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1.
Toscana virus (TOSV), West Nile virus (WNV) and tickborne encephalitis virus (TBEV) are among major viral pathogens causing febrile disease and meningitis/encephalitis. The impact of these viruses was investigated at a referral centre in Ankara Province, Central Anatolia in 2012, where previous reports suggested virus circulation but with scarce information on clinical cases and vector activity. Serum and/or cerebrospinal fluid samples from 94 individuals were evaluated, in addition to field‐collected arthropod specimens that included 767 sandflies and 239 mosquitoes. Viral nucleic acids in clinical samples and arthropods were sought via specific and generic nested/real‐time PCRs, and antibody responses in clinical samples were investigated via commercial indirect immunofluorescence tests (IIFTs) and virus neutralization. A WNV antigen assay was also employed for mosquitoes. WNV neuroinvasive disease has been identified in a 63‐year‐old male via RNA detection, and the WNV strain was characterized as lineage 1. TOSV infections were diagnosed in six individuals (6.3%) via RNA or IgM detection. Partial sequences in a 23‐year‐old female, presented with fever and transient pancytopenia, were characterized as TOSV genotype A. Febrile disease with arthralgia and/or peripheral cranial nerve involvement was noted in cases with TOSV infections. Previous WNV and TOSV exposures have been observed in 5.3% and 2.1% of the subjects, respectively. No confirmed TBEV exposure could be identified. Morphological identification of the field‐collected mosquitoes revealed Culex pipiens sensu lato (74.4%), Anopheles maculipennis (20.9%), An. claviger (2.1%) and others. Sandfly species were determined as Phlebotomus papatasi (36.2%), P. halepensis (27.3%), P. major s. l. (19.3%), P. sergenti (8.9%), P. perfiliewi (4.4%), P. simici (2.6%) and others. Viral infections in arthropods could not be demonstrated. TOSV genotype A and WNV lineage 1 activity have been demonstrated as well as serologically proven exposure in patients. Presence of sandfly and mosquito species capable of virus transmission has also been revealed.  相似文献   
2.
The biosorption of Ce(III) from aqueous solution by citric acid-modified Pinus brutia leaf powder was studied in a batch system as a function of initial pH, temperature, initial concentration of adsorbate, and contact time. Central composite design method was used in the experiments. Thermodynamic parameters such as standard enthalpy (ΔH 0), entropy (ΔS 0), and free energy (ΔG 0) were calculated, and the results indicated that biosorption was exothermic. The biosorption of Ce(III) on modified Pinus brutia leaf powder was investigated by Freundlich, Langmuir, and Dubinin-Radushkevich (D-R) isotherms. The results show that Ce(III) adsorption can be explained by Langmuir isotherm model, and monolayer capacity was found as 62.1?mg/g. The results suggested that the modification process enhances the biosorption capacity of the adsorbent, and modified Pinus brutia leaf powder may find promising applications for the recovery of Ce(III) from aqueous effluents.  相似文献   
3.
Macrolides are considered to be one of the safest anti-infective groups in clinical use, with severe adverse reactions being rare. However, there are limited data about their embryotoxicity and teratogenicity. We aimed to investigate and compare the effects of these agents on embryonic growth and development. Rat embryos were cultured in vitro for 48 h in rat serum. Whole rat serum was used as a culture medium for the control group while different concentrations of spiramycin and azithromycin (1.25-6.25 microg/ml), and clarithromycin (2.5-30 microg/ml) were added to rat serum for the experimental groups. Dose-dependent effects of macrolides on embryonic developmental parameters were compared using morphological methods. Embryos were evaluated for the presence of any malformations. After morphological examination of the embryos, total DNA was extracted from the cells using standard procedures to determine fragmentation of nuclear DNA of embryonic cells. When compared with the control embryos, the macrolides significantly decreased all growth and developmental parameters dose dependently. While clarithromycin was found to cause more developmental toxicity than spiramycin and azithromycin, azitromycin was determined to have more teratogenicity potential. Compared with controls, there was no difference regarding the fragmentation of nuclear DNA of all the agents used. According to these results, when the toxic and teratogenic potential of the used agents compared, because of the lower toxic and teratogenic effects observed with spiramycin, this agent may be preferred for parturients.  相似文献   
4.
Abstract

This three-year study (2003–2005) aimed to improve the yield and quality of pastures growing naturally that are colonized by naturally occurring vegetation without agricultural input under hazelnut (Corylus sp.) orchards in the middle and eastern Black Sea regions of Turkey. There were eight treatments: 1) control; 2) fertilizer only (triple superphosphate 44% and calcium ammonium nitrate 26%) (80kg ha?1 P and 60 kg ha?1 N in Samsun; 100 kg ha?1 P and 80 kg ha?1 N in Ordu; 100 kg ha?1 P and 40 kg ha?1 N in Giresun); 3) lime only (calcium carbonate 94%) (3.0 t ha?1 lime in Samsun; 4.5 t ha?1 lime in Ordu and Giresun); 4) early cut only; 5) soil aeration only; 6) fertilizer+lime; 7) fertilizer+lime+early cut; 8) fertilizer+lime+soil aeration, laid out in a randomized complete block design with four replicates at each location. The highest dry matter (DM) yield of kg ha?1 and crude protein content (%) was obtained from the treatments that included fertilizer. There was no difference in DM production between any of the combination treatments that involved fertilizer and the fertilizer alone treatment. Only lime and aeration applications also increased yield compared to control, but not as much as did any treatment including fertilizer. Crude protein content of the pasture ranged from 13.3 to 18.1% across locations. Nitrogen and phosphorus fertilizer are recommended to improve DM yields and herbage quality for pastures under hazelnut orchards.  相似文献   
5.
Salinity and Orobanche or Phelipanche spp. infection are important crop stress factors in agricultural areas. In this study, we investigated the effect of salt stress on Phelipanche ramosa seed germination and its attachment onto Arabidopsis thaliana roots. We also evaluated the effect of both stresses on the expression of genes regulated by abiotic and biotic stresses. According to our results, high concentration of NaCl delayed P. ramosa seed germination in the presence of a strigolactone analogue (GR24). A similar pattern was observed in the presence of A. thaliana plants. Furthermore, we found that salt‐treated A. thaliana seedlings were more sensitive to P. ramosa attachment compared with the untreated plants, indicating that there was a positive correlation between salt sensitivity and the ability of P. ramosa to infect A. thaliana plants. At the molecular level, a synergystic effect of both salt and P. ramosa stresses was observed on the cold‐regulated (COR) gene expression profile of treated A. thaliana seedlings. Our data clarify the interaction between parasitic plants and their hosts under abiotic stress conditions.  相似文献   
6.
This study explored the influence of 10 annual forage legumes belonging to the Lathyrus and Vicia genera on wet aggregate stability (WAS) and dispersion ratio (DR) indices of a clay soil. Five Lathyrus and five Vicia species were sown in autumn. Seed‐to‐seed and row‐to‐row distance was maintained at 5 cm and 20 cm, respectively. The experiment was planned in a randomized block design with three replications. After 90 d following seed harvest, soil cores were collected from two depths (0–15, 15–30 cm) in each plot and WAS and DR were determined. Annual forage legumes increased WAS of the soil but decreased the DR index. The WAS and DR values were affected at level of p < 0.001 by genus, species, and soil depth. Values of WAS and DR of the control plots without plant on the average were found to be 44.5% and 9.3% for 0–15 cm, and 41.2% and 10.1% for 15–30 cm, respectively. For 0–15 cm depth, the highest WAS (77.7%) and the lowest DR (6.4%) values were found in L. sphaericus L. (wild) plots. For 15–30 cm depth, the highest WAS value (62.6%) was obtained in L. annuus L. (wild) plots and the lowest DR value (6.7%) was in L. sativus L. (Gurbuz‐2001) plots.  相似文献   
7.
Tower yarders have recently been introduced to forestry in Turkey. Clarification of the productivity and cost of logging using the tower yarder is often requested because the cost for machinery is a significant factor in all calculations concerning mechanized operations. Machines are often extremely expensive compared with the low cost of labor in developing regions. In this study, a new logging system using a tower yarder was compared with a conventional system using a stationary yarder in terms of productivity and cost. The research was conducted in the northeast of Turkey, in 1989 and 1992. The productivity of the tower yarder and the stationary yarder was found to be 5.655 m3/h and 5.002 m3/h, respectively. Harvesting cost was analyzed based on observed productivity. The harvesting costs of the tower yarder and the stationary yarder were found to be 47,410 TL/m3 and 17,553 TL/m3, respectively. With the tower yarder, the machine cost reached 93.1 % of the harvesting cost while the machine cost using the stationary yarder reached 71.1%. A part of this paper was orally presented at the 3rd Annual Meeting of the Japan Forest Engineering Society (1996).  相似文献   
8.
Selamectin, a novel avermectin compound, was evaluated for its efficacy against naturally occurring infestations of Psoroptes cuniculi and Sarcoptes scabiei. A total of 42 New Zealand rabbits with psoroptic mange and 37 Angora rabbits with sarcoptic mange were used in the present study. On day 0, infested rabbits were treated topically with either selamectin at minimum dose of 6 mg kg(-1) (6-18 mg kg(-1) for New Zealand rabbits, n = 31 and 10-12 mg kg(-1) for Angora rabbits, n = 23) or vehicle only (control groups, n = 11 for New Zealand rabbits, n = 14 for Angora rabbits). The efficacy of selamectin was assessed both clinically and parasitologically by the presence or absence of viable mites. Rabbits were scraped for sarcoptic mites on days 7, 14, 28, 42 and 56 and had otoscopeic and/or microscopic examination for the detection of Psoroptes mites on days 7, 14, 42 and 56. Fisher's exact test was used to assess differences between the vehicle and selamectin treatment in the number of rabbits without mites (cure rates) on each assessment date. It was found that significantly fewer selamectin-treated rabbits had mites detected on skin scrapings (for S. scabiei) or otoscopeic and/or microscopic examination (for P. cuniculi) (P < 0.01) than the vehicle group. Results of the present study suggest that selamectin is effective against naturally infestations of P. cuniculi and S. scabiei in rabbits.  相似文献   
9.
This, partly retrospective study, was designed to determine the seroprevalence of Schmallenberg virus (SBV), a new Orthobunyavirus first reported in Germany in late 2011, in domestic ruminants from the Middle Black Sea, West, and Southeast regions of Turkey. An indirect enzyme-linked immunosorbent assay was used to screen serum samples collected from slaughterhouse animals between 2006 and 2013. The overall seroprevalence was 335/1,362 (24.5 %) with 325/816 (39.8 %), 5/307 (1.6 %), 3/109 (2.8 %), and 2/130 (1.5 %) recorded in cattle, sheep, goats, and Anatolian water buffalo, respectively. This is the first study to demonstrate the presence of antibodies to SBV in Turkish ruminants; it indicates that cattle are more susceptible to infection than sheep, goats, or buffalo and that exposure of domestic ruminants to SBV in Turkey may have occurred up to 5 years prior to the first recorded outbreak of the disease in 2011.  相似文献   
10.
A feeding experiment was conducted to study the response of rainbow trout juveniles fed different levels of lupin meal in diets for rainbow trout juveniles. Very limited information is available on the relationship between dietary lupin meal in rainbow trout health status. Therefore, this study aimed to determine the effects of lupin meal inclusion levels (0%, 15%, 30%, 45%, and 60%) on growth performance and health status of rainbow trout juveniles. The experimental diets (LM0, LM15, LM30, LM45, and LM60) were formulated iso‐nitrogenous (41% crude protein) and iso‐calorific (18% crude lipid). The fish were fed twice a day. As a result, the best growth performance was observed in fish fed with LM15 and LM30 diets. No significant differences were detected among experimental groups in terms of body compositions. The haematological values showed significantly (p < 0.05) lower heamatocrit and mean cellular volume (MCV) in the group of LM60 compared with the other groups. For the other haematological parameters such as haemoglobin, red blood cell and mean cellular haemoglobin studied in the present study no significant differences were observed (p < 0.05). The lupin meal included groups showed significant reduction in total protein (TPROT), triglyceride (TROG), cholesterol (CHOL), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) compared with the control group (p < 0.05). The inclusion of lupin meal did not cause any changes in glucose (GLU), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) between the treatment groups (p < 0.05). In conclusion, lupin meal might be used in rainbow trout diets up to 30% without any malnutrition effect on growth performance, haemotological and serum biochemical parameters.  相似文献   
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