Root-knot nematodes (RKN) are major pest of olive tree (Olea europaea ssp. europaea), especially in nurseries and high-density orchards. Soil samples were collected from main olive growing areas of Morocco, to characterize Meloidogyne species and to discuss the contribution of biotic and abiotic factors in their spatial distribution.
Results
RKN were found in 159 soil samples out of 305 from nurseries (52.1% occurrence) and in 11 out of 49 soil samples from orchards (23.2% occurrence). Biochemical and molecular characterisation (PAGE esterase and SCAR) revealed the dominance of M. javanica both in nurseries and orchards with minor presence of M. incognita only in nurseries, and M. arenaria in only one nursery. RKN were distributed on aggregated basis. Frequent presence of M. javanica in orchards might have come from nurseries. In contrast, the detection of M. incognita in nurseries alone suggests that this species could not reproduce in orchards because of either the competition with other plant-parasitic nematodes or unfit local habitats. The impact of environmental variables (climate, habitat origin and physicochemical characteristics of the substrates) on the distribution of Meloidogyne species is also discussed.
Conclusion
Olive nurseries in Morocco are not able to guarantee the safety of rooted plants. As a result, olive production systems are exposed to strong RKN invasion risks. Consequently, the use of healthy substrates in nurseries may prevent plant-parasitic nematode induction in orchards.
ABSTRACT: The effects of the different catching methods (gillnet, longline, harpoon) on sensory, chemical (pH, total volatile base nitrogen, K -value) and microbiological (total viable count [TVC]) changes in pike perch Sander lucioperca stored in ice were investigated. The same soaking time was used for both gillnet and longline fishing. The catching method had considerable influence on the freshness quality of pike perch. The acceptable shelf life was 15 days for pike perch caught by gillnet, and 22 days for longline and harpoon. The initial concentrations of inosine monophosphate (2.4 μmol/g) in pike perch caught by gillnet were significantly lower ( P < 0.05) than longline (4.1 μmol/g), and especially by harpoon (16.7 μmol/g). However, the initial K -values for fish caught by harpoon were significantly ( P < 0.05) lower (24.36%) than fish caught by longline and gillnet (57.69%, 64.41%, respectively). The average K , Ki, G and H -values at rejection day in terms of sensory assessment were approximately 90, 98, 156 and 40%, respectively, for all catching methods during ice storage. However, TVC reached 7.0 log cfu/g after approximately 11 days of storage for fish caught by gillnet, 19 days for fish caught by longline and 8 days for fish caught by harpoon. The result of this study suggests that the best catching method for preserving the freshness of pike perch is longline, based on the data obtained from the sensory and microbiological analysis. 相似文献
Growth and ontogeny of digestive function were studied in pikeperch (Sander lucioperca) larvae weaned on artificial food at different ages. Three weaning treatments initiated respectively on day 9 (W9), day 15
(W15) or day 21 (W21) post-hatching (p.h.) were compared with a control group, fed Artemia nauplii from first feeding until the end of the rearing trial on day 36 p.h. The digestive enzyme activities and the ontogeny
of digestive structures were investigated using enzymatic assays and histological methods. Growth of pikeperch larvae was
significantly affected by precocious weaning. Pancreatic (trypsin and amylase) and intestinal (leucine-alanine peptidase,
leucine aminopeptidase N and alkaline phosphatase) enzyme activities were detected from hatching onwards, increased at the
moment of first feeding and then decreased. Pepsin secretion occurred at day 29 p. h. only, concurrently with the stomach
development and differentiation of gastric glands. In the early weaning group (W9) the maturation process of intestinal enterocytes
seems to be impaired and/or delayed and several signs of malnutrition were recorded. Except for alkaline phosphatase activity,
no differences in enzyme activities and development of digestive structures were observed among the control, W21, and W15
groups. Moreover, at the end of the experiment, no differences in proteolytic activities were observed among larvae from the
different treatments, indicating that, in surviving individuals, the digestive structures were properly developed and the
larvae had acquired an adult mode of digestion. Based on the artificial diet used, our results suggested that pikeperch larvae
can be weaned from day 15 p.h. without significant adverse effect on digestive capacities (except for alkaline phosphatase)
or development of digestive tract, while earlier weaning impaired the onset of the maturation processes of the digestive system,
both in terms of morphological structures and enzymatic activities. 相似文献
Soil compaction is one of the major problems facing modern agriculture. Overuse of machinery, intensive cropping, short crop rotations, intensive grazing and inappropriate soil management leads to compaction. Soil compaction occurs in a wide range of soils and climates. It is exacerbated by low soil organic matter content and use of tillage or grazing at high soil moisture content. Soil compaction increases soil strength and decreases soil physical fertility through decreasing storage and supply of water and nutrients, which leads to additional fertiliser requirement and increasing production cost. A detrimental sequence then occurs of reduced plant growth leading to lower inputs of fresh organic matter to the soil, reduced nutrient recycling and mineralisation, reduced activities of micro-organisms, and increased wear and tear on cultivation machinery. This paper reviews the work related to soil compaction, concentrating on research that has been published in the last 15 years. We discuss the nature and causes of soil compaction and the possible solutions suggested in the literature. Several approaches have been suggested to address the soil compaction problem, which should be applied according to the soil, environment and farming system.
The following practical techniques have emerged on how to avoid, delay or prevent soil compaction: (a) reducing pressure on soil either by decreasing axle load and/or increasing the contact area of wheels with the soil; (b) working soil and allowing grazing at optimal soil moisture; (c) reducing the number of passes by farm machinery and the intensity and frequency of grazing; (d) confining traffic to certain areas of the field (controlled traffic); (e) increasing soil organic matter through retention of crop and pasture residues; (f) removing soil compaction by deep ripping in the presence of an aggregating agent; (g) crop rotations that include plants with deep, strong taproots; (h) maintenance of an appropriate base saturation ratio and complete nutrition to meet crop requirements to help the soil/crop system to resist harmful external stresses. 相似文献
Fig has been a typical fruit component of the health-promoting Mediterranean diet for a very long time. Phytochemical characters and antioxidant capacity of green-, yellow-, brown-, purple-, and black-fruited fig (Ficus carica L.) accessions were investigated. In this study, total phenolics (TP), total anthocyanins (TA), fructose (FRUC), glucose (GLUC), sucrose (SUC), and variables (such as L*, a*, C*, and hue°) describing fruit skin colors were examined. Also, the antioxidant capacity (TAC) of fig fruits was determined by the ferric reducing antioxidant power (FRAP) assay. Antioxidant capacity was significantly correlated with the polyphenol and anthocyanin (r = 0.74 and 0.63, respectively) contents of fruits. Black fig accessions had the highest TAC (range of 7.9–16.1, mean 12.4 Fe2+ mmol/kg FW), TA (range of 32.3–356.0, mean 128.4 μg cy-3-rutinoside/g FW), and TP content (range of 69.1–220.0, mean 118.9 mg GAE/100 g FW). These black-fruited accessions had 2-fold greater TAC, 15-fold greater TA, and 2.5-fold greater TP than green and yellow fig accessions. However, the FRUC, GLUC, and SUC content of brown and purple fig accessions were higher than those of other color groups. The predominant sugars present were fructose (∼56%) and glucose (∼43%), as determined by HPLC. 相似文献
Allergic diseases occur in most mammals, although some species such as humans, dogs and horses seem to be more prone to develop allergies than others. In horses, insect bite hypersensitivity (IBH), an allergic dermatitis caused by bites of midges, and recurrent airway obstruction (RAO), a hyperreactivity to stable born dust and allergens, are the two most prevalent allergic diseases. Allergic diseases involve the interaction of three major factors: (i) genetic constitution, (ii) exposure to allergens, and (iii) a dysregulation of the immune response determined by (i) and (ii). However, other environmental factors such as infectious diseases, contact with endotoxin and degree of infestation with endoparasites have been shown to influence the prevalence of allergic diseases in humans. How these factors may impact upon allergic disease in the horse is unknown at this time. The 3rd workshop on Allergic Diseases of the Horse, with major sponsorship from the Havemeyer Foundation, was held in Hólar, Iceland, in June 2007 and focussed on immunological and genetic aspects of IBH and RAO. This particular venue was chosen because of the prevalence of IBH in exported Icelandic horses. The incidence of IBH is significantly different between Icelandic horses born in Europe or North America and those born in Iceland and exported as adults. Although the genetic factors and allergens are the same, exported adult horses show a greater incidence of IBH. This suggests that environmental or epigenetic factors may contribute to this response. This report summarizes the present state of knowledge and summarizes important issues discussed at the workshop. 相似文献
1.?The study was conducted to determine the effects of dietary supplementation of enzyme and oregano essential oil at two levels, alone or together, on performance, digestive enzyme, nutrient digestibility, lipid metabolism and immune response of broilers fed on wheat–soybean meal based diets.
2.?The following dietary treatments were used from d 0 to 21. Diet 1 (control, CONT): a commercial diet containing no enzyme or oregano essential oil, diet 2 (ENZY): supplemented with enzyme, diet 3 (EO250): supplemented with essential oil at 250 mg/kg feed, diet 4 (EO500): supplemented with essential oil at 500 mg/kg feed, diet 5 (ENZY + EO250): supplemented with enzyme and essential oil at 250 mg/kg, and diet 6 (ENZY + EO500): supplemented with enzyme and essential oil at 500 mg/kg.
3.?Birds fed on diets containing ENZY, EO250 and ENZY + EO250 had significantly higher weight gain than those given CONT diet from d 0 to 7. No significant effects on feed intake, feed conversion ratio, mortality, organ weights except for jejunum weight and intestinal lengths was found with either enzyme or essential oil, alone or in combination, over the 21-d growth period. The supplementation of essential oil together with enzyme decreased jejunum weight compared with essential oil alone.
4.?Supplementation with enzyme significantly decreased viscosity and increased dry matter of digesta, but did not alter pH of digesta. There was no effect of essential oil alone at either concentration on viscosity, dry matter or pH of digesta. A significant decrease in viscosity of digesta appeared when essential oil was used with together enzyme.
5.?The supplementation of essential oil at both levels with or without enzyme significantly increased chymotrypsin activity in the digestive system, and improved crude protein digestibility.
6.?The higher concentration of essential oil with and without enzyme significantly increased serum total cholesterol concentrations. No significant effect on immune response was found with either enzyme or essential oil, alone or together.
7.?Enzymes and essential oil had different modes of actions. The supplementation of enzyme with essential oil in diets is likely more effective in view of performance, nutrient digestibility, enzyme activities and immune system. 相似文献
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