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Peru Gopal BISWAS Yuma OHARI Uday Kumar MOHANTA Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):666
We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future. 相似文献
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An experiment in seed morphology and seed germination techniques of Albizia procera was carried out in the nursery of the Forestry and Wood Technology Discipline,Khulna University,Bangladesh in order to discover the source variation in seeds and pre-sowing treatment effects on seed germination.Mature seeds of A.procera were collected from healthy trees in home garden plantations from five different districts in Bangladesh and treated with four pre-sowing treatments,i.e.,control,immersion in cold water (4°C for 24 h)and immersion in hot water(80°C for 10 min and 100°C for 1 min).The average length,width and thickness of seeds were calculated as 0.502±0.485,0.420±0.060 and 0.191±0.118 cm,respectively.Germination was conducted in poly-bags with a mixture of topsoil and cow dung in a ratio of 3:1.The results revealed that pre-sowing treatments affected the rate of germination of seeds,which significantly increased the germination percentages of seeds in hot water treatments compared with those in control (60.60%)and the cold water treatment(4°C for 24 h,63.53%).The highest germination success was 82.07%in the treatment of immersion in hot water(80°C)for 10 min,followed by 79.00%in immersion in hot water(100°C)for 1 min.Germination started 4 to 6 days after seed sowing and completed in a period of 22 to 25 days in all treatments.ANOVAs showed statistically significant differences(p0.05)in seed germination starting dates,closing dates,germination percentages and rates of germination among treatments,but no significant differences in seed germination starting dates,closing dates,germination period,germination percentages and rates of germination among the seed sources.The study also revealed that the interaction between seed source variation and treatment effect significantly differed in seed germination starting dates,closing dates,germination percentages and rates of germination. The hot water(100°C for 1 min)treatment is recommended for seed germination of A.procera in rural Bangladesh. 相似文献
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ABSTRACT: To study the influence of different photoperiod cycles on the metabolic rate and energy loss of fed and unfed adult tilapia Oreochromis niloticus (bodyweight 102–107 g) at 28°C, four photoperiod cycles (i.e. 3L : 3D, 6L : 6D, 12L : 12D, and 24L : 24D) were applied. A computer-operated respirometer with a closed tank was used so as to prevent water from condensing from the air or evaporating into the air. A photoperiod-mediated metabolic cycle was demonstrated during the routine state in which the metabolic rate was higher during the light phase compared with during the dark phase for all photoperiods. The combined effects of photoperiod and feeding episodes acted as a strong Zeitgeber (cue or synchronizer) for synchronizing the daily rhythm in fed fish. Fish exposed to short photoperiod cycles showed a higher metabolic rate and energy loss compared with those exposed to longer photoperiod cycles. Mean oxygen consumption in the fed and unfed fish were 295.7 mg/kg per h and 149.8 mg/kg per h, respectively, during the 3L : 3D period; 286.5 mg/kg per h and 143.3 mg/kg per h during the 6L : 6D period; 262.2 mg/kg per h and 130.3 mg/kg per h during the 12L : 12D period; and 238.3 mg/kg per h and 120.4 mg/kg per h during the 24L : 24D period. The highest post-prandial increase in energy loss was recorded during the 3L : 3D period (56.2 kJ/kg per day), followed by 55.1 kJ/kg per day during the 6L : 6D period, 50.7 kJ/kg per day during the 12L : 12D period, and 45.4 kJ/kg per day during the 24L : 24D period. The study's results demonstrated that the fish conserve energy when raised under longer photoperiod cycles. 相似文献
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Daniel D. SAURETTE Asim BISWAS Richard J. HECK Adam W. GILLESPIE Aaron A. BERG 《土壤圈》2024,34(3):530-539
In digital soil mapping (DSM), a fundamental assumption is that the spatial variability of the target variable can be explained by the predictors or environmental covariates. Strategies to adequately sample the predictors have been well documented, with the conditioned Latin hypercube sampling (cLHS) algorithm receiving the most attention in the DSM community. Despite advances in sampling design, a critical gap remains in determining the number of samples required for DSM projects. We propose a simple workflow and function coded in R language to determine the minimum sample size for the cLHS algorithm based on histograms of the predictor variables using the Freedman-Diaconis rule for determining optimal bin width. Data preprocessing was included to correct for multimodal and non-normally distributed data, as these can affect sample size determination from the histogram. Based on a user-selected quantile range (QR) for the sample plan, the densities of the histogram bins at the upper and lower bounds of the QR were used as a scaling factor to determine minimum sample size. This technique was applied to a field-scale set of environmental covariates for a well-sampled agricultural study site near Guelph, Ontario, Canada, and tested across a range of QRs. The results showed increasing minimum sample size with an increase in the QR selected. Minimum sample size increased from 44 to 83 when the QR increased from 50% to 95% and then increased exponentially to 194 for the 99% QR. This technique provides an estimate of minimum sample size that can be used as an input to the cLHS algorithm. 相似文献
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Peru Gopal BISWAS Yuma OHARI Uday Kumar MOHANTA Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):226
The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis. 相似文献
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Effect of different photoperiod cycles on metabolic rate and energy loss of both
fed and unfed young tilapia Oreochromis niloticus: Part I 总被引：1，自引：0，他引：1
ABSTRACT: The influence of different photoperiod cycles (3L : 3D, 6L : 6D, 12L : 12D, and 24L : 24D) on the metabolic rate and energy loss of either fed or unfed young tilapia Oreochromis niloticus (bodyweight 8.6–9.5 g) was investigated at 28°C. A computer-operated respirometer with a closed tank was used to prevent water from evaporating into the air or condensing from the air. The photoperiods acted as strong Zeitgeber (i.e., cue or synchronizer) during the experiments with either fed or unfed fish. A photoperiod-mediated metabolic cycle was demonstrated in fed and unfed fish in which oxygen consumption was higher during the light phase compared with during the dark phase for all photoperiods. Mean oxygen consumption during the 3L : 3D, 6L : 6D, 12L : 12D, and 24L : 24D periods for fed and unfed fish were 685.06 mg/kg per h and 299.33 mg/kg per h; 658.52 mg/kg per h and 284.80 mg/kg per h; 591.09 mg/kg per h and 249.62 mg/kg per h; and 500.64 mg/kg per h and 239.14 mg/kg per h, respectively. The highest post-prandial increase in energy loss was recorded during the 3L : 3D period (145.88 kJ/kg per day), followed by 141.19 kJ/kg per day during the 6L : 6D, 128.70 kJ/kg per day during the 12L : 12D, and 99.92 kJ/kg per day during the 24L : 24D periods. The results suggest that higher energy conservation would be achieved if fish are exposed to longer rather than shorter photoperiod cycles. 相似文献
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Sampling Designs for Validating Digital Soil Maps: A Review 总被引：1，自引：0，他引：1
Sampling design (SD) plays a crucial role in providing reliable input for digital soil mapping (DSM) and increasing its efficiency. Sampling design, with a predetermined sample size and consideration of budget and spatial variability, is a selection procedure for identifying a set of sample locations spread over a geographical space or with a good feature space coverage. A good feature space coverage ensures accurate estimation of regression parameters, while spatial coverage contributes to effective spatial interpolation. First, we review several statistical and geometric SDs that mainly optimize the sampling pattern in a geographical space and illustrate the strengths and weaknesses of these SDs by considering spatial coverage, simplicity, accuracy, and efficiency. Furthermore, Latin hypercube sampling, which obtains a full representation of multivariate distribution in geographical space, is described in detail for its development, improvement, and application. In addition, we discuss the fuzzy k-means sampling, response surface sampling, and Kennard-Stone sampling, which optimize sampling patterns in a feature space. We then discuss some practical applications that are mainly addressed by the conditioned Latin hypercube sampling with the flexibility and feasibility of adding multiple optimization criteria. We also discuss different methods of validation, an important stage of DSM, and conclude that an independent dataset selected from the probability sampling is superior for its free model assumptions. For future work, we recommend:1) exploring SDs with both good spatial coverage and feature space coverage; 2) uncovering the real impacts of an SD on the integral DSM procedure; and 3) testing the feasibility and contribution of SDs in three-dimensional (3D) DSM with variability for multiple layers. 相似文献
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Dipta DEY Tanzila Ismail EMA Partha BISWAS Sharmin AKTAR Shoeba ISLAM Urmi Rahman RINIK Mahmudul FIROZ Shahlaa Zernaz AHMED Salauddin AL AZAD Ahsab RAHMAN Sadia AFRIN Rezwan Ahmed MAHEDI Md. Nasir Uddin BADAL 《农业科学与工程前沿(英文版)》2021,8(4):603
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