排序方式： 共有15条查询结果，搜索用时 15 毫秒
1.
Shaishav Sharma Ekta Shah Deepti Davla Gaurav Dixit Aesha Patel Adepu Kiran Kumar 《International Aquatic Research》2019,11(4):377-387
Here an indigenously isolated microalgal strain Ascochloris spp. cultivated in synthetic medium was evaluated as an aquaculture feed supplement. The daily dietary supplement includes microalgal feed (AF) and commercial diet feed (CF) (as control), respectively. These diets were fed separately to the juvenile Clarias gariepinus fishes (n = 4) under controlled conditions for an experimental period of 100 days. The protein, glycogen and lipid contents in the muscle extracts were found to be marginally higher in fishes that were fed with CF than AF diet. Similarly, CF fishes showed significantly higher glutathione-s-transferase, catalase, superoxide dismutase, and lipid peroxidase activities, except glutathione content. Zero mortality of the fishes with no significant difference in the overall body mass with the two dietary supplements strongly suggests that algal biomass could supplement the requisite nutrients for their metabolic activities. This preliminary investigation helps in exploring algal biomass as a potential alternative feed additive in the aquaculture industry. 相似文献
2.
Singh Deepti Sharma Krishna Kant Jacob Shenu Gakhar S. K. 《Water, air, and soil pollution》2014,225(11):1-12
Water, Air, &; Soil Pollution - The bacterial communities in the intestinal tracts of earthworm were isolated by culture-dependent approaches. In total, 72 cultures were isolated and purified... 相似文献
3.
Sarika Diwaniyan Deepti Kharb Chandralata Raghukumar Ramesh Chander Kuhad 《Water, air, and soil pollution》2010,210(1-4):409-419
Decolorization of six synthetic dyes and two raw textile effluents (A and B) by eight basidiomycetous fungi was investigated. Among eight basidiomycetous fungi, fungal isolate RCK-1 decolorized textile effluent A maximally (42%), while fungal isolate RCK-3 was found to decolorize more of Congo Red (69%), Xylidine Ponceau 2R (100%), Poly R-478 (96%), Indigo Carmine (99%), Lissamine Green B (90%), Toluidine Blue (57%) and textile effluent B (54%), than the rest of fungi. Percentage decolorization of all synthetic dyes and textile effluents by the new fungal isolates RCK-1 and RCK-3 was higher compared to the most widely studied simultaneous lignin degrader, Phanerochaete chrysosporium and selective lignin degrader, Pycnoporus cinnabarinus, when tested in liquid cultures. A statistically significant positive correlation between laccase production and decolorization of dyes and effluents was obtained as compared to other ligninolytic enzymes (lignin peroxidase and manganese peroxidase) production. This showed the importance of the differential contribution of the different ligninolytic enzymes towards the decolorization of the synthetic dyes and textile effluents. The substantially higher ligninolytic enzyme production by the fungal isolates RCK-1 and RCK-3 also suggested their potential use for textile effluent treatment and other possible biotechnological applications. 相似文献
4.
5.
Season and concentration of sterilizing agents play a significant role for establishment of aseptic in vitro shoot cultures and sprouting of nodal explants from field growing culms of bamboo species. In the present investigation the nodal segment explants of Bambusa tulda Roxb collected in different seasons and treated with various concentrations of HgCl2 showed significant variation in aseptic culture establishment and bud break. The rainy season (July–August) recorded with highest of 78% aseptic culture establishment whereas autumn recorded with lowest 46%. Summer and winter seasons emerged to be the best period, registering > 60% in vitro bud break. On the other hand, the autumn season had the lowest value for bud break, i.e. 42%. Among different doses of sterilizing agent tried, HgCl2 0.1% found to be suitable for maximum aseptic culture establishment (66%) as well as bud break (59%). However, among the interactions, summer season and the dose of 0.1% HgCl2 exhibited maximum of 73% response for both aseptic culture establishment and bud break. MS medium (liquid) enriched with 5.0 µM BA + 5.0 µM Kn [Kinetin (N6-Furfuryladenine)] with additional supplementation of 100 µM glutamine + 0.1 µM IAA supported a maximum in vitro shoot multiplication of 4.75 fold. The proliferated shoots were successfully rooted on MS medium (liquid) supplemented 40 µM coumarin. The plantlets transferred to the polythene bags showed 98% survival. 相似文献
6.
Tanu Deepti D Deobagkar Rakhee Khandeparker Rayadurga A Sreepada Sushant V Sanaye Hrishikesh B Pawar 《Aquaculture Research》2012,43(3):386-394
The microenvironment of bacteria associated with the gastrointestinal tract of an animal influences the host in many ways, including the metabolism of several nutrients. Isolation, molecular characterization and enzymatic activities of culturable bacteria associated with the gastrointestinal tract of hatchery‐reared oceanic yellow seahorse, Hippocampus kuda, were investigated in the present study. 16S rRNA sequencing of bacterial isolates yielded 10 different bacterial genera belonging to two major groups: proteobacteria (62.9%) and firmicutes (37.03%). The predominant microbial flora belonged to the genera Vibrio spp., Enterovibrio sp. and Bacillus spp. Burkholderia cenocepacia, hitherto unreported from the gastrointestinal tract of marine fish, was retrieved from the seahorse gut, albeit in small numbers. Enzymatic studies indicated that the bacterial isolates possess the ability to degrade lipids, cellulose, xylan, starch and proteins. Lipase activity was exhibited by a majority of the isolates (70.4%), indicating that the bacterial flora associated with the intestinal tract of seahorses is capable of digesting foods rich in lipids. The possible implications for the role of these bacteria in the digestion and physiological processes are discussed. To our knowledge, this is the first time that the characterization and enzymatic activities of bacteria associated with the intestinal tract of farmed seahorses have been reported. 相似文献
7.
Rice, one of the most important staple food crops of the world, suffers a major setback nutritionally, because it is deficient in bioavailable zinc. In an attempt to answer this problem a field study was performed for two years during 2010 and 2011. Rice plants were treated with selected plant growth promoting rhizobacteria (PGPR; P. putida, P. fluorescens, A. lipoferum, B 15, B 17, B 19, BN 17, and BN 30) and plant growth, zinc (Zn) content in different plant parts and grains was analyzed. The data obtained showed enhancement in rice growth and hence, increased rice yield in response to PGPR application. All isolates resulted in almost 1.5- to 2-fold increase in Zn content in roots, shoots as well as grains in comparison to the control. The bacterial isolates B 17, B 19, and BN 17 were of particular interest as they induced the movement of Zn from roots to shoots as well as from husk to the grains, thus making grains enriched in Zn (around 25% higher Zn content). Therefore, it can be concluded that application of PGPR strains is an important strategy to combat the problem of zinc deficiency in rice and consecutively in human masses. 相似文献
8.
CYP19 (cytochrome P450 aromatase) gene polymorphism in murrah buffalo heifers of different fertility performance 总被引：1，自引：0，他引：1
O. Suneel Kumar Deepti Sharma Dheer Singh M.K. Sharma 《Research in veterinary science》2009,86(3):427-437
The most common cause of infertility in buffaloes is anestrum. During late maturity the ovaries are in a state of true anestrum. One of the predominant causes of true anestrum is a low level of ovarian estrogens. The key enzyme in estrogen biosynthesis is cytochrome P450 aromatase, encoded by CYP19 gene. In the present study, CYP19 gene polymorphism was analyzed by Single Strand Conformational Polymorphism (SSCP) in buffaloes of different fertility performance. The SSCP and sequence analysis revealed 4 allelic variants in coding exons and introns which unaltered the protein sequence. However, a significant polymorphism (T/C heterozygote) was found near TATA binding protein region in regulatory part (a facet of promoter II) at position 23 of CYP19 exon 2, in all late matured and 50% of late maturing animals. Based on these observations and remarks of earlier workers, a hypothesis is proposed for the physiology of late maturity in buffaloes. 相似文献
9.
Season and concentration of sterilizing agents play a significant role for establishment of aseptic in vitro shoot cultures and sprouting of nodal explants from field growing culms of bamboo species.In the present investigation the nodal segment explants of Bambusa tulda Roxb collected in different seasons and treated with various concentrations of HgCl_2 showed significant variation in aseptic culture establishment and bud break.The rainy season(July–August) recorded with highest of 78% aseptic culture establishment whereas autumn recorded with lowest 46%.Summer and winter seasons emerged to be the best period, registering60% in vitro bud break.On the other hand, the autumn season had the lowest value for bud break,i.e.42%.Among different doses of sterilizing agent tried,HgCl_2 0.1% found to be suitable for maximum aseptic culture establishment(66%) as well as bud break(59%).However, among the interactions, summer season and the dose of 0.1% HgCl_2 exhibited maximum of 73% response for both aseptic culture establishment and bud break.MS medium(liquid) enriched with 5.0 μM BA+5.0 μM Kn[Kinetin(N6-Furfuryladenine)] with additional supplementation of 100 μM glutamine+0.1 μM IAA supported a maximum in vitro shoot multiplication of 4.75 fold.The proliferated shoots were successfully rooted on MS medium(liquid) supplemented 40 μM coumarin.The plantlets transferred to the polythene bags showed 98% survival. 相似文献
10.
Atmospheric nitrogen fixation as a result of the symbiosis between bacteria and legume species, can result in major advantages in providing host plants with organic nitrogen. The objective of the present study was to evaluate the physiological potential during early seedling development for initiation of nodulation and nitrogen fixation activity of four grain legumes species: soybean [Glycine max (L.)], cowpea [Vigna unguiculate (L.) Walp], common bean [Phaseolus vulgaris (L.)], and peanut [Arachis hypogaea (L.)]. Seedlings were grown on a hydroponic solution so that nodule development could be readily observed until about 3 weeks after germination. Nodules developed in all cases. Acetylene reduction activity (ARA) by soybean and cowpea was also found early in seedling development. In contrast, peanut and common bean showed little or no development of ARA during seedling development. The results provided insight into differences in physiological potential among grain legumes in establishing symbiotic nitrogen fixation during crop establishment. These results indicate those species/cultivars that are candidates for readily establishing nitrogen fixation activity during the seedling stage of plant development. 相似文献