Interactions between different media and follicle‐stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacu Linneaus, 1758)          下载免费PDF全文

GL Lima  VB Luz  LF Lima  RMP Rocha  SV Castro  TS Castelo  APR Rodrigues  JR Figueiredo  AR Silva 《Reproduction in domestic animals》2018,53(4):880-888

The aim was to verify the effect of follicle‐stimulating hormone (FSH) supplementation to α‐MEM+ or TCM199+ media on the in vitro development of ovarian preantral follicles (PFs) derived from collared peccaries. Ovaries (n = 5 pairs) were collected and divided into fragments destined to control group (non‐cultured) or treatments that were cultured for 7 days. The PFs morphology, growth and activation were evaluated by classical histology. The immunohistochemistry markers Ag‐NOR and PCNA were used for nuclear proliferation analysis, and the picrosirius red labelling was used for ovarian extracellular matrix (ECM) evaluation. After 7‐day culture, only the TCM199+ treatment maintained the proportion of intact PFs similar to day 1 (63.2%), but no differences were found among treatments (p > .05). In addition, a significant increase in the growing follicles proportion was verified for all the treatments, indicating follicular activation (p > .05). By the Ag‐NOR analysis, only the TCM199+/FSH maintained the nuclear proliferation similar to the first day (p > .05). The picrosirius red staining revealed that the ECM remained intact in all the treatments (p > .05). We suggest the use of TCM199+ medium supplemented of FSH for the in vitro development of peccaries PFs under 7‐day culturing conditions.  相似文献   

Early resynchronization protocols for goats: Progestogens can be used prior to an early pregnancy diagnosis without affecting corpus luteum functionality     

Isabel Oliveira Cosentino  Mario Felipe Alvarez Balaro  Felipe Seabra Cardoso Leal  Lucas de Figueiredo Cardoso Barbosa  Fernanda Martins Gonçalves  Gabriel Feliciano Felizardo  Marina Monteiro Netto  Felipe Zandonadi Brandão 《Reproduction in domestic animals》2020,55(11):1655-1659

This study aimed to evaluate the exogenous progesterone (P4) effect on the luteal function from Day 16 to Day 21 of the oestrous cycle in inseminated goats with unknown pregnancy status. A total of 54 does passed through a short progestin-based synchronization protocol and, on Day 16 of the following oestrous cycle, 27 does received a new P4 device which was retained until Day 21. Blood samples were collected daily from all does during this period, as well as on Day 24. Pregnancy diagnoses were performed on Day 30. Serum P4 values from 26 animals (G_NPSP: Group of non-pregnant does with second sponge: n = 8; G_NPNSP: Group of non-pregnant does without second sponge: n = 6; G_PSP: Group of pregnant does with second sponge: n = 5; G_PNSP: Group of pregnant does without second sponge: n = 7) were determined by radioimmunoassay commercial kits. No P4 differences were found between groups (G_NPSP: 3.1 ± 2.8; 1.7 ± 1.8; 0.4 ± 1.0; and 0.0 ± 0.0 vs. G_NPNSP: 4.4 ± 1.8; 3.0 ± 2.2; 0.8 ± 0.8; and 0.0 ± 0.0 or G_PSP: 4.2 ± 1.0; 3.4 ± 0.6; 3.3 ± 1.6; 3.2 ± 0.9; 3.6 ± 1.2; 3.5 ± 1.3; 2.7 ± 1.3 vs. G_PNSP: 4.4 ± 1.6; 3.6 ± 1.5; 3.7 ± 1.5; 3.8 ± 1.4; 3.2 ± 1.2; 3.1 ± 1.2; 3.6 ± 1.1; D16, D17, D18, D19, D20, D21, D24, respectively) or for the interaction of group and time. In conclusion, a second progestogen device had no effect on luteolysis or early pregnancy in the following oestrous cycle.  相似文献   

Comparison of selection methods among and within full-sibling progenies in Urochloa humidicola     

Yasmin Vasques Berchembrock  Ulisses José de Figueiredo  José Airton Rodrigues Nunes  Cacilda Borges do Valle  Sanzio Carvalho Lima Barrios 《Grass and Forage Science》2020,75(2):145-152

Hybridization in the Urochloa humidicola breeding programme allowed to explore the genetic variability of the apomictic sources and, thus, to obtain new sexual and apomictic parents with compatible ploidy and/or superior progenies. However, given the high variability arising from crosses, there is a need to adopt efficient selection strategies among and within progenies. The aim of this study was to compare the selection methods among U. humidicola progenies with (t-BLUP) and without (BLUP) the weighting based on prediction error and subsequent selection within progenies by individual simulated (BLUP_IS) or optimal individual BLUP. We evaluated seventy-one U. humidicola full-sib progenies from biparental crosses between nine sexual and ten apomictic parents. The experiment was set up in an incomplete block design with 64 blocks of 15 plots each, consisting of individual plants. Seven consecutive cuts were made and the traits total dry-matter, leaf dry-matter and regrowth scores were measured. Genetic variance was expressive for all traits. The selection procedure among progenies of U. humidicula based on t-BLUP proved to be more advantageous regarding the use of BLUP not weighted by the prediction error, resulting in higher selection gains, taking as reference the optimal procedure of the individual BLUP. Furthermore, the use of BLUP_IS allowed an optimized selection of hybrids, allowing the breeder to explore with variable selection intensity the genetic variability within the selected full-sib progenies based on relative genetic merit.  相似文献   

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Ana Cristina Silva  Joana Henriques  Eugnio Diogo  Ana Paula Ramos  Helena Bragana 《Forest Pathology》2020,50(1)

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Use of genomic and phenotypic selection in lines for prediction of testcrosses in maize II: grain yield and plant traits     

Gustavo Vitti Môro  Mateus Figueiredo Santos  Cláudio Lopes de SouzaJr. 《Euphytica》2017,213(6):128

Plant breeders have been trying to predict the performance of hybrids based on their parental performance. One application of molecular markers is its use in selection. The objectives were to map quantitative trait loci (QTL) and verify its congruence in maize lines and in their testcrosses and verify the possibility to select testcrosses from the predicted means of the lines by using information from markers. Two-hundred and fifty six lines and the testcrosses of these lines with two testers were evaluated in six environments, considering grain yield, plant lodging, days to anthesis and silking, anthesis-silking interval, plant and ear height and ear placement. QTL were mapped in the lines and in testcrosses and the predicted means of the lines were computed based on QTL effects and in all markers of the genome. The congruence of QTL detected in the lines and testcrosses were small for all traits. The correlations between the predicted means of the lines and the phenotypic means of the testcrosses ranged from low for grain yield to moderate for cycle and stature traits. The highest coincidences of the lines and selected testcrosses were observed for cycle and stature traits and the lowest for grain yield. Even by using molecular markers information, it is only possible to predict the testcrosses performance from the lines information to less complex traits and with reduced dominance effect. For complex traits and with pronounced dominance effect, information of markers must be obtained directly in the testcrosses, so they can be used for selection.  相似文献   

Relations between relative evapotranspiration and predawn leaf water potential in soybean grown in several locations     

B. Itier  D. Flura  K. Belabbes  P. Kosuth  G. Rana  L. Figueiredo 《Irrigation Science》1992,13(3):109-114

Summary The measurement of water consumption in the field is normally restricted to research purposes, although the development of practical field criteria for timing water application is required to improve crop productivity. To develop such criteria irrigation experiments on Soybean were conducted from flowering to grain filling at four locations which differed in their soil properties and the convective contribution of their climates to potential evapotranspiration. The energy balance, predawn leaf water potential (PLWP), soil moisture depletion, and a crop water stress index (CWSI) based on foliage temperature were measured. The range of soil, atmospheric, phenological and irrigation conditions, produced a common, linear relation between relative evapotranspiration (rET) and the logarithm of -PLWP. Correlation with the temperature based CWSI was weak. A similar relation with PLWP for other C₃ plants was also derived from data in the literature. This relation could be helpful for irrigation scheduling once the critical values of rET for crop productivity are known.  相似文献   

Extrinsic and intrinsic factors influence daily activity of a Mediterranean cyprinid     

Joana Martelo  Gary D. Grossman  M. Filomena Magalhães 《Ecology of Freshwater Fish》2013,22(2):307-316

Factors shaping individual behavior remain poorly understood for European cyprinids. We examined the effects of extrinsic (temperature and year) and intrinsic factors (body size and shoal size) on the daily activity of chub Squalius torgalensis, a critically endangered cyprinid found in the Torgal stream, Portugal. We quantified chub behaviors while snorkeling, using instantaneous sampling of focal individuals, in spring 2009 and 2010. We used Principal Component Analysis to describe individual behavior and assessed competing models relating individual behavior to explanatory variables using Akaike's Information Criterion. Year had a strong influence on chub behavior, with individuals spending more time drift foraging and orienting in 2009, whereas in 2010 they spent more time benthic foraging and cruising. Shoal size did not influence behaviors in 2009, but in 2010 individuals in small shoals foraged benthically and searched with greater frequency than those in large shoals. Individuals increased cruising and decreased searching as they grew in length, but the opposite trend was observed as temperature increased. Our results indicate that both extrinsic and intrinsic factors influence daily activity of chub, with individual behaviors varying between years, and depending upon shoal size, body size and temperature. This suggests that chub display high levels of individual behavioral flexibility, which may be important for fish inhabiting harsh and highly variable Mediterranean streams. Conservation efforts for this species will benefit by integrating behavioral information into management strategies.  相似文献   

Chemical restraint of peccaries with tiletamine/zolazepam and xylazine or tiletamine/zolazepam and butorphanol   总被引：3，自引：0，他引：3  

Selmi AL  Mendes GM  Figueiredo JP  Guimarães FB  Selmi GR  Bernal FE  McMannus C  Paludo GR 《Veterinary anaesthesia and analgesia》2003,30(1):24-29

Objective To evaluate the use of a combination of tiletamine/zolazepam and xylazine (TZX) in collared and white‐lipped peccaries and to compare its efficacy as an anesthetic technique with that of tiletamine/zolazepam and butorphanol (TZB). Study design Prospective experimental trial. Animals Seven white‐lipped peccaries (Tayassu pecari) (four females and three males) and four collared peccaries (Tayasu tajacu) (two males and two females). Methods Animal immobilization was attempted with TZX and TZB (IM) on two different occasions. Heart and respiratory rates (HR, RR), rectal temperature (RT), sedation, muscle relaxation, posture, auditory response and analgesia were evaluated every 15 minutes during immobilization. Induction, anesthesia, standing and walking time were determined after drug administration. Results Doses for white‐lipped peccaries were 1.23 ± 0.26 mg kg^?1 (mean ± SD) of TZ and 1.23 ± 0.26 mg kg^?1 of X, and 1.46 ± 0.09 mg kg^?1 of TZ and 0.14 ± 0.008 mg kg^?1 of B; doses for collared peccaries were 1.51 ± 0.29 mg kg^?1 of TZ and 1.51 ± 0.29 mg kg^?1 of X and 1.68 ± 0.02 mg kg^?1 of TZ and 0.17 ± 0.002 mg kg^?1 of B. In white‐lipped peccaries, both drug combinations provided a smooth induction and good immobilization for more than an hour. Anesthesia and standing times were significantly longer in animals given TZB, whereas walking time was significantly longer in animals given TZX. A significant decrease in HR was observed with both treatments. Respiratory rate decreased significantly with TZX, but the rate remained higher than with TZB. Induction and recovery quality in white‐lipped peccaries was better with TZB than with TZX. Neither protocol provided adequate immobilization in collared peccaries. Conclusion and clinical relevance At the doses described, TZB is effective in providing a long period of immobilization, whereas TZX is adequate for short to medium immobilization in white‐lipped peccaries. Neither drug combination was effective in collared peccaries at the doses given.  相似文献   

Variability of resistance in goats infected with Haemonchus contortus in Brazil     

Costa CA  Vieira LD  Berne ME  Silva MU  Guidoni AL  Figueiredo EA 《Veterinary parasitology》2000,88(1-2):153-158

The variability between and within breeds with respect to nematode egg counts (EPG), packed cell volume (PCV) and hemoglobin (Hb) was studied in 36 yearling female goats of the Caninde (15), Bhuj (6) and Anglo-Nubian (15) breeds, exposed to Haemonchus contortus. Nematode-free goats were turned to a contaminated paddock in late February. From then on, fecal egg per gram counts (EPG), packed cell volume (PCV) and hemoglobin (Hb) were determined at 2-week intervals up to Week 18. The EPG, transformed as [log(EPG + 75)], varied (P < 0.01) between goats within breeds and between weeks of exposure, but not between goat breeds (P > 0.05). PCV and Hb were affected by goat breeds (P < 0.05), by goats within breeds (P < 0.01) and by weeks of exposure (P < 0.01). Anglo-Nubians had higher (P < 0.01) PCV and Hb than Caninde; Bhuj had intermediate values. There were two EPG rises; one between Weeks 6 and 10 and the other between Weeks 14 and 16. The within breed variability was marked during the EPG rise on Week 6, when individual egg counts ranged from 130 to 2500. The EPG rises coincided with drops in Hb. PCV presented a similar trend, though not as marked. Haemonchus was responsible for more than 95% of nematode eggs counted. Considering the goat as experimental unit, the correlation coefficients (r) were: -0.45, P = 0.0064, between log(EPG + 75) and PCV; and -0.53, P = 0.0009, between log(EPG + 75) and Hb. The negative correlation between egg counts and blood values suggested breed differences in PCV and Hb were related to resistance to H. contortus infection and/or to its effects.  相似文献   

Production and characterization of a panel of monoclonal antibodies for the identification of antigens of Babesia bovis     

Figueiredo JF  Martins MS  Ribeiro MF  Passos LM 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(2):121-126

A panel of monoclonal antibodies was produced and characterized by an indirect fluorescent antibody test (IFAT), an enzyme-linked immunosorbent assay (ELISA) and Western blotting with the aim of identifying antigens of Babesia bovis. After fusion, the resultant hybrids were selected by the IFAT, cloned, maintained in culture in vitro, and cryopreserved in liquid nitrogen. Ten clones producing monoclonal antibodies were found to react against the entire merozoites, three reacted on the surface of the merozoites, and one clone reacted against the polar region of the merozoites. All monoclonal antibodies reacted in ELISA, with the optical density varying from 0.368 to 0.502 (cut off = 0.022). The bands recognized by the monoclonal antibodies in Western blotting had molecular weights ranging from 162 to 19 kDa. Four clones recognized a single band of 73 kDa, and another four did not react in Western blotting.  相似文献