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1.
Asuka Kumagai Kyoko Kawauchi Kiyohiko Andoh Shinichi Hatama 《Journal of veterinary diagnostic investigation》2021,33(1):162
Bovine respiratory syncytial virus (BRSV) is an etiologic agent of bovine respiratory disease. The rapid evolutionary rate of BRSV contributes to genetic and antigenic heterogeneity of field strains and causes occasional vaccine failure. We conducted molecular epidemiologic characterization of BRSV circulating in Japan to obtain genetic information for vaccine-based disease control. Phylogenetic analysis of G and F gene sequences revealed that all of the isolated Japanese BRSV strains clustered in the same genetic subgroup, which was distinct from the 9 known groups. We assigned the Japanese group to subgenotype X. The Japanese isolates formed 2 temporal clusters: isolates from 2003 to 2005 clustered in lineage A; isolates from 2017 to 2019 formed lineage B. The alignment of the deduced amino acid sequences of the G gene revealed that the central hydrophobic region responsible for viral antigenicity is conserved in all of the isolates; unique amino acid mutations were found mainly in mucin-like regions. Our results suggest that BRSV has evolved uniquely in Japan to form the new subgenotype X; the antigenic homogeneity of the viruses within this group is inferred. 相似文献
2.
An attempt was made to account for quantitatively measured herbicidal performance of foliage-applied 5-hydroxy-3-methyl-2–oxo-imidazolidine-1-carboxamide derivatives by their photosynthesis-inhibiting activity and systemicity. Photosynthesis-inhibiting activity was estimated from the increase of chlorophyll fluorescence intensity in Chlorella vulgaris Beijer cells measured by a microplate scanner, and systemicity was also evaluated by computer-aided chlorophyll fluorescence imaging. The highest herbicidal performance was recorded for N-cyclohexyl-5-hydroxy-3-methyl-2-oxo-imidazo～idine-l-carboxamide, a compound with the second strongest photosynthesis-inhibiting activity and intermediate systemicity. Though neither photosynthesis-inhibiting activity nor systemicity showed significant correlation with the actual herbicidal performance in simple regression analyses, a high predictability was found for a multiple regression on both parameters as two independent variables, suggesting that these two factors work cooperatively in the field performance. 相似文献
3.
F.?Ishiguri S.?Maruyama K.?Takahashi Z.?Abe S.?Yokota M.?Andoh N.?YoshizawaEmail author 《Journal of Wood Science》2003,49(2):135-139
Sugi green logs with red or black heartwood were smoke-heated, and the changes in the color of the heartwood after ultraviolet
(UV) (λ = 365 nm) radiation exposure were then observed. After UV radiation exposure, the redness and yellowness increased in both
the red and black heartwoods, whereas the brightness decreased. In the black heartwood, the resulting color turned from yellowish
white to reddish brown. Reddening in black heartwood after exposure to a combination of smoke heating and UV radiation is
thought to be due to a decrease in brightness and an increase in both redness and yellowness. However, the degree of change
in heartwood color by UV radiation exposure was not greatly affected by smoke-heating treatments of various lengths. When
methanol extracts were fractionated and exposed to UV radiation, the yellowness increased in the n-hexane-soluble portion and the redness increased in the acetone-soluble fractions from the n-hexane-insoluble portion. These results suggest that the n-hexane-soluble fraction contains the substances that allow heartwood color to change to yellow after UV radiation exposure,
and the acetone-soluble-fraction from the n-hexane-insoluble portion contains the substances that allow it to change to red.
Received: November 14, 2001 / Accepted: June 3, 2002
Acknowledgment This research was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of
Science.
This study was presented in part at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001
Correspondence to:N. Yoshizawa 相似文献
4.
Hotta A Zhang GQ Andoh M Yamaguchi T Fukushi H Hirai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1289-1291
Monoclonal antibodies (MAbs) to major antigens of Coxiella burnetii were produced. Some of the MAbs to a 62-kDa protein antigen, peptidoglycan protein complex and lipopolysaccharide (LPS) O-chains reacted with other bacteria whereas none of the MAbs to outer membrane proteins and LPS outer-core did. The LPS outer-core and OMPs may be useful antigens for specifically detecting antibodies to C. burnetii. 相似文献
5.
Fisheries Science - The activity of proteases may be lost during microquantification because of their nonspecific binding to tubes and pipette tips. In this study, the effects of blocking reagents... 相似文献
6.
Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses 总被引：1，自引：0，他引：1
Maeda K Yasumoto S Tsuruda A Andoh K Kai K Otoi T Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):989-991
In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK-Tcl3, and EHV-3 also propagated in FHK-Tcl3. These results indicated that this novel cell line, FHK-Tcl3, can be used for isolation and propagation of equine herpesviruses. 相似文献
7.
Ishihara K Takahashi R Andoh M Ueno H Muramatsu Y Tamura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):117-120
Campylobacter was isolated from retail meat samples collected during the fiscal year 2009 in Japan. The higher percentages of contamination of chicken products were observed from June (39.3%) to November (83.3%). However, the highest number of human campylobacteriosis cases was reported in June in the Infectious Agents Surveillance Report. The chicken isolates with distinct clusters IVb and I, based on the restriction fragment length polymorphism of the flaA gene, were predominantly obtained during the periods between April and November 2009 and between February and March 2010, respectively. Extensive monitoring of Campylobacter contamination in chickens produced in various places is needed to analyse the seasonal variations between contamination of the meat products and the number of human cases with campylobacteriosis. 相似文献
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9.
T. Nakanishi M. Atarashi‐Andoh J. Koarashi Y. Saito‐Kokubu K. Hirai 《European Journal of Soil Science》2012,63(4):495-500
Although considerable research has been conducted on the importance of recent litter compared with older soil organic matter as sources of dissolved organic carbon (DOC) in forest soils, a more thorough evaluation of this mechanism is necessary. We studied water‐extractable organic carbon (WEOC) in a soil profile under a cool‐temperate beech forest by analysing the isotopic composition (13C and 14C) of WEOC and its fractions after separation on a DAX‐8 resin. With depth, WEOC became more enriched in 13C, which reflects the increasing proportion of the hydrophilic, isotopically heavier fraction. The 14C content in WEOC and its fractions decreased with depth, paralleling the 14C trend in soil organic matter (SOM). These results indicate a dynamic equilibrium of WEOC and soil organic carbon. The dominant process maintaining the WEOC pool in the mineral soil appears to be the microbial release of water‐soluble compounds from the SOM, which alters in time‐scales of decades to centuries. 相似文献
10.
Masuda T Yonemori S Oyama Y Takeda Y Tanaka T Andoh T Shinohara A Nakata M 《Journal of agricultural and food chemistry》1999,47(4):1749-1754
The antioxidant activity of the methanolic extracts of the leaves of 39 plant species was examined. These leaves were collected from the plants growing on subtropical seashores. The activity was evaluated by three kinds of assay methods, which included the DPPH radical scavenging assay, linoleic acid oxidation assay, and oxidative cell death assay. Two extracts from Excoecaria agallocha and Terminalia catappa showed remarkably potent activity in all assay systems. The HPLC analysis of the extracts indicated the presence of the same antioxidant and isolation work for the compound identified ellagic acid. The isolated ellagic acid showed strong antioxidant activity in the assay systems used. 相似文献