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1.
Temperature demands of microscopic soil fungi from different climatic and geographical sites. . 154 strains of microscopic soil fungi were isolated at 25°C from soils deriving from tropical to alpine climatic zones of the world (SW-Africa, Argentine, Nepal, Canary Islands and W-Germany). Determining their saccharolytic, amylolytic and proteolytic activities on saccharose-, starch- or gelatine-containing agar plates at 5 °C, 15 °C, 25 °C, 35 °C and 43 °C, the activity range as well as the optimal temperature were obtained. The following results were obtained: 1) A clear correlation between the temperature requirements of the fungal flora and the climatic conditions of the site of isolation was observed. 2) The fungal flora of a certain climatic zone was composed of strains of ubiquitously distributed species. Their great adaptability enables them to accomodate themselves to the temperature conditions of each site. 3) A smaller percentage of species was characterised by a small range of temperature variability and therefore depending on a certain climatic zone. Some of these were strict pschychrophilic species, characteristic for alpine and subalpine regions, but others behaved as strict mesophiles observed only in warm regions. 4) Finally from each site of isolation there also could be isolated a certain amount of fungi, whose ecological demands did not or only for a very short time coincide with the bioclimatic conditions of the particular site. Generally, these isolates are able to form resistant spores enabling them to survive unfavourable climatic conditions. .  相似文献   
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Water balance and leaching of plant nutrients, with special reference to N, were described for a 46-ha catchment consisting mainly of coniferous forest (one third of it clear-cut) during the period January 1982-August 1988. The atmospheric N load in this region is high compared with most other parts of Scandinavia. On average, annual N leaching amounted to 9.5 kg ha?1 in the form of NO3-N (83%), org-N (15%) and NH4-N (2%). The highest monthly rate of N transport observed was 3.9 kg ha?1. The NO3-N levels in groundwater in the 60-yr-old coniferous stand ranged from 0.5 to 3.1 mg L?1. The effect of clear-cutting on groundwater-NO3-N levels lasted 4 yr. The highest annual NO3-N transport from the clear-cut area observed was 18 kg ha?1. The groundwater in the spruce forest was very acidic (pH=4.3) in contrast to the stream water (pH=6.3). The relatively higher pH-value of the stream water was probably a result of chemical and biological processes occurring in the highly humified, periodically waterlogged peat soil (alder swamp) in the vicinity of the small stream.  相似文献   
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A recently developed HPTLC/UV-FLD method was compared to the routinely used HPLC/UV-FLD method for the quantification of heterocyclic aromatic amines (HAA) formed at trace levels during the heating process of meat. For formation of these process contaminants under normal cooking conditions, beef patties were fried in a double-contact grill at 230 degrees C for five different frying times and extracted by solid-phase extraction. The HAAs most frequently found, that is, 2-amino-1-methyl-6-phenylimidazo[4,5- b]pyridine (PhIP), 2-amino-3,8-dimethylimidazo[4,5- f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5- f]quinoxaline (4,8-DiMeIQx), 9 H-pyrido[3,4- b]indole (norharman), and 1-methyl-9 H-pyrido[3,4- b]indole (harman), were quantified by two chromatographic methods, which were orthogonal to each other (normal versus reversed phase system). Both methods showed a similar performance and good correlation of the results ( R (2) between 0.8875 and 0.9751). The comparison of running costs and run time in routine analysis proved HPTLC/UV-FLD to be more economical (factor of 3) and faster (factor of 4) due to its capability of parallel chromatography. The HAA findings calculated by standard addition increased with the heating time from <1 to 33 microg/kg related to 3-6 min of frying time. The precision (RSD) was between 7 and 49% (HPTLC) and between 5 and 38% (HPLC) at these very low HAA levels formed.  相似文献   
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Historical data are widely used in river ecology to define reference conditions or to investigate the evolution of aquatic systems. Most studies rely on printed documents from the 19th century, thus missing pre‐industrial states and human impacts. This article discusses historical sources that can be used to reconstruct the development of riverine fish communities from the Late Middle Ages until the mid‐20th century. Based on the studies of the Austrian Danube and northern Russian rivers, we propose a classification scheme of printed and archival sources and describe their fish ecological contents. Five types of sources were identified using the origin of sources as the first criterion: (i) early scientific surveys, (ii) fishery sources, (iii) fish trading sources, (iv) fish consumption sources and (v) cultural representations of fish. Except for early scientific surveys, all these sources were produced within economic and administrative contexts. They did not aim to report about historical fish communities, but do contain information about commercial fish and their exploitation. All historical data need further analysis for a fish ecological interpretation. Three case studies from the investigated Austrian and Russian rivers demonstrate the use of different source types and underline the necessity for a combination of different sources and a methodology combining different disciplinary approaches. Using a large variety of historical sources to reconstruct the development of past fish ecological conditions can support future river management by going beyond the usual approach of static historical reference conditions.  相似文献   
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Biotrophic pathogens, such as the related maize pathogenic fungi Ustilago maydis and Sporisorium reilianum, establish an intimate relationship with their hosts by secreting protein effectors. Because secreted effectors interacting with plant proteins should rapidly evolve, we identified variable genomic regions by sequencing the genome of S. reilianum and comparing it with the U. maydis genome. We detected 43 regions of low sequence conservation in otherwise well-conserved syntenic genomes. These regions primarily encode secreted effectors and include previously identified virulence clusters. By deletion analysis in U. maydis, we demonstrate a role in virulence for four previously unknown diversity regions. This highlights the power of comparative genomics of closely related species for identification of virulence determinants.  相似文献   
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We sequenced and annotated the genome of the filamentous fungus Fusarium graminearum, a major pathogen of cultivated cereals. Very few repetitive sequences were detected, and the process of repeat-induced point mutation, in which duplicated sequences are subject to extensive mutation, may partially account for the reduced repeat content and apparent low number of paralogous (ancestrally duplicated) genes. A second strain of F. graminearum contained more than 10,000 single-nucleotide polymorphisms, which were frequently located near telomeres and within other discrete chromosomal segments. Many highly polymorphic regions contained sets of genes implicated in plant-fungus interactions and were unusually divergent, with higher rates of recombination. These regions of genome innovation may result from selection due to interactions of F. graminearum with its plant hosts.  相似文献   
8.
River/floodplain restoration programs are often based on type-specific reference conditions of the respective river section. Most large rivers in the industrialized world are heavily degraded and lack near-natural reference sites. For that reason, historical analyses of the pre-channelization state have been used to define adequate restoration targets at the Austrian Danube section in the Alluvial Zone National Park downstream from Vienna. This study compares new reference data derived from 3D-reconstructions in the form of digital terrain models from two historical Danube river landscapes: the Machland floodplain in 1812 and the former floodplain in Vienna in 1849. The focus is on the original hydrological surface and subsurface connectivity of the river-floodplain systems related to different flow situations. Here, we use water cover, shorelines, depth of the groundwater table and inundation depth as surrogates for measuring connectivity. The results show that the two study sites naturally constituted extreme formations of the Austrian anabranching Danube sections. The Machland section was one of the narrowest floodplains but highly dynamic, and the Vienna/Lobau section was one of the broadest, whereby only the central part close to the main channel originally showed high morphological turnover. The analyses of hydrological surface connectivity relative to total floodplain extension reveal significant differences due to their basic geological and geographical conditions. Despite these basic differences, both study sites – in absolute numbers – showed similar extensions of the water bodies at low and mean flow. The depths of the groundwater table related to mean flow were also comparable. Prior to channelization, the groundwater conditions in both floodplains favored the formation of wet to moist alluvial forest communities.  相似文献   
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Zusammenfassung Durch Kombination verschiedener Pathotypen vonPhytophthora infestans (Mont.) de Bary, 1985 im Freiland gesammelt, mit ?lteren Isolaten aus der Sammlung des Institutes wurde die M?glichkeit der Oosporenbildung geprüft. Bisher konnte bei zwei Kombinationen Oosporen-bildung beobachter werden. Daraus kann man schliessen, dass nunmehr auch in der Bundesrepublik Deutschland beide Paarungstypen vonP. infestants vorkommen.
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