Validation of a human immunoturbidimetric assay to measure canine albumin in urine and cerebrospinal fluid.     

Fabio Gentilini  Francesco Dondi  Cinzia Mastrorilli  Massimo Giunti  Claudia Calzolari  Gualtiero Gandini  Danilo Mancini  Paolo Famigli Bergamini 《Journal of veterinary diagnostic investigation》2005,17(2):179-183

The aim of this study was to validate an automated immunoturbidimetric assay used to quantify human albumin in urine and to accurately measure canine albumin concentrations in both urine and cerebrospinal fluid. The partial homology existing between human and canine albumin limited the accuracy of the human assays in measuring canine albumin without method modifications. Thus, the assay was modified by calibrating the analyzer with calibrators made in the laboratory containing known concentrations of canine albumin. To prepare the set of calibrators, the albumin concentration of pooled sera of healthy dogs was assessed in 5 replicates using the BromocresolGreen assay. Pooled samples were aliquoted and serially diluted to obtain the expected concentrations of albumin (0.5, 1, 5, 13, and 30 mg/dl) for establishing the canine calibration curve. Thereafter, the performance was assessed by analyzing canine urine and CSF The modified assay accurately quantified canine albumin in both specimens, as indicated by the following. Intra- and interassay variability was 0.92% and 2.74%, respectively; recovery was 99.66% and 99.07% in urine and 105.02% in CSF No interference was detected when hemolysate and glucose were added to urine. The test was linear within the verified range (0-225 mg/dl). These results demonstrate that the modified human albumin immunoturbidimetric assay can be a useful tool in the veterinary diagnostic laboratory. It is accurate and tends itself to automatization on chemistry analyzers.  相似文献   

Sedative and cardiorespiratory effects of low doses of xylazine with and without acepromazine in Nordestino donkeys          下载免费PDF全文

J. A. Lucas Castillo  M. Gozalo‐Marcilla  M. Werneck Fonseca  F. S. Possebon  A. C. da Rosa  A. J. de Araujo Aguiar 《Equine veterinary journal》2018,50(6):831-835

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Use of two doses of cloprostenol in different intervals for estrus synchronization in hair sheep under tropical conditions     

de Carvalho Menezes de Almeida  Sheylla Foligno  Souza-Fabjan  Joanna Maria Gonçalves  Balaro  Mario Felipe Alvarez  Bragança  Gláucia Mota  Pinto  Pedro Henrique Nicolau  de Almeida  José Gabriel  Moura  Ana Beatriz Bossois  da Fonseca  Jeferson Ferreira  Brandão  Felipe Zandonadi 《Tropical animal health and production》2018,50(2):427-432

Tropical Animal Health and Production - This study evaluated the effect of two doses of prostaglandin at different intervals on reproductive parameters of crossbred ewes. In Experiment 1, 30 ewes...  相似文献   

Feeding behavior of feedlot lambs fed diets containing levels of cassava wastewater     

Paula de Aguiar Silva  Gleidson Giordano Pinto de Carvalho  Aureliano José Vieira Pires  Stefanie Alvarenga Santos  Douglas dos Santos Pina  Robério Rodrigues Silva  Carlindo Santos Rodrigues  Luis Henrique Almeida de Matos  Carlos Emanuel Eiras  Daiane Novais-Eiras  Wilson Souza Nunes 《Tropical animal health and production》2018,50(4):721-726

In this study, we evaluated the effects of including cassava wastewater in the diet on the feeding behavior of feedlot lambs in 35 male uncastrated Santa Inês × Dorper crossbred lambs at an approximate age of 3 months, with an average live weight of 20.0?±?3.4 kg. Diets were formulated with hay of cassava shoots (roughage) and a concentrate based on corn and soybean, with a roughage:concentrate ratio of 50:50, plus inclusion of cassava wastewater at the levels of 0, 12, 24, 36, or 48 g/kg of the total diet. Feeding behavior was evaluated between the 46th and 52nd days of the experiment. Increasing cassava wastewater levels in the diet reduced (P?<?0.05) the intakes (kg/day) of dry matter and neutral detergent fiber as well as the efficiency of rumination (g/cud and g/h) of dry matter and neutral detergent fiber. The other behavioral parameters were not affected by wastewater inclusion in the diet. Therefore, the inclusion of up to 48 g/kg of cassava wastewater on fresh matter of diets is not recommended for feedlot lambs.  相似文献   

Aboveground biomass,transpiration and water use efficiency in eucalypt plantation fertilized with KCl,NaCl and phonolite rock powder     

de Toledo  Fábio Henrique S. F.  de Moraes Gonçalves  José Leonardo  Mariño  Yesid Alejandro  de Vicente Ferraz  Alexandre  de Oliveira Ferreira  Eric Victor  Moreira  Gabriela Gonçalves  Hakamada  Rodrigo  de Arthur Júnior  José Carlos 《New Forests》2020,51(3):469-488

Potassium has important physiological functions in eucalypt plantations, increasing their productivity when applied to soil via mineral fertilizers. There is interest in identifying alternative sources to KCl owing to its high cost and limited reserves. The aim of the study was to test the effect of replacing KCl with NaCl and phonolite rock powder. Two comparisons were made: (1) application of 283 kg ha^?1 of KCl compared with that of 2125 kg ha^?1 of phonolite rock powder (equivalent to 170 kg ha^?1 of K₂O in both treatments); (2) application of 139 kg ha^?1 of NaCl compared with that of 183 kg ha^?1 of KCl (equivalent to 2.33 kmol Na and K, respectively). Radial growth, soil water content, leaf water potential (Ψ), accumulated transpiration, stem volume and biomass increment, as well as water use efficiency (WUE) were evaluated. In the first comparison, both fertilizations presented equal values for all characteristics evaluated. In the second, the accumulated transpiration in trees fertilized with KCl was 17% higher than that in plants fertilized with NaCl. In contrast, the WUE was 20% higher in the trees fertilized with NaCl than in those fertilized with KCl, reflecting the lower water consumption for the same increment in stem volume and biomass. We conclude that phonolite rock powder and NaCl are possible substitutes for conventional K fertilization performed with KCl.

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A new standard area diagram set for assessment of severity of soybean rust improves accuracy of estimates and optimizes resource use     

Vinicius T. Franceschi  Kaique S. Alves  Sergio M. Mazaro  Cláudia V. Godoy  Henrique S. S. Duarte  Emerson M. Del Ponte 《Plant pathology》2020,69(3):495-505

Soybean rust (SBR), caused by Phakopsora pachyrhizi, is the most important yield-damaging fungal disease of soybean due to severe reduction in healthy leaf area and acceleration of leaf fall. In experimental research, SBR severity is estimated visually aided/trained by a standard area diagram (SAD) developed and validated during the mid-2000s (Old SAD). In this study, we propose a new SAD set for SBR with six true-colour diagrams following linear increments (c. 15% increments) amended with four additional diagrams at low (<10%) severities, totalling 10 diagrams (0.2%, 1%, 3%, 5%, 10%, 25%, 40%, 55%, 70%, and 84%). For evaluation, 37 raters were split into two groups. Each assessed severity in a 50-image sample (0.25%–84%), first unaided and then using either the Old SAD or the New SAD. Accuracy, precision, and reliability of estimates improved significantly relative to unaided estimates only when aided by the New SAD (accuracy >0.95). Low precision (<0.78) and a trend of underestimation with an increase in severity were the main issues with the Old SAD, which did not differ from unaided estimates. Simulation to evaluate the impact of the errors by different methods on hypothesis tests, showed that the new SAD was more powerful for detecting the smallest difference in mean control (e.g., 70% vs. 65% disease reduction) than the Old SAD; the latter required a 2-fold increase in sample size to achieve the same power. There is a need to improve some SADs, taking advantage of new knowledge and technology to increase accuracy of the estimates, and to optimize both resource use efficiency and management decisions.  相似文献   

Dynamics and efficacy of sulfentrazone,flumioxazin, and isoxaflutole herbicides applied on eucalyptus harvest residues     

Carbonari  Caio Antonio  Gomes  Giovanna Larissa Gimenes Cotrick  Krenchinski  Fábio Henrique  Simões  Plinio Saulo  Batista de Castro  Edicarlos  Velini  Edivaldo Domingues 《New Forests》2020,51(4):723-737

New Forests - Eucalyptus harvests generate residues that remain on the soil and affect the efficacy of preemergent herbicides in the following planting cycle. The objective of this work was to...  相似文献   

Jack beans and vetiver grass growth on iron ore tailing sediments from the Doce River dam disaster in Brazil: plant growth regulator effects under different edaphic conditions     

Santana  Pedro Henrique Lopes  Burak  Diego Lang  Thiengo  Cássio Carlette  Peçanha  Anderson Lopes  Neves  Mirna Aparecida  Mendonça  Eduardo de Sá 《Journal of Soils and Sediments》2020,20(11):4103-4110

Journal of Soils and Sediments - After the greatest environmental disaster in the history of Brazil and the deposition of the iron ore tailings in alluvial regions, the process of revegetation for...  相似文献   

Soil physical changes and maize growth in a structurally fragile tropical soil due to mulching and duration between irrigation intervals          下载免费PDF全文

E. G. Moura  V. R. A. Macedo  V. G. L. Sena  L. S. Campos  A. C. F. Aguiar 《Soil Use and Management》2017,33(4):631-638

Under tropical meteorological conditions, the volume of soil explored by plant roots is crucial for crop growth as it allows increased water and nutrient use efficiency. We hypothesized that, under different irrigation intervals, leguminous mulch can extend the duration between irrigation events but maintain crop performance, because decreased evaporative fluxes also reduce constraints to root exploration imposed by mechanical stress. We evaluated the combined effects of leguminous mulch and irrigation intervals on soil physical properties to determine whether the growth and productivity of maize were modified in a structurally fragile tropical soil. The experiment involved the following treatments: 4‐day irrigation intervals with soil mulched (4C) or bare (4S), 6‐day irrigation intervals with soil mulched (6C) or bare (6S), 8‐day irrigation intervals with soil mulched (8C) or bare (8S) and 10‐day irrigation intervals with soil mulched (10C) or bare (10S). Mulch decreased soil penetration resistance and increased to 4 days the favourable time for root development in drying soil. Relative to bare soil, mulch with a 6‐day irrigation interval almost doubled nitrogen uptake post‐tasselling, which decreased nitrogen remobilization and increased the crop growth rate during this stage. These conditions had a positive effect on the transpiration rate and stomatal conductance as well as on the growth and yield of maize. A 6‐day irrigation interval with mulch compared to 4 days with bare soil resulted in similar conditions for root development, but greater uptake of nitrogen (102.73–78.70 kg/ha) and better yield (6.2–5.3 t/ha), which means greater efficiency in nitrogen and water use.  相似文献   

Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean     

Linnajara DE VASCONCELOS MARTINS FERREIRA  Fernanda DE CARVALHO  J&#;lia FONSECA COLOMBO ANDRADE  Damiany P&#;DUA OLIVEIRA  Fl&#;vio Henrique VASCONCELOS DE MEDEIROS  Fatima Maria DE SOUZA MOREIRA 《土壤圈》2020,30(1):98-108

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia solani.However,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase production.In this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield levels.The aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean plants.Greenhouse experiments were conducted under axenic conditions using the common bean cultivar Pérola.The first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of R.solani.The second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common bean.Co-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping off.For the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/02-293.Nodule endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial inoculation and protection against disease.  相似文献