Lead poisoning in Canada geese in Delaware   总被引：1，自引：0，他引：1  

G E Bagley  L N Locke  G T Nightingale 《Avian diseases》1967,11(4):601-608

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Aspergillus fumigatus infection in the nasal cavity of a dog: its treatment with amphotericin B   总被引：1，自引：0，他引：1  

L Black  J P Nightingale 《The Veterinary record》1973,92(17):447-450

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Characterization of monoclonal antibodies against avian reovirus S1133 protein sigmaA synthesized in Escherichia coli     

Pai WC  Shien JH  Liu HJ  Lee LH 《Veterinary microbiology》2003,91(4):309-323

Monoclonal antibodies (MAbs) were prepared against avian reovirus S1133 protein sigmaA (esigmaA) synthesized in Escherichia coli. MAbs were characterized and used to develop a diagnostic test. Ten MAbs were selected for competitive binding assay following coupling with horseradish peroxidase. The results indicated that these MAbs delineated two epitopes I and II of esigmaA. An immuno-dot binding assay was used to detect the effect of denaturation on antibody recognition of the epitopes. All MAbs bound to esigmaA in its native form. After denaturation by boiling in SDS and 2-mercaptoethanol, the binding of MAbs recognizing epitope I was fully abolished. However, the reactivity of MAbs recognizing epitope II was not affected. MAbs 31 and 32, recognizing epitopes I and II, respectively, were selected for the cross-reactivity to heterologous reovirus strains. The results suggest that the two epitopes are highly conserved among these virus strains. A MAb capture enzyme-linked immunosorbent assay (ELISA) procedure was developed using MAbs 32 and 31 to detect reovirus protein sigmaA in samples from tendon tissues of infected bird and chicken embryo fibroblast (CEF) cell cultures. Avian reovirus sigmaA antigens in tendon specimens were detected from the inoculated birds as early as 2 days post-inoculation (PI), approximated a peak at 7 days PI, and maintained this until 16 days PI, then decreased gradually. A clear difference in absorbance values between the tendon samples of the avian reovirus- and mock-infected birds is obtained. Positive results were also obtained from avian reovirus-infected CEF and from the tendon tissues of naturally infected broilers. These results indicated that the MAb capture ELISA is a useful methods for the detection of avian reovirus from chickens suspected to have avian reovirus infections.  相似文献   

Quecksilber und Methylquecksilber in Böden und Pflanzen unterschiedlich belasteter Standorte der Slowakei. Mercury and methylmercury in soils and plants of differently contaminated sites in Slovakia     

Renta Dombaiov  Jen‐How Huang  Egbert Matzner 《植物养料与土壤学杂志》2005,168(2):238-240

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Antioxidant capacity of oat (Avena sativa L.) extracts. 1. Inhibition of low-density lipoprotein oxidation and oxygen radical absorbance capacity     

Handelman GJ  Cao G  Walter MF  Nightingale ZD  Paul GL  Prior RL  Blumberg JB 《Journal of agricultural and food chemistry》1999,47(12):4888-4893

Milled oat groat pearlings, trichomes, flour, and bran were extracted with methanol and the fractions tested in vitro for antioxidant capacity against low-density lipoprotein (LDL) oxidation and R-phycoerythrin protein oxidation in the oxygen radical absorbance capacity (ORAC) assay. The oxidative reactions were generated by 2,2'-azobis(2-amidinopropane) HCl (AAPH) or Cu(2+) in the LDL assay and by AAPH or Cu(2+) + H(2)O(2) in the ORAC assay and calibrated against a Trolox standard to calculate Trolox equivalents (1 Trolox equivalent = 1 TE = activity of 1 micromol of Trolox). The antioxidant capacity of the oat fractions was generally consistent with a potency rank of pearlings (2.89-8.58 TE/g) > flour (1.00-3.54 TE/g) > trichome (1.74 TE/g) = bran (1.02-1.62 TE/g) in both LDL and ORAC assays regardless of the free radical generator employed. A portion of the oat antioxidant constituents may be heat labile as the greatest activity was found among non-steam-treated pearlings. The contribution of oat tocols from the fractions accounted for <5% of the measured antioxidant capacity. AAPH-initiated oxidation of LDL was inhibited by the oat fractions in a dose-dependent manner, although complete suppression was not achieved with the highest doses tested. In contrast, Cu(2+)-initiated oxidation of LDL stimulated peroxide formation with low oat concentrations but completely inhibited oxidation with higher doses. Thus, oats possess antioxidant capacity most of which is likely derived from polar phenolic compounds in the aleurone.  相似文献   

Mutations in a human ROBO gene disrupt hindbrain axon pathway crossing and morphogenesis     

Jen JC  Chan WM  Bosley TM  Wan J  Carr JR  Rüb U  Shattuck D  Salamon G  Kudo LC  Ou J  Lin DD  Salih MA  Kansu T  Al Dhalaan H  Al Zayed Z  MacDonald DB  Stigsby B  Plaitakis A  Dretakis EK  Gottlob I  Pieh C  Traboulsi EI  Wang Q  Wang L  Andrews C  Yamada K  Demer JL  Karim S  Alger JR  Geschwind DH  Deller T  Sicotte NL  Nelson SF  Baloh RW  Engle EC 《Science (New York, N.Y.)》2004,304(5676):1509-1513

The mechanisms controlling axon guidance are of fundamental importance in understanding brain development. Growing corticospinal and somatosensory axons cross the midline in the medulla to reach their targets and thus form the basis of contralateral motor control and sensory input. The motor and sensory projections appeared uncrossed in patients with horizontal gaze palsy with progressive scoliosis (HGPPS). In patients affected with HGPPS, we identified mutations in the ROBO3 gene, which shares homology with roundabout genes important in axon guidance in developing Drosophila, zebrafish, and mouse. Like its murine homolog Rig1/Robo3, but unlike other Robo proteins, ROBO3 is required for hindbrain axon midline crossing.  相似文献   

Creating favorable geometries for directing organic photoreactions in alkanethiolate monolayers     

Kim M  Hohman JN  Cao Y  Houk KN  Ma H  Jen AK  Weiss PS 《Science (New York, N.Y.)》2011,331(6022):1312-1315

The products of photoreactions of conjugated organic molecules may be allowed by selection rules but not observed in solution reactions because of unfavorable reaction geometries. We have used defect sites in self-assembled alkanethiolate monolayers on gold surfaces to direct geometrically unfavorable photochemical reactions between individual organic molecules. High conductivity and stochastic switching of anthracene-terminated phenylethynylthiolates within alkanethiolate monolayers, as well as in situ photochemical transformations, have been observed and distinguished with the scanning tunneling microscope (STM). Ultraviolet light absorbed during imaging increases the apparent heights of excited molecules in STM images, a direct manifestation of probing electronically excited states.  相似文献   

Evaluation of a focused assessment with sonography for trauma protocol to detect free abdominal fluid in dogs involved in motor vehicle accidents     

Boysen SR  Rozanski EA  Tidwell AS  Holm JL  Shaw SP  Rush JE 《Journal of the American Veterinary Medical Association》2004,225(8):1198-1204

OBJECTIVE: To establish a focused assessment with sonography for trauma (FAST) protocol in dogs, determine whether FAST can be performed by veterinary clinicians without extensive ultrasonographic experience, and assess the frequency of free fluid (as determined via FAST) in the abdominal cavity of dogs following motor vehicle accidents (MVAs). DESIGN: Prospective study. ANIMALS: 100 client-owned dogs evaluated within 24 hours of an MVA. PROCEDURE: Dogs were placed in lateral recumbency for the FAST examination. To detect fluid in the abdomen, 2 ultrasonographic views (transverse and longitudinal) were obtained at each of 4 sites (just caudal to the xiphoid process, on the midline over the urinary bladder, and at the left and right flank regions). RESULTS: In the 100 dogs evaluated via FAST, free abdominal fluid was detected in 45 dogs. In 40 of those 45 dogs, abdominocentesis was performed; hemoperitoneum and uroperitoneum were diagnosed in 38 and 2 dogs, respectively. Compared with dogs that had no free abdominal fluid detected via FAST, dogs that had free abdominal fluid detected via FAST had significantly higher heart rates and serum lactate concentrations and significantly lower PCVs and total solid concentrations. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate that FAST is a simple and rapid technique that can be performed on dogs in an emergency setting to detect intra-abdominal free fluid and can be performed by veterinary clinicians with minimal previous ultrasonographic experience.  相似文献   

Public health. Getting HIV treatment to the most people     

S Lynch  N Ford  G van Cutsem  H Bygrave  B Janssens  T Decroo  I Andrieux-Meyer  T Roberts  S Balkan  E Casas  C Ferreyra  M Bemelmans  J Cohn  P Kahn  E Goemaere 《Science (New York, N.Y.)》2012,337(6092):298-300

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Investigating the microstructure of plant leaves in 3D with lab-based X-ray computed tomography     

Andrew W. Mathers  Christopher Hepworth  Alice L. Baillie  Jen Sloan  Hannah Jones  Marjorie Lundgren  Andrew J. Fleming  Sacha J. Mooney  Craig J. Sturrock 《Plant methods》2018,14(1):99

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