SxtA and sxtG Gene Expression and Toxin Production in the Mediterranean Alexandrium minutum (Dinophyceae)     

Federico Perini  Luca Galluzzi  Carmela Dell’Aversano  Emma Dello Iacovo  Luciana Tartaglione  Fabio Ricci  Martino Forino  Patrizia Ciminiello  Antonella Penna 《Marine drugs》2014,12(10):5258-5276

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Recycling of Two-Phase Olive-Mill Cake “Alperujo” by Co-composting with Animal Manures     

Concepción Paredes  Maria Dolores Pérez-Murcia  Aurelia Pérez-Espinosa  M. Ángeles Bustamante  Joaquín Moreno-Caselles 《Communications in Soil Science and Plant Analysis》2015,46(4):238-247

The use of poultry manure or goat/sheep manure in the co-composting of the two-phase olive-mill cake “alperujo” (ALP) with olive leaf (OL) is compared by studying organic-matter mineralization and humification processes during composting and the characteristics of the end products. For this, two different piles (P1 and P2) were prepared using ALP with OL mixed with poultry manure (PM) and goat/sheep manure (GSM), respectively, and composted by the turned windrow composting system. Throughout the composting process, a number of parameters were monitored, such as temperature, pH, electrical conductivity (EC), organic matter (OM), OM losses, total organic carbon (C_org), total nitrogen (N_t), C_org/N_t ratio, and the germination index (GI). In both piles, the temperature exceeded 55 °C for more than 2 weeks, which ensured maximum pathogen reduction. Organic-matter losses followed a first-order kinetic equation in both piles. The final composts presented a stabilized OM and absence of phytotoxins, as observed in the evolution and final values of the C_org/N_t ratio (C_org/N_t < 20) and the germination index (GI > 50 percent). Therefore, composting can be considered as an efficient treatment to recycle this type of waste, obtaining composts with suitable properties that can be safely used in agriculture.  相似文献   

A comparative study of the precipitation acidity in two cities in Cantabria (Spain) with different degrees of industrialization     

Aurelia Bonet  Nieves Diaz-Caneja  Isabel Gutierrez  Antonio Martínez  Eugenio Villar 《Water, air, and soil pollution》1988,38(1-2):181-188

A study was carried out on the pH values obtained from bulk (wet and dry) precipitation samples collected simultaneously in two cities in Cantabria (Spain), Santander and Torrelavega over a period of a year. It was observed that the annual weighted average values for the pH of precipitation, 4.42 and 4.21, respectively, differ significantly although the difference is less than that existing between their pollution levels. The difference is greater when total annual H⁺ depositions are compared. The study also demonstrated the strong influence of long-range transport effect on acidity and to a lesser degree the effect of mid-range transport.  相似文献   

Production,characterization, and effects on tomato of humic acid-like polymerin metal derivatives from olive oil mill waste waters     

Capasso R  De Martino A  Cristinzio G 《Journal of agricultural and food chemistry》2002,50(14):4018-4024

The dark polymeric organic fraction rich in potassium recovered from olive oil mill waste waters (OMWW) and named polymerin and the potassium salified deglycosylated polymerin derivative (K-SDpolymerin) were easily transformed into their metal derivatives by saturation with various metals, including Na, Cu, Zn, Mn, Fe, and Al. Saturated metal polymerins were characterized by diffuse reflectance infrared Fourier transform spectroscopy and atomic absorption spectrometry. Tests on tomato plants of the various polymerins showed that only the soluble polymerin, K-SDpolymerin, and the insoluble Mn-SDpolymerin were significantly toxic. The toxic effects of OMWW on tomato at the original concentration and diluted 1:10 were much stronger than those of any polymerin. The possible exploitation of polymerins as bioamendments and/or metal biointegrators as a functon of their phytotoxic effects, their humic acid-like nature, and their richness in macro- and micronutrient metals is also discussed.  相似文献   

A note on diseases of mink.     

P E Martino  J J Martino  J A Villar 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(3):227-230

The paper describes in brief the findings in 5,616 autopsies of adult mink, performed at the Institute of Pathology-CIC, La Plata, during the last five years. Nearly all these carcasses, coming from the most important ranches, were examined bacteriologically and histopathologically. Observations about the incidence of different causes of death, its distribution annually and prophylactic measures were reported. It concludes that Aleutian disease is the most important cause of death and produces severe economic losses.  相似文献   

Duplex polymerase chain reaction assay to screen for feline herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats     

Marsilio F  Di Martino B  Aguzzi I  Meridiani I 《Veterinary research communications》2004,28(Z1):295-298

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Bovine herpesvirus type 1 (BHV-1) up-regulates telomerase activity in MDBK cells     

Pagnini U  De Martino L  Montagnaro S  Diodato A  Longo M  Pacelli F  Pisanelli G  Iovane G 《Veterinary microbiology》2006,113(3-4):231-236

The proliferative capacity of mammalian cells is regulated by telomerase, an enzyme uniquely specialised for telomeric DNA synthesis. The critical role of telomerase activation in tumor progression and maintenance has been well established in studies of cancer and of oncogenic transformation in cell culture. Experimental data suggest that telomerase activation has an important role in normal somatic cells, and that failure to activate sufficient telomerase also promotes disease. Evidence regarding the role of telomerase in the pathogenesis of several viruses including human immunodeficiency virus has led to an increased interest in the role of telomerase activity in other virus infections. In this research we evaluated the telomerase modulating activity of Bovine herpesvirus 1 (BHV-1) in MDBK cells. MDBK cells were infected at different multiplicity of infection with BHV-1 Cooper strain and telomerase activity at different times post-infection was measured by the TRAP assay. Our data indicate that BHV-1 significantly up-regulates telomerase activity at 3 and 6h post-infection decreasing after the 24h post-infection. Our data, showed that the effect was mediated by an immediate-early or early viral gene, and use of the protein translation inhibitor cycloheximide confirmed that an immediate early gene is primarily responsible.  相似文献   

Reproductive performance of anoestrous high-producing dairy cows improved by adding equine chorionic gonadotrophin to a progesterone-based oestrous synchronizing protocol     

Garcia-Ispierto I  López-Helguera I  Martino A  López-Gatius F 《Reproduction in domestic animals》2012,47(5):752-758

This study sought to improve the reproductive performance of anoestrous high-producing dairy cows by including equine chorionic gonadotrophin (eCG) after progesterone-releasing intravaginal device (PRID) removal. In Experiment I, 806 cows at 51-57 days post-partum were randomly assigned to a PRID (treated with PRID), PRID-500 (treated with PRID plus 500 IU of eCG) or PRID-750 (treated with PRID plus 750 IU of eCG) group. In Experiment II, 422 cows showing a long anoestrus period (animals with no oestrus signs nor luteal tissue 35 days before treatment) were randomly assigned to the PRID, PRID-500 or PRID-750 groups. The dependent variables considered in binary logistic regression analyses for both experiments were the rates of oestrus, ovulation and conception after treatment, the cumulative conception rate on Day 120 post-partum and pregnancy loss. In Experiment I, interaction between treatment and season showed a significant effect on the oestrous response. Thus, during the warm season, PRID group cows were 8.9 times more likely to express oestrus than the remaining cows. Moreover, inseminated cows with two or more corpora lutea 8-14 days after treatment were more likely to become pregnant (by a factor of 2.4) than cows with a single corpus luteum. Finally, cows without luteal structures treated with PRID were 0.4 less likely to be pregnant on Day 120 post-partum, compared with the remaining cows. In Experiment II, cows in the PRID group treated during the warm or cool season were less likely to exhibit oestrus (by a factor of 0.06 or 0.2, respectively) or ovulate (by a factor of 0.004 or 0.14, respectively) than the remaining cows. In conclusion, in anoestrous cows in both experiments, the addition of eCG to the use of an intravaginal progesterone device to induce oestrus was beneficial. The recommended dose of eCG is 500 IU.  相似文献   

Biological indicators for evaluating soil quality improvement in a soil degraded by erosion processes     

Onet  Aurelia  Dincă  Lucian C.  Grenni  Paola  Laslo  Vasile  Teusdea  Alin C.  Vasile  Diana L.  Enescu  Raluca E.  Crisan  Vlad E. 《Journal of Soils and Sediments》2019,19(5):2393-2404

Journal of Soils and Sediments - Erosion is one of the main soil degradation problems. It diminishes soil biological activity and therefore its quality. The aim of the study was to evaluate if the...  相似文献   

Optimization of color and antioxidant activity of peach and nectarine puree: scale-up study from pilot to industrial plant     

Lavelli V  Pompei C  Casadei MA 《Journal of agricultural and food chemistry》2008,56(16):7091-7099

The effects of an innovative process for the manufacture of peach and nectarine purees on the main quality indices, namely, color, consistency, carotenoid and phenolic content, and antioxidant activity, were studied using a peach cultivar that is optimal for nectar processing (cv. Redhaven) and peach and nectarine varieties that undergo a faster browning degradation. The innovative process, operating the pulping/finishing step at room temperature, was compared to the traditional process of hot pulping/finishing. The study comprised initial trials on a pilot plant scale and scaling up to industrial production of the puree and nectar. The quality of products was analyzed at the time of production and as a function of storage of both the puree and the nectar. With respect to the traditional process, the new process, scaled up to industrial levels, improved the color of peach and nectarine products (by increasing the L* value and decreasing the a* value), whatever the variety studied; maintained almost the same levels of carotenoids, hydroxycinnamates, flavan-3-ols, and flavonols; and reduced the level of cyanidin 3-O-glucoside. The presence of cyanidin 3-O-glucoside was correlated to an unstable and undesirable red hue of the products (even if its concentration was very low in all products), and the decreased level obtained by the innovative process was considered to be positive. On the basis of these results, new technology can be proposed for the processing of fruit varieties that are not suitable for puree production using traditional technology. This opens up two possibilities: (a) utilization of fresh market fruit surplus and (b) processing of selected fruit varieties that are rich in antioxidants but have a high browning potential, such as the Stark Red Gold nectarine. Furthermore, as the positive impact of the new technology is optimal at the beginning of storage, it is particularly suitable for fruit-based products with a short shelf life.  相似文献