Ascorbic acid level of Belgian apple genetic resources     

V. Planchon  M. Lateur  P. Dupont  G. Lognay   《Scientia Horticulturae》2004,100(1-4):51-61

With the help of the public, a wide collection of old apple cultivars (cvs) was established progressively at our Department from 1975 onwards. Currently, more than 1400 apple accessions are being maintained and three quarters of them are subjected to field evaluation for disease resistance and agronomic characters. This paper presents results of the ascorbic acid (Vitamin C) contents of old cvs combining interesting disease resistance and agronomic features in order to promote them as cvs adapted to production systems which are more environmentally friendly or to be used as parents in breeding programmes. First, a methodological study shows the importance of the variability of ascorbic acid content depending on fruit position in the trees, fruit size, fruit skin colour and maturity stage. Statistical analyses show that the sample size of six fruits per cv is sufficient to detect a difference of 20% between cvs. Old apple cvs such as Grenadier^RGF, Transparente de Croncels, Bramley’s Seedling, Reinette des Capucins, Reinette Libotte and Reinette de Waleffe contain three and seven times more ascorbic acid than the commercial cvs Gala and Elstar, respectively. The paper highlights the need to evaluate the rich potential in our genetic resources.  相似文献   

Consequences of ignoring variable and spatially autocorrelated detection probability in spatial capture-recapture     

Moqanaki  Ehsan M.  Milleret  Cyril  Tourani  Mahdieh  Dupont  Pierre  Bischof  Richard 《Landscape Ecology》2021,36(10):2879-2895

Landscape Ecology - Spatial capture-recapture (SCR) models are increasingly popular for analyzing wildlife monitoring data. SCR can account for spatial heterogeneity in detection that arises from...  相似文献   

The Impact of Egg Ozonation on Hatching Success,Larval Growth,and Survival of Atlantic Cod,Atlantic Salmon,and Rainbow Trout     

Jessica Fry  Juan Pérez Casanova  Lynn Lush  Andy Walsh  Cyr Couturier 《Journal of aquatic animal health》2015,27(1):57-64

The direct exposure of fish eggs to ozonated water has generated interest as a means of ensuring pathogen-free eggs without the use of harsh chemicals. However, there are numerous knowledge gaps, including safe contact times, exposure levels, and potential long-term effects on aquaculture species in both freshwater and seawater. The effect of different ozone (O₃) doses (0.5–1.0, 1.5–2.0, and 2.5–3.0 mg of O₃/L for 90 s) on recently fertilized eggs of Atlantic Cod Gadus morhua and eyed eggs of Atlantic Salmon Salmo salar and Rainbow Trout Oncorhynchus mykiss was evaluated in comparison with the effects of two commercial disinfectants: Perosan (0.004 mg/L) and Ovadine (100 mg/L). The impact of ozone application was evaluated based on hatching success, larval nucleic acid concentration, larval growth, and survival. Overall, results indicated that ozonation of Atlantic Cod eggs at a dose less than 3.0 mg/L for 90 s produced no negative effect on the larvae up to 30 d posthatch. Furthermore, ozonation of Atlantic Salmon and Rainbow Trout eggs generated no negative effect on the larvae, based on monitoring until 85% yolk sac re-absorption (16 d posthatch).

Received May 6, 2014; accepted October 24, 2014  相似文献   

ELISA to detect proteolysis of ultrahigh-temperature milk upon storage     

Dupont D  Lugand D  Rolet-Repecaud O  Degelaen J 《Journal of agricultural and food chemistry》2007,55(17):6857-6862

Casein proteolysis can occur in milk during storage leading to its gelation. The two main proteolytic systems suspected to be involved are the plasmin and the proteases produced by psychrotrophic bacteria. The latter have been shown to cleave kappa-casein at the Phe105-Met106 bond. Although several techniques allow the determination of plasmin in milk, few rapid and easy-to-perform analytical techniques are available to check for bacterial proteolytic activity. This study presents the development of an inhibition ELISA allowing for the quantification of the kappa-casein intact at the Phe105-Met106 bond. It uses a monoclonal antibody specifically directed against this peptide bond that binds to the protein as long as the molecule's cleavage site is intact but not when it is cleaved. This simple technique allows for the rapid analysis of more than 20 samples within 3 h. Applied to commercial milks, this assay allowed for the detection of unstable milk.  相似文献   

Short-term survival and mortality rates in a retrospective study of colic in 1588 Danish horses     

Mogens T Christophersen  Nana Dupont  Kristina S Berg-S?rensen  Christel Konnerup  Tina H Pihl  Pia H Andersen 《Acta veterinaria Scandinavica》2014,56(1):20

Background

Outcomes of colic treatment are of great interest to clinicians, horse owners and insurers. One commonly used criterion of success is the overall short-term survival rate. This is used as to compare treatments and to measure quality of veterinary care, but may be biased by demographic or social factors such as attitudes towards animal suffering and euthanasia. The aims of this study were to 1) describe and analyse characteristics in horses with signs of colic referred to the University Hospital for Large Animals (UHLA), University of Copenhagen, Denmark over a 10-year period and 2) to compare these rates with those published in other comparable studies.

Results

The overall survival rate for colic horses over the 10-year study period was 68% (confidence intervals (CI): 66–71%; 1087/1588). In the medical group, 1093 horses, short-term survival was 87% (CI: 85–89%). Thirty one % of referred horses were given diagnoses requiring surgical intervention (CI: 29–33%). In this group 32% of the horses were euthanized before surgery (CI: 28–36%; 159/495). Of the surgical cases 27% (CI: 23-31%) were euthanized or died during surgery. Of the horses that recovered from surgery 25% died or were euthanized (CI: 19–32%; 48/189), while 75% survived to discharge (CI: 68–81%).

Conclusions

The short term survival rates of Danish horses with colic were similar or lower to those reported from other countries. Apart from variability of veterinary care, attitudes towards euthanasia vary among the countries, which may bias the outcomes. This study indicates that qualitative interview studies on owners’ attitudes towards animal suffering and euthanasia need to be conducted. Our opinion is that survival rates are not valid as sole indicators of quality of care in colic treatment due to selection bias. If the survival rates are to be compared between hospitals, techniques or surgeons, prospective studies including mutually agreed-on disease severity scores and a predefined set of reasons for euthanasia are needed.  相似文献   

Innate immune response to a H3N2 subtype swine influenza virus in newborn porcine trachea cells,alveolar macrophages,and precision-cut lung slices     

Mario Delgado-Ortega  Sandrine Melo  Darsaniya Punyadarsaniya  Christelle Ramé  Michel Olivier  Denis Soubieux  Daniel Marc  Ga?lle Simon  Georg Herrler  Mustapha Berri  Jo?lle Dupont  Fran?ois Meurens 《Veterinary research》2014,45(1):42

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Using operating area size and adjacency constraints to mitigate the effects of harvesting activities on boreal caribou habitat     

Guillaume Cyr  Frédéric Raulier  Daniel Fortin  David Pothier 《Landscape Ecology》2017,32(2):377-395

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Impacts of tree canopy structure on wind flows and fire propagation simulated with FIRETEC     

François Pimont  Jean-Luc Dupuy  Rodman R. Linn  Sylvain Dupont 《Annals of Forest Science》2011,68(3):523-530

Introduction   

Forest fuel management in the context of fire prevention generally induces heterogeneous spatial patterns of vegetation. However, the impact of the canopy structure on both wind flows and fire behavior is not well understood.  相似文献   

Antipeptide antibodies recognizing plasmin sensitive sites in bovine beta-casein sequence     

Senocq D  Dupont D  Rolet-Répécaud O  Faurie F  Levieux D 《Journal of agricultural and food chemistry》2001,49(3):1571-1577

To investigate plasmin activity in cheese, we produced antibodies to bovine beta-casein with controlled specificity, suitable as markers of the integrity of the major bonds involved in its initial breakdown. Sixteen rabbits were immunized with synthetic substitutes for six plasmin-sensitive peptides. Antisera raised to the peptides (f20-39), (f40-56), (f94-113), (f184-202), and (f193-209) recognized beta-casein in ACP-ELISA, Western-blot, and biosensor assays. Casein in vitro hydrolysis by plasmin or chymosin reduced the detection of these determinants in ACP-ELISA, in agreement with the enzymatic sensitivity of bonds included within the binding sites, or in their neighborhood. Antiserum to (f20-39) in particular allowed the specific detection of plasmin cleavage at the bond generating gamma1-CN. Antisera to C-terminus preferentially detected the cleavage by chymosin. Immunoassays using these antibodies would allow in situ monitoring of significant proteolysis events without bias originated in the secondary degradation of the released peptides.  相似文献   

Integration of TGF-beta and Ras/MAPK signaling through p53 phosphorylation     

Cordenonsi M  Montagner M  Adorno M  Zacchigna L  Martello G  Mamidi A  Soligo S  Dupont S  Piccolo S 《Science (New York, N.Y.)》2007,315(5813):840-843

During development and tissue homeostasis, cells must integrate different signals. We investigated how cell behavior is controlled by the combined activity of transforming growth factor-beta (TGF-beta) and receptor tyrosine kinase (RTK) signaling, whose integration mechanism is unknown. We find that RTK/Ras/MAPK (mitogen-activated protein kinase) activity induces p53 N-terminal phosphorylation, enabling the interaction of p53 with the TGF-beta-activated Smads. This mechanism confines mesoderm specification in Xenopus embryos and promotes TGF-beta cytostasis in human cells. These data indicate a mechanism to allow extracellular cues to specify the TGF-beta gene-expression program.  相似文献