In vitro bactericidal activity of 465–470 nm blue light phototherapy and aminolevulinic acid on Staphylococcus pseudintermedius          下载免费PDF全文

Seulgi Bae  Taeho Oh 《Veterinary dermatology》2018,29(4):296-e102

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Genetic Diversity and Population Structure in the Heavily Exploited Korean Rockfish,Sebastes schlegeli,in Korea     

Hyesuck An  Mi‐Jung Kim  Kwangjae Park  Keechae Cho  Bongseong Bae  Jaewoo Kim  Jeong‐In Myeong 《Journal of the World Aquaculture Society》2012,43(1):73-83

The Korean rockfish, Sebastes schlegeli, is a valuable and intensively exploited species in Korea. We discuss the genetic diversity and genetic structure of four Korean rockfish populations using eight microsatellite loci. In total, 161 different alleles from 138 individuals were observed. Average allele number per locus ranged from 2.5 to 23 and allelic richness varied from 13.38 to 14.63 within a population. Despite a long history of stocking practices, we found very high levels of polymorphism (mean heterozygosity = 0.810), which is comparable to other congeneric species. No significant difference in genetic diversity and molecular genetic variance (F_ST and R_ST) was observed among four local samples (P > 0.1). Little indication of contemporary inbreeding (F_IS= 0.051) or population structure (K = 1) was detected. This absence of differentiation may reflect high levels of gene flow along the coast of Korea. Our study demonstrates that rockfish in Korea should be managed as a single unit. Currently, the species does not appear to be genetically threatened, but the potential for a rapid loss of genetic diversity remains. This information on the genetic characteristics of Korean rockfish populations has important implications for fisheries management and conservation efforts, and will aid in the sustainable exploitation of the fishing resources and the preservation of biodiversity.  相似文献   

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction     

Kim DG  Bae JY  Hong GE  Min MK  Kim JK  Kong IS 《Journal of fish diseases》2008,31(9):639-647

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.  相似文献   

Compensatory growth and body composition of juvenile black rockfish <Emphasis Type="Italic">Sebastes schlegeli</Emphasis> following feed deprivation   总被引：1，自引：0，他引：1  

Sung-Yong Oh  Choong Hwan Noh  Rae-Seon Kang  Chong-Kwan Kim  Sung Hwoan Cho  Jae-Yoon Jo 《Fisheries Science》2008,74(4):846-852

ABSTRACT:   Compensatory growth, feeding rate, feed efficiency and chemical composition of juvenile black rockfish (mean weight 1.43 g) were investigated for 35 days after a 14-day feed deprivation treatment under four feeding conditions: one group continuously fed (control) and the other three groups fasted for 5 days (F5), 10 days (F10) and 14 days (F14). All fasted fish were re-fed from day 15. Only F5 achieved the same body weight as the control, indicating that complete compensation occurred in F5. The specific growth rate (SGR) of F5 was the highest at day 21 and then decreased thereafter, showing higher values than the control at days 21, 28 and 42. In contrast, although SGRs of F10 and F14 were higher than that of the control during the whole refeeding period except day 21, they did not catch up the control in body mass, indicating that only partial compensation occurred in F10 and F14. The feeding rate (FR) of all groups except F14 changed in a pattern similar to SGR (Spearman's rank correlation, r _s > 0.9), suggesting that SGR varied depending on FR. Similar feeding efficiencies (FEs) were found in the four groups and they did not vary significantly during the whole refeeding period, suggesting that FE was not the factor affecting SGR. At day 14, the ratios of lipid to lean body mass in F10 and F14 were lower than those in the control and F5, and there was no difference between the control and F5. At day 49, however, only F14 showed a lower value than the other three groups, and there was no difference among the three groups. These results indicate that juvenile black rockfish fasted for 5–14 days can exhibit compensatory growth after refeeding, but timing and degree vary depending on the duration of feed deprivation.  相似文献   

Differential expression of a poplar copper chaperone gene in response to various abiotic stresses     

Lee H  Lee JS  Bae EK  Choi YI  Noh EW 《Tree physiology》2005,25(4):395-401

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Review of the <Emphasis Type="Italic">Eilema</Emphasis> group of lichen moths from South Korea,with description of one new species (Lepidoptera: Erebidae: Arctiinae: Lithosiini)     

Ulziijargal Bayarsaikhan  Young-Don Ju  Yang-Seop Bae 《林业研究》2016,27(2):407-417

This review details the Korean species of the Eilema group in the subfamily Arctiinae of Erebidae: 19 species in five genera (Manulea, Collita, Katha, Dolgoma, and Wittia), a new species (Collita hwacheonensis Bayarsaikhan & Bae, sp. nov.), and an unrecorded species [C. vetusta aegrota (Butler 1877)]. All species are diagnosed, and figures of the adults and genitalia and a key to species based on male genital structure of Eilema group in South Korea are included.  相似文献   

Impact of Buckwheat Flavonoids on In Vitro Starch Digestibility and Noodle‐Making Properties     

In Young Bae  Ah Sah Choi  Hyeon Gyu Lee 《Cereal Chemistry》2016,93(3):299-305

The effects of various buckwheat materials (buckwheat flour [BF], dietary fiber extract [DE], flavonoids extract [FE], and rutin‐enhanced flavonoids extract [REFE]) on starch digestibility and noodle‐making properties were evaluated. When FE and REFE were incorporated into noodles, the amount of rapidly digestible starch and the predicted glycemic index (pGI) were reduced. However, BF and DE did not significantly decrease the pGI value of noodles. When assessing noodle properties, hardness was increased with increasing content of buckwheat materials, whereas other texture parameters were not significantly affected by buckwheat addition. All noodles were similar in regard to water absorption and swelling index, but cooking loss was slightly increased in FE and REFE noodles. FE and REFE demonstrated higher flavonoid stability during noodle making and, additionally, were more effective at reducing starch digestibility than BF and DE. REFE, specifically, does not generate quercetin (the cause of a bitter taste), and, therefore, REFE was effective in suppressing the hydrolysis of starch in the noodles, lowering the pGI.  相似文献   

Comparative functional anatomy of hindlimb muscles and bones with reference to aquatic adaptation of the sea otter     

Kent MORI  Satoshi SUZUKI  Daisuke KOYABU  Junpei KIMURA  Sung-Yong HAN  Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):571-578

Although the sea otter (Enhydra lutris) is a complete aquatic species, spending its entire life in the ocean, it has been considered morphologically to be a semi-aquatic animal. This study aimed to clarify the unique hindlimb morphology and functional adaptations of E. lutris in comparison to other Mustelidae species. We compared muscle mass and bone measurements of five Mustelidae species: the sea otter, Eurasian river otter (Lutra lutra), American mink (Neovison vison), Japanese weasel (Mustela itatsi) and Siberian weasel (M. sibirica). In comparison with the other 4 species, E. lutris possessed significantly larger gluteus, popliteus and peroneus muscles, but smaller adductor and ischiopubic muscles. The popliteus muscle may act as a medial rotator of the crus, and the peroneus muscle may act as an abductor of the fifth toe and/or the pronator of the foot. The bundles of the gluteus superficialis muscle of E. lutris were fused with those of the tensor fasciae latae muscle and gluteofemoralis muscles, and they may play a role in femur abduction. These results suggest that E. lutris uses the abducted femur, medially rotated crus, eversion of the ankle and abducted fifth digit or extended interdigital web as a powerful propulsion generator. Therefore, we conclude that E. lutris is a complete aquatic animal, possessing differences in the proportions of the hindlimb muscles compared with those in other semi-aquatic and terrestrial mustelids.  相似文献   

Protective effect of butylated hydroxylanisole against hydrogen peroxide-induced apoptosis in primary cultured mouse hepatocytes     

Geun Hye Hwang  Yu Jin Jeon  Ho Jae Han  Soo Hyun Park  Kyoung Min Baek  Woochul Chang  Joong Sun Kim  Lark Kyun Kim  You-Mie Lee  Sangkyu Lee  Jong-Sup Bae  Jun-Goo Jee  Min Young Lee 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):17-23

Butylated hydroxyanisole (BHA) is a synthetic phenolic compound consisting of a mixture of two isomeric organic compounds: 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole. We examined the effect of BHA against hydrogen peroxide (H₂O₂)-induced apoptosis in primary cultured mouse hepatocytes. Cell viability was significantly decreased by H₂O₂ in a dose-dependent manner. Additionally, H₂O₂ treatment increased Bax, decreased Bcl-2, and promoted PARP-1 cleavage in a dose-dependent manner. Pretreatment with BHA before exposure to H₂O₂ significantly attenuated the H₂O₂-induced decrease of cell viability. H₂O₂ exposure resulted in an increase of intracellular reactive oxygen species (ROS) generation that was significantly inhibited by pretreatment with BHA or N-acetyl-cysteine (NAC, an ROS scavenger). H₂O₂-induced decrease of cell viability was also attenuated by pretreatment with BHA and NAC. Furthermore, H₂O₂-induced increase of Bax, decrease of Bcl-2, and PARP-1 cleavage was also inhibited by BHA. Taken together, results of this investigation demonstrated that BHA protects primary cultured mouse hepatocytes against H₂O₂-induced apoptosis by inhibiting ROS generation.  相似文献   

First discovery of a cave-dwelling Tineid moth （Lepidoptera,Tineidae） from East Asia     

Bong-Kyu Byun  ;Sat-Byul Shin  ;Yang-Seop Bae  ;Do Sung Kim  ;Yong Geun Choi 《林业研究》2014,25(3):647-651

In this study, we report Monopis crocicapitella （Clemens, 1859） （Tineidae）, which was collected from bat guano in a cave in the southern region of Korea, for the first time from East Asia. We briefly redescribe and illustrate the external morphology and genital structures of both sexes. Also, we discuss the local habitat characteristics and some of the ecological information that was observed during our field investi-gation.  相似文献