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排序方式: 共有280条查询结果,搜索用时 31 毫秒
1.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
2.
3.
Shotaro NISHIMURA Hitomi SHIMODA Ichiro OSHIMA Yoshitaka ONO Kaoru OKANO Akira ISHIBASHI Shoji TABATA Hisao IWAMOTO 《Animal Science Journal》2005,76(6):575-579
Alpha‐melanocyte stimulating hormone (α‐MSH) is one of the main regulators for melanocytes, and the adenohypophysis is one of the major tissues for the synthesis of this hormone. The Silky fowl is a characteristic breed of chicken with hyperpigmentation throughout the body. The involvement of the adenohypophysis in the hyperpigmentation of this breed is not known. In the present study, the proportion of melanocyte stimulating hormone‐immunopositive cells (MSH cells) in the adenohypophysis was immunocytochemically compared between Silky, Red Cornish × New Hampshire (RN) crossbred and Japanese bantam cockerels at 15 weeks of age. After the body and gland were weighed, the adenohypophyseal cells were enzymatically dispersed and immunostained for α‐MSH, and the immunopositive MSH cells were counted. The weights of the body and adenohypophysis were heaviest in the RN crossbred, followed by the Silky and lightest in the bantam cockerels. In contrast, the ratio between adenohypophysis and bodyweight was much larger in the Silky and the bantam than in the RN crossbreed (P < 0.05). The population of MSH cells in the adenohypophysis was larger in the Silky (14.3%) than in the RN crossbreed (8.0%) and the bantam (8.1%) cockerels (P < 0.05). From these results, it was concluded that prepubertal Silky cockerel have numerous MSH cells in the adenohypophysis suggesting a relationship to hyperpigmentation. 相似文献
4.
Production of inter-section hybrids between Primula filchnerae and P. sinensis through ovule culture
Inter-section hybrids were obtained in the reciprocal crosses between Primula filchnerae (2n = 2x = 24) of Sect. Pinnatae and P. sinensis ‘Fanfare’ (2n = 2x = 24) of Sect. Auganthus by rescuing ovules on half-strength (1/2) Murashige and Skoog's (MS) medium supplemented with 50 g l−1 sucrose, 2.5 g l−1 gellan gum, 0.1 mg l−1 α-naphthaleneacetic acid (NAA), 0.1 mg l−1 6-benzyladenine (BA) and 50 mg l−1 gibberellic acid (GA3). In ovule culture, germination occurred with radicle elongation but no plumule was observed. The radicle kept on the initial medium showed root proliferation with callus formation. When the calluses were transferred to (1/2)MS media containing 30 g l−1 sucrose and 3 g l−1 gellan gum, without plant growth regulators (PGRs) or with 1 mg l−1 zeatin and 0.1 mg l−1 NAA, plantlets were regenerated. The plants thus obtained were confirmed to be hybrids through flow cytometry (FCM) and random amplified polymorphic DNA (RAPD) analyses. The hybrid obtained when P. filchnerae was used as the maternal parent was diploid, whereas hexaploid hybrid was obtained when using P. sinensis as the maternal parent. The hexaploid hybrid might be produced through chromosome doubling of a triploid originated from the fertilization of P. sinensis with unreduced pollen of P. filchnerae. 相似文献
5.
Yoshimasa Sugiura Kohji Matsuda Takashi Okamoto Makoto Kakinuma Hideomi Amano 《Fisheries Science》2008,74(1):180-186
To investigate the anti-allergic effects of the brown alga Eisenia arborea. A strain of Brown Norway rats know to strongly respond to immunoglobulin E (IgE) were used as an allergy model animal. The
rats were immunized with ovalbumin by oral administration. The levels of serum IgE and histamine were suppressed in the rats
fed a diet supplemented with dried E. arborea powder. As for the cytokine pattern, the interferon-γ production in the spleens and mesenteric lymph nodes (MLN) was enhanced,
and the interleukin-4 (IL-4) production in the spleens and/or IL-10 production in the spleens and MLN were suppressed. These
results, together with the change in the Th1/Th2 balance, indicate that the rats fed with E. arborea became more anti-allergic, suggesting that E. arborea might possess anti-allergic effects. 相似文献
6.
Spatially distributed morphological characteristics of macropores in forest soils of Hitachi Ohta Experimental Watershed, Japan 总被引:1,自引:0,他引:1
Shoji Noguchi Yoshio Tsuboyama Roy C. Sidle Ikuhiro Hosoda 《Journal of Forest Research》1997,2(4):207-215
Morphological characteristics of macropores in forest soil profiles were investigated at Hitachi Ohta Experimental Watershed
in Japan. Nine individual profiles at different locations (various spatial scales in a catchment) and twenty profiles at one
site (a small spatial scale) were excavated to the bedrock to investigate density, origin, diameter, direction, and gradient
of macropores. Macropore densities in a soil profile ranged from 3.5 to 29.1 per m and from 5.4 to 75.1 per m2, respectively. Subsurface erosion, root channels, and interactions between subsurface erosion and root channels accounted
for 36.9, 36.5, and 19.0%, of the described macropores. The mean macropore diameter in organic-rich soil layer (17–20 mm)
was larger than in the B horizon (11–14 mm) at both spatial scales. The dominant gradients of all macropores in the organic-rich
soil layer and B horizon were at negative oblique angles. Approximately 90% of the macropores in the organic-rich soil layer
and approximately 80% of the macropores in the B horizon fell within the range between −50 and 50 degree planar direction.
Subsurface flow and root systems are believed to play important roles in determining the morphological characteristics of
macropores. These characteristics appear to have variable influences in different soil horizons rather than at different spatial
scales.
A part of this paper was presented at the 103th (1992) and 105th (1994) Annual Meetings of Japanese Forestry Society. 相似文献
7.
Shoji Ohga 《Journal of Wood Science》1999,45(4):337-342
The influence of substrate water potential () on the growth and fruiting of three genotypes of shiitake (Lentinula edodes) was investigated. A slight reduction of (–0.5MPa) stimulated mycelial and colony growth on liquid, agar, and sawdust-based substrates.L. edodes has been found to grow well at a around –0.5 MPa, which corresponds to a moisture content around 55%. A small decrease in at the final vegetative growth phase had positive effects on flush quantity. The substrate was significantly affected by the interaction between genotypes and spawn run time. The of well-colonized mature substrate was –0.7MPa before and –4.OMPa after the fruiting. The rose again to –0.7MPa during rapid absorbance of water by soaking, and this rise was repeated during the second and third flushes. It is suggested that the water-holding capacity of a substrate is related to culture maturity. Excellent water-providing capacity (higher) is expected in the substrate of well-matured cultures with a high density of mycelial colonization.Part of this paper was presented at the 46th annual meeting of the Japan Wood Research Society, Kumamoto, April 1996 相似文献
8.
The shape of the flexural vibration wave of wooden beams at the first mode was detected using the transfer function. The
dynamic modulus of elasticity (MOE) of beam sections of differing quality was estimated from the ratio of the curvature of
the wave shape in this section to that of a clear beam. The results were as follows: (1) If a section with a lower dynamic
MOE was introduced into a clear wooden beam, the curvature of the wave shape in that section became higher. (2) The ratio
of the MOE and the reciprocal of the curvature ratio were highly correlated. (3) The MOE of a defect could be estimated, and
the position of the defect could be determined accurately by examining the curvature of the flexural vibration wave shapes.
Received: March 22, 2002 / Accepted: May 15, 2002
Part of this report was presented at the 50th Annual Meeting of the Japan Wood Research Society, Kyoto, April 2000. This article
is translated from the Japanese version, which was published in Mokuzai Gakkaishi 47(5), 2001
Correspondence to:Y. Ishimaru 相似文献
9.
Anna Kakehashi Akihiro Hagiwara Norio Imai Min Wei Shoji Fukushima Hideki Wanibuchi 《Journal of toxicologic pathology》2015,28(1):27-32
In the present study, in continuation of our previous experiment in order to investigate the mode of action (MOA) of ethyl tertiary-butyl ether (ETBE) hepatotumorigenicity in rats, we aimed to examine alterations in cell proliferation, that are induced by short-term administration of ETBE. F344 rats were administered ETBE at doses of 0, and 1,000 mg/kg body weight twice a day by gavage for 3, 10, 17 and 28 days. It was found that the previously observed significant increase of P450 total content and hydroxyl radical levels after 7 days of ETBE administration, and 8-OHdG formation at day 14, accompanied by accumulation of CYP2B1/2B2, CYP3A1/3A2, CYP2C6, CYP2E1 and CYP1A1 and downregulation of DNA oxoguanine glycosylase 1, was preceded by induction of cell proliferation at day 3. Furthermore, we observed an increase in regenerative cell proliferation as a result of ETBE treatment at day 28, followed by induction of cell cycle arrest and apoptosis by day 14. These results indicated that short-term administration of ETBE led to a significant early increase in cell proliferation activity associated with induction of oxidative stress, and to a regenerative cell proliferation as an adaptive response, which could contribute to the hepatotumorigenicity of ETBE in rats. 相似文献
10.
Distribution of free L-cysteine and glutathione in seaweeds 总被引:1,自引:0,他引:1