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Eva Patricia López‐Damián  José Alfredo Jiménez‐Medina  Miguel Angel Lammoglia  Jaime Arturo Pimentel  Lourdes Teresa Agredano‐Moreno  Chris Wood  Carlos Salvador Galina  Tatiana Fiordelisio 《Anatomia, histologia, embryologia》2018,47(5):i-i

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Variation in the fruit characteristics of local pear (Pyrus spp.) in the Northwest of Iran     

Khoshnood Alizadeh  Soleiman Fatholahi  Jaime A. Teixeira da Silva 《Genetic Resources and Crop Evolution》2015,62(5):635-641

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Characterization of the intrarenal renin‐angiotensin system in cats with naturally occurring chronic kidney disease     

Bianca N. Loureno  Amanda E. Coleman  Roy D. Berghaus  Jaime L. Tarigo  Chad W. Schmiedt  Scott A. Brown 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(2):647

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Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms: Evidence for genetic diversity and population structure     

Adolfo Isla  Mnica Saldarriaga‐Crdoba  Derie E. Fuentes  Romina Albornoz  Denise Haussmann  Jorge Mancilla‐Schulz  Alexis Martínez  Jaime Figueroa  Ruben Avendao‐Herrera  Alejandro Yez 《Journal of fish diseases》2019,42(5):721-737

Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic‐resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty‐two Chilean P. salmonisisolates, as well as the type strain LF‐89^T, were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48% of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF‐89^T and EM‐90 strains, as well as a seven‐isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen.  相似文献   

Accuracy of pairwise methods in the reconstruction of family relationships, using molecular information from turbot (Scophthalmus maximus)     

Silvia Teresa Rodríguez-Ramilo  Miguel Ángel Toro  Jaime Castro  Jesús Fernández 《Aquaculture (Amsterdam, Netherlands)》2007,273(4):434-442

Many estimators and algorithms have been developed to infer the genealogical relationships from molecular marker data when there is no pedigree information. Most pairwise methods provide estimates in a continuous range that needs to be converted into genealogical relationships (namely full-sibs, half-sibs and unrelated) if there is a previous knowledge of the population structure. Transformations are usually based on arbitrary thresholds, but the possibility of correcting the coancestry estimates via explicit pedigree reconstruction methods has been suggested. Using molecular data for ten highly polymorphic microsatellite loci on a population of turbot (Scophthalmus maximus) with a known genealogy, the behaviour of four pairwise marker-based coancestry estimators and the molecular coancestry has been evaluated. The population consisted on 138 families with 4 full-sib individuals each and one family with 8 full-sib individuals (up to 15 half-sib families were also present due to the sharing of parents between some of the full-sibs families). Our results suggested that transforming the pairwise estimators and the molecular coancestry to family relationships through the establishment of thresholds performs slightly better than the explicit pedigree reconstruction method, when accuracy is measured in a pairwise basis. However, if joint relationships between more than two individuals were tested at a time, the threshold methods led to a high percentage of incongruous triads of full-sib individuals, with Mendelian incompatibilities appearing in congruous full-sib families (more than 70% and 60% in our study, respectively). The explicit pedigree reconstruction approach, due to its nature, is free from such problems. Therefore, the pedigree reconstruction approach seems to be a valuable tool to provide a congruent and compatible pedigree when the pairwise marker-based coancestry matrices or the molecular coancestry need to be transformed.  相似文献   

Enriching rotifers with “premium” microalgae. Isochrysis aff. galbana clone T-ISO     

Martia Ferreira  Ana Maseda  Jaime Fbregas  Ana Otero 《Aquaculture (Amsterdam, Netherlands)》2008,279(1-4):126-130

The effect of semi-continuous culture on the nutritional value of microalgae was tested in the rotifer Brachionus plicatilis in short-term enrichment experiments. Isochrysis aff. galbana clone T-ISO was cultured semi-continuously with renewal rates from 10 to 50% of the volume of the culture per day and used to feed the rotifers. After 24 h, dramatic differences in dry weight and protein, lipid and carbohydrate contents were observed in the rotifers depending on the renewal rate applied to the microalgal culture. Rotifers fed T-ISO cultured with low renewal rates showed low dry weight and organic content, whereas rotifers fed microalgae from nutrient-sufficient, high renewal rate cultures showed higher dry weight and increases up to 60% in protein, 35% in lipid and 100% in carbohydrate contents. Feed conversion rate (FCR) and organic FCR decreased with increasing renewal rates, indicating a more efficient assimilation of the microalgal biomass obtained at high growth rates. The fatty acid profile of rotifers reflected that of T-ISO, with maximum content of polyunsaturated fatty acids (PUFAs), n-3 fatty acids and docosahexaenoic acid (DHA) being found in the rotifers fed microalgae from the renewal rate of 40%. Results demonstrate that the biochemical composition of B. plicatilis is strongly modified through the use of semi-continuous cultures of microalgae in short-term enrichment processes. This technique provides an excellent tool to improve the nutritional value of the live feed used in fish larvae cultures.  相似文献   

The politics of water democracy: insights from grassroot struggles in the Ecuadorian Highlands          下载免费PDF全文

Jaime Hoogesteger 《Asia Pacific viewpoint》2017,58(1):74-85

In the past two decades spaces for user participation have been opened within water governance structures at many scales. In this contribution, based on a case study of the Provincial Water Users Federation Interjuntas‐Chimborazo in the Ecuadorian Highlands, I explore how and why formal participation of water users in itself is problematic in terms of democracy. The case shows that for organised peasant water users to work on more democratic water governance, the creation of upscaled federations, alliances, networks and sometimes street protests is crucial to open up spaces in which their interests are represented. This suggests that democracy is not merely about participation, but more importantly, it is about the politics of how democracy is made through conflicts, protests, negotiations and the creation of strategic alliances that challenge the structures and processes through which decisions are taken.  相似文献   

Thinning enhances stool resistance to an extreme drought in a Mediterranean Quercus ilex L. coppice: insights for adaptation     

Domingo  Jose  Zavala  Miguel A.  Madrigal-González  Jaime 《New Forests》2020,51(4):597-613

New Forests - Mediterranean holm oak forests are subjected to chronic seasonal droughts coinciding with the warmest conditions during the summer. Importantly, climate change projections support...  相似文献   

Assessment of molecular detection of Fusarium circinatum in insects and passive spore traps in Pinus radiata plantations     

Jaime Aguayo  Cline Fourrier‐Jeandel  Xavier Capdevielle  Fabrice Vtillard  Dominique Piou  Eugenia Iturritxa  Ccile Robin 《Forest Pathology》2020,50(1)

Fusarium circinatum is the causal agent of pitch canker, a destructive disease that threatens natural and planted pine forests around the world. Although pitch canker has caused problems in Spain and Portugal, concerning Europe as a whole, the fungus is not established across the pine distribution area. Its dispersion by wind and/or insect vectors could nevertheless play a role in the colonization of currently uninfected stands. It is therefore crucial to develop monitoring tools for its detection. To this end, we assessed the molecular detection of the pathogen in environmental samples of bark beetles and passive spore traps, collected in two infected Pinus radiata plantations in Basque country, Spain. The spread pattern of F. circinatum was assessed by an experimental design that included insect and spore traps installed at the centre, at the edge and outside the plots. Our results showed that F. circinatum was detected in both types of samples, at almost all collection dates. In both type of samples, positive detections were mainly found at the centre of the plots, a lower proportion at the edge, and very few outside. This suggests that long‐distance dispersion of Fusarium circinatum does not rely on wind spore dispersal neither on insect flight. Our study also shows that molecular methods are a powerful tool to monitor the pathogen in environmental samples.  相似文献   

Chitosan-Based Films with 2-Aminothiophene Derivative: Formulation,Characterization and Potential Antifungal Activity     

Vernica da Silva Oliveira  Meringela Miranda da Cruz  Gabriela Suassuna Bezerra  Natan Emanuell Sobral e Silva  Fernando Henrique Andrade Nogueira  Guilherme Maranho Chaves  Jos Lamartine Soares Sobrinho  Francisco Jaime Bezerra Mendona-Junior  Bolívar Ponciano Goulart de Lima Damasceno  Attilio Converti  dley Antonini Neves de Lima 《Marine drugs》2022,20(2)

In this study, films of chitosan and 2-amino-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carbonitrile (6CN), a 2-aminothiophene derivative with great pharmacological potential, were prepared as a system for a topical formulation. 6CN-chitosan films were characterized by physicochemical analyses, such as Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and scanning electronic microscopy (SEM). Additionally, the antifungal potential of the films was evaluated in vitro against three species of Candida (C. albicans, C. tropicalis, and C. parapsilosis). The results of the FTIR and thermal analysis showed the incorporation of 6CN in the polymer matrix. In the diffractogram, the 6CN-chitosan films exhibited diffraction halos that were characteristic of amorphous structures, while the micrographs showed that 6CN particles were dispersed in the chitosan matrix, exhibiting pores and cracks on the film surface. In addition, the results of antifungal investigation demonstrated that 6CN-chitosan films were effective against Candida species showing potential for application as a new antifungal drug.  相似文献