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Although birds infected with avian polyomavirus (APV) subclinically could be a source of infection, no epidemiological studies of APV in psittacine birds have been reported in Japan. In the present study, we investigated subclinical morbidity rate of APV in imported and domestically bred psittacine birds by polymerase chain reaction (PCR). Of 402 live birds from which blood or feather samples were taken between April, 2003 and March, 2004, 11 (2.7%) were found to be APV positive. The DNA sequences of the APV t/T antigen region were determined for five APV-positive randomly selected samples and were found to be conserved.  相似文献   
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A 5-month-old female Citron-crested Cockatoo (Cacatua sulphurea citrinocristata) that was born and hand-reared in Japan died with suspected proventricular dilatation disease (PDD). Macroscopic and microscopic examinations of the bird revealed characteristic features of PDD, i.e., distention of the proventriculus and infiltration of lymphocytes and plasma cells in ganglia of various organs and in central and peripheral nerves. A linkage of this PDD case to infection with avian bornavirus (ABV) was documented by RT-PCR amplification of the virus genomes from the affected bird. Phylogenetic analysis revealed that the ABV identified in this study clustered into the genotype 2, which is one of the dominant ABV genotypes worldwide. To the best of our knowledge, this is the first report of a natural case of PDD associated with ABV infection in Japan.  相似文献   
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Fine root dynamics and root architecture were studied in the organic and mineral soil layers of a Cryptomeria japonica plantation. Fine root biomass (<1 mm) showed seasonal changes whereas fine root biomass (1–2 mm) was unchanged over the study period. Root tips were grouped into size classes based on root tip diameter, including <0.5, 0.5–1, and 1–2 mm. Root tip density (<1 mm) was significantly correlated with fine root biomass (<1 mm). Root tip density and fine root biomass (<1 mm) increased in summer and decreased in winter, and both showed a similar seasonal pattern. Root tip dynamics influenced fine root dynamics. Root architecture as expressed by branching intensity changed with root tip production and mortality. Branching intensity also showed a similar seasonal pattern of root tip density dynamics. Root tips of both <0.5 and 0.5–1 mm were mainly produced in the organic soil layer, while root tips of 0.5–1 mm were mainly produced in the mineral soil layer. Because of the high RT1 root tip production in the organic soil layer, branching intensity was higher in the organic soil than in the mineral soil layer during summer. Root tip dynamics influenced fine root dynamics and the architecture of root systems in both organic and mineral soil layers.  相似文献   
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Mentha spicata L. (spearmint) was collected from different sub-tropical and temperate zones of North-West Himalayan region of India. Plants were collected during the flowering stage and essential oil was extracted using Clevenger-type apparatus for 2.5 h and analyzed. GC–MS analysis reveals that carvone was major part which varied between 49.62%–76.65%, second major component was limonene that varied between 9.57%–22.31%. 1,8-cineole varied between 1.32%–2.62%, whereas trans-carveol varied between 0.3%–1.52%. Out of 26 collections, one accession was found rich in carvone (76.65%) along with low limonene content (9.57%). Chemical composition of this accession is described in detail.  相似文献   
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Objective— To compare the biomechanical properties of cervical arthroplasty to a ventral slot procedure and pin‐polymethylmethacrylate (pin‐PMMA) fixation. Sample Population— Fresh cadaveric cervical (C2–T1) spines from 6 large dogs. Methods— Four spinal conditions were studied in each spinal specimen: intact, disk arthroplasty, ventral slot, and fixation with smooth pin‐PMMA at C5–C6 intervertebral space. Axial compression, torsion, flexion–extension, and lateral bending moments were sequentially tested on each specimen for the 4 spinal conditions. Data from the C3–C4, C4–C5, C5–C6, and C6–C7 vertebral motion units (VMUs) were compared among treatments. Results— In axial compression and torsion, the ventral slot procedure allowed significantly less motion than intact, pin‐PMMA, and arthroplasty groups at C5–C6. In lateral bending and flexion–extension, pin‐PMMA had the least motion of C5–C6, followed by the arthroplasty group, intact spine, and ventral slot, all of which were significantly different from each other. Overall, the artificial disk was better able to mimic the behavior of the intact specimens compared with the ventral slot and pin‐PMMA, producing similar displacements in axial compression and rotation in torsion, but more limited motion than intact in flexion–extension and in lateral bending. Conclusion— Cervical spine specimens with an implanted prosthesis have biomechanical behaviors more similar to an intact spine compared with spinal specimens with ventral slot and pin‐PMMA procedures. Cervical arthroplasty may then preserve some of the motion in the affected area after neural decompression while providing distraction. Clinical Relevance— Cervical arthroplasty should be further investigated in vivo to determine if it is a viable alternative to the ventral slot or pin‐PMMA procedures for surgical treatment of cervical diseases in dogs and in particular for treatment of disk‐associated caudal cervical spondylomyelopathy.  相似文献   
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Staining-type hypermelanosis, defined as blind-side melanosis occurring after completion of metamorphosis, reduces commercial value in hatchery-produced flatfishes. Detailed characterization was performed on the stained area of juvenile Japanese flounder Paralichthys olivaceus to physiologically understand this phenomenon. From 80 to 120 days after hatching, juveniles were reared in sandy and sandless tanks. By classifying the staining degree into 7 levels, about 2 times higher occurrence of middle-level staining was reconfirmed in sandless tank (about 80 %) than in sandy tank (about 40 %). In the stained area, we found 3 types of chromatophores (melanophore, xanthophore, and iridophore) and ctenoid scales, which would be typically observed on the normal ocular side. Detailed examination on the melanophores revealed further similarity between the stained area and the normal ocular side, in terms of the distribution at 2 layers (shallower and deeper than scale), and the densities in both layers (about 1000 cells/mm2 above scale and 200 cells/mm2 beneath scale). These results strongly suggest that the staining is a status change in the body surface conditions from the blind side to that on the ocular side, and not a simple darkening caused by disordered proliferation of melanophores on the blind side.  相似文献   
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