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André Horta Susete Pinteus Celso Alves Nádia Fino Joana Silva Sara Fernandez Américo Rodrigues Rui Pedrosa 《Marine drugs》2014,12(3):1676-1689
Surface-associated marine bacteria are an interesting source of new secondary metabolites. The aim of this study was the isolation and identification of epiphytic bacteria from the marine brown alga, Bifurcaria bifurcata, and the evaluation of the antioxidant and antimicrobial activity of bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol and dichloromethane (1:1) extraction. The antioxidant activity of extracts was performed by quantification of total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and oxygen radical absorbance capacity (ORAC). Antimicrobial activities were evaluated against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Saccharomyces cerevisiae and Candida albicans. A total of 39 Bifurcaria bifurcata-associated bacteria were isolated and 33 were identified as Vibrio sp. (48.72%), Alteromonas sp. (12.82%), Shewanella sp. (12.26%), Serratia sp. (2.56%), Citricoccus sp. (2.56%), Cellulophaga sp. (2.56%), Ruegeria sp. (2.56%) and Staphylococcus sp. (2.56%). Six (15.38%) of the 39 bacteria Bifurcaria bifurcata-associated bacteria presented less than a 90% Basic Local Alignment Search Tool (BLAST) match, and some of those could be new. The highest antioxidant activity and antimicrobial activity (against B. subtilis) was exhibited by strain 16 (Shewanella sp.). Several strains also presented high antimicrobial activity against S. aureus, mainly belonging to Alteromonas sp. and Vibrio sp. There were no positive results against fungi and Gram-negative bacteria. Bifurcaria bifurcata epiphytic bacteria were revealed to be excellent sources of natural antioxidant and antimicrobial compounds. 相似文献
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Daniel Rodrigues Celso Alves André Horta Susete Pinteus Joana Silva Gérald Culioli Olivier P. Thomas Rui Pedrosa 《Marine drugs》2015,13(2):713-726
Cancer and infectious diseases continue to be a major public health problem, and new drugs are necessary. As marine organisms are well known to provide a wide range of original compounds, the aim of this study was to investigate the bioactivity of the main constituents of the cosmopolitan red alga, Sphaerococcus coronopifolius. The structure of several bromoditerpenes was determined by extensive spectroscopic analysis and comparison with literature data. Five molecules were isolated and characterized which include a new brominated diterpene belonging to the rare dactylomelane family and named sphaerodactylomelol (1), along with four already known sphaerane bromoditerpenes (2–5). Antitumor activity was assessed by cytotoxicity and anti-proliferative assays on an in vitro model of human hepatocellular carcinoma (HepG-2 cells). Antimicrobial activity was evaluated against four pathogenic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Compound 4 exhibited the highest antimicrobial activity against S. aureus (IC50 6.35 µM) and compound 5 the highest anti-proliferative activity on HepG-2 cells (IC50 42.9 µM). The new diterpene, sphaerodactylomelol (1), induced inhibition of cell proliferation (IC50 280 µM) and cytotoxicity (IC50 720 µM) on HepG-2 cells and showed antimicrobial activity against S. aureus (IC50 96.3 µM). 相似文献
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Constructed wetlands for the treatment of organic pollutants 总被引:2,自引:1,他引:2
Raimund Haberl Stefano Grego Günter Langergraber Robert H. Kadlec Anna-Rita Cicalini Susete Martins Dias Julio M. Novais Sylvie Aubert Andre Gerth Hartmut Thomas Anja Hebner 《Journal of Soils and Sediments》2003,3(2):109-124
Background Constructed wetlands (wetland treatment systems) are wetlands designed to improve water quality. They use the same processes
that occur in natural wetlands but have the flexibility of being constructed. As in natural wetlands vegetation, soil and
hydrology are the major components. Different soil types and plant species are used in constructed wetlands. Regarding hydrology
surface flow and subsurface flow constructed wetlands are the main types. Subsurface flow constructed wetlands are further
subdivided into horizontal or vertical flow. Many constructed wetlands deal with domestic wastewater where BOD and COD (Biochemical
and Chemical Oxygen Demand respectively) are used as a sum parameter for organic matter. However, also special organic compounds
can be removed.
Objective The objectives are to summarise the state-of-the-art on constructed wetlands for treatment of specific organic compounds,
to the present the lack of knowledge, and to derive future research needs.
Methods Case studies in combination with a literature review are used to summarise the available knowledge on removal processes for
specific organic compounds.
Results and Discussion Case studies are presented for the treatment of wastewaters contaminated with aromatic organic compounds, and sulphonated
anthraquinones, olive mill wastewater, landfill leachate, and groundwater contaminated with hydrocarbons, cyanides, chlorinated
volatile organics, and explosives. In general the removal efficiency for organic contaminants is high in all presented studies.
Conclusion Constructed wetlands are an effective and low cost way to treat water polluted with organic compounds. There is a lack of
knowledge on the detailed removal pathways for most of the contaminants. Removal rates as well as optimal plant species are
substance-specific, and also typically not available. If a constructed wetland provides different environmental conditions
and uses different plant species the treatment efficiency can be improved.
Recommendations and Outlook There is a great need to lighten the black box ‘constructed wetland’ to obtain performance data for both microbial activity
and the contribution of the plants to the overall removal process. Also genetic modified plants should be considered to enhance
the treatment performance of constructed wetlands for specific compounds. 相似文献
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Patrícia Susano Joana Silva Celso Alves Alice Martins Helena Gaspar Susete Pinteus Teresa Mouga Mrcia Ines Goettert
eljko Petrovski Luís B. Branco Rui Pedrosa 《Marine drugs》2021,19(3)
The ever-increasing interest in keeping a young appearance and healthy skin has leveraged the skincare industry. This, coupled together with the increased concern regarding the safety of synthetic products, has boosted the demand for new and safer natural ingredients. Accordingly, the aim of this study was to evaluate the dermatological potential of the brown seaweed Carpomitra costata. The antioxidant, anti-enzymatic, antimicrobial, photoprotective and anti-inflammatory properties of five C. costata fractions (F1–F5) were evaluated. The ethyl acetate fraction (F3) demonstrated the most promising results, with the best ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (EC50 of 140.1 µg/mL) and the capacity to reduce reactive oxygen species (ROS) production promoted by UVA and UVB radiation in 3T3 cells, revealing its antioxidant and photoprotective potential. This fraction also exhibited the highest anti-enzymatic capacity, inhibiting the activities of collagenase, elastase and tyrosinase (IC50 of 7.2, 4.8 and 85.9 µg/mL, respectively). Moreover, F3 showed anti-inflammatory potential, reducing TNF-α and IL-6 release induced by LPS treatment in RAW 264.7 cells. These bioactivities may be related to the presence of phenolic compounds, such as phlorotannins, as demonstrated by NMR analysis. The results highlight the potential of C. costata as a source of bioactive ingredients for further dermatological applications. 相似文献
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Sea cucumber Holothuria forskali,a new resource for aquaculture? Reproductive biology and nutraceutical approach
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Rita Santos Simaura Dias Susete Pinteus Joana Silva Celso Alves Carla Tecelão Rui Pedrosa Ana Pombo 《Aquaculture Research》2016,47(7):2307-2323
Sea cucumbers are highly marketable as a food product due to their nutritional value. Also, it has been suggested that sea cucumbers possess a wide range of bioactive compounds that can be used in the pharmaceutical industry. In this study, the reproductive biology of Holothuria forskali was performed by evaluating the gonadosomatic index (GI) and histological analyses of the gonadal tubules. The biotechnological potential was assessed through the evaluation of the antioxidant, antimicrobial and antitumor potential. Finally, the fatty acid profile was also evaluated. These three subjects were chosen to increase the interest and to focus the economic potential of this species rearing, predicting that it can be sold in Europe or export to Asia to be used for human consumption or for the pharmaceutical industry. The GI and the histological analysis of the gonadal tubules revealed that the range from February to March corresponds to the peak of gonads maturation. Furthermore, the methanolic fraction revealed the highest antimicrobial potential against Candida albicans with an IC50 of 233.2 mg mL?1. Also, this fraction presented the highest cytotoxic and anti‐proliferative activities through the method for measuring cell proliferation method in both cell lines, with an IC50 of 238.2 and 396.0 mg mL?1 for MCF‐7 cells respectively and 260.3 and 218.7 mg mL?1 for HepG‐2 cells respectively. Regarding the fatty acid profile, the total fat content was 4.83% and the highest values were obtained for palmitic acid (9.96%), stearic acid (11.23%), eicosapentaenoic acid (10.49%) and arachidonic acid (20.36%). 相似文献
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Joana Alves Helena Gaspar Joana Silva Celso Alves Alice Martins Fernando Teodoro Patrícia Susano Susete Pinteus Rui Pedrosa 《Marine drugs》2021,19(11)
Inflammation is a double-edged sword, as it can have both protective effects and harmful consequences, which, combined with oxidative stress (OS), can lead to the development of deathly chronic inflammatory conditions. Over the years, research has evidenced the potential of marine sponges as a source of effective anti-inflammatory therapeutic agents. Within this framework, the purpose of this study was to evaluate the antioxidant and the anti-inflammatory potential of the marine sponge Cliona celata. For this purpose, their organic extracts (C1–C5) and fractions were evaluated concerning their radical scavenging activity through 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), and anti-inflammatory activity through a (lipopolysaccharides (LPS)-induced inflammation on RAW 264.7 cells) model. Compounds present in the two most active fractions (F5 and F13) of C4 were tentatively identified by gas chromatography coupled to mass spectrometry (GC-MS). Even though samples displayed low antioxidant activity, they presented a high anti-inflammatory capacity in the studied cellular inflammatory model when compared to the anti-inflammatory standard, dexamethasone. GC-MS analysis led to the identification of n-hexadecanoic acid, cis-9-hexadecenal, and 13-octadecenal in fraction F5, while two major compounds, octadecanoic acid and cholesterol, were identified in fraction F13. The developed studies demonstrated the high anti-inflammatory activity of the marine sponge C. celata extracts and fractions, highlighting its potential for further therapeutic applications. 相似文献
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