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A flock of 4,500 Cobb broilers inoculated with Newcastle disease vaccine intra-ocular strain B1 type at 10 days of age developed clinical signs of the disease 19 days later; the mortality rate was 71%. Necropsy examinations showed characteristic lesions. Newcastle disease virus was isolated and identified in the allantoic fluid of embryonating chicken eggs by haemagglutination and haemagglutination-inhibition tests. Histopathological examination showed that follicles of the bursa were depleted of lymphocytes, had many large cavities and were being repopulated by newly formed healthy lymphocytes. Both the acute and convalescent serum samples were positive for infectious bursal disease antibodies in agar gel precipitation tests. Haemagglutination inhibition titres of the acute and convalescent sera were 20 to 80 and 80 to 640 respectively. The vaccine failure may be due to either the subclinical bursal disease or the highly pathogenic nature of the wild Newcastle disease virus. 相似文献
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B. O. Odu J. d'A. Hughes † R. Asiedu N. Q. Ng S. A. Shoyinka O. A. Oladiran 《Plant pathology》2004,53(2):141-147
The responses of 24 white yam ( Dioscorea rotundata ) cultivars to mechanical and vector transmission with each of three viruses infecting yams were assessed through enzyme-linked immunosorbent assay (ELISA) and symptom development. The viruses were Dioscorea alata virus (DAV), genus Potyvirus ; Dioscorea alata bacilliform virus (DaBV), genus Badnavirus ; and Cucumber mosaic virus (CMV), genus Cucumovirus . Only TDr 95-128, a landrace cultivar from Nigeria, developed symptoms of infection with CMV and DaBV following mechanical and vector transmission, respectively. PAS-ELISA showed that nine genotypes remained uninfected by DAV and 11 were uninfected by CMV or DaBV. Genotypes TDr 747 and TDr 1640 both showed resistance to all three viruses. 相似文献
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Ezeanyika L.U.S. Obidoa O. Shoyinka V.O. 《Plant foods for human nutrition (Dordrecht, Netherlands)》1999,53(4):351-358
Four week old male Wistar rats were used to study the effects of scopoletin and cyanide on the histopathology of rat brain. The rats were divided into a control and three experimental groups (2–4) and fed rations containing 0.07 g scopoletin/100 g, 0.07g scopoletin + 1.8 mg cyanide/100 g and 1.8 mg cyanide/100 g, respectively. These levels of scopoletin and cyanide corresponded to levels found in a processed cassava diet. The first group was fed the same ration as the others but without scopoletin and cyanide. The rats were fed these rations for twelve months. Rats from each group were sacrificed at the third, sixth, ninth and twelfth months; the relative brain weight of the rats (% of body weight) and histology of their brains were studied. The lipid peroxide levels of the rat brains were also studied at the twelfth month. The results showed that the relative brain weights of the rats fed scopoletin + cyanide were significantly (p<0.05) less than that of the control from the third month. There were no significant changes in the lipid peroxide levels of the rat brains in the various groups. Histological examination of the brains of the rats sugested that scopoletin is involved in the pathogenesis of the neuropathy seen in cassava consuming populations. 相似文献
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Effect of iodine supplementation on thyroid and testicular morphology and function in euthyroid rats
Seventy-five male weaner euthyroid rats, randomly divided into three equal groups were used to evaluate the effect of iodine
supplementation in the diet on growth and spermatogenesis. From the age of six weeks, the rat groups were fed normal diet
containing 0.05 mg iodine/Kg diet (A); normal diet supplemented with 0.5 mg/Kg iodine (B) and normal diet supplemented with
3.0 mg/Kg iodine for a period of 90 days. Thereafter, all three groups were fed the normal diet for another 60 days. Body
weight and feed consumption were determined; morphomeric studies of thyroid glands, testes and epididymes were carried out.
Spermatogenesis was evaluated with epididymal (ESC) and testicular sperm counts (TSC). Increasing iodine intake significantly
(p < 0.05) decreased mean body weight from day 30 of supplementation. Iodine supplementation influenced feed conversion ratio
and efficiency in feed utilization in an inconsistent pattern. Supplementation did not significantly (p > 0.05) alter the
size of thyroid glands, but increased the mean weights of the testes and epididymes to levels significantly (p < 0.05) higher
than values for non-supplemented rats at specific stages of the study, especially at the highest (3 ppm) level of iodine supplementation.
However, supplementation resulted generally in lower sperm counts, which was significant (p < 0.05) in the case of the epididymes.
The results of the study show that iodine supplementation to weaner, non-iodine deficient euthyroid rats at 3ppm not only
retard weight gain but could also reduce fertility by lowering epididymal sperm counts. 相似文献
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I. Reginald Obidike Igwebuike U. Maduabuchi Shoyinka S.V. Olumuyiwa 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):1-5
Potential negative effects of exposure to Nigerian Qua Iboe Brent crude oil on the reproductive system of male rats was investigated. Forty Sprague-Dawley rats were used for the experiment. Exposure to Nigerian Qua Iboe Brent crude oil was achieved via oral administration of increasing doses (0.1, 0.2, and 0.4 ml/rat) every other day for 4 weeks. Cauda epididymal sperm reserves and relative weights of the testes as well as histological features of the testes of rats that received the crude oil treatment were compared to those of control rats. The results described here showed a significant (p < 0.01) dose-dependent reduction in the cauda epididymal sperm reserves of rats that received crude oil treatment relative to the control group. The morphology of testes of the crude oil-exposed rats was characterized by the presence of interstitial exudates, degeneration, and necrosis of spermatogenic and interstitial (Leydig) cells. Findings indicate that exposure of male rats to Nigerian Qua Iboe Brent crude oil may have adversely affected their reproductive systems. This may imply possible reproductive health hazards for animals and humans that may be exposed to this environmental pollutant, especially in areas where oil spillage is a common feature. 相似文献
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