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1.
Cunha MV Matos F Canto A Albuquerque T Alberto JR Aranha JM Vieira-Pinto M Botelho A 《Research in veterinary science》2012,92(2):225-235
Mycobacterium bovis and, more rarely, Mycobacterium caprae, may cause zoonotic bovine tuberculosis (bTB) in an extensive range of animal species. In Portugal, during 2009, a remarkable raise of bTB incidence was registered in cattle along with an increase of new cases in wildlife. In this work, we reassess and update the molecular epidemiology of bTB in wild ungulates by including 83 new M. bovis and M. caprae isolates from wild boar and red deer obtained during 2008-2009. Spoligotyping identified 27 patterns in wild ungulates, including 11 patterns exclusive from deer and five from wild boar. The genetic relatedness of wildlife and livestock isolates is confirmed. However, the relative prevalence of the predominant genotypes is different between the two groups. Contrasting with the disease in livestock, which is widespread in the territory, the isolation of bTB in wildlife is, apparently, geographically localized and genotypic similarities of strains are observed at the Iberian level. 相似文献
2.
Marta Monteiro Carla Sousa Filipe Coutinho Carolina Castro Filipa Fontinha Inês Guerreiro Pedro Pouso Elisabete Matos Patrícia Díaz-Rosales Aires Oliva-Teles Paula Enes Ana Couto 《Marine drugs》2021,19(11)
Marine algae are recognised sources of bioactive compounds that have attracted great interest as nutritional supplements for aquaculture fish. Intensive rearing conditions often expose fish to husbandry-related stressors, rendering fish more susceptible to disease and reducing production yields. The present work evaluated the potential of two marine algae extracts (Fucus vesiculosus and Nannochloropsis gaditana) as nutritional supplements to mitigate stress effects in meagre (Argyrosomus regius) exposed to an acute handling stress (AS). A plant-based diet was used as a control, and three other diets were prepared, which were similar to the control diet but supplemented with 1% of each algal extract or a combination of the two extracts (0.5% each). The effects of supplemented diets on stress biomarkers, antioxidant enzyme activities, and immune response were analysed in fish exposed to AS after 4 weeks of feeding. Supplemented diets did not affect growth performance but the inclusion of F. vesiculosus promoted higher feed efficiency, as compared to the control group. Dietary algal extracts supplementation reduced plasma glucose levels, increased white blood cell counts, and reduced the expression of pro-inflammatory genes when compared with the control. N. gaditana supplementation led to a reduction in hepatic antioxidant enzyme activity and glutathione levels, while F. vesiculosus supplementation increased muscle glutathione reductase activity and reduced lipid peroxidation. These findings support the potential of algal extracts as nutraceuticals in aquafeeds to enhance the ability of fish to cope with husbandry-related stressful conditions and ultimately improve fish health and welfare. 相似文献
3.
É.G. Castanheira 《Agricultural Systems》2010,103(7):498-507
The activities associated with raw milk production on dairy farms require an effective evaluation of their environmental impact. The present study evaluates the global environmental impacts associated with milk production on dairy farms in Portugal and identifies the processes that have the greatest environmental impact by using life cycle assessment (LCA) methodology. The main factors involved in milk production were included, namely: the dairy farm, maize silage, ryegrass silage, straw, concentrates, diesel and electricity. The results suggest that the major source of air and water emissions in the life cycle of milk is the production of concentrates. The activities carried out on dairy farms were the major source of nitrous oxides (from fuel combustion), ammonia, and methane (from manure management and enteric fermentation). Nevertheless, dairy farm activities, which include manure management, enteric fermentation and diesel consumption, make the greatest contributions to the categories of impact considered, with the exception of the abiotic depletion category, contributing to over 70% of the total global warming potential (1021.3 kg CO2 eq. per tonne of milk), 84% of the total photochemical oxidation potential (0.2 kg C2H4 eq. per tonne of milk), 70% of the total acidification potential (20.4 kg SO2 eq. per tonne of milk), and 41% of the total eutrophication potential (7.1 kg eq. per tonne of milk). The production of concentrates and maize silage are the major contributors to the abiotic depletion category, accounting for 35% and 28%, respectively, of the overall abiotic depletion potential (1.4 Sb eq. per tonne of milk). Based on this LCA case study, we recommend further work to evaluate some possible opportunities to improve the environmental performance of Portuguese milk production, namely: (i) implementing integrated solutions for manure recovery/treatment (e.g. anaerobic digestion) before its application to the soil as organic fertiliser during maize and ryegrass production; (ii) improving manure nutrient use efficiency in order to decrease the importation of nutrients; (iii) diversifying feeding crops, as the dependence on two annual forage crops is expected to lead to excessive soil mobilisation (and related impacts) and to insignificant carbon dioxide sequestration from the atmosphere; and (iv) changing the concentrate mixtures. 相似文献
4.
Rodrigo Otávio
De Almeida Ozório Carlos Andrade Viriato Manuel Freitas Andrade Timóteo Luís Eugénio Castanheira da Conceição Luisa Maria Pinheiro Valente 《Journal of the World Aquaculture Society》2009,40(1):95-103
Although reported as a slow growing species, to date information regarding nutritional dynamics of blackspot seabream, Pagellus bogaraveo, is limited, especially during ongrowing phase. This study determined the effects of different feeding levels on growth response, body composition, and energy expenditure of blackspot seabream juveniles (initial weight = 64 g). Fish were allocated in nine indoor tanks (15 fish per tank) and fed a basal diet twice daily at increasing levels (2.5, 3.3, and 4.1 g feed/kg0.8/d) for 138 d. Growth response decreased with decreasing feeding levels, but only at 2.5 g feed/kg0.8/d (P < 0.001). Differences in growth rates were positively correlated with body lipid content (r2 = 0.90). Net protein utilization decreased with each increase in feeding level. The energy requirement for zero growth (maintenance) and the estimated energy loss on starvation were calculated as 22.6 and 17.9 kJ/kg0.8/d, respectively. The energy cost for protein and lipid gain was calculated as 3.8 and 1.4 kJ/kJ energy deposited, respectively. This study suggested that blackspot seabream may grow slower than the other close‐related species because of its higher demand of dietary protein energy in favor of lipid deposition. 相似文献
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Catarina Campos M. Filipa Castanheira Sofia Engrola Luísa M. P. Valente Jorge M. O. Fernandes Luís E. C. Conceição 《Fish physiology and biochemistry》2013,39(6):1485-1496
The present work examined the short- and long-term effects of three rearing temperatures on protein metabolism and growth trajectories of Senegalese sole larvae using 14C-labelled Artemia protein as feed. A first feeding trial was performed on larvae reared at 15, 18 and 21 °C (at 26, 17 and 14 days post-hatching (dph), respectively) and a second trial conducted on post-larvae after transfer to the same rearing temperature (~20 °C) (49, 35 and 27 dph, in larvae initially reared at 15, 18 and 21 °C, respectively). Temperature greatly influenced larvae relative growth rate (RGR) and survival, since growth at 15 °C was severely depressed. Protein digestibility and retention was highest at 18 °C during the first trial (85.35 ± 1.16 and 86.34 ± 2.33 %, respectively). However, during the second trial, post-larvae from 15 °C had the highest feed intake and protein digestibility (3.58 ± 1.54 and 75.50 ± 1.35 %, respectively), although retention was similar between treatments. Furthermore, after transfer to 20 °C larvae from 15 °C experienced compensatory growth, which was observed until 121 dph, and confirmed by RGR values, which were significantly higher at 15 ºC than at 21 ºC or 18 ºC. Results from the present study show that Solea senegalensis larval development, survival and protein digestion and retention are highly affected by thermal history. 相似文献
8.
Marlos Castanheira Samuel Rezende Paiva Helder Louvandini Aline Landim Maria Clorinda Soares Fiorvanti Giane Regina Paludo Bruno Stefano Dallago Concepta McManus 《Tropical animal health and production》2010,42(2):185-191
The environment in which the horse is reared affects its ability to maintain thermal balance which is in turn related to thermal characteristics and regulatory physiological mechanisms. In this study a multivariate analysis of physiological traits in relation to heat tolerance in horses was carried out in the Federal District, Brazil. The aim was to test the ability of these analyses to separate groups of animals and determine which physiological traits are most important in the adaptation to heat stress. Forty adult horses (4 to 13 years) were used, ten from each of four different genetic groups (English thoroughbred, Brazilian showjumper, crossbred and Breton). The traits examined included heart and breathing rate, rectal temperature as well as blood parameters. The data underwent multivariate statistical analysis including cluster, discriminate and canonical using Statistical Analysis System - SAS ® procedures CLUSTER, STEPDISC, CANCORR and DISCRIM. The tree diagram showed clear distances between groups studied and canonical analysis was able to separate individuals in groups. The discriminate analysis identified the variables which were most important in separating these groups. The multivariate analysis was able to separate the animals into groups with RR, HR and RT being important in this separation. 相似文献
9.
Landim AV Cardoso MT Castanheira M Fioravanti MC Louvandini H McManus C 《Tropical animal health and production》2011,43(8):1561-1566
This study aims to investigate the effect of breed and slaughter weight on fatty the acid profile of the Longissimus dorsi muscle in lambs. Twenty-four Santa Inês (SI), 24.5 Ile de France × 0.5 Santa Inês (Ile × SI) and 12.5 Texel × 0.5 Santa Inês
(Te × SI), slaughtered at different weights (30, 35, 40, and 45 kg), were evaluated. The animals were reared with creep feeding
to weaning and were feedlot finished, receiving a diet composed of 30% hay and 70% concentrate. The analyses of fatty acids
were carried out on the Longissimus muscle of the 13th rib. The experiment was in a three by four factorial design. The total mean saturated fatty acid level
was 44.88%, with 43.30% monounsaturated fats and 1.72% polyunsaturated. The major fatty acids found included oleic (43%),
palmitic (22%), and stearic (18%). The concentration of desirable fatty acids varied from 61.56% to 66.78%, with Te × SI (66.78%)
having the highest levels (P < 0.05). The slaughter weight affected (P < 0.05) the saturated and unsaturated (both mono and poly) fatty acid profiles. The 35-kg slaughter weight showed the most
desirable fatty acid profile. The saturated myristic and stearic fatty acids decreased with an increase in the slaughter weight,
but oleic acid increased. In the conditions of the present study, the meat from Santa Ines and Texel × Santa Ines lambs had
fatty acid profiles more beneficial for human health due to the higher levels of unsaturated fatty acids. 相似文献
10.
Rauter AP Branco I Lopes RG Justino J Silva FV Noronha JP Cabrita EJ Brouard I Bermejo J 《Fitoterapia》2007,78(7-8):474-481
A new lupene triterpenetriol was isolated from the acetone extract of the aerial parts of Salvia sclareoides and characterised as (1beta,3beta)-lup-20(29)-ene-1,3,30-triol (1). In addition, nepetidin (2), nepeticin (3), lupendiol (4), (1beta,11alpha)-dihydroxy-lup-20(29)-en-3-one (5), ursolic acid (6), sumaresinolic acid (7) and hederagenin (8), were identified in this Salvia sp. To the best of our knowledge, the compounds 2 and 7 are new constituents in Salvia spp. The acetone, ethanol, butanol and water extracts of the plant were screened for the in vitro inhibitory activity of acetylcholinesterase (AChE) and butyrilcholinesterase (BChE), enzymes which play a role in the Alzheimer disease. All extracts inhibited acetylcholinesterase activity at 10 microg/ml, a remarkable activity since the standard drug rivastigmine does not inhibit acetylcholinesterase at the same concentration. Regarding the butyrilcholinesterase, the acetone extract at 1000 microg/ml was able to inhibit completely the enzyme activity and the butanol and ethanol extracts, at this concentration, produced a potent inhibition of BchE. 相似文献