Proteomic analysis of intestinal mucosa responses to Salmonella enterica serovar typhimurium in naturally infected pig     

C. Arce  C. Lucena  A. Moreno  J.J. Garrido 《Comparative immunology, microbiology and infectious diseases》2014

Salmonella enterica serovar typhimurium (S. typhimurium) is one of the most frequent Salmonella serotypes isolated from European pigs. Despite the advances in understanding the mechanisms involved in host–pathogen interactions and host cell responses to S. typhimurium, the global change that occurs in naturally exposed populations has been poorly characterized. Here, we present a proteomics study on intestinal mucosa of pigs naturally infected with S. typhimurium, in order to better understand the pathogenesis of salmonellosis and the pathways which might be affected after infection. Samples were analyzed by 2D-DIGE and 44 different proteins exhibited statistically significant differences. The data set was analyzed by employing the Ingenuity Pathway Analysis and the physiological function most significantly perturbed were immunological and infectious disease, cellular assembly and organization and metabolism. The pathways implicated in the porcine immune response to S. typhimurium were gluconeogenesis and Rho GDI/RhoA signaling, and our results suggest that keratins and the intermediate filaments could play an important role in the damage of the mucosa and in the success of infection. The role of these findings in salmonellosis has been discussed, as well as the importance of analyzing naturally infected animals to have a complete picture of the infection. Also, we compared the results found in this work with those obtained in a similar study using experimentally infected animals.  相似文献   

Temporal changes in seabird assemblage structure and trait diversity in the Rapa Nui (Easter Island) multiple-use marine protected area     

Paula Plaza  Juan Serratosa  Joao B. Gusmao  David C. Duffy  Paulina Arce  Guillermo Luna-Jorquera 《水产资源保护:海洋与淡水生态系统》2021,31(2):378-388

1. For Rapa Nui (Easter Island) and its largest islet, Motu Nui, the change of the species assemblage over time was analysed, and a trait-based approach to evaluate the potential losses in seabird function across the past centuries was applied. At a finer scale, the seasonal changes in seabird species composition in the current seabird assemblage was assessed to better understand the dynamics of the long-term inferred patterns.
2. For Rapa Nui, the composition of the seabird assemblage between the prehistorical, historical, and current time has changed significantly. The most critical change, probably associated with human colonization, was observed between prehistoric and current times. The current diminished number of nesting seabird species was probably the result of local extirpation without evidence of colonization by new species.
3. For Motu Nui, changes in species composition were also followed by changes in trait structure, which were smaller than observed in Rapa Nui. This is probably due to the presence of a relatively high number of related species (i.e. Procellariids) with high similarities in their foraging behaviour.
4. The nesting seabird assemblages in Rapa Nui and Motu Nui differ in exposure to risk; thus, conservation strategies applied to the islands should be planned on a fine spatial scale. For Rapa Nui, which is an urban wildlife area with several invasive species and a low number of remaining native seabird species, management should focus on fencing and pets control. For Motu Nui, management should instead focus on the establishment of quarantine and other biosecurity tools to avoid both the entry and proliferation of new invasive species.

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Enhanced resistance to bacterial infection byErwinia carotovora subsp.atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes     

Patricio Arce  Mauricio Moreno  Mónica Gutierrez  Marlene Gebauer  Paola Dell’Orto  Hebert Torres  Ivette Acuña  Pauline Oliger  Alejandro Venegas  Xavier Jordana  Julio Kalazich  Loreto Holuigue 《American Journal of Potato Research》1999,76(3):169-177

Blackleg and soft rot diseases, caused by the bacteriumErwinia carotovora, are among the diseases that cause important losses in culture and storage of potato. In this paper, we introduced bacterial resistance into potato, via genes encoding for proteins with antibacterial activity. For this purpose, potato clones were transformed either with the gene encoding the acidic attacin protein fromHyalophora cecropia, or with the gene encoding the cecropin analog peptide SB37. These clones were evaluated for soft rot and blackleg resistance, after inoculation with the bacterial strainErwinia carotovora subsp.atroseptica T7. Results reported in this paper indicate that a considerable percentage of the potato clones (15–22%) showed increased resistance to bacterial infection, revealed by reduced severity of blackleg or soft rot symptoms. Expression of the transgenes was demonstrated in some of the clones by Northern blot analysis. This is the first report indicating that expression of the gene encoding for an attacin protein and for the cecropin SB-37 peptide in transgenic potato confers increased resistance to bacterial infection.  相似文献   

A multiplex RT-PCR assay for the rapid and differential diagnosis of classical swine fever and other pestivirus infections   总被引：2，自引：0，他引：2  

Heidy Díaz de Arce  Lester J. Prez  Maria T. Frías  Rosa Rosell  Joan Tarradas  Jos I. Núez  Llilianne Ganges 《Veterinary microbiology》2009,139(3-4):245-252

Classical swine fever is a highly contagious viral disease causing severe economic losses in pig production almost worldwide. All pestivirus species can infect pigs, therefore accurate and rapid pestivirus detection and differentiation is of great importance to assure control measures in swine farming. Here we describe the development and evaluation of a novel multiplex, highly sensitive and specific RT-PCR for the simultaneous detection and rapid differentiation between CSFV and other pestivirus infections in swine. The universal and differential detection was based on primers designed to amplify a fragment of the 5′ non-coding genome region for the detection of pestiviruses and a fragment of the NS5B gene for the detection of classical swine fever virus. The assay proved to be specific when different pestivirus strains from swine and ruminants were evaluated. The analytical sensitivity was estimated to be as little as 0.89 TCID₅₀. The assay analysis of 30 tissue homogenate samples from naturally infected and non-CSF infected animals and 40 standard serum samples evaluated as part of two European Inter-laboratory Comparison Tests conducted by the European Community Reference Laboratory, Hanover, Germany proved that the multiplex RT-PCR method provides a rapid, highly sensitive, and cost-effective laboratory diagnosis for classical swine fever and other pestivirus infections in swine.  相似文献   

First report of porcine circovirus type 2 infections in Cuba     

Lester J. Pérez  Heidy Díaz de Arce  Maria I. Percedo  Patricia Domínguez  Maria T. Frías 《Research in veterinary science》2010,88(3):528-530

To obtain information about the porcine circovirus type 2 (PCV2) infection status of pigs in Cuba and the probable association of PCV2 with other porcine viruses, tissue samples collected from ill pigs were evaluated using polymerase chain reaction (PCR). The PCR analysis showed that 67.7% of the samples (23/34) from seven swine herds of six different geographic regions were detected to be positive for PCV2. Ten of the 23 PCV2 positive samples (43.5%) shown a concurrent infection with porcine parvovirus (PPV) and 17 of 23 PCV2 positive samples (73.9%) exhibited a concomitant infection with classical swine fever virus (CSFV). This study is the first report of PCV2 infecting pigs with different clinical conditions in Cuban swine herds and provides evidence of PCV2 co-infection with PPV and CSFV in the field.  相似文献   

Simulated keratometry and central corneal thickness measured in cats by autokeratometer,ultrasonic pachymeter,and Galilei G6™     

Leticia de Andrade Ramos  Cláudia Valéria Seullner Brandão  Carlos Guillermo Arce  Laura Micheline  Micaella Gordon Gandolfi  Carlos Roberto Padovani 《Veterinary ophthalmology》2023,26(2):128-134

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Sugar fragmentation in the maillard reaction cascade: formation of short-chain carboxylic acids by a new oxidative alpha-dicarbonyl cleavage pathway     

Davídek T  Robert F  Devaud S  Vera FA  Blank I 《Journal of agricultural and food chemistry》2006,54(18):6677-6684

The formation of short-chain carboxylic acids was studied in Maillard model systems (90 degrees C, pH 6-10) with emphasis on the role of oxygen and water. The total amount of acetic acid formed did not depend on the reaction atmosphere. In the presence of labeled dioxygen or water (18O2, H2 17O), labeled oxygen was partially incorporated into acetic acid. Thermal treatment of 1-deoxy-d-erythro-2,3-hexodiulose (1) and 3-deoxy-d-erythro-hexos-2-ulose in the presence of 17O-enriched water under alkaline conditions led to acetic and formic acid, respectively, as indicated by 17O NMR spectroscopy. The suggested mechanism involves an oxidative alpha-dicarbonyl cleavage leading to an intermediary mixed acid anhydride that releases the acids, e.g., acetic and erythronic acid, from 1. Similarly, glyceric and lactic acids were formed from 1-deoxy-3,4-hexodiuloses, corroborated by complementary analytical techniques. This paper provides for the first time evidence for the direct formation of acids from C6-alpha-dicarbonyls by an oxidative mechanism and incorporation of a 17OH group into the carboxylic moiety. The experimental data obtained support the coexistence of at least two newly described reaction mechanisms leading to carboxylic acids, i.e., (i) a hydrolytic beta-dicarbonyl cleavage as a major pathway and (ii) an alternative minor pathway via oxidative alpha-dicarbonyl cleavage induced by oxidizing species.  相似文献   

Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes   总被引：5，自引：0，他引：5  

Arce C  Moreno A  Millán Y  Martín de las Mulas J  Llanes D 《Veterinary immunology and immunopathology》2002,86(1-2):31-41

In this study, the effect of two oligodeoxynucleotide (ODN) sequences 5'GCT-AGA-CGT-TAG-CGT-3' (CpG-ODN) and 5'-GCT-AGA-GCT-TAG-GCT-3' (GpC-ODN) on the antigen-specific antibody and cellular immune response after intramuscular immunizations with OVA was analyzed in pigs. Pigs immunized with OVA supplemented with these ODNs showed a significantly enhanced primary antibody response in comparison with the control group which received OVA without ODN. This enhanced primary antibody response appeared ODN-sequence-independent as similar effects were seen in both ODN-groups. The OVA-specific antibody titers obtained after a single injection of antigen combined with either of both ODNs were as high as the titers in the control group after two injections. Furthermore, the ODN-supplemented animals showed significantly higher OVA-specific IgA antibodies in their saliva and nasal secretions at some time points after the first immunization. Proliferation assays showed that CpG- as well as GpC-ODN significantly enhanced the antigen-specific as well as the mitogen-induced proliferation in different lymphoid tissues. Furthermore, 48h after the third immunization the CpG-group showed a significantly decreased IL-6 mRNA expression in cells of the local draining lymph node but no significant difference in TGF-beta (Th3-like) and IL-10 (Th2-like). The ODN injected animals showed the tendency to have higher IFN-gamma (Th1-like) mRNA-expression in comparison with the control group. To our knowledge, these are the first in vivo studies in pigs, which demonstrate the appropriateness of CpG-ODN as immunostimulating adjuvants in vaccines for farm animals.  相似文献   

Generation of roasted notes based on 2-acetyl-2-thiazoline and its precursor, 2-(1-hydroxyethyl)-4,5-dihydrothiazole, by combined bio and thermal approaches     

Bel Rhlid R  Fleury Y  Blank I  Fay LB  Welti DH  Arce Vera F  Juillerat MA 《Journal of agricultural and food chemistry》2002,50(8):2350-2355

Roasted notes contribute to the flavor of thermally processed foods such as meat and bread. 2-Acetyl-2-thiazoline is one of the key volatile compounds responsible for the roasted and popcorn-like aroma character. We report here on the biogeneration of flavoring preparations with intense roasted notes, which are characterized by a high content of 2-acetyl-2-thiazoline. These flavoring preparations were obtained by fermentation of cysteamine, ethyl-L-lactate, and D-glucose with baker's yeast. The precursor of 2-acetyl-2-thiazoline, 2-(1-hydroxyethyl)-4,5-dihydrothiazole, was prepared under mild conditions by microbial reduction of the carbonyl group of 2-acetyl-2-thiazoline using baker's yeast as biocatalyst. The addition of 2-(1-hydroxyethyl)-4,5-dihydrothiazole as aroma precursor to pizza dough resulted in an increase of the roasted note.  相似文献   

Effect of soil depth on acid properties of humic substances extracted from an ombrotrophic peat bog in northwest Spain     

D. Gondar  R. López  S. Fiol  J. M. Antelo  & F. Arce 《European Journal of Soil Science》2005,56(6):793-801

The most southerly ombrotrophic peat bogs in Europe are in Galicia (northwest Spain). The humic matter in them originates from chemical processes in anaerobic conditions. We studied the acid properties of fulvic acids and humic acids isolated from two peat horizons of an ombrotrophic peat bog by potentiometric titration. Solutions containing 25, 50 and 100 mg l⁻¹ of each humic substance were titrated at ionic strengths 0.005 m , 0.01 m and 0.1 m (with KNO₃ as the inert electrolyte). Charge curves were analysed with a Donnan model to determine the intrinsic proton binding parameters. The concentration of the humic substance affected the charge curves more significantly at pH exceeding 6, and tended to disappear at greater concentrations. The proton binding conditional constants decreased with increasing ionic strength, this effect being more significant in the carboxylic groups with less affinity for protons. The proton binding constant of the carboxyl groups in a fulvic acid was one order of magnitude less than the value for the corresponding humic acid, whereas for the phenolic groups the values for both fractions were similar. The total content of acid groups was approximately 2 mol kg⁻¹ greater in the fulvic fraction than in the humic fraction. Both humic fractions from the lower horizon contained more acid groups than those from the upper horizon, mainly because the content of carboxyl groups increases with soil depth. Therefore, the humic substances in the lower horizon of the peat will be more negatively charged, which will affect their solubility and the binding of metal ions.  相似文献