全文获取类型
收费全文 | 18136篇 |
免费 | 189篇 |
国内免费 | 11篇 |
专业分类
林业 | 3700篇 |
农学 | 1364篇 |
基础科学 | 147篇 |
3113篇 | |
综合类 | 846篇 |
农作物 | 2160篇 |
水产渔业 | 2126篇 |
畜牧兽医 | 1799篇 |
园艺 | 1136篇 |
植物保护 | 1945篇 |
出版年
2023年 | 17篇 |
2022年 | 13篇 |
2021年 | 18篇 |
2020年 | 29篇 |
2019年 | 22篇 |
2018年 | 2813篇 |
2017年 | 2774篇 |
2016年 | 1250篇 |
2015年 | 117篇 |
2014年 | 83篇 |
2013年 | 203篇 |
2012年 | 888篇 |
2011年 | 2234篇 |
2010年 | 2159篇 |
2009年 | 1288篇 |
2008年 | 1389篇 |
2007年 | 1658篇 |
2006年 | 107篇 |
2005年 | 183篇 |
2004年 | 189篇 |
2003年 | 232篇 |
2002年 | 150篇 |
2001年 | 71篇 |
2000年 | 102篇 |
1999年 | 30篇 |
1998年 | 14篇 |
1997年 | 16篇 |
1996年 | 19篇 |
1995年 | 26篇 |
1994年 | 17篇 |
1993年 | 31篇 |
1992年 | 20篇 |
1991年 | 9篇 |
1990年 | 8篇 |
1989年 | 13篇 |
1988年 | 16篇 |
1986年 | 4篇 |
1985年 | 7篇 |
1984年 | 9篇 |
1983年 | 11篇 |
1982年 | 5篇 |
1980年 | 9篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 12篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1969年 | 5篇 |
1968年 | 4篇 |
1930年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
E. Conde A. M. Moreno I. Martin-Lacave A. Fernandez y H. Galera 《Anatomia, histologia, embryologia》1992,21(1):94-100
A systematic immunohistochemical study of the ultimobranchial tubule (UBT) has been carried out in 45 Wistar rats of different ages (0, 5, 10, 15, 20, 25, 30, 60 and 120 days). The existence of calcitonin immunoreactive cells in the UBT wall has been demonstrated in a 5-days old rat. In addition, immunohistochemical studies for thyroglobulin revealed positive staining in follicular cells connected to the UBT and, occasionally, in isolated cells lying within solid clusters from the UBT. These last results together with the continued and repeated existence of numerous mitosis and PAS (+) microfollicles, apparently rising from the UBT, support the hypothesis that the ultimobranchial body (UBB) may contribute partially to the formation of a part of the follicular component. 相似文献
4.
5.
Jørgen Lauritzen Jensen 《Journal of Wood Science》2005,51(6):577-582
A quasi-non-linear fracture mechanics model based on beam on elastic foundation theory is applied for analysis of the splitting
failure of dowel joints loaded perpendicular to grain. Simply supported beams symmetrically loaded by two dowels are considered,
and the effects of edge distance, dowel spacing, and distance between dowels and supports are accounted for. The foundation
modulus used in the beam on elastic foundation model is chosen so that the perpendicular-to-grain tensile strength and fracture
energy properties of the wood are correctly represented. This ensures that a conventional stress analysis and failure criterion
lead to the same solution as the compliance method of fracture mechanics. A semiempirical efficiency factor is proposed to
account for the influence of the total beam depth, which does not enter the beam on elastic foundation model, but the effect
of which is evident from tests. It is shown that the so-called Van der Put/Leijten model, which recently has been adopted
in Eurocode 5, appears as a special case of the model presented. Tests on simply supported beams with a single dowel joint
at midspan are compared with the theoretical predictions. Various edge distances, beam depths, and spans were tested. 相似文献
6.
7.
H. Kruse M. Hofshagen S. I. Thoresen W. P. Bredal I. Vollset N. E. Søli 《Veterinary research communications》1996,20(3):205-214
In a retrospective study, 1538 strains of -haemolysin-producing Staphylococcus species isolated from dermatitis in dogs at three veterinary clinical microbiology laboratories in Norway during 1986–87 and 1993–94 were investigated for their antimicrobial susceptibility. None of the strains was resistant to cloxacillin, cephalexin or the quinolones enrofloxacin and ciprofloxacin. More than 96% of the strains were susceptible to trimethoprim-sulphonamide, bacitracin and fucidic acid. Between 67% and 89% of the strains were susceptible to erythromycin, lincosamides, tetracycline, neomycin and chloramphenicol. Only 37.9% of the strains were susceptible to penicillin. The frequency of penicillin resistance increased significantly between the first and second periods, from 46.0% to 58.6%. The frequency of resistance to lincomycin, clindamycin and erythromycin also increased significantly between the first and second periods, from 3.0%, 2.1% and 3.3% to 25.5%, 19.5% and 24.8%, respectively. A moderate increase in resistance to tetracycline was also noted, from 20.4% in the first to 27.6% in the second period. On the other hand, the frequency of resistance to trimethoprim-sulphonamide decreased significantly from 4.1% in the first to 0.9% in the second period. Many different resistance patterns were observed in each period. However, the proportion of multiresistant strains increased from 2.1% in the first to 10.2% in the second period. There was a decrease in resistance to the combination of trimethoprim-sulphonamide and penicillin from the first to the second period. Resistance to the combination of lincosamides and pencillin increased. For the combinations penicillin-tetracycline-lincosamides, pencillin-lincosamides-erythromycin, and pencillin-tetracycline-lincosamides-erythromycin, there was a striking increase in resistance between the first and the second periods.Abbreviations CVL
Central Veterinary Laboratory
- NCVM
Norwegian College of Veterinary Medicine
- NVL
Norwegian Veterinary Laboratory 相似文献
8.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
9.
Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献
10.
Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献