlali’ (by inhibiting translocation of Na+ to the aerial part) and being much less efficient in ‘Arbequina I18’. Furthermore, leaf abscission can be considered as an additional tolerance mechanism of olive cultivars allowing the elimination of leaves that had accumulated Na+ and Cl− ions. Tolerance to salinity stress was as follows: ‘Chemlali’ > ‘Chetoui’ > ‘Arbosana I43’ > ‘Koroneiki’ > ‘Arbequina I18’. This order of salt tolerance was indicated by lower reduction in plant growth parameters (shoot elongation, trunk diameter, total plant dry weight, internodes length, and total leaf area), the increase of leaf thickness, and by the effectiveness of the exclusion mechanism of Na+ and Cl− in the root system. 相似文献
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Hédia Hannachi Hilary Sommerlatte Catherine Breton Monji Msallem Mohamed El Gazzah Salem Ben El Hadj André Bervillé 《Genetic Resources and Crop Evolution》2009,56(3):393-403
The olive belongs to Olea, a complex genus and to a species with six subspecies. Subsp. europaea includes both the cultivated olive and the oleaster, the wild ancestor of the olive. Little is known on the phenotype of
fruits from subsp. cuspidata. We aimed to compare europaea and cuspidata trees for oil content and composition and to verify whether natural hybrids may exist between the two subspecies. Specimens
were from Kenya and putative natural hybrids between cuspidata and europaea were from Stellenbosch (South Africa). Cultivar and oleaster trees were sampled in France (continental and Corsica), Italy
(Continental and Sardinia) and Tunisia. We have examined the fruit (drupe) structure between subsp. cuspidata and europaea and extracted oil from the pulp, seed and total drupe. Comparison for oil content was made between the cultivated olive and
the oleaster and some cuspidata trees from Kenya. A few of cuspidata individuals from Botanical gardens do not enable phenotyping for oil content and composition. Oil composition analyses were
performed for the main fatty acids to compare the three taxa. We used microsatellite markers at 11 loci to compute genetic
distances between cuspidata, oleaster and cultivar trees and to reveal eventual hybrids. The SSR polymorphisms were huge between the two subspecies and
they revealed that putative hybrids were true hybrids sampled around olive orchards. The whole comparison of oil content shows
that olive cultivars display higher oil content than the subsp. cuspidata and that the oleaster trees are intermediate, whereas for oil composition of the drupe, cuspidata from Kenya shows less oleic acid than europaea. However, the cuspidata trees show seed oil composition similarities with the other two taxa. The discussion deals with possible reasons to explain
the differences and of the possible uses of these taxa for breeding both the olive and brown olive.
H. Hannachi and H. Sommerlatte have equal contribution in the article. 相似文献
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Ajmi Larbi Kamel Gargouri Mohamed Ayadi Ali Ben Dhiab Monji Msallem 《Journal of plant nutrition》2013,36(14):2083-2094
Boron (B) foliar treatments (300 mg L?1 as Solubor DF) were applied at two different dates in 2006 and 2007, prior to flowering and just after fruit set, on olive (Olea europaea L.) trees with no visual symptoms of B deficiency. Leaf B level increased after the first application as compared to control (?B). After July treatment, leaf B levels in ?B and +B treated trees increased when compared to the first sampling date. Foliar B application did not significantly affect vegetative growth in either year. During the first year of study (considered as an “on year”), B application had no significant effect on several phenological characteristics including fruit set, yield oil contents and oil quality. In the second year (“off year”), B sprays improved blooming rate, which increased from 20% in ?B to 30% in + B treated trees, and olive yield, which increased by 27% in response to B. 相似文献
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The main objective of this work was to evaluate if inflorescence analysis could be considered as an alternative to foliar diagnosis in determining the nutritional status of the olive orchards. Olive leaves from ‘Arbequina’ and ‘Chetoui’ cultivars in irrigated and rained systems were sampled within 8 phenological growth stages (dormant inflorescence bud, cluster development, petals whitening, fruit set, fruit development, stone hardening, fruit color break, and fruit ripening) from different sites of Tunisia during 2006 and 2007. Inflorescence samples were taken at petal whitening stage. Results showed that when ignoring the site of experimentation, some significant correlations were obtained between leaves and inflorescence during both years for ‘Arbequina’, at the stone hardening stage: standard date for leaf sampling, for nitrogen (N), phosphorus (P), copper (Cu), zinc (Zn), and manganese (Mn). Further work is required to assess the possibility of using inflorescence analysis to diagnose the nutritional status of olive trees. 相似文献
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