排序方式： 共有24条查询结果，搜索用时 15 毫秒
1.
Susan L Steinberg Gerard J Kluitenberg Scott B Jones Nihad E Daidzic Lakshmi N Reddi Ming Xiao Markus Tuller Rebecca M Newman Dani Or J Iwan D Alexander 《Journal of the American Society for Horticultural Science》2005,130(5):767-774
Baked ceramic aggregates (fritted clay, arcillite) have been used for plant research both on the ground and in microgravity. Optimal control of water and air within the root zone in any gravity environment depends on physical and hydraulic properties of the aggregate, which were evaluated for 0.25-1-mm and 1-2-mm particle size distributions. The maximum bulk densities obtained by any packing technique were 0.68 and 0.64 g cm-3 for 0.25-1-mm and 1-2-mm particles, respectively. Wettable porosity obtained by infiltration with water was approximately 65%, substantially lower than total porosity of approximately 74%. Aggregate of both particle sizes exhibited a bimodal pore size distribution consisting of inter-aggregate macropores and intra-aggregate micropores, with the transition from macro- to microporosity beginning at volumetric water content of approximately 36% to 39%. For inter-aggregate water contents that support optimal plant growth there is 45% change in water content that occurs over a relatively small matric suction range of 0-20 cm H2O for 0.25-1-mm and 0 to -10 cm H2O for 1-2-mm aggregate. Hysteresis is substantial between draining and wetting aggregate, which results in as much as a approximately 10% to 20% difference in volumetric water content for a given matric potential. Hydraulic conductivity was approximately an order of magnitude higher for 1-2-mm than for 0.25-1-mm aggregate until significant drainage of the inter-aggregate pore space occurred. The large change in water content for a relatively small change in matric potential suggests that significant differences in water retention may be observed in microgravity as compared to earth. 相似文献
2.
Iwan Wästerlund 《Forest Ecology and Management》1985,11(3):171-189
Five of the most common soils in forest areas in Sweden, viz., coarse sand, fine sand, coarse sandy till, medium sandy till and fine sandy till soil, were compressed at different loadings for 10 min. In the soils studied, it was found that the more fine material there was in the soil, the easier it compacted, and that till soils compacted much more than sediments of the same main particle class. The chosen compression time seemed to correspond well with an equilibrium level of static compaction. Influence of wall friction in compression tubes was found to be negligible for till soils.After compaction the soil cores were sown with Norway spruce (Picea abies (L.) Karst.) or Scots pine (Pinus sylvestris L.) and 16–20 days later the root lengths were recorded. Lengths of primary roots in per cent of the control were found to be almost inverse to the compaction of the soils; Norway spruce was more impeded than Scots pine. Thus, the bulk density alone is a poor indicator of the impact of soil compaction. Growth to 26, 33 and 120 days showed that the extension of secondary roots might be more impeded by compaction than the primary roots. Fertilization increased growth but did not decrease the growth difference between seedlings on unloaded and loaded soil cores. 相似文献
3.
An experimental procedure has been developed for conducting photolysis studies with pesticides adsorbed by soil surfaces. In these experiments, solutions of thidiazuron were sprayed on segments of a 0.5-mm layer of soil covering a glass plate. The soil surface was irradiated using simulated sunlight. The light source was a xenon arc in combination with glass filters that absorbed light of wavelength <290 nm. During irradiation, one half of the sprayed areas was covered to obtain nonirradiated samples. After various time intervals, groups of three irradiated and three non-irradiated segments of the soil were scraped off and extracted; the extracts were analysed. Information was obtained on the photoproducts formed and on the kinetics of photodegradation of thidiazuron. 相似文献
4.
Zainal A. Muchlisin Putri I. Sarah Dhea F. Aldila Kartini Eriani Iwan Hasri Agung S. Batubara Firman M. Nur Mustaqim Mustaqim Cut Ruhul Muthmainnah Abinawanto Abinawanto Martin Wilkes 《Aquaculture Research》2020,51(4):1700-1705
Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non‐permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non‐permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs (p < .05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk. 相似文献
5.
Katja N. Adamik DrMedVet ; Iwan A. Burgener DrMedVet DACVIM DECVIM ; Alan Kovacevic DrMedVet DECVIM ; Sebastian P. Schulze MRCVS Barbara Kohn DrMedVet DECVIM 《Journal of Veterinary Emergency and Critical Care》2009,19(3):247-253
Objective – To determine whether myoglobin (Mb) is a useful prognostic indicator for outcome and to investigate any relationship between Mb and mortality in dogs with gastric dilatation‐volvulus (GDV). Design – Prospective study. Setting – Veterinary teaching hospital. Animals – Seventy‐two dogs with GDV. Interventions – Blood sampling. Measurements and Main Results – Mb levels were measured at the time of diagnosis (Mbt0), 24 hours (Mbt1), and 48 hours (Mbt2) after signs of GDV were recognized. Fifty‐seven dogs survived (group I) and 15 dogs did not survive (group II). Mb t0 differed significantly between groups (P=0.04). Mbt0 in group I ranged from <30 to >700 ng/mL (n=57, median 74 ng/mL), and in group II from 34 to >700 ng/mL (n=15, median 238 ng/mL). Analysis of a receiver operating characteristic curve of Mbt0 suggested that the best single cutpoint would be 168 ng/mL (sensitivity 60.0%, specificity 84.2%). Fifty percent of dogs with Mbt0>168 ng/mL were euthanized, while 88.9% with Mbt0<168 ng/mL survived. Mbt1 and Mbt2 differed significantly between groups I and II. Mbt1 in group I ranged from 32 to >700 ng/mL (n=55, median 123 ng/mL), and Mbt1 in group II ranged from 131 to 643 ng/mL (n=7, median 343 ng/mL) (P=0.006). Mbt2 in group I ranged from 30 to 597 ng/mL (n=54, median 101 ng/mL), and in group II from 141 to >700 ng/mL (n=8, median 203 ng/mL) (P=0.02). Conclusions – In this study, Mbt0 is a moderately sensitive and specific prognostic indicator. Almost 90% of the dogs below the cutpoint survived to discharge, whereas 50% with Mbt0 above the cutpoint did not survive. 相似文献
6.
Dandrieux JR Bornand VF Doherr MG Kano R Zurbriggen A Burgener IA 《American journal of veterinary research》2008,69(10):1279-1285
Objective-To determine whether lymphocyte apoptosis in intestinal mucosae is more common in healthy dogs than dogs with inflammatory bowel disease (IBD) and whether numbers of apoptotic cells increase after successful treatment of affected dogs. Animals-8 dogs with IBD (IBD dogs) and 8 healthy control dogs. Procedures-Biopsy specimens of the duodenum and colon were obtained via endoscopy from dogs with IBD before and after 10 weeks of standard treatment and compared with specimens obtained from control dogs. Expression of activated caspase 3 (Casp3), caspase-cleaved fragment p85 from poly-ADP-ribose polymerase (PARP), and B-cell leukemia/lymphoma 2 (Bcl-2) was measured in the duodenal (villous tip and base) and colonic mucosae. Results-Expression of Casp3 was greater in the duodenal villous tips of control dogs, compared with expression in similar tissues from dogs with IBD before or after treatment. Despite clinical improvement of dogs with IBD, expression of Casp3 did not increase after treatment. Expression of PARP did not differ between groups at any time point. Expression of Bcl-2 was greater at all 3 tissue sites in control dogs, compared with expression at the same sites in dogs with IBD. Furthermore, Bcl-2 expression in duodenal villous tips was higher in dogs with IBD after treatment but was not higher elsewhere. In control dogs, expression patterns for all 3 markers were similar between sites (villous tip > villous base > colon). Conclusions and Clinical Relevance-Expression of Casp3 in lymphocytes in duodenal villous tips was significantly reduced in dogs with IBD, compared with expression in healthy dogs, but no increase was detected following successful treatment of IBD. Increased expression of Bcl-2 may be a potential marker of the success of treatment. 相似文献
7.
Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns on microbes, and ligand recognition results in production of pro-inflammatory cytokines, reactive oxygen and nitrogen intermediates, and upregulation of costimmulatory molecules. The purpose of this study was to test antibodies specific for human or murine TLR2, 3, 4, 5 and 9 for their use in dogs. Twenty-two antibodies were assessed for recognition of canine monocytes (Mo), granulocytes (Gr) or lymphocytes (Ly) by flow cytometry, and results were compared with isotype-matched controls and other antibodies against the same TLR. Nine monoclonal antibodies detected canine leukocyte subpopulations. Antibodies TL2.1 and TL2.3 (specific for human TLR2), HTA125 (specific for human TLR4), as well as 85B152.5 and 19D759.2 (specific for human TLR5) recognized Mo and Gr but not Ly without permeabilization, and putatively cross-react with canine TLR2, 4 and 5, respectively. Antibodies 40C1285.6 and TLR3.7 (specific for human TLR3) as well as 26C593.2 and 5G5 (specific for human and murine TLR9) recognized Mo, Gr and Ly after their permeabilization and putatively cross-react with canine TLR3 and 9, respectively, inside the cell. None of these nine antibodies recognized paraformaldehyde-treated (4%) canine leukocytes but all except 40C1285.6, TLR3.7 and 5G5 recognized methanol-fixed cells, suggesting that they might be useful also in immunohistochemistry. 相似文献
8.
9.
Iwan M Vissers YM Fiedorowicz E Kostyra H Kostyra E Savelkoul HF Wichers HJ 《Journal of agricultural and food chemistry》2011,59(13):7163-7171
Few studies exist on the influence of processing methods on structural changes and allergenic potential of hazelnut proteins. This study focused on the effect of glycation (Maillard reaction) on the immunoreactivity and degranulation capacity of the purified hazelnut 7S globulin, Cor a 11. After heating, the extent of the Maillard reaction, sensitivity to proteolysis, binding of human IgE or rabbit IgG, and degranulation capacity were analyzed. Changes in electrophoretic mobility, amount of free amino groups, and contents of bound sugar and fructosamine indicated that glycation of Cor a 11 occurred at all conditions. Glycation at 37 °C did not influence the specific IgG or IgE binding and was decreased after heating at 60 and 145 °C. Heating, with or without glucose, at 145 °C increased basophil degranulation capacity. The results suggest that glycation of Cor a 11 at 60 and 145 °C may decrease the IgE/IgG binding properties but not the degranulation capacity of basophils. This is possibly related to aggregation of the proteins as a result of the Maillard reaction. 相似文献